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Journal of Tropical Plant Protection
Published by Universitas Brawijaya
ISSN : -     EISSN : 23557974     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
We cordially invite you to submit a manuscript to Journal of Tropical Plant Protection (JTPP), an upcoming regular peer-reviewed open access on-line scientific journal that covers fundamental and applied aspects of plant pathology and entomology in agriculture and natural resources. The journal will consider submissions from all over the world, on research works not being published or submitted for publication as full paper, review article and research note elsewhere.
Arjuna Subject : -
Articles 3 Documents
 1 
Search results for , issue " Vol 1, No 1 (2012)" : 3 Documents clear
Molecular Characterization of a Rigid Rod-Shaped Virus Isolated from Frangipani (Plumeria sp.) Showing Mosaic Symptom in Taiwan Choliq, Fery Abdul; Chen, Tsang Hai; Sulistyowati, Liliek
Journal of Tropical Plant Protection Vol 1, No 1 (2012)
Publisher : University of Brawijaya

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Abstract

Abstract Frangipani is an important succulent plant around the worlds and also in Taiwan, for example, Plumeria rubra is widely grown as a popular ornamental tree in parks and landscaped establishments in Taiwan. Recently, a new disease in frangipani with mosaic and distortion symptoms was found in Taiwan. No viruses caused frangipani disease has been reported in Taiwan and the references about frangipani disease are still limited and only Frangipani mosaic virus (FrMV) was found. In this study, the molecular properties of a virus isolated from symptomatic frangipani in south Taiwan, such as Pingtung, Kauhsiung and Tainan were investigated. The virus with rod-shaped particles of 300 nm long and 18 nm in diameter was examined inside diseased leaves by electron microscopy. The purified virus particles showed the typical UV spectrum of tobamoviruses with A260/A280 value of 1.29 and maximum and minimum absorption at 260 nm and 249 nm, respectively. The molecular weight of19.5 kDa as the size of coat protein of tobamoviruses was estimated bysodium dedocyl sulfate-polyacrylamide gel (SDS-PAGE). Furthermore, the degenerate primers for tobamoviruses were used to amplify 568 bp and 400 bp of the DNA fragments in RT-PCR and nested PCR, respectively. Based on these results, it was confirmed that the rigidrod-shaped virus isolated from mosaic symptom of frangipani leaves is an isolate of FrMV, belonging to the genus Tobamovirus. This is the first report thatFrMV infecting Plumeria sp.in Taiwan. Keywords: Frangipani plant, mosaic disease, FrMV, Tobamovirus
Selection In vitro of Granola Potato M1 Againts Bacteria Wilt Disease Ralstonia solanacearum Asta, Hidayat; Wardiyati, Tatik; Maghfoer, Mochamad Dawam
Journal of Tropical Plant Protection Vol 1, No 1 (2012)
Publisher : University of Brawijaya

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Abstract

AbstracBacterial wilt disease caused by Ralstonia solanacearum is an important disease and could decreased yield up to 80%. Generally, bacterial disease is more difficult to control than other diseases. Moreover, there are no chemicals that can be used to control this pathogen. The approach through genetic improvement by using Ethyl Methane Sulfonate and in vitro selection are procesess to generate variants resistant against bacterial wilt on potato plants. Granola potato M1 is the result of treatment with mutagen Ethyl Methane Sulfonate. Based on the results of selection in vitro of potato plantlets M1 Granola with suspense of Rs 9 × 1010 CFU / ml, were can be induced E9 three potato plantlets are rather susceptible to  Ralstonia solanacearum through the regenerated node and E6 and E8 treatment can be induced moderately resistant planlets against Ralstonia solanacearum. Keyword: EMS, Granola, Ralstonia solanacearum
Molecular Characterization of a Rigid Rod-Shaped Virus Isolated from Frangipani (Plumeria sp.) Showing Mosaic Symptom in Taiwan Choliq, Fery Abdul; Chen, Tsang Hai; Sulistyowati, Liliek
Journal of Tropical Plant Protection Vol 1, No 1 (2012)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Frangipani is an important succulent plant around the worlds and also in Taiwan, for example, Plumeria rubra is widely grown as a popular ornamental tree in parks and landscaped establishments in Taiwan. Recently, a new disease in frangipani with mosaic and distortion symptoms was found in Taiwan. No viruses caused frangipani disease has been reported in Taiwan and the references about frangipani disease are still limited and only Frangipani mosaic virus (FrMV) was found. In this study, the molecular properties of a virus isolated from symptomatic frangipani in south Taiwan, such as Pingtung, Kauhsiung and Tainan were investigated. The virus with rod-shaped particles of 300 nm long and 18 nm in diameter was examined inside diseased leaves by electron microscopy. The purified virus particles showed the typical UV spectrum of tobamoviruses with A260/A280 value of 1.29 and maximum and minimum absorption at 260 nm and 249 nm, respectively. The molecular weight of19.5 kDa as the size of coat protein of tobamoviruses was estimated bysodium dedocyl sulfate-polyacrylamide gel (SDS-PAGE). Furthermore, the degenerate primers for tobamoviruses were used to amplify 568 bp and 400 bp of the DNA fragments in RT-PCR and nested PCR, respectively. Based on these results, it was confirmed that the rigidrod-shaped virus isolated from mosaic symptom of frangipani leaves is an isolate of FrMV, belonging to the genus Tobamovirus. This is the first report thatFrMV infecting Plumeria sp.in Taiwan. Keywords: Frangipani plant, mosaic disease, FrMV, Tobamovirus

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