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INDONESIA
Jurnal Ternak Tropika
Published by Universitas Brawijaya
ISSN : 25031007     EISSN : 25031007     DOI : -
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TERNAK TROPIKA, Journal of Tropical Animal Production (JTAP) provides for rapid publication of full-length papers, short communication and review articles describing of new finding or theory in Animal Production area. TERNAK TROPIKA has 1 volume with 2 issues per year. TERNAK TROPIKA is published by Department of Animal Priduction, Faculty of Animal Husbandry, Brawijaya University Indonesia in collaboration with Indonesian Society of Animal Science (ISPI
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Search results for , issue "Vol 13, No 1 (2012): Ternak Tropika" : 8 Documents clear
PENGARUH PENAMBAHAN FILLER KOMPOSIT (WHEAT BRAN DAN POLLARD) DAN RUMPUT LAUT TERHADAP pH, WHC, COOKING LOSS DAN TEKSTUR NUGGET KELINCI Ariadi Tricahyo; Aris Sri Widati; Eny Sri Widyastuti
TERNAK TROPIKA Journal of Tropical Animal Production Vol 13, No 1 (2012): Ternak Tropika
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

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Penelitian ini bertujuan untuk mengetahui persentase terbaik penambahanfiller komposit (wheat bran dan pollard) serta rumput laut dalam pembuatan nuggetkelinci ditinjau dari pH, WHC, cooking loss, dan tekstur. Materi yang digunakandalam penelitian ini adalah nugget yang dibuat dari daging kelinci dengan penambahanfiller komposit (wheat bran dan pollard) serta rumput laut, tapioka, telur, garam,bawang putih, merica, dan air. Metode yang digunakan adalah percobaan denganmenggunakan Rancangan Acak Kelompok (RAK) pola faktorial. Faktor pertamaadalah penambahan filler komposit (wheat brand dan pollard dengan perbandingan1:1) sebanyak 4 tingkat yaitu: 0% (F0), 10% (F1), 20% (F2), dan 30% (F3). Faktorkedua adalah penggunaan rumput laut sebanyak 3 tingkat yaitu: 0% (T0), 20% (T2)dan 40% (T4). Variabel yang diukur pada penelitian ini adalah pH, WHC, cookingloss, dan tekstur. Hasil penelitian menunjukkan bahwa penambahan filler kompositpada nugget kelinci tidak berpengaruh nyata (P>0,05) terhadap pH, WHC, cookingloss, dan tekstur, sedangkan penambahan rumput laut pada nugget kelinci,berpengaruh sangat nyata (P<0,01) terhadap pH, WHC, cooking loss, dan tekstur.Kombinasi penambahan filler komposit dan rumput laut pada nugget kelinci tidakberpengaruh nyata (P>0,05) terhadap pH, WHC, cooking loss, dan tekstur. Rata-ratapH pada nugget kelinci dengan penambahan filler komposit (wheat bran danpollard) dan rumput laut antara 6,158 sampai 6,292. Rata-rata persentase WHC antara42,19% sampai 47,01%, rata-rata persentase cooking loss antara 2,76 % sampai 2,81%, Rata-rata tekstur antara 0,52N sampai 1,08N. Disimpulkan bahwa perlakuanterbaik dihasilkan pada nugget kelinci dengan penambahan rumput laut 40% dan tanpapenambahan filler komposit dengan nilai pH sebesar 6,3 ; nilai WHC 46,68% ;Cooking Loss 2,79%, tekstur 0,74 N. Disarankan untuk memproduksi nuggetkelinci dengan penambahan rumput laut 40% dan tanpa filler komposit agarmemberikan hasil yang optimal.Kata kunci: Nugget kelinci, filler komposit, rumput lautTHE EFFECT OF ADDITION OF COMPOSITE FILLER (WHEAT BRANAND POLLARD) AND SEAWEED ON pH, WHC, COOKING LOSS ANDTEXTURE OF RABBIT NUGGETSABSTRACTThe objective of this research was to find out the best percentage of fillercomposite and seaweed adding in the rabbit nuggets on the level pH, WHC, cookingloss, and texture. The materials of the research consist of nuggets made of rabbit meatwhich was added by filler composite (wheat bran and pollard) and seaweed, tapioca,egg, salt, garlic, pepper, and water. Meanwhile, this research used experimentalmethod with random grouping design (RAK), there were 2 factors. The first factor wasthe addition of filler composite (wheat bran and pollard in the ratio 1:1) by 4 levels are:0% (F0), 10% (F1), 20% (F2), and 30% (F3). The second factor use of seaweed by 3levels are: 0% (T0), 20% (T2) and 40% (T4). The parameter measured were pH,WHC, cooking loss, and texture. The result of research was that the addition of fillercomposite has no significantly effected rabbit nuggets (P>0.05) on pH, WHC, cookingloss, and texture. while the addition of seaweed rabbit nuggets has highly significanteffect (P<0.01) on pH, WHC cooking loss, and texture. Combination of the addition offiller composite and seaweed in rabbit nuggets has no significant effect (P>0.05) onpH, WHC, cooking loss, and texture. It’s suggested to produce rabbit nuggets withoutfiller composite and seaweed 40%, to give more optimal result.Keyword: Rabbit nuggets, filler composite, seaweed
ANALISIS STRUKTUR BIAYA DAN PERBEDAAN PENDAPATAN USAHA TERNAK AYAM RAS PEDAGING PADA POLA DAN SKALA USAHA TERNAK YANG BERBEDA DI KOTA KENDARI PROVINSI SULAWESI TENGGARA D.I Bahari; Z, Fanani; B.A. Nugroho
TERNAK TROPIKA Journal of Tropical Animal Production Vol 13, No 1 (2012): Ternak Tropika
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

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Penelitian ini bertujuan untuk menganalisis karakteristik struktur biaya usaha ternakayam ras pedaging dan perbedaan tingkat pendapatan peternak antara pola usaha danskala yang berbeda. Penelitian dilakukan di Kota Kendari sulawesi tenggara padaSeptember-November 2011. Enam puluh tiga responden yang ditentukan denganmetode sensus pada populasi peternak. Data penelitian diestimasi dengan (1) analisisdeskriptif dengan tambahan data kuantitatif sederhana seperti tabel distribusi frekuensi,(2) tingkat pendapatan peternak dengan metode R/C ratio, (3) Uji-t Berbeda Mean.Hasil analisis menunjukkan besar kecilnya skala menentukan tingkat biaya usahaternak. Skala yang lebih besar akan membutuhkan tingkat biaya produksi yang lebihbesar yang besar pula, namun dapat menurunkan biaya per satuan output produksi.Secara keseluruhan tingkat R/C ratio peternak lebih dari 1 dengan rata-rata 1,14 namuntingkat R/C ratio pada pola usaha mandiri lebih tinggi dari pola usaha kemitraan.Tingkat pendapatan peternak pola usaha kemitraan sama dengan tingkat pendapatanpeternak pola usaha mandiri pada usaha ternak ayam ras pedaging di Kota Kendari,namun baik pada pola usaha kemitraan dan pola usaha mandiri tingkat pendapatanpeternak skala besar lebih tinggi daripada tingkat pendapatan peternak skala kecil padausaha ternak ayam ras pedaging di Kota Kendari.Kata kunci : Karakteristik Struktur Biaya, Tingkat Pendapatan, Pola UsahaKemitraan dan MandiriCOST OF BROILER FARMS CHARATERISTIC AND INCOMEDIFFERENCES ANALYSIS OF BROILER FARM DIFFERENT OF PATTERNAND SCALE FARM IN KOTA KENDARI SOUTH-EAST SULAWESIPROVINCEABSTRACTThis research was aimed to analyze the cost of broiler farms charateristic anddifference of income rate between pattern of farm and farm scale. Research werelocated in Kota Kendari Southeast Sulawesi while September to November 2011. Sixtythree respondents were determined with census method in farmer population. Researchdata was estimated by : (1) discriptive analysis with additional quantitative data assimple as a frequency distribution table, (2) income rate of farmers with the R / CRatio, (3) independet sample t-test. The result showed that size of scale determine costof broiler farms amount. Large scale would require more amount of production cost,but it could reduce cost per unit of production output. Overall, The overall rate of R/Cratio was more than 1 with an average of 1.14 but the independent farming rate of R/Cratio was higher than partnerships farming. Income rate of partnership farming wasequal with income rate of independent farming, but both of partnerships farming andindependent farming, the income rate of large-scale farmer was higher than incomerate of small-scale farmer in broiler farm Kota Kendari.Keywords : Cost of Broiler Farms Charateristic, Income Rate, Partnership AndIndependent Pattern of Farm.
SELEKSI PEJANTAN BERDASARKAN NILAI PEMULIAAN PADA SAPI PERANAKAN ONGOLE (PO) DI LOKA PENELITIAN SAPI POTONG GRATI – PASURUAN P.W. Prihandini; L. Hakim; V. M. A Nurgiartiningsih
TERNAK TROPIKA Journal of Tropical Animal Production Vol 13, No 1 (2012): Ternak Tropika
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

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Abstract

Penelitian tentang seleksi pejantan sapi PO berdasarkan nilai pemuliaannyatelah dilaksanakan di kandang percobaan Loka Penelitian Sapi Potong Grati Pasuruan,pada bulan Juni sampai November 2011. Tujuan penelitian untuk memperolehinformasi peringkat keunggulan pejantan sapi PO berdasarkan nilai pemuliaan bobotbadan terkoreksi umur 205 dan 365 hari. Materi yang digunakan adalah data rekordingproduksi sapi PO umur 205 dan 365 hari dari 310 ekor, keturunan dari 27 ekorpejantan dan 163 ekor induk. Metode yang digunakan adalah studi kasus denganmenganalisis data rekording sapi PO tersebut di lokasi penelitian (tahun 2004-2010).Variabel yang diamati adalah bobot badan umur 205 dan 365 hari; yang selanjutnyadilakukan koreksi untuk keperluan estimasi nilai pemuliaannya berdasarkan saudaratiri (half-sib). Hasil penelitian menunjukkan bahwa rataan bobot badan sapi PO umur205 hari sebesar 109,10+18,35 kg dan umur 365 hari sebesar 132,70+19,93 kg.Pejantan nomor 5 mempunyai nilai pemulian bobot badan umur 205 hari yangtertinggi; sedangkan nilai pemuliaan bobot badan umur 365 hari dimiliki pejantannomor 12. Dari hasil uji peringkat terdapat korelasi yang nyata (P<0,01) antaraperingkat pejantan berdasarkan nilai pemuliaan bobot badan umur 205 dan 365 hari.Disimpulkan bahwa pejantan nomor 5 menduduki peringkat pertama dari semuapejantan yang diuji, dengan nilai pemuliaan bobot badan umur 205 dan 365 harimasing-masing pada peringkat pertama dan ketiga.Kata kunci: nilai pemuliaan, pejantan, sapi Peranakan Ongole (PO)SIRE SELECTION BASED ON BREEDING VALUE IN PO CATTLE ATCATTLE RESEARCH CENTRE GRATI - PASURUANABSTRAKResearch on sire selection based on breeding value in PO cattle was conductedfrom June to November 2011 in Cattle Research Station, Grati, Pasuruan. Theobjective of this research was to find out the ranking of sire superiority based onbreeding value of 205 and 365 days corrected body weight. Materials used were 310production records of PO cattle at 205 and 365 days of age, which resulted from 27sires and 163 dams. Method used was case study with analyzing the data collectedfrom 2004-2010. Variables measured were body weight at 205 and 365 days of age,which then were corrected for estimating breeding value based on half-sib relationship.Results showed that mean of body weight of PO cattle at 205 and 365 days of age were109.10+18.35 kg and 132.70+19.93 kg, respectively. The best sire for body weight at205 days of age was sire number 5 and that at 365 days of age was sire number 12.There was significant rank correlation (P<0.01) between breeding value of bodyweight at 205 and that at 365 days of age. The conclusion was that sire number 5 wasthe best sire, with breeding value of body weight at 205 and 365 days of age at the firstand third rank, respectively.Key words: breeding value, sire, PO cattle
PENGARUH UMUR INDUK TERHADAP POTENSI KETERSEDIAAN SUMBER OOSIT KAMBING Moh Nur Ihsan
TERNAK TROPIKA Journal of Tropical Animal Production Vol 13, No 1 (2012): Ternak Tropika
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

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Suatu penelitian dengan tujuan untuk mengetahui mengetahui potensiketersediaan oosit berdasarkan kelompok umur kambing telah dilakukan, dandiharapkan akan bermanfaat sebagai informasi bagi perkembangan teknologireproduksi, khususnya penyediaan oosit matang di laboratotium.Penelitian dilakukan dengan metode percobaan laboratorium, menggunakansampel ovarium kambing dari Rumah Potong kambing, yang dikelompokkan masingmasingkambing dengan umur <1 th,1-14 th dan > 4 th.Variabel yang diamati ukurandan jumlah folikel antral.Disimpulkan bahwa kelompok umur induk berpengaruh nyata terhadap potensipenyediaan oosit, dimana induk dengan umur > 4 th menghasilkan oosit tertinggi.Kata Kunci: umur induk kambing dan potensi ketersediaan oositINFLUENCES OF AGES GOAT ON POTENTIAL OVARIES FORPROVIDING OOCYTES GOATSABSTRACTThe research with aim to study the potential of different age female goats onproviding oocytes was carry out. It was expected give information about reproductivetechnology development especially providing laboratory oocytes.Research was conducted by experiment laboratory method, with abatoir ovaryfemale goats sample with grouped ages goat < 1 year, 1-4 year and > 4 year. Variablewas observed ovary size and number follicle of ovaries.It was concluded that ages of goat significant difference to potentials providingoocyte where age 4 year goat highest produced oocytes.Keywords: ages of goat and potentials providing oocytes.
VARIASI FENOTIPE F1 CROSSBREED DARI HASIL PERSILANGAN BURUNG BLACK THROAT DENGAN BERBAGAI BURUNG KENARI LOKAL (Serinus Canaria) Mudawamah Mudawamah; Susilowati S; Trijaya Trijaya
TERNAK TROPIKA Journal of Tropical Animal Production Vol 13, No 1 (2012): Ternak Tropika
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

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Burung kenari merupakan burung berkicau yang dipelihara bukan hanya untukhobi tetapi sudah mengarah ke komersial dengan harga jualnya ditentukan oleh warnabulu, ukuran tubuh dan suara. Tujuan penelitian ini adalah untuk mengetahui variasifenotipe F1 crossbreed hasil persilangan kenari lokal dengan Black throat. Metodepenelitian adalah eksperimen dan data dianalisis menggunakan Chi-square. Materipenelitian yang digunakan satu ekor pejantan burung black throat, sembilan ekorindukan burung kenari lokal warna bulu hijau, starblue dan kuning. Perkawinandilakukan dengan perkawinan alam dengan ulangan tiga kali. Variasi fenotipe yangdiamati adalah warna bulu, ukuran tubuh dan suara. Hasil penelitian menunjukkanbahwa F1 crossbreed mempunyai postur tubuh lebih besar dari Black throat dan lebihkecil dari kenari lokal dengan suara lebih tinggi dan tebal dibandingkan dengan kenarilokal serta warna bulunya merupakan kombinasi kenari lokal dengan Black throat.Dari hasil análisis dengan chi-square menunjukkan bahwa variasi fenotipe persilanganblack throat dengan berbagai kenari lokal tidak berbeda nyata (P > 0.05) denganstándar penilaian Malang Canary Club. Tetapi ada kecenderungan bahwa skor hasilpersilangan antara black throat dengan kenari kuning (26,6) menghasilkan rata-rataskor terendah dibandingkan dengan hijau (27) dan starblue (27,55).Kata Kunci : kenari, persilangan, variasi fenotipe.(PHENOTYPE VARIATION OF F1 CROSSBREED DERIVED FROM BLACKTHROAT WITH LOCAL CANARY BIRDS (Serinus Canaria))ABSTRACTBirds singing canary is maintained not just for a hobby but has already led tothe commercial and selling price determined by feather color, body size and voice. Thepurpose of this study was to determine the phenotype variation of F1 crossbreed whichwas the result of crossbreeding of local canary with Black throat. The research methodwas experimental and data were analyzed using Chi-square test. Research materialsused one tail of black throat male, nine tails of local canary female of feather color ofgreen, and yellow starblue. Mating performed by natural mating with threereplications. Phenotypic variation observed was the color of hair, body size and voice.The results showed that F1 crossbreed had a bigger body posture of Black throat andsmaller than the local canary with a voice higher and thicker than the local canary andthe feather color was a combination of local with Black throat. From the results of thechi-square analysis showed that phenotypic variation F1 crossbreed were notsignificantly different (P> 0.05) with a standard assessment of Malang Canary Club.But there is a tendency that the score from crosses between the black throat with ayellow canary (26.6) yielded the lowest average score compared with the green canary(27) and starblue canary (27.55).Key words: canary, crosses, phenotypic variation.
PENGARUH LAMA SIMPAN SEMEN TERHADAP KUALITAS SPERMATOZOA AYAM KAMPUNG DALAM PENGENCER RINGER’S PADA SUHU 4 C D.R. Danang; N. Isnaini; P. Trisunuwati
TERNAK TROPIKA Journal of Tropical Animal Production Vol 13, No 1 (2012): Ternak Tropika
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

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Tujuan penelitian adalah untuk mengetahui pengaruh lama simpan semen padasuhu 40C terhadap kualitas spermatozoa ayam kampung dalam pengencer Ringersolution. Hasil penelitian diharapkan berguna sebagai informasi tentang waktu optimallama simpan spermatozoa ayam kampung dalam pengencer Ringer solution pada suhu40C sehingga dapat menunjang keberhasilan IB. Materi yang digunakan adalah semenayam Kampung berasal dari empat ekor ayam kampung jantan berumur 1 - 1,5 tahun,bobot badan ± 2,2 kg. Penelitian dilakukan menggunakan metode percobaan denganperlakuan larutan pengencer Ringer’s pada suhu 4 0C menggunakan enam waktupengamatan yang berbeda yaitu pada jam ke 0, 6, 12, 18, 24 dan 30. Ulangan 10kali. Data yang diperoleh dianalisis dengan analisis ragam. Rancangan yangdigunakan adalah Rancangan Acak Lengkap (RAL), apabila hasil yang diperolehmenunjukkan perbedaan pengaruh yang nyata (P<0,05) atau sangat nyata (P<0,01)maka dilanjutkan dengan Uji Beda Nyata Terkecil (BNT). Parameter yang diamati:motilitas individu, viabilitas dan abnormalitas spermatozoa. Hasil Penelitianmenunjukkan bahwa lama simpan pada suhu 40C memberikan pengaruh yang sangatnyata (P<0.01) terhadap kualitas spermatozoa ayam kampung yang meliputi motilitasindividu, persentase viabilitas, dan persentase abnormalitas dalam pengencer Ringer.Kualitas semen dapat dipertahankan sampai 18 jam yang memiliki kualitas semen yaitu: motilitas individu 47 + 5.87, viabilitas 69.4 + 3.34 dan abnormalitas 15 + 0.82.Disimpulkan bahwa kualitas semen ayam Kampung yang diencerkan dengan Ringer’syang disimpan pada suhu 4 0C mengalami penurunan secara bertahap dan dapatdigunakan untuk IB dalam waktu tidak lebih dari 18 jam setelah penampungan.Kata kunci : kualitas spermatozoa ayam Kampung , pengencer Ringer’s , suhu 4 0CTHE EFFECT OF STORAGE TIME ON NATIVE CHIKENSSPERMATOZOA QUALITY BY RINGER SOLUTION DILUENT IN 4 0CTEMPERATUREABSTRACTThe aim of the study was to find out endurance of Kampung chicken semen qualitydiluted by Ringer’s of various storage time at 4 0C. The result of the study can be usedas infomation resource about the optimal time to store the Native chicken spermatozoawith Ringer’s diluent at 4 0C to support the successful of Artificial Inseminationprogram. The research material was semen of Native chicken from four rooster from 1-1.5 years old and about ± 2.2 kg body weight. The research was design byCompletely Randomized Design (CRD) with 6 treatment and 10 replication, if therewere significant influence, it would be tested by least significant Different Test. Thevariable’s observed were individual motility, sperm viability, and sperm abnormality.The result showed that the quality of semen in Ringer’s diluter significantly influencedby long storage at 4 0C temperature. Semen quality can be used up to 18 hours with:47 ± 5.87 individual motility, 69.4 ± 3.34 percentage of viability, and 15 ± 0.82abnormalities. The conclution is the quality of native chicken semen decreasegradually in the ringer solution at 4oC storage.To support the successful of AI, it willbe better if the semen of Native chicken diluted by Ringer’s and stored not more than18 hours at 4 0C temperature.
PERTAMBAHAN BOBOT BADAN HARIAN SAPI BRAHMAN CROSS PADA BOBOT BADAN DAN FRAME SIZE YANG BERBEDA Ardhina Firdausi; T. Susilawati; M. Nasich; Kuswati Kuswati
TERNAK TROPIKA Journal of Tropical Animal Production Vol 13, No 1 (2012): Ternak Tropika
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

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This research was conducted at PT. Widodo Makmur Perkasa Feedlot onAugust until October 2011. The purpose is to assess average daily gain of Brahmancross with frame sizetype(M andL) anddifferentbodyweights(<300 kg, 300-350kg,and>350 kg) and theinteractionbetween theframe sizeanda differentbodyweights.Thematerialsused for this research were 3052 head SteerBrahmanCrosscattle with initialbody weights250 kg-380 kg. This research was using study casus. data obtained wassecondary data. The obtained data was analyzed using Completely Random Design(CRD) inbalanced factorial.The result showed that frame size has significant effect(P<0,01) on Brahman cross steer average daily gain. The average daily gain is 1.15 ±0.367 kg for M frame size group and 1.3 ± 0.354 kg for L frame size group. Initialbody weight was also has a significant effect on Brahman cross steer average dailygain. The average daily gain of <301; 301-350; and >350 group were 1.329±0.366;1.22±0.348; and 1.13±0.357 kg. The interaction between frame size and initial bodyweight give significant effect on Brahman cross steer daily gain. The gain of <301kg ofinitial body weight group with L framesize is 1.34 kg/day and M frame size is 1.29kg/day. The gain of 301-350kg of initial body weight group with L frame is 1.31kg/day. And the gain of >350kg of initial body weight group with L frame size is 1.21kg/day and M frame size is 0.93 kg/day.Key word : Average Daily Gain, Brahman cross, Frame size, Initial Body Weight
STUDI SITOGENETIK TERNAK LOKAL UNTUK STANDARISASI KROMOSOM DAN DETEKSI ABNORMALITAS GENETIK TERNAK RUMINANSIA LOKAL Gatot Ciptadi; M, Nur Ihsan; V.M. Ani Nurgiartiningsih
TERNAK TROPIKA Journal of Tropical Animal Production Vol 13, No 1 (2012): Ternak Tropika
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

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Abstract

Analisis kromosom ternak lokal di Indonesia sangat penting artinya karenamasih sangat terbatasnya data-data genetik dasar yang ada selama ini. Bagi ternak bibitanalisis kromosom perlu dilakukan untuk mendeteksi kemungkinan munculnya cacatgenetik yang heriditer. Hal ini perlu dicermati mengingat bahwa peluang pewarisankepada generasi berikutnya adalah sangat besar terkait jumlah anak keturunan yangbisa dihasilkan dari seekor pejantan. Tujuan dari penelitian ini adalah untukmenganalisis kromosom ternak ruminansia lokal di Indonesia yaitu sapi, kerbau dankambing. Pada ternak lokal Indonesia masih sangat terbatas dilakukan analisiskromosom, padahal sangat penting terutama bagi ternak bibit. Hasil penelitian ini dapatdigunakan sebagai bahan pertimbangan bagi strategi peningkatan kualitas genetikternak ruminansia lokal.Metode digunakan standart kariotyping menggunakan sampel darah (wholeblood/) dengan G banding. Kariotyping dilakukan dengan dasar standart yang sudahada. Preparasi kromosom menggunakan medium Karyo MAX (GIBCO/BRL),Colcemic Solution, Giemsa Stain dan Potasium chloride solution. Kultur sel dilakukanberdasarkan medode standar karyotyping mamalia. Minimal jumlah 5 buah spreadingMetafase II kromosom terbaik, dilakukan microfotografi dan kemudian dilakukananalisis kromosom dengan software cytovision image analysis, ditentukan normaltidaknya kromosom berdasarkan standart kariotyping.Hasil Penelitian ini tidak ditemukan ternak ruminansia dengan abnormalitasjumlah kromosom, sehingga bisa diartikan bahwa tidak ada beberapa abnormalitaskromosom karena genetik seperti translokasi roberston (2N=58) atau kelainan jumlahkromosom yang lain. Pada semua ternak yang diamati kromosomnya ditemukankromosom 2 N (sapi Madura 2 N = 60), kerbau (swamp buffalo, 2 N=50 ) danKambing (kambing PE dan kacang 2 N= 60) yang terdiri atas 58, 48 dan 58 autosomdan 2 seks kromosom. Analisis perlu ditingkatkan ketelitiannya menggunakan teknikFISH, immunofluorescent, cytovision image analysis dilengkapi soft ware yang sesuai.Ruminansia lokal Indonesia perlu dilakukan penyusunan standart kariotyping,khususnya pada ruminansia yang diproduksi sperma bekunya untuk keperluanimplementasi Inseminasi Buatan, sangat direkomendasikan untuk dilakukankariotyping sebagai jaminan normalitas genetik serta bebas cacat genetik heriditer.Kata Kunci: Kariotyping, Kromosom, Ruminansia, Abnormalitas Genetik.CYTOGENETIC ANALYSIS FOR KARYOTYPE STANDARITATION ANDDETECTION OF GENETIC ABNORMALITY OF LOCAL RUMINANTABSTRACTOn the basis of the important of chromosome abnormalites and their negative effectin the near future, chromosomal investigation of breeding domestic animals and theirprogeny began in different countries. Chromosomal abnormality are usually consideredto be a plague and are to eliminate. In Indonesia, where Artificial Insemination (AI)implementation have started intensively, chromosomal aberration can be identified andculled from breeding program. This work has so far has been neglected in Indonesia.Method performed by collecting blood samples from ruminant (Madura, Buffaloand Goat) Sample of 0.5 ml of blood sample per animal was added to 5 mlchromosomal medium (Karyo MAX ^Gibco), placed in incubator at 38 oC. After 70hours, culture tube were removed from incubator, add to 1 ml working solution ofcolchicines and kept for 2 – 3 hours. The tubes were centrifuge at 1000 RPM for 10minute using PBS and supernatant was discarded, doing for 2 times respectively. Thepellet toghly packed cells added then by fixative solution. Slides were prepared bydropping the cell suspension on the glass slide and dried then stainned with Giemsastain for 10 minute. Slides were examined under high power phase-contrastmicroscope to study the chromosome spread in the single cells.Result showed that the 2N diploid number of chromosome or 3 ruminat werenormal (cattle 2N=60, Swamp buffalo 2N=50 Goat 2N=60), there were 58 autosomeand 2 sex chromosome in all animals observed. It was observed that all ruminanttested in these research were normal categories. The karyotype analysis showed thatthe chromosomes of one cell and different individual each breed varied in size, shapeand position of centromere. How ever, it was strongly recommended to performedchromosomal investigation of breeding ruminants especially for Artificial Inseminationbull purposes and others Indonesia local specific species using advanced sophisticatedtools of analysis like cytovision image analysis of fluorescent technique.Key Words: Karyotiping, Ruminant Chromosome, Abnormalities.

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