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Buletin Plasma Nutfah
Published by Kementerian Pertanian
ISSN : 14104377     EISSN : 25491393     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Buletin Plasma Nutfah (BPN) is an open access scientific journal published by The Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD), The Indonesian Agency for Agricultural Research and Development (IAARD), Ministry of Agriculture. This peer-refereed journal covering the area of genetic resources including exploration, characterization, evaluation, conservation, diversity, traditional knowledge, management and policy / regulation, of all kinds of genetic resources: plants, animals, fishes, insects and microbes. Manuscripts submitted to this journal are those that have never been published in other journals. This journal is published in one volume of two issues per year (June and December). We invite authors to submit the manuscripts to this journal in English or Indonesian. Detail information about the journal, including author guidelines and manuscript template, is available on the website (http://ejurnal.litbang.pertanian.go.id/index.php/bpn). The manuscript should be submitted electronically through our submission system. Buletin Plasma Nutfah (BPN) is an open access scientific journal published by Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD), Indonesian Agency for Agricultural Research and Development (IAARD), Ministry of Agriculture. BPN Accredited by Ministry of Research, Technology and Higher Education of the Republic of Indonesia (No. 21/E/KPTP/2018) for period of 2016-2020. This peer-refereed journal covers the area of genetic resources including exploration, characterization, evaluation, conservation, diversity, traditional knowledge, management and policy / regulation related to genetic resources: plants, animals, fishes, insects and microbes which has never been published in other Journal
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Articles 6 Documents
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Search results for , issue "Vol 18, No 2 (2012): December" : 6 Documents clear
Ketahanan Plasma Nutfah Kapas terhadap Hama Pengisap Daun, Amrasca biguttula (ISHIDA) IGAA. Indrayani; Siwi Sumartini
Buletin Plasma Nutfah Vol 18, No 2 (2012): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v18n2.2012.p77-83

Abstract

Resistance of Cotton Germplasms Against Sucking Insect Pest, Amrasca biguttula (ISHIDA). IGAA. Indrayani and Siwi Sumartini. Morphological characteristics of cotton leaf have an important role on the resistance against sucking insect pest. Among the characters, leaf hair density is the most important in preventing the attack of sucking insect and it can be used to identify the resistance of cotton germplasms against sucking insect pest, A. biguttula. Study on resistance of cotton germplasms against sucking insect pest, A. biguttula (Ishida) was carried out at Asembagus Experimental Station of Indonesian Tobacco and Fiber Crops Research Institute (IToFCRI) in Malang from January to December 2009. The objective of study was to find out resistant cotton germplasms to A. biguttula. Fifty accessions of cotton germplasm were used as treatment and arranged in Randomized Block Design (RBD) with three replications. Plot size used was 10 m x 3 m that consists of two rows of tested accession and one row of Tamcot SP 37 as an atractant plant for A. biguttula. Parameters observed were leaf hair density, length of hair, population of A. biguttula nymph, and plant damage. Cotton accessions with higher hair density and length of leaf hair significantly reduced the frequency of action threshold population of A. biguttula and plant damage. Eleven cotton accessions, viz., SATU 65; VAR 78443; Sukothai 14; GM5U/4/2; Samir 730; L1; L4 x Rex/1; Paymaster 404; ISA 205B; Albar 72B; dan Tashkent 2 were showed more resistant to A. biguttula because of higher leaf hair density (121-360 hairs/cm2), lower nymph population (0-2 times of population threshold) and lower damage score (1.0-1.8). These cotton accessions could be promising to be genetic resources of resistance to sucking insect pest, A. biguttula. AbstrakKarakteristik morfologi daun kapas mempunyai peran penting pada ketahanan terhadap hama pengisap. Di antara sifat morfologi tersebut, kerapatan bulu daun sangat berperan dalam menghambat serangan pengisap sehingga sifat ini dapat digunakan untuk mengidentifikasi aksesi kapas yang tahan A. biguttula. Penelitian ketahanan aksesi kapas terhadap hama pengisap, A. biguttula dilakukan di KP. Asembagus Balai Penelitian Tanaman Tembakau dan Serat mulai Januari hingga Desember 2009. Penelitian ini bertujuan untuk mendapatkan aksesi kapas tahan A. biguttula. Sebanyak 50 aksesi kapas digunakaan sebagai perlakuan yang masing-masing disusun dalam Rancangan Acak Kelompok (RAK) dengan tiga kali ulangan. Ukuran petak adalah 10 m x 3 m yang terdiri atas 2 baris aksesi yang diuji dan 1 baris Tamcot SP 37 sebagai tanaman penarik A. biguttula. Parameter yang diamati adalah kerapatan (jumlah) bulu daun, panjang bulu daun, populasi nimfa A. biguttula, dan skor kerusakan tanaman. Aksesi dengan kerapatan bulu daun yang tinggi dan berbulu panjang secara nyata menurunkan frekuensi pencapaian populasi ambang kendali dan kerusakan tanaman. Sebelas aksesi kapas dengan jumlah bulu berkisar 121-360 helai/cm2, populasi nimfa rendah (frekuensi ambang rendah, 0-2 kali) dan skor kerusakan rendah (1,0-1,8) adalah SATU 65; VAR 78443; Sukothai 14; GM5U/4/2; Samir 730; L1; L4 x Rex/1; Paymaster 404; ISA 205B; Albar 72B; dan Tashkent 2. Aksesi ini berpotensi sebagai materi genetik untuk ketahanan terhadap A. biguttula.
Seleksi Beberapa Klon Kentang (Solanum tuberosum L.) dari Hasil Persilangan untuk Karakter Daya Hasil Tinggi pada Ekosistem Dataran Tinggi di Ciwidey nFN Kusmana
Buletin Plasma Nutfah Vol 18, No 2 (2012): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v18n2.2012.p45-53

Abstract

Selection of Several Potato Clones (Solanum tuberosum L.) Derived from Crosses for High Yielding on Highland of Ciwidey. Kusmana. Granola and Atlantic are two dominant varieties grown by farmers. So far no other alternative superior varieties that can be used by farmers to develop potato cultivation in the field. The objective of the research was to identify potato clones that were highest yielding in the highland Ciwidey and the potential for multy location evaluation. The experiment was conducted in Ciwidey, Bandung District at altitude 1.400 m above sea level. A total of 41 advanced breeding materials and variety was planted. A Randomized Complete Block Design with three replications was used and an experimental unit consisted of 10 hill/plot. The result indicated that high yielding clones (up to 35.6 t/ha) were obtained clones 29, (43.8 t/ha), 11 (38.2 t/ha), 31 (37.3 t/ha), 13 (36 t/ha) and 35 (35.6 t/ha). Whereas, the variety of Granola as a comparison was yielded only 23.4 t/ha. Clones 9, 22 and 35 were suitable for potato chips. This study indicated that among the genotipes tested, there were superior clones were promissing for potato cultivation in indonesia. AbstrakAreal pertanaman kentang cenderung semakin meningkat namun hasil yang dicapai petani masih relatif rendah sehingga diperlukan upaya pemuliaan untuk memperbaiki varietas yang ada. Tujuan penelitian ialah mendapatkan informasi tentang daya hasil 41 klon kentang yang berpotensi hasil tinggi pada ekosistem dataran tinggi Ciwidey. Penelitian dilaksanakan di Kecamatan Ciwidey, Kabupaten Bandung elevasi 1.400 m di atas permukaan laut. Rancangan Percobaan yang digunakan ialah Rancangan Acak Kelompok dengan tiga ulangan. Jumlah klon yang diuji meliputi 41 klon harapan, termasuk varietas pembanding Granola. Populasi tanaman per plot terdiri atas 10 individu tanaman. Hasil pengamatan menunjukkan bahwa hasil kentang tertinggi diperoleh pada klon 29 (43,8 t/ha), 11 (38,2 t/ha), 31 (37,3 t/ha), 13 (36 t/ha), dan 35 (35,6 t/ha), sementara hasil varietas pembanding Granola hanya 23,4 t/ha. Klon 9, 22 dan 35 berpotensi sebagai bahan baku industri kripik kentang. Kegiatan ini menghasilkan klon-klon kentang unggul sebagai calon varietas baru yang memberikan kontribusi terhadap peningkatan produksi kentang di Indonesia.
Inbreeding pada Populasi Banteng (Bos javanicus d’Alton 1832) di Kebun Binatang Surabaya Reny Sawitri; Mariana Takandjandji
Buletin Plasma Nutfah Vol 18, No 2 (2012): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v18n2.2012.p84-94

Abstract

Inbreeding Population of Banteng (Bos javanicus d’Alton 1832) at Surabaya Zoo. Reny Sawitry and Mariana Takandjandji. Banteng (Bos javanicus d’Alton 1832) is one of wildlife that is maintained in Surabaya Zoo, their mating system happened from a couples, and so overlap from generation to next generation. The purposed of this research was to determine effective population size, genetic diversity, and the change of physical and physiological of the herd. The methods used in this study were analysis DNA mitochondria from hair samples, description of physical and physiological change, and inbreeding coefficient. The results showed that effective populations size of herd in Surabaya Zoo tended to decline from productive age of banteng. Haplotype diversity of herd population was very low, the distance of genetic intra population zerro, and it’s genetic diversity was very homogen. This occured caused change in sex ratio of which male dominated the offspring population. Subsequently, the impact of inbreeding was the change of physic and physiology of banteng such as skin colour, sterile and infertile. Inbreeding that happened in Surabaya Zoo affected extinction of third population because of individual number of live sex less than one. The inbreeding coeficient was calculate using of pedigree analysis and inbreeding rate per generation based on the population structure. The calculation result of inbreeding coeficient was 0.42, while the inbreeding rate was 4.3% per generation. Finally, it’s needed to supply banteng from nature to fix offsprings and it’s genetic diversity. AbstrakBanteng (Bos javanicus d’Alton 1832) merupakan salah satu satwa liar yang dipelihara di Kebun Binatang Surabaya (KBS). Sistem perkawinan banteng di lokasi ini dimulai dari bibit tunggal dan overlap antar generasi. Tujuan penelitian adalah untuk mengetahui keragaman genetika banteng di KBS melalui analisis DNA mitokondria, deskripsi perubahan fisik dan fisiologi banteng, gambaran nilai koefisien dan laju inbreeding per generasi. Metode yang digunakan adalah penghitungan ukuran populasi efektif, deskripsi perubahan fisik dan fisiologi serta koefisien dan laju inbreeding. Hasil penelitian menunjukkan bahwa ukuran populasi efektif banteng cenderung menurun dilihat dari umur produktif. Diversitas haplotipe populasi banteng di KBS sangat rendah, sehingga jarak genetik dalam populasi = 0 dan dapat dikatakan keragaman genetiknya sangat homogen. Rata-rata nilai koefisien inbreeding adalah 0,42 dan laju inbreeding 4,3% per generasi. Hal ini dapat dilihat dari keturunan jantan daripada betina. Perkawinan secara inbreeding penurunan fisik serta fisiologi banteng di KBS, seperti terjadinya kemandulan dan ketidaksuburan. Inbreeding juga mengakibatkan kepunahan pada populasi yang ada karena pada generasi ketiga jumlah individu tiap kelamin yang hidup kurang dari satu. Dengan demikian diperlukan pasokan banteng dari alam untuk memperbaiki keturunan dan keragaman genetik.
Pembentukan Genotipe Padi Berumur Sangat Genjah melalui Kultur Antera Iswari S. Dewi; A. Dinar Ambarwati; Aniversari Apriana; Atmitri Sisharmini; Ida H. Somantri; Bambang Suprihatno; Iman Ridwan
Buletin Plasma Nutfah Vol 18, No 2 (2012): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v18n2.2012.p54-61

Abstract

Development of Very Early Maturing Rice Genotypes through Anther Culture. Iswari S. Dewi, A. Dinar Ambarwati, Aniversari Apriana, Atmitri Sisharmini, Ida H. Somantri, Bambang Suprihatno, and Iman Ridwan. Rice is the most important food crop in Indonesia. Increase in production is needed due to population increase. Rice production in rainfed area is contributed the second after irrigated area. Rainfed condition requiring very early maturity (90-104 days) varieties. Rice anther culture can be applied to accelerate obtainment of doubled haploids (DHs) or pure lines needed in rice breeding. The experiment was aimed to obtain pure lines for developing very early maturing and high yielding rice varieties. Materials used for anther culture were F1s of Fatmawati/Kinamase, Inpari 1/Kinamase, Fatmawati/ Waseaikoku, Inpari 1/Waseaikoku, Fatmawati/IR71146, Inpari 1/IR71146, OM4495/Silugonggo, IR7146/Dodokan, and IR71730/OM1490. Anther culture media were N6 + NAA 2,0 mg/l + kinetin 0,5 mg/l for callus induction, MS+ NAA 0,5 mg/l + kinetin 2,0 mg/l for plantlet regeneration, and MS + 0,5 mg/l IBA for rooting. Putrescine 10-3 M was added to callus induction and regeneration media. The results shown that calli forming green plantlet (CFGP) were ranged from 0.25 to 83.33%. Fatmawati/Kinamase gave the highest CFGP (245 calli), followed by Inpari 1/Kinamase (78 calli) and Fatmawati/ Waseaikoku (68 calli). Total green plantlets obtained were 2.038 plantlets. After plantlet acclimatization and greenhouse grow-out, we obtained 507 DHs. The evaluation of 100 DHs at farmer field (Ciranjang District in Cianjur), based on their 50% heading date of 65 days, resulted in 33 lines cathegorized as very early maturing lines (+100 days). They were 18 lines from Fatmawati/Kinamase, 5 lines from Inpari 1/Kinamase, 8 lines from Fatmawati/Waseaikoku, and 2 lines from Inpari 1/ Waseaikoku. AbstrakPadi (Oryza sativa L.) merupakan komoditi pangan terpenting di Indonesia. Peningkatan produksi diperlukan seiring dengan peningkatan jumlah penduduk. Lahan sawah tadah hujan merupakan lumbung padi kedua setelah sawah irigasi. Kondisi lahan sawah tadah hujan memerlukan varietas-varietas padi berumur sangat genjah (90-104 hari). Teknik kultur antera dapat digunakan untuk mempercepat perolehan tanaman dihaploid (DH) atau galur murni dalam pemuliaan padi. Penelitian ini bertujuan untuk mendapatkan galur-galur murni yang akan digunakan dalam perakitan padi berdaya hasil tinggi dan berumur sangat genjah. Bahan tanaman yang digunakan untuk kultur antera adalah malai dari tanaman F1 hasil persilangan Fatmawati/Kinamase, Inpari 1/Kinamase, Fatmawati/Waseaikoku, Inpari 1/Waseaikoku, Fatmawati/IR71146, Inpari 1/ IR71146, OM4495/Silugonggo, IR7146/Dodokan, dan IR71730/OM1490. Media kultur antera adalah N6 + NAA 2,0 mg/l + kinetin 0,5 mg/l untuk media induksi kalus, MS+ NAA 0,5 mg/l + kinetin 2,0 mg/l untuk media regenerasi, dan MS + 0,5 mg/l IBA untuk media perakaran. Putresine 10-3 M ditambahkan pada media induksi kalus dan regenerasi. Hasil penelitian menunjukkan bahwa kalus yang menghasilkan tanaman hijau (KMTH) berkisar antara 0,25-83,33%. Persilangan Fatmawati/ Kinamase memberikan KMTH tertinggi (245 kalus), diikuti oleh Inpari 1/Kinamase (78 kalus) dan Fatmawati/ Waseaikoku (68 kalus). Total tanaman hijau yang diperoleh adalah 2.038 planlet dihaploid, namun diperoleh 507 tanaman setelah planlet diaklimatisasi dan tanaman ditumbuhkan di rumah kaca. Evaluasi terhadap 100 DH dilakukan di lahan petani Ciranjang, Cianjur. Berdasarkan hari berbunga 50% (65 hari setelah semai), diperoleh 33 galur yang termasuk kategori sangat genjah (dipanen +100 hari). Galur-galur tersebut adalah 18 galur dari persilangan Fatmawati/Kinamase, 5 galur dari persilangan Inpari 1/Kinamase, 8 galur dari persilangan Fatmawati/ Waseaikoku, dan 2 galur dari persilangan Inpari 1/ Waseaikoku.
Adaptasi dan Stabilitas Hasil Delapan Varietas Lokal Padi Sawah Abd. Aziz Syarif; Syahrul Zen
Buletin Plasma Nutfah Vol 18, No 2 (2012): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v18n2.2012.p62-69

Abstract

Adaptation and Yield Stability of Eight Local Lowland Varieties. Abd. Azis Syarif and Syahrul Zen. The availability of improved rice variety adapted to high altitude areas and suitable for consumers preference in West Sumatra is very limited. Majority of rice farmers in high altitude area in West Sumatra grow many adapted local varieties. This activity aimed at estimating yield adaptability and stability of selected local varieties as a requirement for variety release. The yield and other agronomic traits of eight local and one improved varieties (Batang Sumani) were evaluated at six locations in Solok District, using a Randomized Complete Block Design with three replications at each location. Regression technique of varietal yields at each location on environmental index (mean yield of all varieties at correspondent location) was used to assess the adaptability and stability of particular varieties. The results showed that local varieties of Caredek Putih and Caredek Merah gave the highest mean yields, 5.39 and 5.18 t/ha (13.87 and 9.28% higher than that of improved check variety) respectively. These varieties were found to be stable, indicated by non significantly different of their regression coefficient from 1.0 and deviation from regression from 0.0. AbstrakKetersediaan varietas unggul padi yang adaptif pada lahan sawah dataran tinggi dan memiliki rasa nasi yang sesuai dengan preferensi konsumen di Sumatera Barat masih sangat terbatas. Sampai saat ini, kebanyakan petani masih membudidayakan varietas-varietas lokal yang sudah beradaptasi dan disukai konsumen. Penelitian ini bertujuan untuk mengetahui potensi dan stabilitas hasil beberapa varietas lokal padi sawah untuk pengusulan pelepasan sebagai varietas unggul. Delapan varietas lokal padi sawah hasil identifikasi dan seleksi dari beberapa lokasi di Kabupaten Solok (Sumatera Barat) dan satu varietas unggul sebagai pembanding (varietas Batang Sumani) diuji daya hasil dan sifat agronomis lainnya di enam lokasi di Kabupaten Solok. Pada setiap lokasi digunakan rancangan percobaan Acak Kelompok dengan tiga ulangan. Pendugaan adaptabilitas dan stabilitas hasil menggunakan teknik regresi dimana rataan hasil setiap varietas di setiap lokasi diregresikan dengan indeks lingkungan (rataan hasil semua varietas) lokasi yang bersangkutan. Hasil penelitian menunjukkan bahwa varietas local Caredek Putih dan Caredek Merah memberi rataan hasil tertinggi, yakni 5,93 dan 5,18 t/ha (13,8 dan 9,28% lebih tinggi dari varietas pembanding). Kedua varietas ini adaptabilitas umum atau beradaptasi baik pada semua lingkungan dan stabil, ditunjukkan oleh koefisien regresinya yang tidak berbeda nyata dari 1.0 dan simpangan dari regresi yang tidak bebeda nyata dari 0.
Keunggulan dan Potensi Pengembangan Sumber Daya Genetik Durian Kalimantan Tengah Amik Krismawati
Buletin Plasma Nutfah Vol 18, No 2 (2012): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v18n2.2012.p70-76

Abstract

Superiority and Potential Development of Durio Germplasm in Central Kalimantan. Amik Krismawati. Indonesia is rich of genetic resources and biodiversity of Durio spp., but its development no so fastly as in Thailand. This is due to plant durio has not managed commercially and it grew hundred years and it origin from generative propagation, caused the durio plantation variety heterogen. To determine the performance and advantage of the diversity of genetic resources should be conducted observations of durio related species the characterization in each district centre. Observation and characterization of related species of durio e.i. Durio zibethinus M. (durian), Durio kutejensis H.B (papaken), Durio oxleyanus (kerantungan/taetungen), and Durio dulcis (layung) were conducted in four districts of Central Kalimantan from August 2004 to January 2005. Seasons fruity and yield of each species to variously among the durio and its related species. Differences among accesions were characterized by fruit type, fruit shape, fruit skin texture, fruit length, fruit skin colour, fruit flesh colour, and taste. The fruiting season and fruit production of each species also varied durio relatives.The result of characterization showed the related species of Durio are indigenous of Indonesia and potencially to development in the future. AbstrakIndonesia kaya akan sumber plasma nutfah dan keragaman jenis durian (Durio spp.), namun pengembangannya tidak sepesat Thailand. Hal ini disebabkan tanaman durian belum dikelola secara komersial dan tanaman yang ada umumnya sudah berumur ratusan tahun yang berasal dari perbanyakan generatif sehingga tidak memberikan hasil yang optimal. Untuk mengetahui keunggulan dari keanekaragaman sumber daya genetik durian maka dilakukan observasi dan karakterisasi di beberapa lokasi di empat kabupaten di Kalimantan Tengah dari Agustus 2004 hingga Januari 2005. Kerabat durian yang diobservasi dan dikarakterisasi adalah durian (Durio zibethinus M.), papaken (Durio kutejensis H.B.), kerantungan/taetungen (Durio oxleyanus), dan layung (Durio dulcis). Hasil karakterisasi menunjukkan bahwa karakter buah antarspesies kerabat durian sangat bervariasi. Perbedaan di antara spesies tersebut terletak pada tipe buah, bentuk buah, tekstur kulit buah, panjang buah, warna kulit buah, warna dan rasa daging buah. Musim berbuah dan produksi masing-masing spesies kerabat durian juga bervariasi. Kerabat durian dari jenis Durio (Durio spp.) yang merupakan tanaman asli Indonesia berpotensi dikembangkan.

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