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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Ilmu Ternak Veteriner
TRESNAWATI PURWADARIA
Indonesian Research Institute for Animal Production
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

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OPTIMIZATION OF CELLULASE PRODUCTION WITH PENICILLIUM NALGIOVENSE Sll GROWN ON PRETREATED WHEAT POLLARD TRESNAWATI PURWADARIA; AGNES T. KUMALASARI; TUTIHARYATI; PIUS P. KETAREN; ARNOLD P. SINURAT1
BIOTROPIA - The Southeast Asian Journal of Tropical Biology No. 23 (2004)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (360.752 KB) | DOI: 10.11598/btb.2004.0.23.202

Abstract

The cellulase production with Penicillium nalgiovense S11 on wheat pollard was enhanced using substrate pretreatments, i.e.: (i) mechanic process by Wiley milling, (ii) reducing sugars removal by water soaking, and (iii) chemical pretrcatment by 0.5% NaOH soaking at 100°C. The enzyme production stated as enzyme activities of all prctrcated substrates were higher than the untreated substrate. Although soaking with water showed significant increase in enzyme activities, the highest CMCase (EC 3.2.1.4), FPase (filter papcrase) and p-glucosidase (EC 3.2.1.21) were observed on NaOH pretreated pollard. The NaOH prctreatment also enhanced the enzyme production by increasing substrate concentration from 2 to 4%. The optimal incubation time in the cellulase production on 4% NaOH-pretrcated pollard was observed on the fifth day. Addition of 250 ppm glucose also increased the enzyme activities. The optimal treatments increased the specific activities of CMCase, FPase, and |3-glucosidase into 60, 4, and 198  times, respectively, as compared to the specific activities on 2% unpretreated pollard. Keywords  :   Cellulase production/ pollard pretreatments/ water soaking/ NaOH  soaking
Effectivity of Dry and Liquid BS4 Enzymes in Improving Performance of Broiler Chickens Fed Different Nutrient Density Diet Arnold Parlindungan Sinurat; Tuti Haryati; Nurul Pratiwi; Tresnawati Purwadaria
Jurnal Ilmu Ternak dan Veteriner Vol 27, No 2 (2022): June 2022
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v27i2.3051

Abstract

Supplementation of enzymes in feed is now commonly practiced to increase the nutrient availability of feed and the performance of poultry. A new enzyme called BS4 was produced by cultivating Eupenicilium javanicum. It is necessary to test the efficacy of this enzyme since the effectiveness of enzyme supplementation depends on many factors. An experiment was conducted to study the effect of dietary BS4 enzyme supplementation in improving the performance of broiler chickens. A number of 300 broilers DOC was distributed into 30 pens and reared until 35 d. Six experimental diets i.e., factorial of 2 (Standard diet, and low nutrient density diet) X 3 (Control, BS4 liquid enzyme, and BS4 powder enzyme) were formulated with 5 replications. The performance (feed intake, body weight, FCR, and survival rates) were observed during the starter (1-21 d) and whole (1-35 d) periods. At the end of the trial, measurements were also made on the carcass yield, abdominal fat, liver, and gizzard weights. Results showed that performances of broilers from 1-35 d were not significantly affected by interaction between nutrient density and enzyme supplement. The nutrient density also did not affect performances of broilers. However, dietary enzyme supplementation significantly reduced feed intake and improved FCR of broilers as compared to the control. Supplementation of BS4 in liquid or powder form, reduced feed intake by 3.6%. Supplementation of liquid and powder BS4 enzymes improved FCR by 6.4% and 8.9%, respectively, but no different effect between liquid and powder BS4 enzymes on performance of broilers. Nutrient density, enzyme supplementation, and interactions between the two factors did not significantly influence carcass yield, abdominal fat, liver, and gizzard relative weights of broilers.
Co-Authors AGNES T. KUMALASARI ARNOLD P. SINURAT1 Arnold Parlindungan Sinurat Nurul Pratiwi PIUS P. KETAREN Tuti Haryati TUTIHARYATI