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INDONESIA
Jurnal AgroBiogen
Published by Kementerian Pertanian
ISSN : 19071094     EISSN : 25491547     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Jurnal AgroBiogen memuat artikel primer dan sekunder hasil penelitian bioteknologi dan sumberdaya genetik tanaman, serangga, dan mikroba pertanian. Jurnal ini diterbitkan tiga kali setahun pada bulan April, Agustus dan Oktober oleh Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
Arjuna Subject : -
Articles 5 Documents
 1 
Search results for , issue "Vol 11, No 3 (2015): Desember" : 5 Documents clear
Gen dan QTL Pengendali Toleransi Tanaman terhadap Keracunan Aluminium dan Aplikasinya untuk Pemuliaan Tanaman di Indonesia I Made Tasma
Jurnal AgroBiogen Vol 11, No 3 (2015): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v11n3.2015.p111-124

Abstract

Genetic knowledge of loci controlling Al toxicity tolerance is the key for a successful breeding program in developing Altolerant cultivars. Tolerance level of crop plants to Al toxicity is genetically controlled. The gene inheritance pattern is mainlyresulted from intensive studies of cereal crops, such as wheat, sorghum, maize, and rice. The trait can be controlled by asingle dominant gene, a single dominant gene with many alleles, a pair of dominant genes, or by many genes (QTL). Themajority of the Al tolerance genes identified so far belongs to two independent groups of gene families, i.e. aluminumactivatedmalate transporter (ALMT) and multidrug and toxic compound extrusion (MATE), both encoding transport proteinsinvolved in Al-activated organic acid release, mainly citrate and malate. The variations in Al toxicity tolerance phenotypes arestrongly correlated with the expressions of such genes in the root apical cells. Many Al tolerance QTLs have been mapped inthe genomes of various crop species and were found to be colocated with the ALMT and MATE genes. The genetic maps ofthe Al tolerance genes and QTLs facilitate breeding programs for developing Al-tolerant cultivars through marker-assistedbreeding methods. Al tolerance genes that have been isolated from genetically unrelated species can be used in genetictransformation studies of crop genotypes sexually incompatible to the gene source genotypes. The application of thesemolecular breeding methods expedites breeding programs to develop crop cultivars tolerance to Al toxicity and acid soils.Genomic technologies by using next-generation sequencing and high-throughput genotyping system accelerate Al toxicitytolerance gene and QTL discoveries of various crop species. The modern genomic technologies also facilitate morecomprehensive PGR characterization and utilization to accelerate identification and isolation of the Al tolerance genes andQTLs to be used in a more comprehensive breeding program to support national food self sufficiency and food securityprograms.
Pengaruh Retardan Paklobutrazol terhadap Pertumbuhan dan Pemulihan Dua Aksesi Ubi Kayu Surya Diantina; Darda Efendi; Ika Mariska
Jurnal AgroBiogen Vol 11, No 3 (2015): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v11n3.2015.p95-102

Abstract

Normal growth medium is not effective for in vitro conservation due to the risk of somaclonal variation that may increase dueto short interval between subculture. Two experiments involving growth retardant paclobutrazol (PBZ) were conducted toreduce explants growth and extend subculture interval. In order to develop medium-term conservation of cassava, therecovery of plantlets after in vitro storage was also observed. Accession 433 and 450 were used in two independentexperiments. Completely Randomized Design was used with three replications. PBZ at 0, 3.4, 6.8, and 10.2 μM weresupplemented onto MS medium + arginin 100 ppm. Observation was done on shoot length, number of nodes and leaves, andnumber of white and senescence leaves. The results showed that after nine months without subculture, both cassavaaccessions showed different results in in vitro growth and their recovery. PBZ 3.4 μM performed as the best treatment inaccession 433 and 450 to reduce in vitro growth and their recovery after storage.
Penggunaan Aksis Jantung Pisang untuk Penyediaan Sumber Eksplan Bebas Bakteri Ika Roostika; Yati Supriyati; Agus Sutanto
Jurnal AgroBiogen Vol 11, No 3 (2015): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v11n3.2015.p103-110

Abstract

The sterile culture is very important in cryopreservation works. Bacterial-free explant sources are difficult to obtain during invitro culture of banana. Floral bud is expected as bacterial-free explant sources because the organ emerges above the groundand protected by bracts. The purposes of this study are to obtain optimal concentration of BA to regenerate male bud floralaxis explants of Barangan variety and to prove that cultures derived from these explants were free from bacterialcontamination. Two-millimeter pieces of male bud floral axis of Barangan variety were planted on MS medium containing of 1μM IAA, 200 g/l CH, and 2% sucrose. Experiment was arranged in Completely Randomized Design with the treatment of BA (5,10, 15, 20, and 25 μM) in 10 replications. Subculture was conducted by using MS media containing of 10 μM BA and 1 μM IAA.The variable observed were percentage of browning, number of nodules, number of shoots, number of normal shoots,number of abnormal shoots, and number of nonsurvived shoots. The screening towards bacterial contamination wasconducted by using medium containing of 10 g/l trypton, 10 g/l glucose, and 5 g/l yeast extract. The results showed that theexplants could regenerate into shoots. The 25 μM BA was the best treatment because it could produce the highest number oftotal and normal shoots, i.e. 9.2 shoots/explant and 6 shoots/explant, respectively. All of the shoots regenerated from male budfloral axis were 100% free from bacterial contamination, whereas all of the shoots regenerated from suckers werecontaminated by bacteria.
Penentuan Alergenisitas Protein Gen RB pada Kentang Produk Rekayasa Genetika Berdasarkan Studi Bioinformatika Eny Ida Riyanti; Edy Listanto; A. Dinar Ambarwati
Jurnal AgroBiogen Vol 11, No 3 (2015): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v11n3.2015.p81-86

Abstract

Genetically modified products (GMP) of Katahdin potato event SP951 containing RB gene resistant to late blight diseasescaused by Phytophtora infestans has been developed in the USA. This Katahdin SP951 potato has been crossed with localvarieties Atlantic and Granola for its development in Indonesia. In the release process, the GMP potato should be tested forenvironmental and food safety. One of the food safety assessment needs to be done by determining allergenicity of RB proteinwhether it is potential as allergen. This research aims to translate the RB gene sequence into RB protein sequence andinvestigate the potential RB protein as an allergen through bioinformatic studies. This study was performed based on thealignment with available protein allergens from available database websites. The predicted RB protein obtained from 2,913amino acids RB gene was a 971 amino acids length protein with ATG as a start codon and TAA as a stop codon. Bioinformaticsstudies of RB protein were performed using www.allergenonline.com, consisted of three searches, i.e. full-length search byFASTA, 80 amino acids search by FASTA, and 8 amino acid exact matches. For full-length alignment search, there are threeallergen proteins similar with RB protein sequence with the percentage identity of <35%, while for alignment with 80 aminoacids and 8 amino acids did not show similarity with any allergen protein in the database. It can be concluded that RB proteindid not have any potential as an allergen, as according to Codex Alimentarius guidelines for full-length alignment search, onlyprotein with identity greater than >50% indicating possible cross reactivity with protein allergen.
Deteksi dan Respons Lima Varietas Pepaya terhadap Tiga Isolat Papaya Ringspot Virus (PRSV) Tutik Harmiyati; Sri H. Hidayat; Abdul M. Adnan
Jurnal AgroBiogen Vol 11, No 3 (2015): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v11n3.2015.p87-94

Abstract

Papaya (Carica papaya L.) is one of favorite fruits for Indonesian people and known as a good vitamin source. Recently, a ringspot disease was reported in some growing areas and it potentially caused yield loss. The disease is caused by Papayaringspot virus (PRSV) and considered as a new disease in Indonesia. Therefore, preliminary research was done to study theresponse of some varieties of papaya against PRSV infection. Field isolates of PRSV were collected from Medan, Aceh, andBogor. Identification of the isolates was done based on the nucleotide sequence analysis of coat protein gene. Response ofpapaya varieties was evaluated based on the results of mechanical inoculation. Identification results showed that thenucleotide sequences of PRSV isolates Medan, Aceh, and Bogor, have a high similarity to that from other countries, i.e.Thailand, Australia, China, Japan, Vietnam, and Taiwan (93.0–98.3%). Disease incidence reached 100% for all isolates of PRSVon all test varieties, although based on the DIBA’s result, it was known that the virus titer in plants are different. No varieties ofpapaya showed resistant to PRSV.

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