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All Journal Jurnal AgroBiogen Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Pembelajaran dan Biologi Nukleus Jurnal Pengabdian Masyarakat dan Riset Pendidikan ⁠International Journal of Sustainable Social Culture, Science Technology, Management, and Law Humanities ⁠International Journal of Asian Pacific Community Service
Hani Widhianata, Hani
Universitas Kristen Cipta Wacana
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Physics Other

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 1 
Construction and Transformation of HVA1 Gene Expression Vector into Indonesian Elite Rice Varieties Sri Koerniati; Hani Widhianata
Jurnal AgroBiogen Vol 8, No 2 (2012): Agustus
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v8n2.2012.p54-61

Abstract

of drought in rice. HVA1 is one of the Late EmbryogenesisAbundant (LEA) protein group that plays a role on cellprotection during stresses. A study was done with anobjective to construct a plasmid vector expressing HVA1 andto transform it into Indonesian elite rice varieties. Materialsused in the study were plasmid pBY520 (source of HVA1;intermediate plasmid pRP9; plasmid pAY560326(backbone); restriction enzymes BamHI, HindIII, XhoI, andSpeI; T4 DNA ligase, and gel DNA extraction kit. Methodsused were standard procedure for plasmid vectorconstruction and molecular biology. Step I: the pBY520 andpRP9 were cut with BamHI and HindIII, and electrophoratedwith 1% agarose gel. DNA fragments of HVA1 and pRP9 werepurified, ligated with T4 DNA ligase, and transformed intoEscherichia coli DH5-α by heat shock. E. coli were grownonto solid medium (+ kanamycin 100 mg/l). A new plasmidDNA was isolated from single colony culture of the bacteria,confirmed, and named pRP9_HVA1. Step II: DNA ofpRP9_HVA1 and pAY560326 were cut with XhoI dan SpeIenzymes, purified, and ligated. The next procedure wassimilar to step I, and the resulted plasmid was confirmed byPCR and digestion with XhoI dan SpeI enzymes, and namedpAY_HVA1. Step III: pAY_HVA1 was first transformed intoAgrobacterium EHA-105 and then into rive varieties Ciherangand Inpari 6 using the early infection of scutellumtransformation method. Nine transgenic rice lines thatpositively contain HVA1 were obtained.
Active Sites Determination of Enzyme 1-aminocyclopropane-1-carboxylic acid synthase 2 (ACS2) of Capsicum chinense using Modeling and In Silico Docking Pahlevi, Muhammad Rizza; Widhianata, Hani; Puspaningsih, Eunike Sri; Lestari, Juniarti Wulan; Sudibya, Eka; Suwasono, Prayitno Ribut; Habiburrahman , Novan
⁠International Journal of Sustainable Social Culture, Science Technology, Management, and Law Humanities Vol. 1 No. 2 (2024)
Publisher : Universitas Kristen Cipta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.71131/d1yzr175

Abstract

The growth of Chili pepper (Capsicum chinense Jacquin.) is affected by biotic and abiotic stresses. Abiotic stress such as waterlogging increases the expression of ACS (1-aminocyclopropane-1-carboxylic acid synthase) enzyme. This enzyme plays a great role in the process of ethylene biosynthesis and encoded by multiple genes. Waterlogging causes hypoxia condition. One of ACS enzymes that responds to hypoxia condition is ACS2. The different respond to hypoxia stress among plant was assumed to be caused by a different structure of the ACS2 enzyme. This study aimed to identify and confirm the active site of Capsicum chinense Jacquin ACS2  using modeling and in silico docking. The result of the three-dimensional (3D) structure modeling showed 91% similarity of Capsicum chinense Jacquin ACS2 with the structure of the tomato (Solanum lycopersicum L.). The Capsicum chinense ACS2 confirmed five active sites that bind to the substrates asparagin396B, valine397B, thyrosine152B, threonine128B, and thyrosine92A. 
Development of environmentally conscious animal food supply to meet protein consumption in Blitar district Azis, Rosidi; Juniarti Wulan Lestari; Hani Widhianata; Eunike Sri Puspaningsih; Eka Sudibya; Novan Noviansah; Triawati, Indri
⁠International Journal of Asia Pacific Community Service Vol. 1 No. 2 (2024)
Publisher : Universitas Kristen Cipta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.71131/5rmrmf64

Abstract

Developing environmentally friendly livestock food provision in Blitar Regency through duck farming is one of the strategic efforts to increase community animal protein consumption while maintaining environmental sustainability. This community service program is designed with a participatory approach, education, and application of appropriate technology. The methods used include surveys, farmer training, implementation of technology to increase livestock productivity, use of biodigesters, and strengthening farmer institutions. The evaluation results showed a significant increase in farmer knowledge and skills, with an average post-test score increasing by 40% compared to the pre-test. The adoption of appropriate technology, such as feed biofermentation, and biodigesters, has succeeded in reducing production costs by 30%. Livestock productivity increased with production, while duck mortality rates decreased from 15% to 5%. Strengthening farmer institutions through the formation of farmer groups also increases the organizational capacity and bargaining power of farmers, including in accessing government financing and support. This program not only has a positive impact on farmer welfare but also contributes to meeting the community's animal protein needs and better environmental management. The sustainability of this program is expected to be achieved through supervision and synergy between the government, academics, and the private sector. This holistic approach shows great potential for replication in other areas with similar conditions.
Morphological Characterization of Tea Hybrid Progeny Widhianata, Hani; Noviansyah, Novan; Sudibya, Eka; Suwasono, Prayitno Ribut
⁠International Journal of Sustainable Social Culture, Science Technology, Management, and Law Humanities Vol. 2 No. 1 (2025)
Publisher : Universitas Kristen Cipta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.71131/dpp2hp13

Abstract

Hybridization is the main method for obtaining genetic diversity and breeding new varieties of tea. The purpose of this research is to determine the morphological potential of each progeny from crossing between male and female parents which can then be used as a source of information in developing high quality superior tea clones through tea breeding activities. Male and female parents with the desired traits were crossed to form genetic diversity. 34 progenies resulted from crosses formed from crossing 4 male parents and 3 female parents. All parents and progeny were characterized morphologically and yield components. Data on morphological characters and yield components were analyzed descriptively. Correlation analysis and Principles Component Analysis Biplot (PCA Biplot) were also performed using R software. Male tea parents and female tea parents are in different groups based on morphological characteristics. Progeny-17, Progeny-18, Progeny-4, Progeny-5, and Progeny-27 had a higher total number of shoot and potential yield per bush compared to the other progenies. 
Penguatan Peran Kelompok Swadaya Masyarakat dalam Pengelolaan Sampah Domestik di Kawasan Permukiman: Pengabdian Aria Dian Tri Wahyuni; Arinda Eka Lidiastuti; Khalilah Daud Isaac Makhmut; Hani Widhianata; Ika Wardani; Arini Zulfaida
Jurnal Pengabdian Masyarakat dan Riset Pendidikan Vol. 3 No. 4 (2025): Jurnal Pengabdian Masyarakat dan Riset Pendidikan Volume 3 Nomor 4 (April 2025
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jerkin.v3i4.1060

Abstract

The issue of domestic waste management in Indonesia remains a significant challenge, especially at the household level in residential areas. Despite existing regulations and community-based waste management efforts, implementation in the field still faces various obstacles such as a lack of awareness and institutional support. This study conducted a community service intervention by strengthening the role of the Community Self-Help Group (Kelompok Swadaya Masyarakat, KSM) in RW 01, Rampal Claket Village, Malang City, through training, technical facilitation, and the development of a community-based waste management model using a Participatory Action Research (PAR) approach. The results show a significant increase in residents’ participation in sorting, managing, and recycling waste, as well as the strengthening of KSM’s organizational capacity in operational management and waste-based entrepreneurship. This program contributed to the reduction of inorganic waste volume sent to the Final Disposal Site (Tempat Pembuangan Akhir, TPA), empowered the community’s micro-economy, and enhanced environmental awareness. These findings emphasize the importance of triple helix collaboration among universities, government, and communities in building an effective, sustainable waste management system with positive socio-ecological impacts.
Characterization and Formulation of Butterfly Pea Flower Drinks (Clitoria ternatea L.) Fermented by Pineapple Peel based on Organoleptic Attributes and Gen-Z Acceptance Pahlevi, Muhammad Rizza; Widhianata, Hani; Kurniahu, Hesti
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 3: Jurnal Pembelajaran Dan Biologi Nukleus September 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i3.7907

Abstract

Background: Butterfly pea flowers (BPF) Clitoria ternatea were known as an antioxidant-rich functional drink ingredient at approximately 6.58 ppm. Various kinds of BPF drinks formulations had been consumed by the public, especially the older generation, due to their benefits. Meanwhile, the younger generation (Generation Z) was not familiar with these drinks and tended to enjoy soft drinks with sweet and fizzy flavors. This study formulated BPF with fermented pineapple peel (FPP) as an alternative to soft drinks. Methodology: Based on the SNI 01-2346-2006 standard, a total of 34 untrained Generation Z panelists were used to test organoleptic attributes (color, flavor, aroma, and sensation) using the Check-All-That-Apply approach and the hedonic test 5-point scale approach for panelist acceptability. Five formulations were used: BPF without sugar (A), with 10% sugar (B), (FPP + 10% sugar) (C), (BPF + FPP + 10% sugar) (D), and (BPF + FPP + 20% sugar) (E). Results: Based on Kruskal-Wallis with the Mann-Whitney further test for all organoleptic attributes, there were significant differences; the preferred color was formulation A, the aroma acceptability was neutral for all formulations, the preferred flavor was formulation E with a strong sweet and sour flavor, and for the sensation, formulation A was not preferred. The overall rating assessment showed that formulation E had the highest score due to strong sweet-sour flavor. Formulation E became the  favorite drink of the panelist. This formulation could be a consideration for functional BPF drink innovation, which is the novelty of this study as an alternative to soft drinks with the Generation Z market share. Contribution: This study contributes scientifically by demonstrating that the combination of antioxidant-rich butterfly pea flower and fermented pineapple peel can be formulated into a functional drink with sensory attributes acceptable to Generation Z, thus bridging traditional herbal drinks with modern consumer preferences
Efektivitas Rempah dan Herbal sebagai Antioksidan Alami dalam Pengawetan Ikan Tuna (Thunnus sp.) dan Ikan Nila (Oreochromis niloticus): Meningkatkan Keamanan Mikroba dan Daya Simpan Widhianata, Hani; Lidiastuti, Arinda Eka
Jurnal Penelitian Pendidikan IPA Vol 11 No 11 (2025): November: In Progress
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i11.12802

Abstract

Fish is a protein-rich and highly nutritious food. Natural herbs and spices contain antioxidant and antibacterial compounds that may enhance microbiological safety and extend shelf life. This study aimed to evaluate natural herb and spice-based processing methods for preserving tuna (Thunnus sp.) and nile tilapia (Oreochromis niloticus), along with the effectiveness of specific spices in reducing oxidation and microbiological contamination. Two marination approaches were applied, using both uncooked and cooked spice mixtures. Three recipes were prepared, recipe 1: garlic, pepper, salt, turmeric, coriander, lemongrass, recipe 2: garlic, pepper, salt, rosemary, oregano, thyme, lemon and recipe 3: garlic, pepper, salt, coriander, lemongrass, chili pepper, curry leaves, asam sunti. Unmarinated fish served as the control. All samples were vacuum-packed in retort pouches and stored at 1-4 °C for 1 h, 3, 5, and 7 days. Microbial analysis included total aerobic bacteria, Escherichia coli, Coliforms, Salmonella spp., Shigella spp., yeast and mold. Marination with herbs and spices significantly reduced microbial contamination compared to the control. The combined use of herbs and spices effectively improved microbial safety and extended the shelf life of tuna and nile tilapia. These findings support the application of herbs and spices as natural preservation agents in fish products, offering a safer and more sustainable alternative in fish preservation.
Co-Authors Aria Dian Tri Wahyuni Arini Zulfaida Azis, Rosidi Eka Sudibya Eunike Sri Puspaningsih Ika Wardani Juniarti Wulan Lestari Kurniahu, Hesti Lestari, Juniarti Wulan Lidiastuti, Arinda Eka Makhmut, Khalilah Daud Isaac Novan Habiburrahman Novan Noviansah Noviansyah, Novan Pahlevi, Muhammad Rizza Puspaningsih, Eunike Sri Sri Koerniati Sudibya, Eka Suwasono, Prayitno Ribut Triawati, Indri