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Journal of Mathematical and Fundamental Sciences
ISSN : 23375760     EISSN : 23385510     DOI : https://doi.org/10.5614/j.math.fund.sci.
Core Subject : Science, Education,
Journal of Mathematical and Fundamental Sciences welcomes full research articles in the area of Mathematics and Natural Sciences from the following subject areas: Astronomy, Chemistry, Earth Sciences (Geodesy, Geology, Geophysics, Oceanography, Meteorology), Life Sciences (Agriculture, Biochemistry, Biology, Health, Medical, Pharmacy), Mathematics, Physics, and Statistics.
Articles 3 Documents
Search results for , issue "Vol. 33 No. 3 (2001)" : 3 Documents clear
Protein yang Terkait dengan Teratogenisitas Anggota Tubuh Mencit Swiss Webster Akibat Perlakuan dengan Asam Metoksiasetat (MAA) Aceng Ruyani; Sri Sudarwati; Lien A . Sutasurya; Sony H. Sumarsono
Journal of Mathematical and Fundamental Sciences Vol. 33 No. 3 (2001)
Publisher : Institute for Research and Community Services (LPPM) ITB

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Abstract

Telah diteliti protein yang terkait dengan teratogenesis anggota tubuh mencit Swiss Webster akibat perlakuan dengan MAA. Mencit umur kebuntingan 11 hari diberi perlakuan dosis tunggal MAA 10 mmol/kg berat badan secara gavage, sedangkan kelompok kontrol hanya diberi pelarut akuabides steril. Mencit bunting dibunuh secara dislokasi leher 4 jam setelah perlakuan dengan MAA. Tunas anggota tubuh depan diisolasi dari kelompok kontrol dan perlakuan lalu dihomogenisasi. Ekstrak kasar kemudian difraksinasi dengan amonium sulfat dan masing-masing fraksi dianalisis dengan teknik l-D dan 2-D SDS-PAGE. Elektroforegram l-D dan 2-D menunjukkan bahwa pada kelompok perlakuan fraksi protein ammonium sulfat 20-40 % (F-lI), dapat dideteksi protein 31,0-36,5 kDa serta bercak protein 35,1 kDa, pI 6,2 yang tidak terdapat pada kontrol. Pada kelompok perlakuan fraksi protein ammonium sulfat 40-60% (F-lll), dapat dideteksi protein 66,3-97,4 kDa serta bercak protein 8 I,7 kDa, pl 7,3 yang tidak terdapat pada kontrol. Sedangkan pada kelompok kontrol F-llI, protein 36,5-55,4 kDa sertab ercakp rotein 41,6 kDa, pI 6,4 terdeteksi, tetapi tidak terdeteksi pada kelompok perlakuan. Dari penelitian ini dapat disimpulkan bahwa pada tunas anggota tubuh depan mencit, perlakuan dengan MAA menginduksi ekspresi dua protein(35,1 kDa, pl 6,2 dan 81,7 kDa, pl 7,3) dan menghambat ekspresi satu protein(41,6 kDa, pl 6,4). Proteins which are Linked with Swiss Webster Mouse Limb Teratogenesis as the Effects of Methoxyacetic Acid (MAA) TreatmentThe analysis of proteins, which are linked with limb teratogenesis as the effects of MAA treated in Swiss Webster mouse has been investigated. A single dose of MAA 10 mmol/kg body weight was given by gavage on gestation day 11 , whereas the control group were administered sterilized distilled water. Pregnant mice were sacrificed by cervical dislocation at 4 hours after MAA treatment. The forelimb buds were isolated from both control and treated group embryos and were then homogenized. The crude extracts were Then fractionated with ammonium sulfate and each fraction was analyzed by 1-D and 2-D SDS-PAGE techniques respectively. The l-D and 2 -D electrophoregrams revealed that in the treated group of protein fraction 20-40% ammonium sulfate ( F-II), a protein of 31.0-36.5 kDa and a protein spot 35.1 kDa, pl 6.2 could be detected, which was not found in the control. In the treated group of protein fraction 40-60% ammonium sulfate( F-lll) a protein of 66.3-97.4 kDa and a protein spot 81.7 kDa, pl 7.3 could be detected which was not found in the control, whereas in the control group a protein of 36.5-55.4 kDa, which is a protein spot4 1.6 and p l 6.4, was detected but not detected in the treated group.It could be concluded from this experiment that in the mouse forelimb buds, MAA treatment induce the protein expression of two proteins(35.1 kDa, pl 6.2 and 81.7 kDa, 7.3) and inhibit the expression of one protein (41.6p, l 6.4).
Suatu Senyawa Stilbene Terprenilasi dari Kayu Akar Tumbuhan Artocarpus Altilis Euis Holisotan Hakim; Valentina Adimurti; Lukman Makmur; Sjamsul Arifin Achmad; Didin Mujahidin; Yana Maolana Syah; Norio Aimi; Mariko Kitajima; Hiromitsu Takayama
Journal of Mathematical and Fundamental Sciences Vol. 33 No. 3 (2001)
Publisher : Institute for Research and Community Services (LPPM) ITB

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Abstract

Suatu stilben terprenilasi, trans-4-(3-metil-E-but-l-enil)-3,5,2',4'-tetrahidroksistilben telah ditemukan untuk pertama kalinya dari ekstrak kloroform kayu akar tumbuhan Artocarpus altilis (Moraceae), yang dikenal dengan nama Sukun. Struktur molekul senyawa ini telah ditetapkan berdasarkan data fisika dan spektroskopi UV, IR, MS, 1H dan 13C NMR. Senyawa ini bersifat toksik (LC50 = 45,7 µg/mL) terhadap benur udang Artemia salina Leach. A prenylated stilbene from the root trunk of Artocarpus altilisA prenylated stilbene, trans-4-(3-methyl-E-but-l-enyl)-3,5,2',4'-tetrahydroxystilbene had been isolated for the first time from the chloroform extract of the root trunk of Artocarpus altilis (Moraceae), locally known as Sukun. The structure of this compound was Elucidated based on physical and spectroscopic data UV, IR, MS, 1H and 13C NMR. It showed strong toxicity (LC50 = 45,7 µg/mL) agains Atrtemia salina Leach shrimp.*Bagian ke-25 dari seri "llmu Kimia Tumbuhan Moraceae lndonesia". Untuk Bagian ke-24 lihat pustaka (5)
Artoindonesianin-E suatu senyawa baru turunan flavanon dari tumbuhan Artocarpus Champeden Euis Holisotan Hakim; Asep Aripin; Sjamsul Arifin Achmad; Norio Aimi; Mariko Kitajima; Lukman Makmur; Didin Mujahidin; Yana Maolana Syah; Hiromitsu Takayama
Journal of Mathematical and Fundamental Sciences Vol. 33 No. 3 (2001)
Publisher : Institute for Research and Community Services (LPPM) ITB

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Abstract

Sari. Suatu senyawabaru turunan flavanon, yakni 5,7-dihidroksi-2',4',6'-trimetoksiflavanon yang diberi nama artoindonesianin-E(1 ), telah ditemukan untuk pertama kalinya bersama-samad engan artokarpin (2) dan heteroflavanon-A (3) pada ekstrak benzena kulit batang tumbuhan Artocarpus champeden Spreng (Moraceae), suatu tumbuhan yang endemik untuk Indonesia, serta dikenal dengan narna Cempedak. Struktur molekul senyawa tersebut telah ditetapkan berdasarkan data fisika dan spekroskopi (MS, 1H dan 13C NMR).Artoindonesianin-E a New Flavanone Derivative from Artocarpus ChampedenAbstract. A new flavanone derivative, 5,7-dihydroxy-2',4',6'-trimethoxyflavanone named artoindonesianin-E (1) had been isolated for the first time, together with artocarpin (2) and heteroflavanone-A (3) from benzene extract of the tree bark of Artocarpus champeden Spreng. (Moraceae), a species endemic to Indonesia and locally known as Cempedak. The structure of this compound was elucidated based on physical and spectroscopic data (MS, 1H and 13C NMR).

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