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Buletin Plasma Nutfah
Published by Kementerian Pertanian
ISSN : 14104377     EISSN : 25491393     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Buletin Plasma Nutfah (BPN) is an open access scientific journal published by The Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD), The Indonesian Agency for Agricultural Research and Development (IAARD), Ministry of Agriculture. This peer-refereed journal covering the area of genetic resources including exploration, characterization, evaluation, conservation, diversity, traditional knowledge, management and policy / regulation, of all kinds of genetic resources: plants, animals, fishes, insects and microbes. Manuscripts submitted to this journal are those that have never been published in other journals. This journal is published in one volume of two issues per year (June and December). We invite authors to submit the manuscripts to this journal in English or Indonesian. Detail information about the journal, including author guidelines and manuscript template, is available on the website (http://ejurnal.litbang.pertanian.go.id/index.php/bpn). The manuscript should be submitted electronically through our submission system. Buletin Plasma Nutfah (BPN) is an open access scientific journal published by Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD), Indonesian Agency for Agricultural Research and Development (IAARD), Ministry of Agriculture. BPN Accredited by Ministry of Research, Technology and Higher Education of the Republic of Indonesia (No. 21/E/KPTP/2018) for period of 2016-2020. This peer-refereed journal covers the area of genetic resources including exploration, characterization, evaluation, conservation, diversity, traditional knowledge, management and policy / regulation related to genetic resources: plants, animals, fishes, insects and microbes which has never been published in other Journal
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Articles 7 Documents
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Search results for , issue "Vol 12, No 1 (2006)" : 7 Documents clear
Karakterisasi Plasma Nutfah Kangkung Yenni Kusandriyani; nFN Luthfy
Buletin Plasma Nutfah Vol 12, No 1 (2006)
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v12n1.2006.p30-33

Abstract

Characterization of Ipomoea reptans germplasm collection were done in order to describe the accessions and to utilize as working collection materials for breeding program. Description was aimed to identify the new collections of 15 accessions I. reptans which were taken from IVEGRI (Indonesia Vegetable Goverment Research Institute) germplasm collection. The experiment was carried out in Subang station (100 m asl) in dry season 2002. Each accession were planted 40 plant per plot of I. reptans with plant distance 20 x 30 cm. The plants of were fertilized with stable manure 20 t/ha and NPK (15 : 15 : 15) 500 kg/ha. The result of the study was a description of genetic resources that will be exchanged and utilized in breeding for high yielding varieties of I. reptans with better quality. AbstrakKarakterisasi plasma nutfah kangkung dilakukan untuk mendapatkan deskripsi sifat tanaman dan sebagai bahan working collection yang akan digunakan sebagai bahan dalam program pemuliaan. Penelitian mencakup 15 nomor aksesi kangkung di Kebun Percobaan Subang (100 m dpl) Jawa Barat pada musim kemarau 2002, tiap nomor dari masing-masing aksesi ditanam sebanyak 40 tanaman. Tanaman diberi pupuk kandang domba dengan takaran 20 t/ha dan NPK (15 : 15 : 15) 500 kg/ha. Hasil penelitian ini menghasilkan deskripsi plasma nutfah tanaman kangkung untuk pertukaran informasi mengenai sumber genetik yang akan digunakan dalam pemuliaan untuk mendapatkan varietas unggul dengan kualitas yang lebih baik.
Karakterisasi Morfologi dan Mutu Buah Mengkudu Endjo Djauhariya; Mono Rahardjo; nFN Ma'mun
Buletin Plasma Nutfah Vol 12, No 1 (2006)
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v12n1.2006.p1-8

Abstract

The characterization of indian mulberry or noni (Morinda citrifolia L.) was conducted in some areas of Java in May to December 2003 to study their morfological and quality variations. It was found that there were seven distinct groups of noni types. Base on fruit size, there were two distinguished groups; types with big fruits (Types 1, 2, 4, and 5), and types with small fruits (types 3, 6, and 7). Big fruit had higher extract concentration than small fruit. Type 4 was seed less, had in highest juice percentage. The seed less type was rare and only found in Surakartya and Semarang in a limited population. The types widely grown throughout the entire survey areas. The type 5 has highest fruit weight, its fruits grew in cluster, it was found in Malingping and Tasikmalaya. Base on fruits taste, none could be grouped into two groups: fruits with sour-sweet taste and fruit with bitter taste. All types which grew near the beach had bitter taste. The bitter fruits did not fall when they were ripe, whereas the sour-sweet fruits fall at the some of ripening. AbstrakPenelitian dilakukan di sentra pertanaman mengkudu di Jawa Barat (Ciampea, Cipaku, Jasinga, Tasikmalaya), Banten (tepi pantai Malingping), Jawa Tengah (Surakarta, Semarang, Yogyakarta), dan di Jawa Timur (Lamongan) pada bulan Mei- Desember 2003, untuk mengetahui ciri-ciri morfologi dan mutu buah mengkudu. Telah diperoleh tujuh tipe mengkudu dengan perbedaan sifat-sifat morfologi, fisiologi, dan mutu buahnya. Perbedaan tipe mengkudu yang mencolok adalah bentuk, ukuran, rasa, dan rendemen daging buah. Dari ketujuh tipe tersebut terdapat dua tipe yang berukuran buah besar (tipe 1, 2, 4, dan 5), dan tipe berukuran buah kecil (tipe 3, 6, dan 7). Tipe mengkudu yang ukuran buahnya besar, rendemen jus dan daging buahnya lebih tinggi dibandingkan dengan yang buahnya berukuran kecil. Dari tipe berukuran buah besar terdapat satu tipe berbiji sedikit yang dianggap seed less, yaitu tipe 4, sehingga rendemen daging buahnya tinggi. Tipe seed less termasuk langka, karena hanya ditemukan di Surakarta dan Semarang dengan populasi terbatas. Tipe yang mempunyai daerah penyebaran luas adalah tipe 1, ditemukan di tepi pantai hingga ketinggian 600 m dpl. Tipe 5 bobot buahnya paling tinggi, buah bercabang, ditemukan di Malingping dan Tasikmalaya. Rasa daging buah, dapat dibedakan menjadi dua kelompok, yaitu asam manis dan pahit. Semua tipe yang tumbuh di tepi pantai rasa buahnya pahit, dan setelah masak buahnya tidak langsung jatuh. Kelompok yang mempunyai rasa buah tidak pahit, buah tidak langsung jatuh setelah masak.
Kuantitas dan Kualitas Nutrisi Pakan Orangutan di Pusat Reintroduksi Orangutan, Wanariset Samboja-Kalimantan Timur nFN Zuraida
Buletin Plasma Nutfah Vol 12, No 1 (2006)
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v12n1.2006.p34-39

Abstract

Orangutan (Pongo pygmaeus) was an endangered species with a decreasing population gradually. To overcome the problem, reintroduction was a good conservation program. This was done through a rehabilitation that aim was ad at increasing the adaptability of orangutan which were adapted to human environment, so that they can survive in their natural habitat. The nutrient adequacy for orangutans while staying in the reintroduction center was very important before they were released into natural habitat. Therefore, the objectives of this study were to find out the kinds of daily feed consumption, nutrient contents and nutrient intake of orangutans at Wanariset Samboja by measuring the average daily nutrient intake of individual orangutans. Data were obtained from eight samples of orangutans in individual cages containing a male and a female at the age of two year old, and three males and three females at the age of four year old. The data of feed consumption were recorded three times daily, namely, morning, noon, evening in terms of feed amount and types, then converted into the nutrient values based on table of nutrient contents listed in standardized table. The differences in nutrient intake based on sex and age were obtained by Duncan analysis. The results of the study showed that the daily nutrient intake of 8 orangutans during 24 hours more than energy expenditure in the cage. Total energy used for 24 hours by female and male orangutans at the age of two years old were 692.57 and 739.60 calories, respectively, whereas the energy used by orangutans at the age of four years old were 1088.84 and 945.57 calories, respectively. Calories consumed from feed at the age of two years for female and male were 1042,10 and 1066,60 calories respectively while at the age of four years for female and male were 1402,03 calories and 1227,40 calories respectively. According to the above data energy consumed from feed was more than energy expenditure. It was concluded that feeding consumption given at Wanariset samboja was adequate. AbstrakOrangutan (Pongo pygmaeus) adalah salah satu jenis satwa langka yang populasinya semakin menurun. Untuk mengatasi masalah ini dilakukan upaya pengembalian orangutan ke habitat aslinya dengan sistem rehabilitasi. Dalam hal ini ketersediaan pakan merupakan hal yang penting. Penelitian ini bertujuan untuk mengetahui informasi penyediaan pakan, kandungan, dan asupan nutrisi harian orangutan di Wanariset Samboja. Data yang diambil berasal dari delapan sampel orangutan di kandang individu yang terdiri atas satu ekor jantan dan satu ekor betina umur 2 tahun, serta tiga ekor jantan dan tiga ekor betina umur 4 tahun. Jumlah dan jenis pakan yang dikonsumsi dikonversi ke nilai kandungan nutrisi yang terdapat dalam tabel kandungan bahan makanan. Perbedaan asupan nutrisi berdasarkan jenis kelamin dan umur diketahui dengan uji Duncan. Hasil penelitian menunjukkan bahwa asupan kalori harian delapan orangutan contoh selama 24 jam lebih banyak dibandingkan dengan kalori yang dikeluarkan orangutan selama di kandang. Total energi yang dikeluarkan selama 24 jam oleh orangutan betina dan jantan berumur 2 tahun berturutturut adalah 692,57 dan 739,60 kalori, sedangkan untuk umur 4 tahun adalah 1088,84 dan 945,61 kalori. Kalori yang didapat dari makanan untuk orangutan betina dan jantan umur 2 tahun berturut-turut adalah 1042,10 dan 1066,60 kalori dan untuk orangutan yang berumur 4 tahun berturut-turut adalah 1402,03 dan 1227,40 kalori. Dengan demikian, jumlah energi yang didapat dari makanan lebih besar dibandingkan dengan energi yang dikeluarkan. Berdasarkan data tersebut dapat disimpulkan jumlah pakan yang diberikan di pusat rehabilitasi orangutan mencukupi kebutuhan.
Status Penelitian Purwoceng (Pimpinella alpina Molk.) di Indonesia Ireng Darwati; Ika Roostika
Buletin Plasma Nutfah Vol 12, No 1 (2006)
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v12n1.2006.p9-15

Abstract

Purwoceng was a commercial medicinal plant that could be used as aphrodisiac, diuretic, and body fit enhancer. The plant was indigenous of Indonesia that grew endemically at Dieng Plateau in Central Java, Pangrango Mountain in West Java, and mountaineos area in East Java. Recently the population was getting rare because of high genetic erosion. Based on the erosion level, the purwoceng was categorized as endangered species. In order to prevent from extinction, the conservation has to be done. The efforts of conservation could be conducted together with the efforts of its utilization optimally and sustainably. So far there were not many researches on purwoceng. Several aspects that had been reported were on agronomy, in vitro culture, phytochemistry, and pharmacology. However, the results of those researches had not been optimal and satisfying. Breeding research had not even been reported. This condition opened large opportunities for researchers to develop the researches that had been conducted to obtain the new technology. The supported technologies and the completed information would enhance the development of this commodity especially at industrial scale. Abstrakdigunakan sebagai afrodisiak, diuretic, dan tonik. Tanaman tersebut adalah tumbuhan asli Indonesia yang tumbuh secara endemik di dataran tinggi Dieng Jawa Tengah, Gunung Pangrango Jawa Barat, dan area pegunungan di Jawa Timur. Dewasa ini, populasinya sangat jarang yang disebabkan oleh erosi genetik secara besar-besaran. Berdasarkan tingkat erosinya, purwoceng dikategorikan sebagai spesies yang hampir punah. Untuk menghindari kepunahan, tindakan konservasi harus dikelola dengan baik. Upaya pelestarian sebaiknya dilakukan secara bersama dengan upaya pemanfaatannya secara optimal dan berkelanjutan. Hingga saat ini tidak banyak laporan penelitian tentang purwoceng. Beberapa aspek yang sudah dilaporkan adalah aspek agronomi, kultur in vitro, fitokimia, dan farmakologi. Namun demikian, hasil penelitian tersebut belum memuaskan. Penelitian pemuliaan bahkan belum pernah dilaporkan. Kondisi demikian membuka peluang bagi pengembangan penelitian yang sudah pernah dilakukan hingga diperoleh teknologi yang mantap. Teknologi yang mendukung dan informasi yang lengkap diharapkan akan meningkatkan pengembangan komoditas tersebut, terutama dalam skala industri.
Pengaruh Proses Freeze-Drying dan Penyimpanan pada Suhu Kamar terhadap Viabilitas dan Patogenisitas Plasma Nutfah Mikroba Pasteurella Multocida Siti Chotiah
Buletin Plasma Nutfah Vol 12, No 1 (2006)
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v12n1.2006.p40-44

Abstract

The effect of freeze-drying process and preserving in a vacuum at room temperature against viability and pathogenicity of veterinary microbe germ plasma of Pasteuerella multocida BCC 2331 was investigated at Balitvet. The aim of this study was to find out the most effective and efficient conservation method. As much as 5,2 x 1011 colony forming unit (CFU)/ml of bacteria suspension in 7.5% glucose serum as the preservation medium being pathogenic in mice with LD50 of 9,8 CFU/ml was freeze dried then stored at room temperature (±27oC) until the study was completed. Viability and pathogenicity test were done immediately after the process, 1 and 2 months after storage. The results showed that there were viability decreases amounted 1,3 x 101 CFU/ml, 102 CFU/ml and 8,2 x l02 CFU/m1 due to the effects of the process, one month and two-month storage respectively. The decreases of pathogenicity on mice were shown by the increases of LD50 amounting log 1, log 2, and log 3 a day after the process, one month and two-month storage respectively. AbstrakPengaruh proses kering beku dan penyimpanan hasil proses pada suhu kamar 27oC terhadap viabilitas dan patogenisitas plasma nutfah mikroba veteriner telah dipelajari di Balitvet untuk menentukan cara pelestarian yang efektif dan efisien. Dalam kegiatan ini dipakai bakteri Pasteurella multocida koleksi Balitvet Culture Collection nomor koleksi B2331. Suspensi bakteri sebanyak 5,2 x l011 coloni forming unit (CFU)/ml dalam medium preservan 7,5% glukosa, serum dan bersifat patogen pada mencit dengan LD50 9,8 CFU/ml diproses kering beku, kemudian disimpan pada suhu kamar (+27oC) sampai penelitian selesai. Uji viabilitas dan patogenisitas dilakukan langsung setelah proses dan pada 1 serta 2 bulan setelah penyimpanan. Hasil penelitian menunjukkan terjadi penurunan viabilitas sebanyak 1,3 x 101 CFU, dan 8,2 x 102 CFU/ml masingmasing karena pengaruh proses, pengaruh penyimpanan selama 1 dan 2 bulan. Patogenisitas pada mencit menurun yang ditandai oleh adanya peningkatan LD50 sebanyak log 1, log 2, dan log 3 masing-masing 1 hari setelah proses, 1 dan 2 bulan setelah penyimpanan.
Pengelolaan Sumber Daya Genetik Tanaman Obat Spesifik Kalimantan Tengah Amik Krismawati; M. Sabran
Buletin Plasma Nutfah Vol 12, No 1 (2006)
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v12n1.2006.p16-23

Abstract

Central Kalimantan Province covers an area of 15,38 million ha which consist of 61,140 ha coastal region, 1,53 million ha open waters, and 13,79 million ha as inland area. The continental area consisted of various type of lands e.g. acid sulphate, peats soil, and up land. In order to explore and conserve potential medicinal plants indigenous to Central Kalimantan, these exploration and characterization activities were conducted. Method of study: (1) exploration, (2) ex situ conservation, (3) characterization, and (4) documentation. The result of these activities are ex situ collection of 15 acessions of medicinal crops. This exploration obtained 15 accessions of medicinal plants which were characterized and conserved in ex situ condition. AbstrakKalimantan Tengah memiliki luas wilayah 15,38 juta ha yang terdiri dari 61,1 ribu ha daerah pantai, 1,53 juta ha daerah perairan umum, dan 17,79 juta ha daratan. Wilayah daratan yang luas ini terdiri atas berbagai tipologi lahan seperti lahan sulfat masam, gambut, dan lahan kering. Untuk menggali potensi dan melestarikan plasma nutfah tanaman obat spesifik Kalimantan Tengah telah dilaksanakan kegiatan eksplorasi dan karakterisasi. Dari kegiatan ini dapat disusun deskripsi berbagai jenis tanaman tersebut untuk inventarisasi, karakterisasi, dan koleksi tanaman obat secara ex situ dalam bentuk kebun pembibitan. Metode kegiatan meliputi (1) eksplorasi, (2) konservasi ex situ, (3) karakterisasi, dan (4) dokumentasi. Hasil kegiatan adalah koleksi secara ex situ tanaman obat sebanyak 15 aksesi.
Ekologi dan Potensi Ramin (Gonystylus bancanus Kurz.) di Kelompok Hutan Sungai Tuan-Sungai Suruk, Kalimantan Barat N. M. Heriyanto; R. Garsetiasih
Buletin Plasma Nutfah Vol 12, No 1 (2006)
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v12n1.2006.p24-29

Abstract

Ecological and potential study of ramin was conducted in September 2002. Sampling plot of 20 meters x 1,000 meters with distance between plots was 1 km with total transect of three. Observation within the transect were on stage of tree, pole, sapling and seedling measured. Predominant species were Gluta renghas, Kingiodendron sp., and Shorea spp. The tree stages of ramin was 1.48 tree/ha, poles stage 33.75 individual/ ha, sapling stage 125 individual/ha, and seedling stage 468.75 individual/ha. Ramin was closely related to Kingiodendron sp., G. renghas, and Mezzettia parviflora. However, this species did not associate with Litsea sp., Dryobalanops abnormis, and Canarium caudatum. The habitat of ramin was peat swamp forest with the depth of more than 3 m and influenced by tide but the water remain unsalted. AbstrakUntuk mengetahui ekologi dan potensi jenis ramin (G. bancanus) di habitat alamnya dilakukan penelitian pada bulan September 2002. Penelitian menggunakan satuan contoh berupa jalur dengan lebar 20 m, panjang 1.000 m dengan jumlah pengamatan 3 jalur dan jarak antar jalur 1 km. Pada jalur ini dilakukan pengukuran semai, pancang, tiang, dan pohon. Jenis tumbuhan yang mendominasi tegakan di kelompok hutan Sungai Tuan-Sungai Suruk adalah Gluta renghas (rengas), Kingiodendron sp. (sempetir), dan Shorea spp. (meranti). Ramin memiliki kelimpahan 1,48 pohon/ha untuk tingkat pohon, 33,75 individu/ha untuk tingkat tiang, 125 individu/ha untuk tingkat pancang, dan 468,75 individu/ha untuk tingkat semai. Jenis-jenis yang berasosiasi kuat dengan ramin adalah Kingiodendron sp. (sempetir), Gluta renghas (rengas), dan Mezzettia parviflora (pisang-pisang). Sedangkan jenis yang berasosiasi tidak kuat dengan ramin adalah Litsea sp. (medang), Dryobalanops abnormis (kelansau), dan Canarium caudatum (kenari hutan). Habitat ramin adalah hutan rawa gambut yang umumnya mempunyai kedalaman lebih dari 3 m yang terpengaruh oleh pasang surut tetapi airnya tidak pernah asin.

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