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BIOTROPIA - The Southeast Asian Journal of Tropical Biology
Published by Southeast Asian Regional Centre for Tropical Biology
ISSN : 02156334     EISSN : 1907770X     DOI : http://dx.doi.org/10.11598/btb
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary
BIOTROPIA, The Southeast Asian Journal of Tropical Biology, is a scientific publication of the Southeast Asian Ministers of Education Organization (SEAMEO) – Southeast Asian Regional Center for Tropical Biology (BIOTROP). It publishes articles in the broad areas of tropical ecosystems and environmental impacts, biodiversity conservation and sustainable development and biotechnology.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 3 Documents
 1 
Search results for , issue "No. 17 (2001)" : 3 Documents clear
CHARACTERIZATION OF THREE BENZOATE DEGRADING ANOXYGENIC PHOTOSYNTHETIC BACTERIA ISOLATED FROM THE ENVIRONMENT DWI SURYANTO; ANTONIUS SUWANTO; ANJA MERYANDINI
BIOTROPIA - The Southeast Asian Journal of Tropical Biology No. 17 (2001)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (152.215 KB) | DOI: 10.11598/btb.2001.0.17.166

Abstract

Three  anoxygenic photosynthetic bacteria, DS-1, DS-4 and Cas-13, have been examinated  for  themorphological and physiological  properties. All strains were rod-shape cells with  a swollen terminal  endGram  negative, motile, non-halophilic, non-alkalophilic  and non-acidophilic,  and capable of utilizinbenzoate aerobically and photo-anaerobically. Sequence analysis of part of 16S rRNA genes showed that DS1 and Cas-13 were closely related to Rhodopseudomonas palustris Strain 7 with a similarity of 97%, whereaDS-4 may not be closely related to the former two strains with a similarity of 78% based on the constructephylogenic  tree. Spectral analysis indicated that the three  bacteria  had  bacteriochlorophyl  a  and normaspirilloxanthin series. Growth in medium enriched with vitamin and supplemented with benzoate as their sole C-sources wabetter than in medium without vitamin. Benzoate degradation in medium with vitamin was accelerated. Thability  to grow on benzoate without added vitamins indicated  that  the bacteria were able to synthesize  theown vitamins. Key words: anoxygenic photosynthetic bacteria/ benzoate degradation/ 16S rRNA gene.
ESTABLISHMENT OF NEOCHETINA SPP.: THEIR PATTERN OF LOCAL DISPERSAL AND AGE STRUCTURE AT THE RELEASE SITE SRI WIDAYANTI; KASNO KASNO; ASMARINA S.R.; SUNJAYA SUNJAYA
BIOTROPIA - The Southeast Asian Journal of Tropical Biology No. 17 (2001)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (123.872 KB) | DOI: 10.11598/btb.2001.0.17.168

Abstract

Study on the distribution pattern and age structure ofNeochetina spp. (Coleoptera: Curculionidae) at the release site was conducted to know a) the distribution pattern of the weevil, b) its establishment status, c) its survival rate in the field, and d) relationship between the attack of the weevil and the fungus, Altemaria eichhorniae Nag Rag & Ponappa (Hyphomycetes), in causing damage to water hyacinth. This study was conducted at Situ Bagendit lake, Garut, West Java. A release and recapture method was employed to study the mode and rate of dispersal of the weevils under field conditions. Regular sample collection at two-month interval was done to evaluate the pattern of distribution and to assess the age structure under field condition. Another two months regular observation was done to assess damage severity due to adult weevils and the fungus, A. eichhorniae, on water hyacinth. Results showed, that the weevils seem to disperse actively to all directions following the presence of water hyacinth. The data also showed that the dispersal rate of the weevils was about a few meters a week. Under field conditions at Situ Bagendit lake, the weevils were about evenly distributed throughout water hyacinth mass. The density of the weevils fluctuated from time to time, but the trend slightly increased. The survival rate of the weevils at Situ Bagendit lake was estimated not more than 5%. It was suspected that various limiting factors such as various predators have caused the low population increase under field conditions. The population increase through time confirmed that the weevils have established at Situ Bagendit lake. Field data showed that there were no interaction between the damage severity of the weed caused by both weevils and the fungus.
CHARACTERIZATION OF XYLANASE FROM A XYLANOLYTIC- THERMOPHILIC BACTERIUM ISOLATED FROM GUNUNG PANCAR HOT SPRING, WEST JAVA ANTONIUS SUWANTO; DWI SURYANTO; ANJA MERYANDINI
BIOTROPIA - The Southeast Asian Journal of Tropical Biology No. 17 (2001)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2001.0.17.167

Abstract

A xylanolytic-thermophilic bacterium (IT-08) was isolated from Gunung Pancar Hot Spring after two days of enrichment in Modified Thermus Medium (MTM) supplemented with 0.5% oat spelt xylan. 16S-rRNA sequence analysis indicated that IT-08 resembles Bacillus thermoleovorans, a species of thermophilic bacteria. When grown on xylan containing media, IT-08 produces a thermoactive xylanase. Xylanase from IT-08 was active at temperatures between 40 and 100°C, at pH values between 4.0 and 9.0 with optimum values obtained at 80°C and pH 6.0, respectively. SDS-PAGE and zymogram analysis demonstrated that a crude xylanase complex of IT-08 comprised two active bands with molecular masses of 78 and 60 kDa. Keywords: xylanase / xylanolytic-thermophilic bacteria

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