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INDONESIA
Jurnal AgroBiogen
Published by Kementerian Pertanian
ISSN : 19071094     EISSN : 25491547     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Jurnal AgroBiogen memuat artikel primer dan sekunder hasil penelitian bioteknologi dan sumberdaya genetik tanaman, serangga, dan mikroba pertanian. Jurnal ini diterbitkan tiga kali setahun pada bulan April, Agustus dan Oktober oleh Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
Arjuna Subject : -
Articles 5 Documents
 1 
Search results for , issue "Vol 8, No 2 (2012): Agustus" : 5 Documents clear
Construction and Transformation of HVA1 Gene Expression Vector into Indonesian Elite Rice Varieties Sri Koerniati; Hani Widhianata
Jurnal AgroBiogen Vol 8, No 2 (2012): Agustus
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v8n2.2012.p54-61

Abstract

of drought in rice. HVA1 is one of the Late EmbryogenesisAbundant (LEA) protein group that plays a role on cellprotection during stresses. A study was done with anobjective to construct a plasmid vector expressing HVA1 andto transform it into Indonesian elite rice varieties. Materialsused in the study were plasmid pBY520 (source of HVA1;intermediate plasmid pRP9; plasmid pAY560326(backbone); restriction enzymes BamHI, HindIII, XhoI, andSpeI; T4 DNA ligase, and gel DNA extraction kit. Methodsused were standard procedure for plasmid vectorconstruction and molecular biology. Step I: the pBY520 andpRP9 were cut with BamHI and HindIII, and electrophoratedwith 1% agarose gel. DNA fragments of HVA1 and pRP9 werepurified, ligated with T4 DNA ligase, and transformed intoEscherichia coli DH5-α by heat shock. E. coli were grownonto solid medium (+ kanamycin 100 mg/l). A new plasmidDNA was isolated from single colony culture of the bacteria,confirmed, and named pRP9_HVA1. Step II: DNA ofpRP9_HVA1 and pAY560326 were cut with XhoI dan SpeIenzymes, purified, and ligated. The next procedure wassimilar to step I, and the resulted plasmid was confirmed byPCR and digestion with XhoI dan SpeI enzymes, and namedpAY_HVA1. Step III: pAY_HVA1 was first transformed intoAgrobacterium EHA-105 and then into rive varieties Ciherangand Inpari 6 using the early infection of scutellumtransformation method. Nine transgenic rice lines thatpositively contain HVA1 were obtained.
Molecular Analysis and Effectiveness Assay of AV1 Gene in Transgenic Tobacco for Resistance to Begomovirus Tri Joko Santoso; Muhammad Herman; Sri H. Hidayat; Hajrial Aswidinnoor; Sudarsono Sudarsono
Jurnal AgroBiogen Vol 8, No 2 (2012): Agustus
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v8n2.2012.p45-53

Abstract

Genetic transformationof tobacco plant using AV1 gene was conducted atthe previously experiment and generated transgenic tobaccoplants positively carrying the selectable marker nptII gene.The objectives of this experiment were to (1) analyze thepresence of Begomovirus AV1 gene in T0 generation putativetransgenic tobacco plants using PCR technique with specificprimers and its correlation with resistance phenotype, (2)analyze the integration and copy number of the transgene inT0 generation putative transgenic tobacco plants and itscorrelation with resistance response, (3) screen the T0generation putative transgenic tobacco plants with the targetvirus infection and to detect the presence of the virus in thetransgenic plant tissue using universal primers. PCRdetection of AV1 gene in tobacco transgenic was conductedby using specific primer for Begomovirus AV1 gene.Meanwhile, Southern Blot analysis was conducted by usingthe AV1 gene probe. The effectiveness of AV1 gene intobacco transgenic was tested by inoculation of target virususing whiteflies vector. Result of the experiments showedthat there was a positive correlation between the presenceof the AV1 transgene in T0 generation putative transgenictobacco plants and the resistant phenotype. Transgenicplants with a single copy integration of the transgeneexhibited more resistant than the multiple copy one. andnon transgenic plant. The resistance as a result of AV1 geneexpression was indicated with no symptom in T0 generationtransgenic tobacco plants and the accumulation of the virusin the transgenic plants tissue. Northern and Westernhybridization analysis need to be perfomed for investigatingthe presence of mRNA or protein accumulation so that theresistance mechanism of the AV1 gene could be explainedmore detail.
Pembentukan Populasi Mutan Azospirillum dengan Menggunakan Transposon untuk Sifat Superior terhadap Pelarutan P Toto Hadiarto; Ma'sumah Ma'sumah; Eny I. Riyanti
Jurnal AgroBiogen Vol 8, No 2 (2012): Agustus
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v8n2.2012.p62-68

Abstract

Azospirillum sp. which has the ability for nitrogenfixation and phosphate solubilization may support modernfarming in Indonesia that is mostly dependent on the usageof chemical fertilizer N, P, and K. Genetic quality ofAzospirillum was improved in this research to obtainsuperior characters toward phosphate solubilization so thatit can become more effective in use for farmers. To achievethis goal, Azospirillum was mutated by means ofelectroporation using transposon EZ-Tn5<kan-2>Tnp. Theelectrotransformation resulted in 20 out of 22 transformantstested contained the marker gen (npt). 10, 6 and 4 mutantshave increased, decreased and lost phosphate-solubilizingfunction, respectively. Mutant with elevated phosphatesolubilizingability may be selected further to be utilized asbiofertilizer while others may be useful for identification ofgenes responsible for phosphate solubilization.
Identifikasi dan Aplikasi Marka Berbasis PCR untuk Identifikasi Varietas Padi dengan Palatabilitas Tinggi Lestari, Puji; Risliawati, Andari; Koh, Hee Jong
Jurnal AgroBiogen Vol 8, No 2 (2012): Agustus
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v8n2.2012.p69-77

Abstract

To date, there hasbeen no DNA fingerprint profile as unique identity of ricevariety that has a high palatability (overall eating quality) inIndonesia, thus identification of premium varieties usingmolecular markers is considered to be important. This studyaimed to establish DNA fingerprint profiles of indica andjaponica rice varieties, and unique identities of rice varietieswith high palatability using molecular markers associatedwith palatability. Total of 22 japonica and 24 indica ricevarieties were evaluated their overall eating quality andtested using 20 molecular markers STS (sequence-taggedsite) which were designed on the basis of japonica ricegenome. To identify the genes functions, all these markersamplicons were cloned, transformed, sequenced and thesequences results were analyzed their homologous againstthe genome database. Ilpum (japonica) and Rojolele(indica) were identified to have the highest palatabilitycompared to other varieties. DNA fingerprint profilesidentified with the total STS markers were not able todifferentiate each variety, however premium varieties ofjaponica and indica showed specific identities. A uniqueidentity of Indonesian indica variety possessing highpalatability, Rojolele was successfully developed using amarkers set. DNA fingerprint profile in digital value systemfacilitates the identification of premium rice from othervarieties. The fragments of the STS primers showed no anyknown-genes functions related to rice eating quality,therefore these markers are preferentially used foridentification of premium rice with high palatability thandifferentiation of rice varieties based on the palatability. Inthis study, the unique identity of rice variety with highpalatability is very usefull to evaluate the purity forgermplasm protection.
Kultur Antera untuk Percepatan Perakitan Varietas Padi di Indonesia Iswari S Dewi; Bambang S. Purwoko
Jurnal AgroBiogen Vol 8, No 2 (2012): Agustus
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v8n2.2012.p78-88

Abstract

Rice is a staplefood crop in Indonesia, while the need is increasing due tohigh rice consumption as well as population increase. Theproblems can be solved through increase of national riceproduction. Productivity of lowland and upland should beincreased intensively and other potential dry area outsideJava and Bali Islands should be considered for extending thearea of production. Recently, high yielding variety such assemi dwarf variety, hybrid rice, and new plant type of ricewere being developed by Indonesian breeders. However,new method is needed to complement conventionalbreeding method in order to accelerate rice breeding.Anther culture is one of in vitro culture techniques that canbe used to accelerate the obtainment of pure lines throughdoubled-haploids (DHs) regenerated at first generation ofculture for less than one year. Thus, application of antherculture in conventional breeding will increase the efficiencyof selection process as well as reducing the cost for labour,land and breeder’s time. The obtainment of green plantletsderived from anther culture of indica rice subspecies hasbeen improved by the addition of 1 mM putrescine intoinduction and regeneration media. Recently, several uplandrice lines tolerant to abiotic stresses (i.e. low light intensityand aluminum toxicity) and biotic stresses (i.e. leaf andneck blast), several lowland rice/paddy lines tolerant tobiotic stresses (i.e. bacterial leaf blight and blast), andseveral hybrid parental lines (i.e. male sterile, maintainerand restorer) were obtained in 2-3 years from several ricebreeding program involving anther culture. However,potential use anther culture to provide unique geneticmaterial for mapping populations for use in functionalgenomics and molecular breeding has not been explored.The results indicated that anther culture is a feasibletechnology that can be used for accelerating rice breedingprogram in Indonesia.

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