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All Journal HAYATI Journal of Biosciences Jurnal Bios Logos BIOTROPIA - The Southeast Asian Journal of Tropical Biology IPTEK Journal of Proceedings Series Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Pelita Perkebunan Natural B Planta Tropika Journal of Tropical Biodiversity and Biotechnology Biosaintifika: Journal of Biology & Biology Education Jurnal Purifikasi Narra J Berkala Penelitian Hayati Jurnal Kreativitas dan Inovasi (Jurnal Kreanova) Borneo Journal of Pharmacy Biosaintifika: Journal of Biology & Biology Education Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Hery Purnobasuki
Departement Of Biology, Faculty Of Science And Technology, Universitas Airlangga.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Veterinary

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Hairy Root Induction on Justicia gendarussa by Various Density of Agrobacterium rhizogenes strain LB 510 Wahyuni, Dwi Kusuma; Hafida, Siti Nur; Ermayanti, Tri Muji; Wardoyo, Bambang Prajoga Eko; Purnobasuki, Hery; Utami, Edy Setiti Wida
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.8223

Abstract

Gandarusa (Justicia gendarussa Burm.f.) is an Indonesian medicinal plant that has many benefits as drug and male contracetive. For industrial needs, Gandarusa must be available in large quantity. Hairy root culture is one of methode to produce phytochemistry compound. The objective of the study was to examine the effect of various density of Agrobacterium rhizogenes strain LB510 on hairy roots induction of gandarusa (Justicia gendarussa Burm.f.) leaf plant. Leaf explants were inoculated in MS liquid medium with various density of OD600 = 0.1; 0.2; 0.3; 0.4; and 0.5. Explants were co-cultivated for 2 days on MS solid medium without any hormone then sub-cultured on MS solid medium containing antibiotic cefotaxim 300 ppm, in dark condition. The data were analyzed descriptively and statistically. The results showed that various density of Agrobacterium rhizogenes strain LB510 was affected the lenght of hairy roots induction of J. gendarussa Burm.f., but these was not effected toward lenght formation time and number of hairy root. The treatment of OD600 0.2 was the best treatment for hairy root induction on Justicia gendarussa Burm. f. This data could be used for optimized the quality of methode of hairy root induction.
Struktur Lentisel pada Pneumatofor Avicennia marina: sebagai Alat Pengantar Oksigen Pada Akar Mangrove Purnobasuki , Hery
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 2 (2011): June 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (355.561 KB) | DOI: 10.24002/biota.v16i2.113

Abstract

Telah dilakukan penelitian terhadap lentisel pneumatofor Avicennia marina menggunakan mikroskop cahaya dan mikroskop pemindai electron untuk mengetahui keterkaitan perkembangan dan struktur dari fungsi lentisel sebagai alat pengantar oksigen pada akar mangrove. Hasil penelitian menunjukkan bahwa struktur lentisel bervariasi dalam hal ukuran dan morfologi, mulai dari bentuk seperti kawah dengan jaringan lunak di bagian tengahnya. Lentisel tersusun dari jaringan pelengkap yang tersusun atas sel-sel berdinding tipis dan membentuk ruang-ruang interseluler. Sel-sel tersebut mengandung lignin yang terdeteksi positif dengan pewarnaan sfranin. Dalam pertumbuhannya, sel-sel pelengkap merusak lapisan peridermis dan selanjutnya lentisel berfungsi sebagai bagian dari sistem saluran penghantaran udara.
Ammonia Removal Using Coconut Shell Based Adsorbent: Effect of Carbonization Duration and Contact Time Oktavitri, Nur Indradewi; Purnobasuki, Hery; Kuncoro, Eko Prasetyo; Purnamasari, Indah
IPTEK Journal of Proceedings Series No 4 (2017): 2nd International Seminar on Chemistry 2016
Publisher : Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.226 KB) | DOI: 10.12962/j23546026.y2017i4.3072

Abstract

The preparation of adsorbent using activated coconut shell was investigated at carbonization duration and adsorption contact time.  The coconut shell was activated using physical and chemical activation.  This research focused on variation of physical activation such as carbonization. The carbonization had two steps, there are high temperature and medium temperature. This research used 500oC at high temperature and 220oC at medium temperature. The duration of medium temperature was varied in 1, 2, and 4 hours. The chemical activation used H3PO4. The ammonia concentration of simulated wastewater around 6000 mg/l. The acclimatization period was 8 d. The coconut shell ability for ammonia removal was observed after acclimatization period in 0, 2, 4, 6, and 8d.  The effect of carbonization period and contact time on ammonia adsorption of dairy simulated wastewater was discussed in this research. This research used anaerobic digestion with 10 liter of volume. The concentration of ammonia was measured with nessler methods. Carbonization duration and contact time increased the adsorption capacity. From the obtained result of adsorption isotherm, the Langmuir equation was the best fit for all carbonization period, especially 4-hour carbonization period.
Potensi Mangrove Sebagai Tanaman Obat (Short Communication) Purnobasuki, Hery
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2004): June 2004
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i2.2901

Abstract

Tumbuhan mangrove di Indonesia merupakan yang terbanyak di dunia, baik dari segi kuantitas area (+ 42.550 km2) maupun jumlah species (+ 45 species) (Spalding et al. 2001). Mangrove mempunyai banyak sekali manfaat yang bersinggungan langsung dengan kehidupan manusia di daratan, mulai dari manfaat ekologi sampai dengan sebagai sumber pangan dan obat.
Effects of Plant Media And Fertilization on The Growth of Orchid Plant (dendrobium sylvanum rchb. F.) in Acclimatization Phase Hariyanto, Sucipto; Jamil, Ahmad Ridlwan; Purnobasuki, Hery
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 7, No 1 (2019)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.2019.095.66-72

Abstract

This study was to evaluate the effect of plant media variation, time of fertilization, and combination of both on the growth of orchid plant Dendrobium sylvanum Rchb. f. in acclimatization phase. This study was designed using 6x4 factorial experimentation. The first factor was the variation of plant media: charcoals, coconut husk, bagasse, mixing of charcoal and coconut husk, combination of charcoal and bagasse, combination of coconut husk and bagasse. The second factor was fertilization time: no fertilization, 7 day-fertilization, 14 day-fertilization, and 20 day-fertilization for three months. The data were analysed by using Two Way MANOVA (α = 5%) and Duncan test. Based on the results, there were differences between media variation and fertilization time on the increase of leaf length and width, stem diameter, and root length of Dendrobium orchid in acclimatization phase. Combination of bagasse and coconut husk media showed the best result on media treatment. The best of fertilization time was 14 days. Moreover, the best of interaction between media and fertilization time showed by the combination of bagasse and coconut husk with 14 days-fertilization.
Induksi Akar Rambut Gandarusa (Justicia gendarussa Burm. f.) dengan Perlakuan Perbedaan Lama Waktu Infeksi Agrobacterium rhizogenes strain YM072001 dan A4T (The Induction of Gandarusa (Justicia gendarussa Burm. f.) Hairy Root under Different Infection Wahyuni, Dwi Kusuma; Nisa, Qonitatun; Purnobasuki, Hery; Ermayanti, Tri Muji; Prajoga, Bambang; Utami, Edy SW
JURNAL BIOS LOGOS Vol 5, No 2 (2015): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.5.2.2015.10548

Abstract

Abstrak Penelitian ini bertujuan untuk menginduksi akar rambut eksplan daun Gandarusa (Justicia gendarussa Burm.f.) dengan perlakuan perbedaan lama waktu infeksi Agrobacterium rhizogenes strain YM072001 dan A4T dan mengevaluasi lama waktu infeksi terbaik untuk induksi akar rambut. Eksplan daun diinokulasi di media MS (Murashige dan Skoog) cair yang berisi bakteri Agrobacterium rhizogenes dengan OD600 = 0,1 selama 10, 20, 30, 40, 50, dan 60 menit. Kokultivasi di MS padat selama 2 hari, lalu disubkultur ke media MS padat. Data dianalisis secara deskriptif. Akar rambut mulai tumbuh pada minggu ketiga. Induksi akar rambut berhasil pada eksplan dengan perlakuan strain YMB072001 dengan lama waktu infeksi 20, 40, dan 50 menit. Strain A4T juga berhasil menginduksi eksplan membentuk akar rambut dengan perlakuan 10 menit. Lama waktu infeksi terbaik untuk strain YMB072001 adalah 20 menit dan untuk strain A4T adalah 10 menit. Kata kunci: Agrobacterium rhizogenes, akar rambut, Justicia gendarussa Burm. f., waktu infeksi   Abstract The objective of this study were to induce hairy root of Gandarusa leaf explants by differences of Agrobacterium rhizogenes infection time treatment and to evaluate the best infection time for induction. Leaf explants were inoculated on MS (Murashige dan Skoog) liquid medium with bacterial concentrations of OD600 = 0,1 for 10, 20, 30, 40, 50, and 60 minutes and 2 days co-cultivated on MS0 solid medium then sub cultured on MS0 solid medium. Data were analyzed descriptively. Hairy roots were growing on the third week. Explants were successfully induced by strain YMB 072001 at 20, 40, and 50 minutes treatment. A4T strain was successfully induced by 10 minute of treatment. The best infection time for hairy root induction in gandarusa leaf explants for YMB 072001 strains was 20 minutes and for A4T strain was 10 minutes. Keywords: Agrobacterium rhizogenes, hairy root, infection time, Justicia gendarussa Burm. f.
Morphological Variation Interest on Several Varieties of Chrysanthemum morifolium Ramat Hery Purnobasuki; Anika Sindhya Dewi; Dwi Kusuma Wahyuni
Natural B, Journal of Health and Environmental Sciences Vol 2, No 3 (2014)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1002.058 KB) | DOI: 10.21776/ub.natural-b.2014.002.03.2

Abstract

The aim of this research was to know the morphological variety of flower in Chrysanthemum morifolium Ramat through visual observation which use digital camera and then was analyzed descriptively. Varieties of Chrysanthemum morifolium Ramat in this research were C. morifolium var reagen pink, C. morifolium var puma purple, C. morifolium var evergreen, C. morifolium var boris becker, C. morifolium var stroika, C. morifolium var tiger, C. morifolium var remix purple, C. morifolium var jaguar red, C. morifolium var rhino white, C. morifolium var pasopati, C. morifolium var towntalk. From those 11 varieties showed the morphological differences of the flowers, which are the color of ray floret, the quantity of ray floret, the quantity of ray floret layers, the shape of ray floret, the tip of ray floret, the quantity of disk floret, the position of disk floret, the diameter of flower, the length of ray floret, the width of ray floret, the diameter of involucrum and the disk shape. However, this research also look the similarities between the varieties, which are the edge of ray floret (flat), the color of involucrum is green, the adhering of inter-involucrum (star like), the tip of involucrum (dull), transparent coating involucrum, the quantity of involucrum layers (2 layers), the color of the corolla tubes(yellow), the branched and yellow pistil, and yellow anther. From the results it can be concluded that Chrysanthemum morifolium Ramat has multiple the morphological differences and similarities of the flower morphology between the varieties.
Struktur Lentisel pada Pneumatofor Avicennia marina: sebagai Alat Pengantar Oksigen Pada Akar Mangrove Hery Purnobasuki
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 2 (2011): June 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v16i2.113

Abstract

Telah dilakukan penelitian terhadap lentisel pneumatofor Avicennia marina menggunakan mikroskop cahaya dan mikroskop pemindai electron untuk mengetahui keterkaitan perkembangan dan struktur dari fungsi lentisel sebagai alat pengantar oksigen pada akar mangrove. Hasil penelitian menunjukkan bahwa struktur lentisel bervariasi dalam hal ukuran dan morfologi, mulai dari bentuk seperti kawah dengan jaringan lunak di bagian tengahnya. Lentisel tersusun dari jaringan pelengkap yang tersusun atas sel-sel berdinding tipis dan membentuk ruang-ruang interseluler. Sel-sel tersebut mengandung lignin yang terdeteksi positif dengan pewarnaan sfranin. Dalam pertumbuhannya, sel-sel pelengkap merusak lapisan peridermis dan selanjutnya lentisel berfungsi sebagai bagian dari sistem saluran penghantaran udara.
Potensi Mangrove Sebagai Tanaman Obat (Short Communication) Hery Purnobasuki
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2004): June 2004
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i2.2901

Abstract

Tumbuhan mangrove di Indonesia merupakan yang terbanyak di dunia, baik dari segi kuantitas area (+ 42.550 km2) maupun jumlah species (+ 45 species) (Spalding et al. 2001). Mangrove mempunyai banyak sekali manfaat yang bersinggungan langsung dengan kehidupan manusia di daratan, mulai dari manfaat ekologi sampai dengan sebagai sumber pangan dan obat.
Effects of Plant Media And Fertilization on The Growth of Orchid Plant (dendrobium sylvanum rchb. F.) in Acclimatization Phase Sucipto Hariyanto; Ahmad Ridlwan Jamil; Hery Purnobasuki
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 7, No 1 (2019)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.2019.095.66-72

Abstract

This study was to evaluate the effect of plant media variation, time of fertilization, and combination of both on the growth of orchid plant Dendrobium sylvanum Rchb. f. in acclimatization phase. This study was designed using 6x4 factorial experimentation. The first factor was the variation of plant media: charcoals, coconut husk, bagasse, mixing of charcoal and coconut husk, combination of charcoal and bagasse, combination of coconut husk and bagasse. The second factor was fertilization time: no fertilization, 7 day-fertilization, 14 day-fertilization, and 20 day-fertilization for three months. The data were analysed by using Two Way MANOVA (α = 5%) and Duncan test. Based on the results, there were differences between media variation and fertilization time on the increase of leaf length and width, stem diameter, and root length of Dendrobium orchid in acclimatization phase. Combination of bagasse and coconut husk media showed the best result on media treatment. The best of fertilization time was 14 days. Moreover, the best of interaction between media and fertilization time showed by the combination of bagasse and coconut husk with 14 days-fertilization.
Co-Authors Adi Setyo Purnomo Adi Sofyan Ansori, Muhammad Agustina, Niken Ahmad Ridlwan Jamil Aini, Nur Sofiatul Alfian, Fariz R. Ali Agus Alifiansyah, Mochamad RT. Andi Gita Maulidyah Indraswari Suhri Anggraini, Dhea Anika Sindhya Dewi Ansori, Arif NM. Arya Zulfikar P. Aulia, Halimatus S. B. Saadah Bambang Prajoga Bambang Prajoga Eko Wardoyo Bambang Prajoga Eko Wardoyo, Bambang Prajoga Eko Bezhinar, Tatyana Bonkalo, Tatyana Burkov, Pavel Derkho, Marina Dewangga, Rezha Tanu Dini Ermavitalini Dwi Kusuma Wahyuni Dwika Bramasta Edy Setiti Wida Utami Edy SW Utami, Edy SW Eko Prasetyo K. Eko Prasetyo Kuncoro Elfianah, Verah Farid Kamal Muzaki Fetty, Amelia JT. Fitria Ardiyani Gudz, Petr Hamidah . Herdiansyah, Mochammad A. Herdiansyah, Mochammad Aqilah Hermawan, Endang Husna, Etika Ziadana Al IBNUL QAYIM Ida Kinasih Ika Sukma Dewi Indah Purnamasari Jakhmola, Vikash Jamil, Ahmad Ridlwan Junairiah Junairiah Khairullah, Aswin Rafif Kharisma, Viol D. Kharisma, Viol Dhea Kovalchuk, Dmitriy F. Leonardo Pascalis Dimas Cahyo Baskoro Maksimiuk, Nikolai Mawardani, Alfiyyana Nurrahma Mega Rosita Munawir Sazali Murtadlo, Ahmad Affan Ali Ni'matuzahroh Ni'matuzahroh Ni'matuzahroh Nina Ratna Djuita Ni’matuzahroh Ni’matuzahroh Nur Indradewi O Nur Indradewi Oktavitri Pratiwi, Intan A. Purnamasari, Indah Putra, Justitia ERP. Putra, Ramadhani Qonitatun Nisa, Qonitatun R.C. Hidayat Soesilohadi Rahadian Zainul Rahayu , Anisa Esti Rahmahana, Ratna Syifa'a Rahmahana, Ratna Syifa’a Ramadhani Eka Putra RC Hidayat Soesilohadi Rebezov, Maksim RIKA RAFFIUDIN Rizaldy, Rafli Rizam, Bilqis Naura Safira Rofiatun Solekha Rollando, Rollando Santini Ika Rafsanjani Setianingsih, Primanita NM. Shmeleva, Svetlana Sih Kahono Siska Yulia Rukhmana Siti Nur Hafida Siti Nur Hafida, Siti Nur Sucipto Hariyanto Teguh Hari Sucipto, Teguh Hari Thalita Meidina Tri Muji Ermayanti Tri Muji Ermayanti Tri Nurhariyati Triono Bagus Saputro, Triono Bagus Widyananda, Muhammad Hermawan