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BIOTROPIA - The Southeast Asian Journal of Tropical Biology
Published by Southeast Asian Regional Centre for Tropical Biology
ISSN : 02156334     EISSN : 1907770X     DOI : http://dx.doi.org/10.11598/btb
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary
BIOTROPIA, The Southeast Asian Journal of Tropical Biology, is a scientific publication of the Southeast Asian Ministers of Education Organization (SEAMEO) – Southeast Asian Regional Center for Tropical Biology (BIOTROP). It publishes articles in the broad areas of tropical ecosystems and environmental impacts, biodiversity conservation and sustainable development and biotechnology.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 6 Documents
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Search results for , issue "Vol. 19 No. 1 (2012): BIOTROPIA Vol. 19 No. 1 June 2012" : 6 Documents clear
A SPECIES-SPECIFIC PCR ASSAY BASED ON THE INTERNAL TRANSCRIBED SPACER (ITS) REGIONS FOR IDENTIFICATION OF MYCOSPHAERELLA EUMUSAE, M. FIJIENSIS AND M. MUSICOLA ON BANANA Hidayat, Iman
BIOTROPIA Vol. 19 No. 1 (2012): BIOTROPIA Vol. 19 No. 1 June 2012
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (370.716 KB) | DOI: 10.11598/btb.2012.19.1.232

Abstract

A study on development of a rapid PCR-based detection method based on ITS region of M. eumusae, M. fijiensis, and M. musicola on banana was carried out. The main objecive of this study was to develop a fast and species-specific PCR-based detection method for the presence of Mycosphaerella species on banana. The methods include collection of specimens, morphological identification supported by molecular phylogenetic analysis, RFLP analysis, species-specific primers development, and validation. Two species of Mycosphaerella, namely, M. fijiensis and M. musicola, and one unidentified Pseudocercospora species were found in Java island. Three restriction enzymes used in the RFLP analysis, viz, AluI, HaeIII, and TaqI were capable to discriminate M. eumusae, M. fijiensis, and M. musicola. Two species-specific primer pairs, viz, MfijF/MfijR and MmusF/MmusR have been successfully developed to detect for the presence of M. fijiensis and M. musicola, respectively.
MOLECULAR IDENTIFICATION AND PHYLOGENETIC RELATIONSHIP AMONG LOCAL, SANGKURIANG, AND AFRICAN CATFISH BASED ON RAPD MARKER Nuryanto, Agus
BIOTROPIA Vol. 19 No. 1 (2012): BIOTROPIA Vol. 19 No. 1 June 2012
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13132.152 KB) | DOI: 10.11598/btb.2012.19.1.49

Abstract

Catfish species are commercially cultivated freshwater fish by fish farmer. Formerly, only one species of catfish had been cultivated, namely local catfish (walking catfish) Clarias batrachus.  However, since 1985, local catfish was replaced by a new commodity from Africa called African catfish (C. gariepinus).  In 2004, BBPBAT Sukabumi had introduced a new strain of catfish, namely Sangkuriang catfish.  Taxonomic analysis needed, especially for Sangkuriang catfish using molecular marker, in order to get a clearer picture about its genetics departure from their parental, so their taxonomic status and relationships with local and African catfish is clearer.  One of the molecular markers is randomly amplified polymorphic DNA (RAPD) markers. Species status defined based on the presence and absence of specific band on each catfish.  Phylogenetics relationships was inferred from phylogenetic tree which was built using UPGMA tool as implemented in NTSYS software based on the similarity of RAPD band pattern. The result showed that Sangkuriang and local catfish had specific RAPD markers, while African catfish was not. This means that all RAPD markers of African catfish were shared with those of two others catfish. Local catfish phylogenetically distantly related to African and Sakuriang catfish. Only one individual of African catfish (D4) genetically close related to Sangkuriang catfish while the rest individuals of African catfish genetically closed related to green catfish. This means that African catfish showed very divergence genetic constituent.    Key words: catfish, molecular identification, phylogenetic relationships, RAPD
Plant community and soil relationship following wildfires from nuees ardentes on Mt. Merapi Sutomo, Sutomo; Fardila, Dini
BIOTROPIA Vol. 19 No. 1 (2012): BIOTROPIA Vol. 19 No. 1 June 2012
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13052.702 KB) | DOI: 10.11598/btb.2012.19.1.248

Abstract

At a local scale, vegetation patterns are known to have strong relationships with edaphic factors. In the case of Mt. Merapi volcanic eruption, studies on the relationships between edaphic factors and plant community composition and distribution following the nuees ardentes-ignited wildfires will provide valuable information on post-disturbance secondary successional processes. We collected soil and vegetation data from five different ages of nuees ardentes fire-affected secondary succession forests and one un-affected forest. Our results showed significant correlation of species composition and edaphic factors among sites of secondary forests. Nitrogen and soil water content were found to be the important factors in structuring species composition in the youngest site, where the N-fixing legume species such as Calliandra callothyrsus was found to be dominant. CCA ordination also revealed strong negative correlation between nitrogen content and legume group, while non-legume group were positively correlated with other edaphic factors such as phosphor and potassium. These findings suggested that changes in soil properties due to recovery of this system after volcanic eruption correlated with plant community composition and can be crucial factors in driving the successional trajectory. Understanding ecosystem reassembly process and trajectory of succession will improve how we approach the restoration of Mt. Merapi ecosystem.
Invasive grass in Indonesia: What happen up to 2012? with comparison other institution and government sumadijaya, alex
BIOTROPIA Vol. 19 No. 1 (2012): BIOTROPIA Vol. 19 No. 1 June 2012
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (119.296 KB) | DOI: 10.11598/btb.2012.19.1.245

Abstract

p { margin-bottom: 0.08in; } Invasive grass with dominant presence in ecosystem is often a neglected flora component. Meanwhile, the term “invasive” must be standardized, for practical purpose must be considered as urgent matter. Various attempts to compile invasive grass in Indonesia were conducted sporadically by several experts across generations. Scattered data along pile of literatures awaiting to be written in order to produce a comprehensive list of invasive grass. This paper represents the first attempt in drafting the invasive grass list of Indonesia by using literatures.
DENGUE EARLY WARNING MODEL USING CLIMATE INFORMATION Hidayati, Rini -
BIOTROPIA Vol. 19 No. 1 (2012): BIOTROPIA Vol. 19 No. 1 June 2012
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2012.19.1.236

Abstract

The use of early warning system is potential adaptation options to reduce the impact of the climate variability and change.  This study aims to develop a dengue early warning model using climate information.  The model was developed through three steps of analysis.  First step was to determine the length of periods used in prediction and optimal time for eradicating Aedes aegypti mosquito’s breeding sites.  Second step was to identify the best prediction model of dengue incidence rate (IR).  Third step was to develop an early warning model using stochastic spreadsheet.   It was found that the best predictors for predicting dengue incidence rate at week-n (IRn) were (1) rainfall index with two weeks lead time (ICHn-2).  The rainfall index of week-nth is a function of three week moving averages rainfall (CH3), i.e. (CH3n-1.155*CH3n-1+0.702*CHn-2), and (2) IR with one week lead time (IRn-1).  The IR model prediction was IRn = 0.795*IRn-1+0.067*ICHn-2 with R2=76.6%.  These models (result model from first and third step) can be used to provide early warning on optimum time for controlling the mosquito’s breeding sites and the need for fogging action in order to avoid the dengue incidence rate beyond the critical limit defined by the Ministry of Health
SOIL WATER BALANCE, BIOMASS AND YIELD OF POTATO CROP (Solanum tuberosum L.) GROWN IN HIGH ALTITUDE HUMID TROPICS OF INDONESIA Salwati, R; Handoko, I
BIOTROPIA Vol. 19 No. 1 (2012): BIOTROPIA Vol. 19 No. 1 June 2012
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2012.19.1.82

Abstract

This research calculated water loss through actual evapotranspiration and runoff (ETa+Ro) of potato crop in high altitude of humid tropics in Indonesia by using water balance approach.  The treatments consist of two row spacings and three seed sizes for sowing.  Soil water contents of six combination of treatments were measured weekly to the soil depth of 100 cm to calculate water loss by actual evapotranspiration and runoff.  Water loss by ETa+Ro was much higher (average of 18.2 mm/day) compared to average potential evapotranspiration (7.5 mm/day).  Despite high rainfall during the growing season (1314 mm), actual evapotranspiration could reduce soil water content to 60 cm soil depth due to the high runoff caused by limited capacity of water infiltration into the soil.  Total ETa+Ro averaged for all treatments was 1365 mm which was comparable among treatments. Higher soil water content resulted in larger crop biomass and higher tuber yield.  On the other hand, larger seed size produced greater crop biomass and tuber yield irrespective of the variation of soil water contents in the treatments.

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