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WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences
Published by Indonesian Center for Animal Research and Development
ISSN : 02166461     EISSN : 23546832     DOI : 10.14334
Core Subject : Health,
Veterinary
WARTAZOA. Indonesian Bulletin of Animal and Veterinary Sciences ISSN: 0216-6461 E-ISSN: 2354-6832 is a peer-reviewed, scientific journal published by Indonesian Center for Animal Research and Development (ICARD). The aim of this journal is to publish high-quality articles dedicated to all aspects of the latest outstanding developments in the field of animal and veterinary science. It was first published in 1983. The journal has been registered in the CrossRef system with Digital Object Identifier (DOI) prefix 10.14334.
Arjuna Subject : -
Articles 5 Documents
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Search results for , issue " Vol 21, No 1 (2011)" : 5 Documents clear
Molecular Phylogenetic: Organism Taxonomy Method Based on Evolution History Dharmayanti, N.L.P Indi
Indonesian Bulletin of Animal and Veterinary Sciences Vol 21, No 1 (2011)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (283.91 KB) | DOI: 10.14334/wartazoa.v21i1.948

Abstract

Phylogenetic is described as taxonomy classification of an  organism based on its evolution history namely its phylogeny and as a part of systematic science that has objective to determine phylogeny of organism according to its characteristic. Phylogenetic analysis from amino acid and protein usually became important area in sequence analysis. Phylogenetic analysis can be used to follow the rapid change of a species such as virus. The phylogenetic evolution tree is a two dimensional of a species graphic that shows relationship among organisms or particularly among their gene sequences. The sequence separation are referred as taxa (singular taxon) that is defined as phylogenetically distinct units on the tree. The tree consists of outer branches or leaves that represents taxa and nodes and branch represent correlation among taxa. When the nucleotide sequence from two different organism are similar, they were inferred to be descended from common ancestor. There were three methods which were used in phylogenetic, namely (1) Maximum parsimony, (2) Distance, and (3) Maximum likehoood. Those methods generally are applied to construct the evolutionary tree or the best tree for determine sequence variation in group. Every method is usually used for different analysis and data. Key words: Phylogenetic, analysis, evolution, nucleotide/protein sequence
Use of Polymerase Chain Reaction Enzyme Linked Oligonucleotide Sorbent Assay (Pcr-Elosa) for Detection of Disease Agents Tarigan, Simson
Indonesian Bulletin of Animal and Veterinary Sciences Vol 21, No 1 (2011)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (252.898 KB) | DOI: 10.14334/wartazoa.v21i1.949

Abstract

Diagnostic tool comprises one of the vital components in the control of  infectious diseases. One of the most common techniques in the diagnosis of infectious disease currently available is the polymerase chain reaction (PCR) because this technique is very sensitive, specific, and rapid. This technique requires an adjunct technique to indicate the formation of the right reaction product. Agarose gel electrophoresis has been the most common technique to visualise the PCR product or amplicon. Enzyme linked oligonucleotide sorbent assay (ELOSA) is an alternative technique which is more sensitive and gives more important identity of the amplicon. This technique can be more than 100 times as sensitive as a gel agarose  electrophoresis, and very specific since confirmation of the amplicon is carried out by DNA hibridisation. The capacity of the ELOSA can also be extended to the detection of disease-causal agent at subtype level, or detection of mutation at particular location in a gene. Since the equipment used for ELOSA is similar to that for ELISA (enzyme linked immunosorbent assay), a large number of samples can be accomplished rapidly. As in ELISA, a number of variation can be made in ELOSA depend on the requirement. Nucleotide can be immobilised on the microwell plate either by passive adsorbtion, by first conjugation of  nucleotide with biotin then immobilisation on streptavidin-coated microwell plate, or immobilisaion by covalent bonding. The PCR and ELOSA can be performed at separate or in a single tube by first immobilising the PCR primers on the surface of microwell plates. Key words: PCR amplicon, agarose electrophoresis, oligonucleotide immobilisation, DNA hybridisation
Bovine Viral Diarrhea in Cattle in Indonesia and its Problems ., Sudarisman
Indonesian Bulletin of Animal and Veterinary Sciences Vol 21, No 1 (2011)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (178.455 KB) | DOI: 10.14334/wartazoa.v21i1.950

Abstract

Bovine Viral Diarrhea (BVD) is a disease caused by the bovine viral diarrhea virus (BVDV), an ubiquitous, easily transmitted virus with worldwide distribution. The majority of postnatal infections with BVDV are nonclinical, with biphasic temperature elevation and leucopenia followed by a spesific immune response measurable by serum neutralisation test. The infection can be diagnosed serologically or virologically and the disease is recognized by clinical signs and pathological lesions. Disease control is based on the use of modified live virus (MLV) vaccines. Opinions vary on the relative economic importance of BVD, depends on the appropriateness and timing of vaccination in various production-management system. Key words: Bovine viral diarrhea, cattle, disease control, vaccine
Anticipating the Emerging of Some Strategical Infectious Animal Diseases in Indonesia Related to The Effect of Global Warming and Climate Change Bahri, Sjamsul; Syafriati, T
Indonesian Bulletin of Animal and Veterinary Sciences Vol 21, No 1 (2011)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (274.107 KB) | DOI: 10.14334/wartazoa.v21i1.951

Abstract

The effect of global warming and climate change is changing the season, included flooding in one area and very dry in other area, changing the temperature and humidity. These changes will trigger changing of the life of biological agent (virus, bacteria, parasites and so on), variety of animal species, variety of vectors as reservoir host of animal with the role of transmitting the disease to other animal species, This condition will trigger the new animal disease (emerging disease) or old disease will be re-emerged (re-emerging diseases). This paper will discuss the effect of global warming and climate change on animal diseases in Indonesia such as Bluetongue (BT), Nipah, Japanese encephalitis (JE), West Nile (WN), and Rift Valley fever (RVF). The climate changes such as increasing the earth temperature and rainfall will cause extremely increase of vector population for BT, JE, WN and RVF. In addition, animal transportation and bird migration from one country to others or region will cause changing of ecological system and will open the chance to distribute the diseases. Hence, anticipation on those disease outbreaks should be taken by conducting the surveilance and early detection to those diseases. The possibility of entering Nipah disease in Indonesia should be anticipated because the avaibility of Nipah virus and the reservoir host (Pteropus spp) and also pigs as amplifier host in the surrounding area. Other diseases such as, leptospirosis, anthrax and avian influenza (H5N1) are also have a wider potential to distributing the disease related to the climate change in Indonesia. Key words: Global warming, climate change, zoonotic disease
Development of Recombinant Vaccine Using Herpesvirus of Turkey (Hvt) as Vector for Several Viral Diseases in Poultry Industry Hartawan, Risza
Indonesian Bulletin of Animal and Veterinary Sciences Vol 21, No 1 (2011)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (464.365 KB) | DOI: 10.14334/wartazoa.v21i1.952

Abstract

Herpesvirus of turkey (HVT) has been utilised as live vaccine against Marek’s disease in poultry industry world-wide for many years. However, the potency of HVT is not limited on the Marek’s disease only. Along with rapid development of recombinant technique, the potency of HVT can be broaden for other diseases. As naturally apathogenic virus, HVT is a suitable candidate as vector vaccine to express important antigens of viral pathogens. Several researches have been dedicated to design HVT recombinant vaccine by  inserting gene of important virus, such as Marek’s disease virus (MDV), immuno bursal disease virus (IBDV), Newcastle disease virus (NDV) and Avian Influenza virus (AIV). Therefore, the future recombinant of HVT has been expected to be better in performance along with the improvement of recombinant technique. Key words: Herpesvirus of turkey, live vector vaccine, viral pathogens

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