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Brigitta Laksmi Paramita
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Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia
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Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Published by Universitas Atma Jaya Yogyakarta
ISSN : 25273221     EISSN : 2527323X     DOI : doi.org/10.24002/biota
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN 0853-8670. Biota terbit tiga nomor dalam satu tahun (Februari, Juni, dan Oktober).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 22 Documents
 1   2   3 
Search results for , issue "Vol 9, No 2 (2004): June 2004" : 22 Documents clear
Potensi Mangrove Sebagai Tanaman Obat (Short Communication) Purnobasuki, Hery
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2004): June 2004
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i2.2901

Abstract

Tumbuhan mangrove di Indonesia merupakan yang terbanyak di dunia, baik dari segi kuantitas area (+ 42.550 km2) maupun jumlah species (+ 45 species) (Spalding et al. 2001). Mangrove mempunyai banyak sekali manfaat yang bersinggungan langsung dengan kehidupan manusia di daratan, mulai dari manfaat ekologi sampai dengan sebagai sumber pangan dan obat.
Konstruksi Vektor Biner Mengandung Gen Hibrid cryIB-cryIAa untuk Transformasi Agrobacterium Tanaman Padi (Oryza sativa L) Syamsidah Rahmawati; Inez H. Slamet-Loedin
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2004): June 2004
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i2.2892

Abstract

This study aimed to obtain binary vectors carrying  cryIB-cryIAa hybrid gene suitable for Agrobacterium-mediated transformation of two Indonesian rice cultivars, Rajalele (Javanica) and Cisadane (Indica).  Introduction of hybrid Bt toxin, encoded by cryIB-cryIAa hybrid gene with different binding receptor in the insect gut driven by maize ubiquitin promoter, was intended to reduce the possibility of the appearance of stem borer resistant to Bt toxin. A HindIII fragment containing the ubi cryIB-cryIAa hybrid gene was excised from pBKS ubi cryIB-cryIAa and cloned into the HindIII site of binary vector pCAMBIA 1301, 1303, or 1304.  Restriction analysis of recombinant plasmids confirmed the insertion of the gene and demonstrated that the gene inserted in two orientations. The recombinant plasmids were transformed into electrocompetent Agrobacterium tumefaciens EHA105 by electroporation.  Embryogenic calli of Rajalele and Cisadane were co-cultivated with A. tumefaciens EHA 105 harbouring binary vector pCAMBIA 1301, 1303, or 1304 containing ubi cryIB-cryIAa. GUS histochemical assay revealed that high transformation efficiency (75-100%) on both cultivars were obtained from embryogenic calli transformed with pCAMBIA 1303 or 1304.
Kajian Indeks Hematologis pada Empat Subpopulasi Gelatik Jawa (Padda oryzivora L.) Sang Putu Surata
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2004): June 2004
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i2.2893

Abstract

Gelatik Jawa (Padda oryzivora L) merupakan salah satu spesies burung yang terancam punah dan perlu dikonservasi. Salah satu penyebab kepunahan adalah ketidakmampuan spesies tersebut untuk beradaptasi dengan perubahan alam sekitarnya yang dapat ditunjukkan pada perubahan  ciri biokimiawi individu seperti indeks hematologis. Penelitian ini bertujuan untuk mempelajari apakah ada perbedaan indeks hematologis pada empat subpopulasi gelatik Jawa   di Jawa Barat, Jawa Timur, Bali Utara dan Bali Selatan.  Hasil penelitian menunjukkan bahwa indeks hematologis  darah  gelatik Jawa dari empat subpopulasi  tersebut dengan bobot badan  antara 21-30 g, memiliki  nilai rerata eritrosit 5,00±0,60 x 106/mm3; leukosit 2,35±0,86 x 103/mm3; PCV 61,32±11,62%; limfosit 58,25±17,32%; heterofil 35,93±15,72%; monosit 3,05±2,98%; dan basofil 0,05±0,21%.  Ratio heterofil terhadap limfosit (H/L) burung tidak meningkat seiring dengan meningkatnya suhu lingkungan. Diduga hal tersebut disebabkan gelatik Jawa sudah beradaptasi dalam waktu yang relatif lama terhadap lingkungan lokal.
Sebaran Tumbuhan Bawah Bekas Injakan Banteng (Bos javanicus d’Alton), Kerbau Liar (Bubalus bubalis), dan Rusa (Cervus timorensis) di Taman Nasional Baluran Suhadi Suhadi; Hadi S. Alikodra
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2004): June 2004
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i2.2894

Abstract

National Park is a nature conservation area has a wild  ecosystem and managed by zone system. Baluran National Park (BNP) is one of conservation area in Indonesia covered 25,000 hectare consists of 420 hectare of Bekol’s savanna and 150 hectare of Bama’s savanna. From  4.345 grass-feeding mammal  in BNP, it has estimated that their total weight about  777,000 kg  and  the total feeding need  about 77,700 kg  per day. On the other hand, the biomass of the grasslands only 86.125 kg/ha/day, and both Savannas could only produce biomass about 40,478.75 kg  per day which was  not enough to support the daily animal feeding. Decreasing of the biomass in BNP was assumed as a result of habitat destruction by animal step printings.  The purpose of  this study is to know the effect of animal step printing to the biomass production.  Samples was taking from April to December 1999 using quadrates method which was applied following the animal step printing and the control (non-stepped area). The plant samples were kept in plastic pocket and then identified  in the laboratory. The total  number of quadrates could be detected in this study were 150.  From this study it was concluded that  Grassland which was stepped by banteng, water buffalo and deer  decreased the number of plant species  until 38.88%, 27.27% and 18.18% respectively.  
Pola Pertumbuhan dan Produksi -Amilase Bacillus amyloliquefaciens pada Substrat Pati Jagung dengan Variasi pH Awal Media dan Waktu Inkubasi Sisilia Sri Wahyuningsih; Exsyupransia Mursyanti; P. Kianto Atmodjo
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2004): June 2004
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i2.2895

Abstract

The aims of this study  were to identify the growth curve of  B. amyloliquefaciens on  corn-starch and non corn starch addition media,  number of cells  and production of a-amylase on variety initial pH during the stationary phase. The growth curve of B. amyloliquefaciens was made using the water optical density on both  medium which has inoculated by microbes. The experimental design for the a-amylase production was factorial completely randomized design (6 x 3 x 3). There were two factors included in this study i.e. initial  pH of the media ( 5,  5.5,  6,  6.5,  7 and 7.5) and incubation times (16, 18 and 20 hours). The results showed that B. amyloliquefaciens growth curve on medium with corn starch was slower than on medium without corn starch. Production of  a-amylase and number of cells were having similar patterns in all treatments, i.e. increased until optimum pH and incubation time were reached. The number of cells and a-amylase production were optimal at pH 6.5 for 18 hours incubation whereas the number of cells  (about 2.8542 x 108 cells/ml)  and a-amylase production (1.4467 units/ml) were optimal at pH 6.5 for 18 hours  incubation.
Komposisi Jenis Ikan di Perairan Pantai Teluk Saleh, Nusa Tenggara Barat (NTB) dan Tanimbar Utara, Maluku Tenggara Langkosono Langkosono
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2004): June 2004
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i2.2896

Abstract

Study of the fish diversity in Saleh Bay, West Nusa Tenggara (NTB) has been carried out in August 2000, and in North Tanimbar, Maluku Tenggara   during November to  December 2002. The fishes  collecting in Saleh Bay using gillnet, beach seine, angling, and light fishing while in  North Tanimbar by beach seine. A total specimens which collected from Saleh Bay were 576 samples, consist of 108 species from 29 families while in North Tanimbar were found  2 763 specimens, representing 61 species from 28 families.     
Distribusi Southeast Asia Ovalocytosis (SAO) pada Berbagai Populasi Masyarakat di Kepulauan Sunda Kecil Fransiska Lanni
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2004): June 2004
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i2.2897

Abstract

The presence of some genetic markers related to malaria as hemoglobinopathies, enzymophaties and ovalocytosis in populations of Lesser Sunda Islands  are presumed to be quite high, since this archipelago is mostly malaria endemic. To elucidate the problem, a series of observations had been done among  579 blood samples which were collected from five different populations (Balinese, Sasak, Sumbawanese, Sumbanese,  and Alorese) representing inhibitant of Lesser Sunda Islands. In this paper we present the distribution of Southeast Asia Ovalocytosis (SAO) which was detected  by microscopic examination of the red blood cell morphology. Among these, 38 subjects were suspected as SAO whereas the incidence ranges from 2.2% in Balinese to 15.7% in Alorese.  It was predicted that the high incidence of SAO in Alorese might be associated with malaria selection to compensate the absence of hemoglobinophaties in this population.
Isolasi Bakteri Pengakumulasi Poliposfat I Made Sudiana
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2004): June 2004
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i2.2898

Abstract

Polyphosphate accumulating bacteria play  important key role on biological phosphorous removal. Activated sludge originated from wastewater treatment plant for drinking water production acclimated with anaerobic-aerobic phase in sequential batch reactor continuously succeed to establish microbial community that are able to adsorb glucose and acetate under anaerobic condition.  Acinetobacter calcoaceticus S1 was an isolated bacterium from community performing organic substrate absorption. S1 is able to adsorb glucose and acetate under anaerobic condition. During substrate absorption an increase of orthophosphate in bulk solution indicating phosphate released out of cell. Reduction of glycogen and polyhydroxybutirate (PHB) took place under anaerobic phase. S1 is a community member of anaerobic-aerobic system for removal of phosphorous in influent wastewater.
Kajian Kehadiran Inang Primer pada Pertumbuhan Semai Cendana Albertus Husein Wawo
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2004): June 2004
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i2.2899

Abstract

Sandalwood  (Santalum album L.)  is known as a fancy plant. Since they have  high economic value, it often over exploited.  As a consequence, the population  dramatically decreased  in their habitats.  Some efforts  have been  done  to conserve  this plant  in order to prevent their extinction, i.e.  seedling multiplication.  As a  hemiparasitic plant,  sandalwoods  need other  plants for a host which grow  around.  Therefore, determining of the the primary host is a necessary aspect in multiplication  of  sandalwood seedling.  This study  used  three  species  plants  to serve sandalwood seedling   as  primary host  in pot cultures consist of local leucaena (Leucaena glauca), vilosa (Acacia villosa)   and calliandra  (Calliandra  calothyrsus).   The results  of this study indicated   that A. vilosa  is better for a primary  host  than  L. glauca and  C. calothyrsus as well. Number of  root connection  between  sandalwood seedlings   and their hosts  have a close correlationship   to  the leaf  number  and the  sandalwood seedling   dry weight, whereas no significant correlationshifp to their height.   
Uji Antagonis Trichoderma sp dan Gliocladium sp terhadap Fusarium Penyebab Penyakit Layu pada Beberapa Jenis Tanaman Pisang di Kebun Raya Purwodadi secara in-vitro Suharjono Suharjono; Tri Handayani Kurniati; Soejono Soejono; Susanti Dewi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2004): June 2004
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i2.2900

Abstract

Purwodadi Botanical Garden has more than one hundred collection of cultivated bananas. The major problem in keeping their existency is  the occuring of  Fusarium wilt disease  which was commonly controlled by pesticide or eradication to terminate the infectious cycles. Trichoderma and Gliocladium were frequently used as antagonist fungus in controlling the Fusarium wilt disease in kenaf, tomato or  the other plants. So,  this study aims was to know the  potency of Trichoderma and Gliocladium on Fusarium growth inhibition. The experimental design was done by Randomized Complete Design Factorial using three factors i.e. antagonist fungus, Fusarium and growth distance. The level of retardation was measured by the growth distance of Fusarium  that interacted to antagonist fungus and data analysis were conducted  by Analysis of Variance (ANOVA). The results showed that Trichoderma has higher inhibition than Gliocladium and the highest inhibition occurred at 1 cm  distance of inoculum which was performed on Fusarium 4 (77.78%). There was not any significant differencies between Fusarium 1 with Trichoderma (73.55%) and Fusarium 2 with Gliocladium (73.33%). At 2 cm distance, the highest inhibition occurred in Fusarium 3 by Trichoderma (72.71%), which  was not significantly different with Fusarium 1, 2 and 4. While at 3 cm distance, the highest inhibition on Fusarium 4 by Trichoderma  was 51.11% and not significantly different from Fusarium 1, 2 and 3.

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