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INDONESIA
Jurnal Hortikultura
Published by Kementerian Pertanian
ISSN : 08537097     EISSN : 25025120     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Jurnal Hortikultura (J.Hort) memuat artikel primer yang bersumber dari hasil penelitian hortikultura, yaitu tanaman sayuran, tanaman hias, tanaman buah tropika maupun subtropika. Jurnal Hortikultura diterbitkan oleh Pusat Penelitian dan Pengembangan Hortikultura, Badan Litbang Pertanian, Kementerian Pertanian. Jurnal Hortikultura terbit pertama kali pada bulan Juni tahun 1991, dengan empat kali terbitan dalam setahun, yaitu setiap bulan Maret, Juni, September, dan Desember.
Arjuna Subject : -
Articles 8 Documents
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Search results for , issue "Vol 16, No 2 (2006): Juni 2006" : 8 Documents clear
Induksi dan Regenerasi Embriogenesis Somatik Pepaya Sutanto, Agus; Aziz, M A
Jurnal Hortikultura Vol 16, No 2 (2006): Juni 2006
Publisher : Indonesian Center for Horticultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Penelitian ini bertujuan menginduksi dan meregenerasi embrio somatik dari embrio zigotik buah pepaya muda kultivar Eksotika II. Kegiatan dilaksanakan di laboratorium Kultur Jaringan Jabatan Sains Tanaman, Universiti Putra Malaysia pada bulan Januari sampai Agustus 2001. Penelitian ini menggunakan rancangan acak lengkap dengan perlakuan kombinasi beberapa konsentrasi 2,4-D (1,0; 2,5 dan 5,0 mg/l) dan BAP (0; 0,001; 0,005; dan 0,01 mg/l). Hasil penelitian menunjukkan bahwa media MS yang mengandung 5,0 mg/l 2,4-D membentuk embrio somatik tertinggi (74,55%) dan kombinasi dari 2,4-D 1,0 mg/l dan BAP 0,01 mg/l menghasilkan kalus tertinggi (52,58%) pada 8 minggu setelah kultur. Jumlah embrio somatik per eksplan terbanyak (66,61) diperoleh pada media MS dengan 5,0 mg/l 2,4-D dan 0,01 mg/l BAP. Pemasakan embrio diperoleh dengan memindahkan embrio globular ke media padat MS tanpa zat pengatur tumbuh. Empat minggu setelah kultur, 42% embrio somatik berkecambah setelah embrio masak. Plantlet siap diaklimatisasi pada 8 minggu setelah kultur ke media perkecambahan. Sistem perbanyakan dan regenerasi embrio somatik pepaya ini dapat menunjang keberhasilan pemuliaan tanaman pepaya modern melalui transformasi genetikThe experiments with the objectives to induce and regenerate somatic embryogenesis were carried out at the Tissue Culture Laboratory of Crop Science Department, Universiti Putra Malaysia from January to August 2001. The experiments involved the induction of somatic embryogenesis from immature zygotic embryos of papaya cv. Eksotika II and regeneration of plantlets from the somatic embryos. A nonfactorial completely randomized design was used as experimental design with treatments of the 2.4-D combinations concentrations (1.0; 2.5 and 5.0 mg/l) and BAP concentrations (0; 0.001; 0.005 and 0.01 mg/l). The results showed that MS medium supplemented with 2,4-D 5.0 mg/l promoted the highest percentage of somatic embryogenesis (74.55%), while the combination of 1.0 mg/l 2,4-D and 0.01 mg/l BAP produced the highest percentage of callus formation (52.58%) after 8 weeks of culture. The highest number of somatic embryos per explant (66.61) was obtained when 5.0 mg/l 2,4-D and 0.01 mg/l BAP were added into MS medium. Maturation of somatic embryos was achieved on transfering the globular somatic embryos derived from zigotic embryo to solid MS medium without growth regulator. After 4 weeks of culture, 42% germinated somatic embryo was occurred following maturation of somatic embryos. Plantlets were ready for acclimatization to germination medium 8 weeks after culture. Somatic embryogenesis system could enhance the successful of the modern papaya breeding program through genetic transformation.
Perbaikan Produksi Jamur Tiram dengan Modifikasi Bahan Baku Utama Media Bibit Sumiati, Ety; Suryaningsih, E; Puspitasati, -
Jurnal Hortikultura Vol 16, No 2 (2006): Juni 2006
Publisher : Indonesian Center for Horticultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Faktor budidaya jamur tiram yang terpenting adalah aplikasi media bibit induk yang berkualitas. Penelitian dilakukan di laboratorium Ekofisiologi Balai Penelitian Tanaman Sayuran, Lembang (1.250 m dpl.) dari bulan September 2003 sampai Maret 2004.Tujuan penelitian untuk mendapatkan jenis bahan baku media bibit jamur tiram dan lama waktu perebusan yang terbaik sebelum disusun sebagai formula media bibit. Kriteria kualitas media bibit dinyatakan dalam satuan waktu, yaitu kecepatan waktu awal dan waktu akhir miselium tumbuh memenuhi botol wadah media bibit. Rancangan percobaan menggunakan RAK pola faktorial dengan 2 ulangan. Faktor pertama lama waktu perebusan awal bahan baku. Faktor kedua jenis bahan baku utama media terdiri atas 21 jenis. Formula media bibit induk juga menggunakan standar formula petani. Bibit jamur tiram menggunakan spesies Pleurotus ostreatus strain Florida dari Applied Plant Research, Belanda. Hasil penelitian mengungkapkan bahwa hampir semua jenis bahan baku yang dicoba dapat digunakan sebagai media bibit kecuali biji oat. Bahan baku utama media bibit induk jamur tiram yang terbaik adalah millet + SKG 1:1, yaitu  menghasilkan koloni miselium yang kompak, berwarna putih bersih, dan pertumbuhannya konsisten. Bahan baku SKG, beras merah, dan millet tidak perlu direbus, sedangkan oat, wheat, jali lokal tidak dikupas, jali impor, kacang merah, dan kacang hijau perlu direbus selama 15-30 menit.The  very  important step in oyster mushrooms cultivation is application of high quality  of  spawn media. The study was conducted at Ecophysiology Lab. Indonesian Vegetables Research Institute in Lembang (1,250 m asl) from September 2003 to March 2004. The goals of this experiment were to get proper kinds of raw materials for spawn media, and the duration of media sterilization. Criteria of good raw materials for spawn media is expressed in minimum total days needed for the growth of mycelium to cover the spawn media (full colonization). A randomized block design with a factorial pattern was set up. The first factor was duration of sterilization by boiling of main raw materials for 0, 15, 30, and 45 minutes, respectively. The second factor was 21kinds of raw materials including formula for spawn media used by farmers. All treatment combinations were replicated 2 times. Oyster mushroom species used in the experiment was white Pleurotus ostreatus strain  Florida from Applied Plant Research, Netherlands. Research results revealed that in general almost all kinds of raw materials and their combinations were suitable for spawn media of oyster mushroom, except oat grains. The best raw material for spawn media was millet grains+sawdust 1:1. This combination gave the highest quality of mycelium performance with compact colonization, consistent growth, and very clean-white mycelium. Sawdust, red rice, and millet were the best spawn media and can be used directly, while oat, wheat, local jali, imported jali, red bean, and mungbean can be used through 15-30 minutes sterilization.
Pertumbuhan dan Hasil 20 Progeni Kentang Asal Biji Botani di Dataran Tinggi Pangalengan, Jawa Barat Gunadi, Nirkadi
Jurnal Hortikultura Vol 16, No 2 (2006): Juni 2006
Publisher : Indonesian Center for Horticultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Percobaan untuk mengetahui pertumbuhan dan hasil umbi dari 20 progeni TPS baru dari CIP-Lima, Peru telah dilaksanakan di Desa Padaawas (1.400 m dpl.), Pangalengan, Jawa Barat dari bulan Agustus sampai Desember 2004. Umbi semaian 20 progeni TPS baru, ditanam pada petak-petak percobaan yang diatur dalam rancangan acak kelompok dengan ulangan 4 kali. Kentang kultivar Granola digunakan sebagai kontrol. Hasil penelitian menunjukkan bahwa 4 progeni TPS, yaitu AL-624 x TPS-67, CFK-69-1 x TPS-67, MF-II x C95LB-13.2, dan MF-II x TPS-67 memberikan hasil umbi yang lebih tinggi dibandingkan dengan hasil umbi dari progeni-progeni lainnya. Hasil umbi per tanaman dari keempat progeni TPS tersebut sebanding dengan hasil umbi per tanaman dari kultivar Granola. Dua progeni yaitu AL-624 x TPS-67 dan CFK-69-1 x TPS-67 juga memberikan hasil umbi per ha sebanding dengan hasil umbi per ha kultivar Granola. Hasil penelitian ini dapat digunakan sebagai acuan untuk pemilihan progeni TPS dalam menggunakan TPS sebagai alternatif bahan tanam dalam produksi kentang, selain umbi bibit tradisional.An onfarm experiment to determine the plant growth and tuber yields of 20 new TPS progenies from CIP-Lima, Peru was conducted in Padaawas, 1,400 m asl., Pangalengan, West Java from August to December 2004. Seedling tuber of 20 new TPS progenies were grown in the experimental plots, which were arranged in a randomized complete block design with 4 replications. The common cultivar of cv. Granola was used as control. The results indicated that 4 progenies of AL-624 x TPS-67, CFK-69-1 x TPS-67, MF-II x C95LB-13.2, and MF-II x TPS-67 had the higher tuber yields than those of other progenies. These 4 progenies had comparable tuber yields per plant to that of cv. Granola. Two progenies of AL-624 x TPS-67 and CFK-69-1 x TPS-67 had also comparable tuber yields per ha to that of cv. Granola. The results could be used as a recommendation in order to select TPS progenies as alternative planting material in potato production using TPS other than traditional seed tuber.
Perbaikan Produksi Jamur Tiram Pleurotus ostreatus Strain Florida dengan Modifikasi Bahan Baku Utama Substrat Sumiati, Ety; Suryaningsih, E; Puspitasari, -
Jurnal Hortikultura Vol 16, No 2 (2006): Juni 2006
Publisher : Indonesian Center for Horticultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Daya hasil jamur tiram putih masih perlu diperbaiki. Penelitian bertujuan mendapatkan jenis bahan baku alternatif selain serbuk kayu gergaji albasia dengan penambahan bahan aditif berupa bekatul untuk budidaya jamur tiram putih. Percobaan menggunakan rancangan petak terpisah  dengan 3 ulangan. Petak utama adalah bekatul konsentrasi 5, 10, 15, dan 20%. Anak petak 13 jenis bahan baku utama substrat. Bibit jamur tiram putih menggunakan spesies Pleurotus ostreatus strain Florida yang diintroduksi dari Applied Plant Research, Belanda. Penelitian dilakukan di Balai Penelitian Tanaman Sayuran , Lembang  (1.250 m dpl.), Jawa Barat dari bulan November 2003 sampai Mei 2004. Hasil penelitian mengungkapkan bahwa aplikasi substrat serbuk kayu gergaji albasia dikombinasikan dengan bekatul 5% menghasilkan bobot segar jamur tiram putih tertinggi, yaitu 2.317,36 g/kg bobot substrat basah dengan efisiensi biologis  (EB) 81,03%. Hasil tersebut dicapai selama  65 hari  masa berproduksi dengan jumlah panen 12 kali. Selain serbuk kayu gergaji (SKG) albasia, bahan baku substrat berupa SKG campuran, daun pisang kering, jerami padi, rumput alang-alang, dan bagas/ampas tebu dengan penambahan bekatul antara 5-15%, juga merupakan bahan baku alternatif yang dapat digunakan sebagai baku substrat untuk budidaya jamur tiram putih. Hasil bobot segar jamur tiram dari berbagai jenis bahan baku alternatif, yaitu antara 600 sampai 1.200 g/kg bobot basah substrat dengan nilai EB antara 10 sampai 35%The goal of this experiment was to find out alternatively suitable materials for substrates  other than sawdust combined with the application of rice bran as additive materials. A split plot design with 3 replications was set up. Main plot was rice bran concentrations of 5, 10, 15, and 20%, respectively. Subplot was 13 kinds of substrate materials. The experiment was carried out at Indonesian Vegetable Research Institute in Lembang (1,250 m asl), West Java from November 2003 to May 2004. Oyster mushroom species used in the experiment was Pleurotus ostreatus strain Florida from Applied Plant Research, The Netherlands. The results of the experiment revealed that application of sawdust albizia in combination with 5% of rice bran produced the highest fresh yield of white oyster mushroom, viz. 2 317.36 g/kg wet substrate with biological efficiency (BE) of 81.03%. This yield was produced during 65 days with  12 times of total number of harvests. Other kinds of substrates, viz. mixed sawdust, dry banana leaves,  rice straw, sedge grass leaves, and sugar cane bagasse in combination with rice bran of 5 to 15%, could be applied as  alternative substrate materials other than sawdust of albizia for cultivating white oyster mushroom. The yield of fresh oyster mushroom using those kinds of alternative substrates mentioned, viz.  600 to 1,200 g/kg wet substrate with BE of 10 to 35%.
Induksi dan Regenerasi Embriogenesis Somatik Pepaya Agus Sutanto; M A Aziz
Jurnal Hortikultura Vol 16, No 2 (2006): Juni 2006
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v16n2.2006.p%p

Abstract

Penelitian ini bertujuan menginduksi dan meregenerasi embrio somatik dari embrio zigotik buah pepaya muda kultivar Eksotika II. Kegiatan dilaksanakan di laboratorium Kultur Jaringan Jabatan Sains Tanaman, Universiti Putra Malaysia pada bulan Januari sampai Agustus 2001. Penelitian ini menggunakan rancangan acak lengkap dengan perlakuan kombinasi beberapa konsentrasi 2,4-D (1,0; 2,5 dan 5,0 mg/l) dan BAP (0; 0,001; 0,005; dan 0,01 mg/l). Hasil penelitian menunjukkan bahwa media MS yang mengandung 5,0 mg/l 2,4-D membentuk embrio somatik tertinggi (74,55%) dan kombinasi dari 2,4-D 1,0 mg/l dan BAP 0,01 mg/l menghasilkan kalus tertinggi (52,58%) pada 8 minggu setelah kultur. Jumlah embrio somatik per eksplan terbanyak (66,61) diperoleh pada media MS dengan 5,0 mg/l 2,4-D dan 0,01 mg/l BAP. Pemasakan embrio diperoleh dengan memindahkan embrio globular ke media padat MS tanpa zat pengatur tumbuh. Empat minggu setelah kultur, 42% embrio somatik berkecambah setelah embrio masak. Plantlet siap diaklimatisasi pada 8 minggu setelah kultur ke media perkecambahan. Sistem perbanyakan dan regenerasi embrio somatik pepaya ini dapat menunjang keberhasilan pemuliaan tanaman pepaya modern melalui transformasi genetikThe experiments with the objectives to induce and regenerate somatic embryogenesis were carried out at the Tissue Culture Laboratory of Crop Science Department, Universiti Putra Malaysia from January to August 2001. The experiments involved the induction of somatic embryogenesis from immature zygotic embryos of papaya cv. Eksotika II and regeneration of plantlets from the somatic embryos. A nonfactorial completely randomized design was used as experimental design with treatments of the 2.4-D combinations concentrations (1.0; 2.5 and 5.0 mg/l) and BAP concentrations (0; 0.001; 0.005 and 0.01 mg/l). The results showed that MS medium supplemented with 2,4-D 5.0 mg/l promoted the highest percentage of somatic embryogenesis (74.55%), while the combination of 1.0 mg/l 2,4-D and 0.01 mg/l BAP produced the highest percentage of callus formation (52.58%) after 8 weeks of culture. The highest number of somatic embryos per explant (66.61) was obtained when 5.0 mg/l 2,4-D and 0.01 mg/l BAP were added into MS medium. Maturation of somatic embryos was achieved on transfering the globular somatic embryos derived from zigotic embryo to solid MS medium without growth regulator. After 4 weeks of culture, 42% germinated somatic embryo was occurred following maturation of somatic embryos. Plantlets were ready for acclimatization to germination medium 8 weeks after culture. Somatic embryogenesis system could enhance the successful of the modern papaya breeding program through genetic transformation.
Perbaikan Produksi Jamur Tiram dengan Modifikasi Bahan Baku Utama Media Bibit Ety Sumiati; E Suryaningsih; - Puspitasati
Jurnal Hortikultura Vol 16, No 2 (2006): Juni 2006
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v16n2.2006.p%p

Abstract

Faktor budidaya jamur tiram yang terpenting adalah aplikasi media bibit induk yang berkualitas. Penelitian dilakukan di laboratorium Ekofisiologi Balai Penelitian Tanaman Sayuran, Lembang (1.250 m dpl.) dari bulan September 2003 sampai Maret 2004.Tujuan penelitian untuk mendapatkan jenis bahan baku media bibit jamur tiram dan lama waktu perebusan yang terbaik sebelum disusun sebagai formula media bibit. Kriteria kualitas media bibit dinyatakan dalam satuan waktu, yaitu kecepatan waktu awal dan waktu akhir miselium tumbuh memenuhi botol wadah media bibit. Rancangan percobaan menggunakan RAK pola faktorial dengan 2 ulangan. Faktor pertama lama waktu perebusan awal bahan baku. Faktor kedua jenis bahan baku utama media terdiri atas 21 jenis. Formula media bibit induk juga menggunakan standar formula petani. Bibit jamur tiram menggunakan spesies Pleurotus ostreatus strain Florida dari Applied Plant Research, Belanda. Hasil penelitian mengungkapkan bahwa hampir semua jenis bahan baku yang dicoba dapat digunakan sebagai media bibit kecuali biji oat. Bahan baku utama media bibit induk jamur tiram yang terbaik adalah millet + SKG 1:1, yaitu  menghasilkan koloni miselium yang kompak, berwarna putih bersih, dan pertumbuhannya konsisten. Bahan baku SKG, beras merah, dan millet tidak perlu direbus, sedangkan oat, wheat, jali lokal tidak dikupas, jali impor, kacang merah, dan kacang hijau perlu direbus selama 15-30 menit.The  very  important step in oyster mushrooms cultivation is application of high quality  of  spawn media. The study was conducted at Ecophysiology Lab. Indonesian Vegetables Research Institute in Lembang (1,250 m asl) from September 2003 to March 2004. The goals of this experiment were to get proper kinds of raw materials for spawn media, and the duration of media sterilization. Criteria of good raw materials for spawn media is expressed in minimum total days needed for the growth of mycelium to cover the spawn media (full colonization). A randomized block design with a factorial pattern was set up. The first factor was duration of sterilization by boiling of main raw materials for 0, 15, 30, and 45 minutes, respectively. The second factor was 21kinds of raw materials including formula for spawn media used by farmers. All treatment combinations were replicated 2 times. Oyster mushroom species used in the experiment was white Pleurotus ostreatus strain  Florida from Applied Plant Research, Netherlands. Research results revealed that in general almost all kinds of raw materials and their combinations were suitable for spawn media of oyster mushroom, except oat grains. The best raw material for spawn media was millet grains+sawdust 1:1. This combination gave the highest quality of mycelium performance with compact colonization, consistent growth, and very clean-white mycelium. Sawdust, red rice, and millet were the best spawn media and can be used directly, while oat, wheat, local jali, imported jali, red bean, and mungbean can be used through 15-30 minutes sterilization.
Pertumbuhan dan Hasil 20 Progeni Kentang Asal Biji Botani di Dataran Tinggi Pangalengan, Jawa Barat Gunadi, Nirkadi
Jurnal Hortikultura Vol 16, No 2 (2006): Juni 2006
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v16n2.2006.p%p

Abstract

Percobaan untuk mengetahui pertumbuhan dan hasil umbi dari 20 progeni TPS baru dari CIP-Lima, Peru telah dilaksanakan di Desa Padaawas (1.400 m dpl.), Pangalengan, Jawa Barat dari bulan Agustus sampai Desember 2004. Umbi semaian 20 progeni TPS baru, ditanam pada petak-petak percobaan yang diatur dalam rancangan acak kelompok dengan ulangan 4 kali. Kentang kultivar Granola digunakan sebagai kontrol. Hasil penelitian menunjukkan bahwa 4 progeni TPS, yaitu AL-624 x TPS-67, CFK-69-1 x TPS-67, MF-II x C95LB-13.2, dan MF-II x TPS-67 memberikan hasil umbi yang lebih tinggi dibandingkan dengan hasil umbi dari progeni-progeni lainnya. Hasil umbi per tanaman dari keempat progeni TPS tersebut sebanding dengan hasil umbi per tanaman dari kultivar Granola. Dua progeni yaitu AL-624 x TPS-67 dan CFK-69-1 x TPS-67 juga memberikan hasil umbi per ha sebanding dengan hasil umbi per ha kultivar Granola. Hasil penelitian ini dapat digunakan sebagai acuan untuk pemilihan progeni TPS dalam menggunakan TPS sebagai alternatif bahan tanam dalam produksi kentang, selain umbi bibit tradisional.An onfarm experiment to determine the plant growth and tuber yields of 20 new TPS progenies from CIP-Lima, Peru was conducted in Padaawas, 1,400 m asl., Pangalengan, West Java from August to December 2004. Seedling tuber of 20 new TPS progenies were grown in the experimental plots, which were arranged in a randomized complete block design with 4 replications. The common cultivar of cv. Granola was used as control. The results indicated that 4 progenies of AL-624 x TPS-67, CFK-69-1 x TPS-67, MF-II x C95LB-13.2, and MF-II x TPS-67 had the higher tuber yields than those of other progenies. These 4 progenies had comparable tuber yields per plant to that of cv. Granola. Two progenies of AL-624 x TPS-67 and CFK-69-1 x TPS-67 had also comparable tuber yields per ha to that of cv. Granola. The results could be used as a recommendation in order to select TPS progenies as alternative planting material in potato production using TPS other than traditional seed tuber.
Perbaikan Produksi Jamur Tiram Pleurotus ostreatus Strain Florida dengan Modifikasi Bahan Baku Utama Substrat Ety Sumiati; E Suryaningsih; - Puspitasari
Jurnal Hortikultura Vol 16, No 2 (2006): Juni 2006
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v16n2.2006.p%p

Abstract

Daya hasil jamur tiram putih masih perlu diperbaiki. Penelitian bertujuan mendapatkan jenis bahan baku alternatif selain serbuk kayu gergaji albasia dengan penambahan bahan aditif berupa bekatul untuk budidaya jamur tiram putih. Percobaan menggunakan rancangan petak terpisah  dengan 3 ulangan. Petak utama adalah bekatul konsentrasi 5, 10, 15, dan 20%. Anak petak 13 jenis bahan baku utama substrat. Bibit jamur tiram putih menggunakan spesies Pleurotus ostreatus strain Florida yang diintroduksi dari Applied Plant Research, Belanda. Penelitian dilakukan di Balai Penelitian Tanaman Sayuran , Lembang  (1.250 m dpl.), Jawa Barat dari bulan November 2003 sampai Mei 2004. Hasil penelitian mengungkapkan bahwa aplikasi substrat serbuk kayu gergaji albasia dikombinasikan dengan bekatul 5% menghasilkan bobot segar jamur tiram putih tertinggi, yaitu 2.317,36 g/kg bobot substrat basah dengan efisiensi biologis  (EB) 81,03%. Hasil tersebut dicapai selama  65 hari  masa berproduksi dengan jumlah panen 12 kali. Selain serbuk kayu gergaji (SKG) albasia, bahan baku substrat berupa SKG campuran, daun pisang kering, jerami padi, rumput alang-alang, dan bagas/ampas tebu dengan penambahan bekatul antara 5-15%, juga merupakan bahan baku alternatif yang dapat digunakan sebagai baku substrat untuk budidaya jamur tiram putih. Hasil bobot segar jamur tiram dari berbagai jenis bahan baku alternatif, yaitu antara 600 sampai 1.200 g/kg bobot basah substrat dengan nilai EB antara 10 sampai 35%The goal of this experiment was to find out alternatively suitable materials for substrates  other than sawdust combined with the application of rice bran as additive materials. A split plot design with 3 replications was set up. Main plot was rice bran concentrations of 5, 10, 15, and 20%, respectively. Subplot was 13 kinds of substrate materials. The experiment was carried out at Indonesian Vegetable Research Institute in Lembang (1,250 m asl), West Java from November 2003 to May 2004. Oyster mushroom species used in the experiment was Pleurotus ostreatus strain Florida from Applied Plant Research, The Netherlands. The results of the experiment revealed that application of sawdust albizia in combination with 5% of rice bran produced the highest fresh yield of white oyster mushroom, viz. 2 317.36 g/kg wet substrate with biological efficiency (BE) of 81.03%. This yield was produced during 65 days with  12 times of total number of harvests. Other kinds of substrates, viz. mixed sawdust, dry banana leaves,  rice straw, sedge grass leaves, and sugar cane bagasse in combination with rice bran of 5 to 15%, could be applied as  alternative substrate materials other than sawdust of albizia for cultivating white oyster mushroom. The yield of fresh oyster mushroom using those kinds of alternative substrates mentioned, viz.  600 to 1,200 g/kg wet substrate with BE of 10 to 35%.

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