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BIOTROPIA - The Southeast Asian Journal of Tropical Biology
Published by Southeast Asian Regional Centre for Tropical Biology
ISSN : 02156334     EISSN : 1907770X     DOI : http://dx.doi.org/10.11598/btb
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary
BIOTROPIA, The Southeast Asian Journal of Tropical Biology, is a scientific publication of the Southeast Asian Ministers of Education Organization (SEAMEO) – Southeast Asian Regional Center for Tropical Biology (BIOTROP). It publishes articles in the broad areas of tropical ecosystems and environmental impacts, biodiversity conservation and sustainable development and biotechnology.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 3 Documents
 1 
Search results for , issue "No. 23 (2004)" : 3 Documents clear
PRESERVATION OF GARUT RAMS SPERMATOZOON AS A SOURCE OF MALE GERM PLASM ARIEF BOEDIONO; HERDIS; MUHAMMAD RIZAL'
BIOTROPIA - The Southeast Asian Journal of Tropical Biology No. 23 (2004)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (280.463 KB) | DOI: 10.11598/btb.2004.0.23.200

Abstract

This study was conducted to examine the quality of ejaculated sperm by Garut rams to be used for artificial insemination (AI) and viability of sperm that were collected from preserved cauda epididymis  (4°C up to 12 days) for assisted reproductive technology. The semen was collected by artificial vagina, with the sperm motility, live sperm, acrosomal intact, and intact plasma membrane observed. Sperm motility was 75%, while for the live sperm, intact plasma membrane and sperm abnormality were 91.5%, 90.0%, and 1.8%, respectively. In the other study, sperm was collected from cauda epididymis by aspiration method and diluted in different media: 1) Brackett Oliphant (BO) media and 2) modified Phosphate Buffer Saline (mPBS). Evaluation of  sperm motility and intact plasma membrane were conducted after washing, counting and dilution of the sperm. The results of this study showed that the sperm motility and intact plasma membrane could be maintained better in BO rather than PBS medium although they were not statistically different (P>0.05). At day 12 of preservation, the motility and intact plasma membrane of sperm collected from cauda epididymis were 0.7% and 1.33% for motility and plasma membrane intact, respectively. These findings showed that the Garut rams semen was qualified for AI and frozen processing; in vitro embryo production by introducing the assisted reproductive technology such as intracytoplasmic sperm injection (ICSI) could be applied by using the sperm collected from preserved cauda epididymis until 12 days of preservation at 4°C. Keywords :   Reproduction/spcrmatogenesis/inscmination/Garut rams/small ruminant
OPTIMIZATION OF CELLULASE PRODUCTION WITH PENICILLIUM NALGIOVENSE Sll GROWN ON PRETREATED WHEAT POLLARD TRESNAWATI PURWADARIA; AGNES T. KUMALASARI; TUTIHARYATI; PIUS P. KETAREN; ARNOLD P. SINURAT1
BIOTROPIA - The Southeast Asian Journal of Tropical Biology No. 23 (2004)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (360.752 KB) | DOI: 10.11598/btb.2004.0.23.202

Abstract

The cellulase production with Penicillium nalgiovense S11 on wheat pollard was enhanced using substrate pretreatments, i.e.: (i) mechanic process by Wiley milling, (ii) reducing sugars removal by water soaking, and (iii) chemical pretrcatment by 0.5% NaOH soaking at 100°C. The enzyme production stated as enzyme activities of all prctrcated substrates were higher than the untreated substrate. Although soaking with water showed significant increase in enzyme activities, the highest CMCase (EC 3.2.1.4), FPase (filter papcrase) and p-glucosidase (EC 3.2.1.21) were observed on NaOH pretreated pollard. The NaOH prctreatment also enhanced the enzyme production by increasing substrate concentration from 2 to 4%. The optimal incubation time in the cellulase production on 4% NaOH-pretrcated pollard was observed on the fifth day. Addition of 250 ppm glucose also increased the enzyme activities. The optimal treatments increased the specific activities of CMCase, FPase, and |3-glucosidase into 60, 4, and 198  times, respectively, as compared to the specific activities on 2% unpretreated pollard. Keywords  :   Cellulase production/ pollard pretreatments/ water soaking/ NaOH  soaking
FISHERIES ASSOCIATED WITH MANGROVE ECOSYSTEM IN INDONESIA: A View from a Mangrove Ecologist SUKRISTIJONO SUKARDJO
BIOTROPIA - The Southeast Asian Journal of Tropical Biology No. 23 (2004)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2004.0.23.201

Abstract

Blessed with mangrove area of some 9.6 million ha in extent, Indonesia represents an important country with fishery resources being a source of food and nutrients. The fishery resources utilized by man, such as fishes, crustaceans and mollusks that are found in the mangrove ecosystem/swamp area arc enormous. There is a range of species caught in the mangrove and surrounding areas with over 70 species. However, commercially valued species are limited to a few such as rabbit fish, snapper, grouper, marline catfish, fringe-scale sardine, and anchovy. Leaf detritus from mangroves contribute a major energy input into fisheries. But information about the study on the relationship between fishery species and mangroves, ecologically and biologically, arc scanty. The mangrove is a physiographic unit, the principal components of which arc organisms. Therefore, the problems are predominantly of a biological nature (e.g., mangroves - fishery relationship). Positive correlation between the mangrove area and penaeid shrimp catch found in Indonesia, the Philippines, Australia and Mexico. Finally, the most important part of the variance of the MSY (Maximum Sustainable Yield) of penaieds (53% of the variance) could be explained by a combination of area of mangrove habitats and latitude. Keywords  :   Indonesia/Mangrove/Ecosystem/Fisheries/Ecology/Coastal areas/Fishes/Molluscans/ Crustaceans.

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