cover
Contact Name
Rachma Wikandari
Contact Email
rachma_wikandari@mail.ugm.ac.id
Phone
+6285712601130
Journal Mail Official
agritech@ugm.ac.id
Editorial Address
Faculty of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora No. 1, Bulaksumur, Yogyakarta 55281, Indonesia
Location
Kab. sleman,
Daerah istimewa yogyakarta
INDONESIA
agriTECH
ISSN : 02160455     EISSN : 25273825     DOI : 10.22146/agritech
Core Subject : Agriculture,
Agritech with registered number ISSN 0216-0455 (print) and ISSN 2527-3825 (online) is a scientific journal that publishes the results of research in the field of food and agricultural product technology, agricultural and bio-system engineering, and agroindustrial technology. This journal is published by Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta in colaboration with Indonesian Association of Food Technologies (PATPI).
Articles 7 Documents
Search results for , issue "Vol 28, No 2 (2008)" : 7 Documents clear
Potensi Isolat Khamir H10 sebagai Agensia Biologis untuk Pengendalian Mucor piriformis pada Buah Apel Sri Widyastuti
agriTECH Vol 28, No 2 (2008)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (896.862 KB) | DOI: 10.22146/agritech.9861

Abstract

The potency of the yeast cell isolate H10 in suppressing post-harvest rot, Mucor piriformis, was studied in the wounds of apple fruit. Spore inoculum at diffierent concentrations were inoculated into the artificially-made wounds of fresh picking apple fruit. There were five replicates of single fruit with 4 wounds each. Fruits were incubated at 20 °C for 4 days. Increasing concentration of the pathogen spores applied in the wounds, produced higher percentage of rot and diameter of lesion. At the concentration of 107 and 108 cfu ml-1, the yeast cells were able to suppress the percentage of infection and diameter of lesion caused by the pathogen up to 65 %. The inhibition activity of the yeast cells was most highest when the yeast applied coincidentally or 2 hours before inoculation of the pathogen inoculum. These evidences indicate that the yeast is potential to be developed as a protective bio-control agent to suppress the development of post-harvest diseases of apple caused by M. piriformis.ABSTRAKPotensi sel khamir isolat H10 untuk menghambat perkembangan jamur patogen pasca panen Mucor piriformis dipela- jari pada luka buatan pada buah apel. Larutan spora pada berbagai konsentrasi diinokulasikan ke dalam luka buatan pada buah apel segar. Setiap perlakuan menggunakan lima buah apel yang masing-masing mempunyai 4 buah luka. Buah kemudian disimpan untuk dihitung persentase infeksi dan diameter luka yang terjadi selama 4 hari penyimpanan pada suhu 20 °C. Meningkatnya konsentrasi spora patogen yang diinokulasikan pada luka, meningkat pula persentase infeksi dan diameter luka. Sel khamir terbukti mampu menggandakan diri pada permukaan jaringan luka. Pada kon- sentrasi 107  dan 108  cfu ml-1, sel khamir dapat secara nyata menghambat perkembangan jamur patogen sampai 65%. Penghambatan tersebut lebih efektif pada sel khamir yang diaplikasikan bersamaan dengan atau 2 jam sebelum inokulasi inokulum spora pathogen. Efektifitas penghambatan pada buah segar lebih tinggi dibanding pada buah yang telah disimpan beberapa lama. Kenyataan tersebut mengisyaratkan bahwa isolat khamir H10 mempunyai potensi dapat dikembangkan menjadi agensia biologis yang bersifat protektif untuk pengendalian penyakit pasca panen buah apel yang disebabkan oleh M. piriformis.
Potensi Protein Kecambah Kedelai dalam Menstimulasi Sekresi Insulin pada Pankreas Tikus Normal dan Diabetes Bayu Kanetro; Zuheid Noor; Sutardi Sutardi; Retno Indrati
agriTECH Vol 28, No 2 (2008)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6261.511 KB) | DOI: 10.22146/agritech.9862

Abstract

The objective of this investigation was to examine the potential of seed and germinated SP that were extracted at two condition precipitation, Kunitz Trypsin Inhibitor (KTI) and Bowman Birk Inhibitor (BBI) to stimulate insulin secretion of the pancreas of induced diabetic and normal rats by in vitro bioassay. Mature male Sprague Dawley (SD) rats of the in vitro bioassay were divided into two groups. They were diabetic rats that was induced by aloksan injection and normal rats (without aloksan injection). Rat was anaesthetized with ether, and the pancreas was taken out, injected by HBS solution, hydrolyzed by collagenase and then washed several times with RPMI 1640 LITE solution and Kreb Ringer bicarbonate buffer before incubation. The islets of the pancreas were incubated in Kreb buffer under seven conditions of media treatment as follows: glucose medium that was used as a reference standard (R), total protein of seed (SPT), total protein of germinated soybean (GPT), TI protein of seed (SPTI), TI protein of germinated soybean (GPTI), KTI, and BBI. After two hours of incubation, the mixture was sonicated, and centrifuged The supernatant was stored at –20 oC for determination of insulin. The insulin analysis was conducted by ELISA method. The results show that aloksan injection increased the level of blood sugar and induced diabetic rats. The media treatment altered the soluble protein profile and the insulin secretion of the islets. The germinated SP has the ability to stimulate  insulin secretion of the pancreas of  diabetic as well as normal rats. The insulin secretion of the islets in the medium of crude TI of germinated soybean (GPTI) was highest. The potential order of the insulin secretion of normal rats islets were GPTI, KTI, BBI, GPT, SPTI, SPT, and R media. While the potential order of the insulin secretion of diabetic rat islets were GPTI, GPT, SPTI, BBI, KTI, SPT, and R media. The potential of germinated SP to stimulate insulin secretion was better than ungerminated SP.ABSTRAKTujuan dari penelitian ini adalah mempelajari potensi protein biji dan kecambah kedelai (protein total dan TI), Kunitz Trypsin Inhbitor (KTI), dan Bowman Birk Inhibitor (BBI)  dalam menstimulasi sekresi insulin pada pankreas tikus normal dan diabetes melalui pengujian biologis secara in vitro. Tikus Sprague Dawley (SD) jantan yang diguna- kan dalam pengujian biologis secara in vitro dibagi menjadi dua kelompok, yaitu tikus diabetes yang disiapkan dengan cara menginduksi melalui injeksi aloksan, dan tikus normal (tanpa diinjeksi aloksan). Selanjutnya tikus dieksekusi dengan bius ether dan dilakukan pencucian beberapa kali dengan larutan RPMI 1640 LITE. Pencucian terakhir meng- gunakan buffer Kreb Ringer Bicarbonat sebelum pancreas islet yang diperoleh diinkubasi. Pancreas islet diinkubasi dalam Kreb buffer yang dicampur dengan 7 perlakuan media inkubasi yang berbeda, yaitu medium glukosa sebagai control (R), protein total biji kedelai (SPT), protein total kecambah kedelai (GPT), protein TI biji kedelai (SPTI), pro- tein TI kecambah kedelai (GPTI), KTI dan BBI. Inkubasi dilakukan selama 2 jam, kemudian disonikasi, dan sentrifu- gasi. Supernatan yang diperoleh disimpan pada suhu -20 oC untuk disiapkan dalam  analisis insulin mengggunakan metode ELISA. Hasil penelitian menunjukkan bahwa injeksi aloksan pada tikus dapat menginduksi terjadinya diabetes karena dapat meningkatkan gula darah tikus. Perlakuan media inkubasi berpengaruh terhadap kadar protein terlarut media dan kemampuannya menstimulasi islet untuk mensekresikan insulin. Protein kecambah kedelai memiliki kemam- puan menstimulasi sekresi insulin baik pada pancreas tikus normal maupun diabetes. Sekresi insulin dari islet dalam medium GPTI paling tinggi dibandingkan perlauan yang lain. Urutan potensi sekresi insulin dari pancreas tikus nor- mal, yaitu berturut-turut dari yang tertinggi GPTI, KTI, BBI, GPT, SPTI, SPT, dan R. Sedangkan pada pancreas tikus diabetes, yaitu dari yang tertinggi GPTI, GPT, SPTI, BBI, KTI, SPT, and R media. Secara umum disimpulkan bahwa potensi protein kecambah kedelai dalam menstimulasi sekresi insulin lebih baik dibandingkan protein biji kedelai.
Study of Household Biodigesters Chandra Wahyu Purnomo; Ambar Pertiwiningrum; M. Prasetya Kurniawan
agriTECH Vol 28, No 2 (2008)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6260.585 KB) | DOI: 10.22146/agritech.9863

Abstract

Tunnel or tubular type of bio-digester has been widely developed in many countries for producing a renewable clean fuel gas named biogas. This digester type only requires minimum maintenance and simple construction for ensuring its lower cost compared to others with similar capacity. Mostly, polyethylene (PE) plastic bag is used for construction materials of the tubular digesters. In this research, the tubular type has been constructed using large diameter PVC (Poly Vinyl Chloride) pipe for increasing the durability and safety. The performance of PVC digesters have been tested by measuring biogas production rate and CH concentration in the gas. COD (Chemical Oxygen Demand) and BOD (Biological Oxygen Demand) drop between inlet and outlet sludge have been measured for examining the digester ef- ficiency. Then, the performance was compared with PE plastic digesters data from several literatures which still needs some improvements for enhancing its capability and performance.
Efek pH dan Konsentrasi Butirat Anhidrida selama Butirilisasi Pati Garut Damat Damat; Haryadi Haryadi; Y. Marsono; Muhammad Nur Cahyanto
agriTECH Vol 28, No 2 (2008)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6261.389 KB) | DOI: 10.22146/agritech.9864

Abstract

Several characteristics of native starch have limited utilizations. Chemical modification improves the physical, chemi- cal, and functional properties of starch and therefore it extends the utilization. The aim of this research was to inves- tigate the effect of the dispersion of pH (8, 10 and 12) and butyrate anhydride concentration (5, 10, 15, 20 and 25 %) on the butyrilization process of arrowroot starch.  The modified (butyrated)  starch was analyzed for its butyril group, degree of substitution (DS), swelling power, solubility, amylography, and transmittance. The result indicated that the maximum butyriilation was achieved at pH 10 and addition of 20 % butyrate anhydride. The higher DS resulted in more transparent starch paste, the more stable viscosity and the higher swelling power.ABSTRAKPati garut, sebagaimana jenis pati alami lainnya diketahui memiliki kelemahan sifat fisik dan kimia yang menyebabkan penggunaan pati garut pada industri pangan relatif terbatas. Untuk itu maka perlu dilakukan perbaikan sifat fisik dan kimianya dengan cara melakukan modifikasi pati garut. Penelitian ini dilakukan dengan tujuan untuk mendapatkan pengaruh pH dispersi (8, 10 dan 12) dan konsentrasi butirat anhidrida (5, 10, 15, 20 dan 25 %) pada proses butirilisasi pati garut.   Selain itu, penelitian ini juga dilakukan untuk mengetahui karakteristik pati-garut butirat, yang meli- puti persen butiril, derajat substitusi, daya mengembang, kelarutan, sifat amilografi dan kejernihan pasta pati-garut butirat. Hasil penelitian menunjukkan bahwa butirilisasi maksimum dicapai pada pH 10 dengan konsentrasi butirat anhidrida 20 %. Pati butirat derajat substitusi yang tinggi memiliki kejernihan pasta, daya mengembang yang lebih tinggi dan viskositas yang lebih stabil, sedangkan kelarutan lebih rendah.
Pengaruh Varietas Apel dan Campuran Bakteri Asam Asetat terhadap Proses Fermentasi Cider Dessi Caturryanti; Sri Luwihana; Siti Tamaroh
agriTECH Vol 28, No 2 (2008)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6260.759 KB) | DOI: 10.22146/agritech.9865

Abstract

The objectives of the research were evaluate apple variety and acetic acid bacteria ratio used in cider fermentation. Two apple varieties (Manalagi and Rome Beauty) were used and mixed culture of two bacteria species (Acetobacter pasteurianus INT-7 and Acetobacter aceti JCM 7640) were used for inoculation. Apple extract was inoculated by ratio of acetic bacteria 1:1 and 1:2, and etanol substrat of 5 % was added respectivelly, continued aerobic condition at room temperature for 7 days. The result of the reseach indicated that cider fermentation using Rome Beauty variety with mixed culture of A. pasteurianus INT-7 : A. aceti JCM 7640 = 1:2 produced acetic acid 3.11 %, product yield 0.85 g/g and efficiency 60.56 %.ABSTRAKTujuan penelitian ini adalah untuk mengevaluasi pearan varietas apel dan rasio bakteri asam asetat dalam fermen- tasi cider. Dua varietas apel (Manalagi and Rome Beauty) dan dua kultur bakteri (Acetobacter pasteurianus INT-7 and Acetobacter aceti JCM 7640) digunakan dalam penelitian ini. Ekstrak buah apel dinokulasi dengan bakteri asam asetat dengan rasio 1:1 dan 1:2. Sebagai substrat ditambahkan ke dalam medium fermentasi etanol sebanyak 5 %. Fermentasi silakukan secara aerobik pada suhu ruang selama 7 hari. Hasil penelitian menunjukkan bahwa fermentasi cider menggunakan varietas apel Rome Beauty dengan perbandingan A. pasteurianus : A. aceti = 1:2 menghasilkan asam asetat sebanyak 3,11 %, yield produk 0,85 dan efisiensi sebesar 60,56 %.
Komponen Kimia dan Anatomi Tiga Jenis Bambu dari Seram, Maluku Mery Loiwatu; Elyazar Manuhuwa
agriTECH Vol 28, No 2 (2008)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6262.436 KB) | DOI: 10.22146/agritech.9866

Abstract

As a versatile plant, the basic properties of bamboo are various, depending on species, site, and position inside the stem. This study was conducted at three sites (Buria, Morekao, Tala), on three species (Dendrocalamus asper, Schizos- tachyium brachycladum, Schyzotachium lima), and three positions of the stem (base, middle, top). Objective of the study was to measure chemical component and anatomical feature of the three species of bamboos of West Ceram’s Sub-district (Taniwel, Piru, Kairatu) and three parts of stem (base, middle, top). Factorial experiment in split plot design was applied in the study with 3 replications. Result of the study indicated that there was a significant effect of location on the lignin content, diameter of the fiber and fiber cells lumen, and proportion of parenchyma cells; species of bamboo to the length and diameter of fiber cells; and portion of stem to the extractive soluble in hot water, soluble in alcohol benzene, length and diameter of fiber cells, and diameter of fiber lumens. Extractive soluble in cold water was 3.10-3.79 %; hot water was 5.43-6.23 %; alcohol benzene was 3.37-4.10 %; alpha cellulose was 44.22-46,94 %; holocellulose was 71.97-75.57 %; lignin was 26.00-27.37 %; length of fiber cell was 3.40-3,96 mm; diameter of fiber cell was 4.34-4.91 micron; diameter of fiber cell lumens was 2.74-3.23 micron; cell wall thickness of fiber cell was0.76-0.91 micron; proportion of parenchyma cells was 51.95-56.85 %; proportion of fiber cells was 27.81-62.66 %;and proportion of pores was 12.39-14.60 %.ABSTRAKSebagai tanaman serbaguna, sifat dasar bambu bervariasi menurut jenis, tempat tumbuh dan posisi didalam batang. Penelitian dilakukan pada 3 lokasi (Buria, Morekao, Tala), terhadap 3 jenis bambu (Dendrocalamus asper, Schizos- tachyium brachycladum, Schyzotachium lima), dan 3 posisi dalam batang (pangkal, tengah dan ujung). Tujuan pene- litian adalah untuk mengukur komponen kimia dan anatomi 3 jenis bambu (Dendrocalamus asper, Schizostachyium brachycladum, Schyzotachium lima) asal 3 kecamatan Seram bagian barat (Taniwel, Piru, Kairatu), dan 3 bagian batang (pangkal, tengah dan ujung). Percobaan faktorial dalam rancangan petak terpisah digunakan dalam penelitian dengan 3 ulangan. Hasil penelitian menunjukkan pengaruh signifikan lokasi terhadap kadar lignin, diameter sel serat dan rongga sel serat dan proporsi sel parensim; jenis bambu terhadap panjang dan diameter sel serat; posisi dalam batang terhadap kadar ekstraktif larut air dingin, ekstraktif larut alkohol benzen, panjang dan diameter sel serat, dan diameter sel serat. Ekstraktif larut air dingin berkisar 3,10-3,79 %; larut air panas 5,43-6,23 %; larut alkohol benzen3,37-4,10 %; alfa selulosa 44,22-46,94 %; holoselulosa 71,97-75,57 %; lignin 26,00-27,37 %; panjang sel serat 3,40-3,96 mm; diameter sel serat 4,34-4,91 mikron; diameter rongga sel serat 2,74-3,23 mikron; tebal dinding sel serat0,76-0,91 mikron proporsi sel parensim 51,95-56,85 %; proporsi sel sel serat 27,81-62,66 %; dan proporsi sel pori12,39-14,60 %.
Aktivitas Antioksidasi Ekstrak Fenol Umbi Sarang Semut (HYDNOPHYTUM SP.) Pada berbagai Suhu Penyeduhan Yohana S.K. Dewi; Dominika Dominika
agriTECH Vol 28, No 2 (2008)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6330.664 KB) | DOI: 10.22146/agritech.9867

Abstract

Antioxidant activity of Sarang Semut tubers (Hydnophytum sp.) extracts toward stepping treatments was investigated. Powder of sarang semut tubers was diluted in 200 ml of distilled water then its being subjected to stepping treatment of three different treatments: cooked at 60, 80 and 100 °C for 2, 4 and 6 minutes. Effect of stepping treatment of Sarang Semut tuber juices on the antioxidant activity and the total phenol contents was investigated. Antioxidant activity of Sarang Semut tuber juices was estimated according to DPPH methods. The total phenol content in the tuber juices was measured with Folin-Ciocalteu reagent using gallic acid as a standard. The results showed that stepping treatment did not affect antioxidant activity of Sarang Semut tuber juices. Stepping treatment of Sarang Semut tuber juices at 100 °C exhibited the highest total phenol contents.ABSTRAKAktivitas antioksidasi ekstrak umbi sarang semut (Hydnophytum sp.) telah diteliti pada berbagai suhu penyeduhan. Serbuk umbi sarang semut (2,5g) diseduh dalam akuades yang telah dipanaskan pada suhu 60,8 dan 100 °C selama 2,4, dan 6 menit. Pengaruh perlakuan penyeduhan terhadap serbuk sarang semut diuji aktivitas antioksidasi dan kand- ungan total fenol. Pengujian aktivitas antioksidasi dengan metode DPPH dan kandungan total fenol dengan metode Folin Ciocalteou dengan standar asam galat. Hasil penelitian menunjukkan perlakuan penyeduhan tidak mempengar- uhi aktivitas antioksidasi serbuk sarang semut. Suhu penyeduhan 100 °C menghasilkan kandungan total fenol umbi sarng semut tertinggi.

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