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INDONESIA
Jurnal Biotek Medisiana Indonesia
Published by Badan Penelitian dan Pengembangan Kesehatan, Kementerian Kesehatan
ISSN : 23015810     EISSN : 23548800     DOI : -
Core Subject : Health,
Health Professions
Jurnal Biotek Medisiana Indonesia published 2 times a year. This journal is a medium of information and research results and development areas for non-communicable diseases and public health program managers, as well as a means of communication the researchers /enthusiasts in the field of non-communicable diseases and infectious.
Arjuna Subject : -
Articles 5 Documents
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Search results for , issue " Vol 2, No 1 (2013)" : 5 Documents clear
Produksi Parthenogenetik Blastosis Mencit Sebagai Sumber Stem Cell Rinendyaputri, Ratih; Nikmah, Uly Alfi
Jurnal Biotek Medisiana Indonesia Vol 2, No 1 (2013)
Publisher : Central Basic Biomedical and Health Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (466.555 KB)

Abstract

Embryonic Stem Cells (ESCs) is a one of source for pluripotent stem cell that capable to differentiate into various cell types. This has opened up opportunities utilization of embryos as a source of stem cells. However, the use of embryos as objects of research still has ethical constraints. Currently parthenogenetic embryo in the blastocyst stage is considered to be one of the alternative sources of ESC are "ethical" because its obtained not from the fertilization of the oocyte and sperm. Parthenogenetic embryo derived from oocyte that activation in vitro to obtain embryos in the blastocyst stage. This research aims to produce blastocysts parthenogenetic mice as a source of ESC. In this study super ovulation performed on Swiss Webster female mice to obtain oocytes. Activation of mouse oocytes done by culturing oocytes in medium with 10 mM strontium chloride (SrCl2) and 5 mg / ml cytokalasin B for 6 hours and cultured for 4-5 days to get the embryo parthenogenetic. The results showed that development of mice oocyte activated to be 2 pronucleus (2PN), cleavage, morula and blastocyst sequence was 65%, 97%, 90% and 23%. The conclusion of this study indicate that the parthenogenetic embryos as sources of stem cells can be produced in vitro.Key words: stem cells, embryonic stem cells (ESCs), blastocyst, parthenogenetic AbstrakEmbryonic Stem Cells (ESCs) merupakan salah satu satu sumber sel punca (stem cell) yang bersifat pluripoten karena kemampuannya berdiferensiasi menjadi berbagai tipe sel. Hal inilah yang membuka peluang pemanfaatan embrio sebagai sumber stem cell. Namun pemanfaatan embrio sebagai objek penelitian masih mempunyai kendala etik. Saat ini embrio partenogenetik tahap blastosis dianggap dapat menjadi salah satu alternatif sumber ESC yang “etis” karena diperoleh bukan dari hasil fertilisasi antara oosit dan sperma. Embrio parthenogenetik diperoleh dari aktivasi oosit secara in vitro untuk memperoleh embrio tahap blastosis. Penelitian ini bertujuan memproduksi blastosis partenogenetik mencit sebagai sumber ESC. Pada penelitian ini superovulasi dilakukan pada mencit betina Swis webster untuk mendapatkan oosit. Aktivasi oosit mencit dilakukan dengan mengkultur oosit dalam medium dengan 10mM strontium chloride (SrCl2) dan 5 μg/ml cytokalasin B selama 6 jam dan kultur selama 4-5 hari untuk mendapatkan embrio partenogenetik. Hasil penelitian menunjukkan bahwa perkembangan oosit mencit yang telah teraktivasi menjadi tahap 2 pronukleus (2PN), cleavage, morula dan blastosis secara berurutan adalah 65%, 97%, 90% dan 23%. Kesimpulan dari penelitian ini menunjukkan bahwa embrio partenogenetik sebagai sumber stem cell dapat diproduksi secara in vitro.Kata kunci: Sel punca (stem cell), sel punca embrionik (ESCs), blastosis, partenogenetik
Ulasan Sistematik: Marka Molekular Penanda Patogenitas dan Sebaran Inang Pada Virus Avian Influenza H5N1 Adam, Kindi; Wulandari, R.Aj. Sri
Jurnal Biotek Medisiana Indonesia Vol 2, No 1 (2013)
Publisher : Central Basic Biomedical and Health Technology

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Abstract

The epidemic arising out of H5N1 virus infection causes death case and material loss. Mutation of H5N1 virus that signify the increase of pathogenicity and change of dispersal of host response can be used to reference for early warning system of epidemic caused of avian influenza virus. The objective of this research is to obtain information related to molecular markers of pathogenicity of avian influenza. Reference searching is use to collect information about molecular markers related to pathogenicity. The keywords used on this study are: Avian influenza, H5N1, mutation, Pathogenicity, HA, NA, PB, PA, and NS. Thirty three research papers, 4 reviews and 1 scientific seminar are used on this research. The HA, NA and NS genes was reported to be an important gene that have molecular marker related to increase ofpathogenicity. Whereas HA, PB1, PB2 and PA genes was related to the adaptive ability and the dispersal of host of avian influenza virus. There is 30 amino acid that sign as molecular markers of pathogenicity of avian influenza H5N1. Mutation on 30 molecular markers of HA, NA, PB and NS genes can be used as predictor to anticipate mutation orientation of avian influenza virus to become highly pathogenic and host alteration.Thereby, virus mutation to become highly pathogenic can be anticipated early.Keybwords : Highly pathogenic avian Influenza, Mutation, Molecular Marker, Pathogenicity. HA, NA, PB, NS AbstrakEpidemi akibat virus H5N1 telah menimbulkan banyak korban jiwa dan materi.Mutasi virus H5N1 yang menandakan peningkatan patogenitas dan perubaan sebaran inang dapat dijadikan acuan untuk sistem deteksi dini kewaspadaan epidemi akibat virus avian influenza. Penelitian ini bertujuan mendapatkan informasi terkait dengan marka molekular penanda patogenitas virus avian influenza H5N1. Ulasan sistematis ini dilakukan dengan penelusuran literatur menggunakan Google scholar dan PubMed. Kata pencarian yang digunakan adalah Avian influenza, H5N1, mutation, Pathogenicity, HA,NA,PB,PA,NS. Referensi yang digunakan adalah 33 jurnal, 4 ulasan penelitian dan 1 seminar ilmiah dari dalam dan luar negeri. Gen HA, NA dan NS merupakan gen penting yang memiliki penanda molekuler yang berkaitan dengan peningkatan patogenitas virus avian influenza. Sedangkan gen PB1, PB2 dan PA berhubungan dengan adaptasi dan perubahan rentang inang. Jumlah penanda patogenitas pada gen HA, NA, PB dan NS adalah 30 asam amino. Mutasi pada 30 marka genetik gen HA, NA, PB dan NSmerupakan penanda molekular yang dapat digunakan sebagai panduan awal untuk mengantisipasi arah mutasi virus avian influenza menuju pergeseran rentang inang dan peningkatan patogenitasnya. Dengan demikian, perubahan virus avian influenza menjadi bersifat higly pathogenic dapat diantisipasi lebih dini.Kata kunci : Highly pathogenicavian Influenza, Mutasi,Marka Molekular, Patogenitas., HA, NA, PB, NS.
The Surprising Complexity of Virus-Host Cell Interaction Revealed by the Powerful Systems Biology Approaches of Genomics and Proteomics Rustanti, Lina
Jurnal Biotek Medisiana Indonesia Vol 2, No 1 (2013)
Publisher : Central Basic Biomedical and Health Technology

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Abstract

Understanding interaction between viruses and host cells during infection process is the first step in discovering appropriate drugs and vaccines against viral diseases. Advance technologies based on genomics and proteomics approaches provide great tools to disclose the complexity of virus-host interaction. In this essay, the application of RNAi screens method and proteomics-based approaches on influenza virus will be elucidated as an example. Using those methods, the primary factors controlling viral replication pathway were discovered. These findings are useful for the development of potential strategies to overcome viral diseases.Keywords : virus, host, pathogen, interaction, genomic, proteomic. AbstrakMemahami interaksi antara virus dan pejamu dalam proses infeksi merupakan langkah awal dalam upaya penemuan obat dan vaksin yang tepat untuk melawan penyakit yang disebabkan oleh virus. Pendekatan teknologi berbasis genomik dan proteomik merupakan terobosan yang dapat menjawab kompleksitas interaksi virus-pejamu. Dalam kajian ini, akan dibahas penerapan teknologi screening RNAi dan teknologi berbasis proteomik pada virus influenza. Dengan menggunakan metode ini, faktor-faktor utama yang mengontrol tahap replikasi virus akan dapat ditemukan. Penemuan ini sangat bermanfaat dalam pengembangan strategi pengobatan yang potensial untuk mengatasi penyakit bersumber virus.Kata kunci: virus, pejamu, patogen, interaksi, genomik, proteomik.
Perilaku Bertelur Nyamuk Aedes aegypti pada Media Air Tercemar Wurisastuti, Tri
Jurnal Biotek Medisiana Indonesia Vol 2, No 1 (2013)
Publisher : Central Basic Biomedical and Health Technology

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Abstract

The theory is believed that the mosquito Aedes aegypti does not like laying in a pool of contaminated water. However, some studies indicate a change in behavior of these mosquitoes breed. Authors conducted follow-up analysis of a descriptive study to determine whether the mosquito Aedes aegypti willing to lay their eggs in different media polluted water and polluted water knows that most preferably Aedes aegypti mosquitoes to lay eggs. Advanced analysis of R & D performed in Loka P2B2 Baturaja with the type of non-intervention and observational studies using analysis of variance (ANOVA) and Duncan test. Software used SAS 9.1. Analysis of variance showed that different types of water media have a real influence on the preference of Aedes aegypti mosquitoes lay their eggs on the 5% significance level with p-value of <0.0001. Cow manure contaminated water is the most preferred medium of Aedes aegypti mosquitoes lay their eggs.Keywords: medium water, eggs, Aedes aegypty, analysis of variance AbstrakTeori yang ada meyakini bahwa nyamuk Aedes aegypti tidak suka bertelur di genangan air tercemar. Namun beberapa penelitian menunjukkan adanya perubahan perilaku berkembang biak nyamuk tersebut. Penulis melakukan analisis lanjutan dari penelitian deskriptif dengan untuk mengetahui apakah nyamuk Aedes aegypti mau bertelur di berbagai media air tercemar dan mengetahui air tercemar yang paling disukai nyamuk Aedes aegypti untuk bertelur. Analisis lanjut dilakukan di Loka Litbang P2B2 Baturaja dengan jenis penelitian observasi non intervensi dan menggunakan analysis of varians (ANOVA) serta uji lanjut Duncan. Software yang digunakan SAS 9.1. Analysis of varians menunjukkan bahwa berbagai jenis media air memiliki pengaruh yang nyata terhadap kesukaan bertelur nyamuk Aedes aegypti pada taraf nyata 5% dengan nilai-p sebesar < 0.0001. Air yang tercemar kotoran sapi merupakan media yang paling disukai nyamuk Aedes aegypti meletakkan telurnya.Kata Kunci: media air, telur, Aedes aegypty, analysis of varians
Distribusi Frekuensi Beberapa Subtipe Human Immunodeficiency Virus-1 (HIV-1) di Provinsi Kalimantan Barat Tahun 2010 -, Roselinda
Jurnal Biotek Medisiana Indonesia Vol 2, No 1 (2013)
Publisher : Central Basic Biomedical and Health Technology

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Abstract

HIV-1 subtype distribution in the province of West Kalimantan with a sufficient amount of data has not been so well known, so is the predominant subtype and proportion. The purpose of the analysis is to look at distribution of HIV-1 subtypes circulating in West Kalimantan province with risk factors. The data analyzed came from surveys of HIV-1 virus genotypes in West Kalimantan in 2010 in the case of HIV/AIDS chosen purposively who visited the clinic Voluntary Care and Treatment (VCT) in Pontianak and Singkawang. For each city chosen purposively 1 hospital. Blood specimens were taken from patients to be examined by PCR and sequencing, then the data sociodemographic and medical history interview was conducted with a structured questionnaire after obtaining consent from the patient of the 80 serum specimens examined by PCR and sequencing subtype CRF01_AE obtained by 83.4%. Also found subtype E of 10.4%, AC subtypes, A and C, respectively 2.1% . Highest proportion of subtype CRF01_AE was found in the group that has the status of a widow/not married, the group with the Malays and the age group 15-30 years. Most transmission through IDU history. HIV-1 subtype CRF01_AE is predominant subtype recombinant circulating in the province of West Kalimantan.Keywords: HIV-1 subtypes, West Kalimantan. AbstrakDistribusi subtipe HIV-1 di Provinsi Kalimantan Barat dengan jumlah data yang memadai belum begitu diketahui, begitu juga subtipe yang dominant dan proporsinya. Tujuan analisis ialah untuk melihat distribusi subtipe HIV-1 yang beredar di Propinsi Kalimantan Barat dengan faktor risikonya. Data yang dianalisis berasal dari survei Genotipe virus HIV-1 di Provinsi Kalimantan Barat tahun 2010 pada kasus HIV atau AIDS dipilih purposif yang mengunjungi klinik Perawatan Sukarela dan Pengobatan (VCT) di Pontianak dan Singkawang. Untuk setiap kota dipilih 1 rumah sakit secara purposif. Spesimen darah diambil dari penderita untuk diperiksa dengan tes PCR dan sekuensing, kemudian data sosiodemografi dan riwayat penyakit dilakukan wawancara dengan kuesioner terstruktur setelah mendapat persetujuan dari penderita. Dari 80 spesimen serum yang diperiksa dengan PCR dan sekuensing didapatkan subtipe CRF01_AE sebesar 83,4%. Selain itu ditemukan subtipe E sebesar 10,4%, subtipe AC, A dan C masing-masing 2,1%. Proporsi terbanyak subtipe CRF01_AE ditemukan pada kelompok yang mempunyai status janda atau tidak menikah, kelompok dengan suku Melayu dan pada kelompok dengan usia 15- 30 tahun. Riwayat penularan terbanyak melalui IDU. Subtipe CRF01_AE HIV-1 merupakan subtipe rekombinan yang dominan beredar di Provinsi Kalimantan Barat.Kata Kunci: subtipe HIV-1, Kalimantan Barat.

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