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JC-T (Journal Cis-Trans) : Jurnal Kimia dan Terapannya
Published by Universitas Negeri Malang
ISSN : 25496565     EISSN : 25496573     DOI : 10.17966
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
JC-T (Journal Cis-Trans) : jurnal kimia dan terapannya is published by Universitas Negeri Malang, Faculty of Mathematics and Science, Chemistry Department.
Arjuna Subject : Kimia(semua kategori) - Kimia (Lain-Lain)
Articles 6 Documents
 1 
Search results for , issue "vol. 1 no. 2 (2017)" : 6 Documents clear
Pemekatan Keratinase dari Bacillus sp. 24 untuk Meningkatkan Aktivitas Dehairing Suharti Suharti
JC-T (Journal Cis-Trans) : Jurnal Kimia dan Terapannya Vol. 1 No. 2 (2017)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um026v1i22017p001

Abstract

Keratinase dapat diproduksi oleh Bacillus sp MD24 dengan menggunakan bulu ayam sebagai substrat. Ekstrak kasar keratinase tersebut terbukti dapat digunakan sebagai agen dehairing. Namun demikian, proses dehairing memerlukan waktu 72 jam karena rendahnya aktivitas keratinase. Penelitian ini bertujuan meningkatkan kemampuan keratinase sebagai agen dehairing. Dua hal telah dilakukan dalam upaya meningkatkan aktivitas ektrak kasar enzim yaitu peningkatan produksi enzim dan pemekatan enzim. Usaha untuk meningkatkan produksi enzim dilakukan dengan suplementasi media menggunakan sumber karbon tambahan berupa gula sederhana (sukrosa, glukosa, atau fruktosa). Sedangkan pemekatan enzim dilakukan melalui pengendapan enzim menggunakan amonium sulfat dengan tingkat kejenuhan 80%. Enzim hasil pengendapan diuji kemampuannya dalam dehairing kulit kambing pada berbagai waktu inkubasi. Penelitian menunjukkan bahwa suplementasi media dengan karbon sederhana memberikan hasil yang kontradiksi dimana penambahan sukrosa, glukosa, atau fruktosa justru menurunkan produksi ekstrak kasar keratinase. Sedangkan pemekatan mampu meningkatkan aktvitas enzim dari 8,79 U/mL sebesar 20,29 U/mL dan mampu menghilangkan bulu dengan waktu perendaman selama 48 jam.
Kajian Pengaruh Waktu dan pH Optimum dalam Adsorpsi Methyl Violet dan Methylene Blue Menggunakan Abu Daun Bambu Kuntari Kuntari
JC-T (Journal Cis-Trans) : Jurnal Kimia dan Terapannya Vol. 1 No. 2 (2017)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um026v1i22017p014

Abstract

Adsorption of methyl violet and methylene blue has been done on using bamboo leaf ash. This researchaims to study the characterization adsorbent by FT-IR spectrophotometry and adsorption with the parametersinteraction time and pH. The concentration of the dye was determined quantitively by UV-Visspectrophotometer. FT-IR spectogram of bamboo leaf ash show that typical fingerprint in IR spectrogram ofaf asdeobent with Si-O and Al-O groups. The adsorption results showed that optimum methyl violet adsorptionat 12 minutes and pH 11 with adsorption capacity of 22.8738 mg/g and adsorption of optimum methylene blueat contact time of 8 minutes and pH 11 with adsorption capacity of 17.9989 mg/g
Aktivitas Antioksidan Ekstrak Biji Buah Areca vestiaria Giseke dan Fraksinya dengan Metode DPPH (2,2-diphenyl-1-picrylhydrazyl) Nur Candra Eka Setiawan; Hilda Amalia
JC-T (Journal Cis-Trans) : Jurnal Kimia dan Terapannya Vol. 1 No. 2 (2017)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um026v1i22017p009

Abstract

Antioxidant widely used to prevent degeneratif desease and anti aging. The seed of Pinang yaki fruitscontain some compounds which have antioxidant activity. The purposes of this research were to know the antioxidant activity of ethanolic extracts and fractions of the seed of pinang yaki fruits and the IC50 value of its antioxidant activity. The samples of the seed were prepared separately by soxhlet extraction method with ethanol 96% as the solvent. Then fractionation process used three different solvents are n-hexane, ethyl acetate, and ethanol-water. Futhermore, the calculation of yield, phytochemical screening, and test of antioxidant activity using DPPH method. Yield calculation result showed ethanolic extract, fraction of nhexane, fraction of ethyl acetate, fraction of ethanol-water 17,1%, 10,8%, 18,2%, 52,8%. Phytochemical screening of Flavonoids ethanolic extract, fraction of ethyl acetate, fraction of ethanol-water showed positive contains Flavonoid. IC50 value of ethanolic extract, fraction of n-hexane, fraction of ethyl acetate, fraction of ethanol-water 94,5 µg/mL, 445,7 µg/mL, 44,7 µg/mL, 91,4 µg/mL. The conclusion of this research is extract of ethanol and the fraction of seed of pinang yaki fruits has the antioxidant activity. Fraction of ethyl acetate has the higher antioxidant activity 44,7 µg/mL.
Sintesis dan Karakterisasi Senyawa Kompleks dari Kadmium nitrat dan 1,3-bis(difenilfosfino)propana dengan Stoikiometri 1 : 1 Wawan Setiawan; Fariati; Effendy
JC-T (Journal Cis-Trans) : Jurnal Kimia dan Terapannya Vol. 1 No. 2 (2017)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um026v1i22017p019

Abstract

Coordination compound of cadmium nitrate and dppp has not been reported. The purpose of this research are to synthesize coordination compoound of cadmium nitrate and dppp with 1:1 stoichiometry and determine its structure. The coordination compound obtained is colourless cry stalline solid having beam shape with melting point of 272-275̊C. Results of electrical conductivity and qualitativeC fkekfksnitrate ion tesindicates that the compound is a molecular one. EDX analysisshow the empirical formula C27H26CdN2O6P2. The accepted compound is [Cd(ONO2)2(dppp)] with distorted tetrahedral structure and average free bond energy -47.5 kJ/mol.
Penentuan Ketidakpastian Pengukuran Kadar Kafein pada Biji Kakao (Theobroma Cacao L.) Menggunakan Spektrofotometri UV-Vis Devita Wijiyanti; Thorikul Huda
JC-T (Journal Cis-Trans) : Jurnal Kimia dan Terapannya Vol. 1 No. 2 (2017)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um026v1i22017p022

Abstract

A study on the determination of uncertainty in measurement of caffeine content in cocoa beans by UV -Vis spectrophotometer method. The aim of this study was to determine the uncertainty of measurement of caffeine content in cocoa beans. Caffeine in cocoa bean extract was analyzed using UV-Vis spectrophotometer at 273 nm wavelength, obtained by linear regression equation y=0,0497x+0,0028 with determinant coefficient R2 0,9996. The results showed that caffeine content of 26.40 mg/g. LOD and LOQ values were 0.52 mg/L and 1.73 mg/L. The contributing factors of uncertainty are concentration (c), mass (m), dilution factor and precision with an estimated uncertainty of measurement of 3.11 mg/g. Test results can be reported with a value of 26.40 ± 3.11 mg/g.
Aktivitas Antifungi Ekstrak Metanol Kulit Batang Jambu Mete...25Aktivitas Antifungi Ekstrak Metanol Kulit Batang Jambu Mete terhadap Candida albicans Dahlisa Soleman; Nur Candra Eka Setiawan
JC-T (Journal Cis-Trans) : Jurnal Kimia dan Terapannya Vol. 1 No. 2 (2017)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um026v1i22017p025

Abstract

Candida albicans is a fungus that commonly cause infections in humans where around 70% of the fungal infections caused by Candida albicans. The purpose of this research was to know the activity of the methanol extracts of antifungi cashew bark (Anacardium occidentale L.) againts Candida albicans with agar well difussion method. This research includes experimental research conducted in the Microbology laboratory at Academy of Pharmacy Putra Indonesia Malang. Results of the study showed extracts of bark cashew nuts with a concentration of 50%, 75%, and 100% can inhibit the growth of Candida albicans with the drag of zone 25,997 mm, 28,334 mm, and 29,995 mm. From this research it can be concluded that the extract of bark cashew nuts have antifungi activity against Candida albicans. Further research is needed to find out the effectiveness of the methanol extract of bark of cashew nuts in inhibiting the growth of Candida albicans starts from the lowest concentration.

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