Garuda - Garba Rujukan Digital

p-Index From 2020 - 2025

0.888

P-Index

This Author published in this journals

All Journal Jurnal Teknologi Dan Industri Pangan Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Natur Indonesia Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Majalah Kulit, Karet, dan Plastik agriTECH Indonesian Food and Nutrition Progress Biosaintifika: Journal of Biology & Biology Education Jurnal Pengelolaan Laboratorium Pendidikan Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan

Indrati, Retno

Department Of Food And Agricultural Product Technology, Faculty Of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora No.1 Bulaksumur, Yogyakarta, Indonesia 55281

Author-ID : 2869793

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Engineering Environmental Science Health Professions Industrial & Manufacturing Engineering Medicine & Pharmacology Public Health

Published : 31 Documents Claim Missing Document

Claim Missing Document

Articles

1 2 3 4

Aktivitas enzim lipase alkali dari bakteri dalam surfaktan Rumiyati, V. Sri Pertiwi; Indrati, Retno
Majalah Kulit, Karet, dan Plastik Vol 14, No 26 (1999): Majalah Barang Kulit, Karet, dan Plastik
Publisher : Center for Leather, Rubber, and Plastic Ministry of Industry, Indonesia

Activity and stability of alkaline lipase from bacteria (strain AS, KB andÂ SP) were studied in containing of surfactants at 0,05% and 0,10%. The survactanswere used in research, these were consisted of four anionic surfactants (cetyl trimethyl ammonium bromide, cetyl pyridium chloride, cetyl dimethyl ammonium bromide) and there nonionic surfactants (Triton X â 100, tergitol, and nonidet P.40). Production of enzyme was produced in initial medium pH 7,5; incubation at 37oC for 48 h. These research showed that activity of alkaline lipase from strain A S-1; AS-2; KB-4; SP-2Â and SP-13Â was stable in containing of anionic surfactants and nonionic surfactants at 0,05% and alkaline lipase from KB-8Â and SP-1 were showed decrease of activity (relatif activity < 80 %). Strain KB â 4 was prodused alkalineÂ lipase which stable in containing of anionic surfactants, cationic surfactants and nonionic surfactants at 0,05% & 0,10%. It was had high activity (activity relatif 90-125%).Â Â INTISARIÂ Aktivitas dan stabilitas enzim lipase alkali dari bakteri yang berasal dari isolat AS, KB dan SP dipelajari dalam kondisi yang mengandung surfaktan dengan konsentrasi akhir 0,05% dan 0,10%. Surfaktan yang digunakan dalam penelitian adalah empat jenis surfaktan anionik (SDS, niaproof, n-lauroyl sarcosine, dehydrocholic acid), tiga jenis surfaktan kationik (cetyl trimethyl ammonium bromide, cetyl pyridium chloride, cethyl diemthyl ammonium bromida) dan tiga jenis surfaktat nonionik (triton X â 100, tergitol, dan nonidet P -40). Produksi enzim lipase dilaksanakan pada media dengan pH awal 7,5 , suhu inkubasi 37oC selama 48 jam. Hasil penelitian menunjukkan bahwa aktivitasÂ Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â enzimÂ lipase alkali yang berasal dari isolat AS-1, AS-2, KB-4, SP-2 dan SP-13Â relatif stabil pada kondisi yang mengandung surfaktan anionik, kationik dan non ionik 0,05%,Â dan yang berasal dari KB-8Â dan SP-1 aktivitasnya menurun (aktivitasÂ relatif < 80%). Isolat KB â 4 memproduksi enzim lipase alkali stabil pada kondisi yang mengandung surfaktan anionik, kationik dan non ionik 0,05% dan 0,10%. Enzim tersebut mempunyai aktivitasÂ relatif tinggi (90-125%).

Effect of Lime Pretreatment on Microstructure of Cassava Stalk Fibers and Growth of Aspergillus niger Dewi, Pramesti; Indrati, Retno; Millati, Ria; Sardjono, Sardjono
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.13802

Cassava stalk can be converted into sugar-based product by using microorganism. Unfortunately, lignin act as a barrier of optimal bioconversion. Cassava stalk needs pretreatment process for removing this barrier. The effect of lime pretreatment on microstructure of cassava stalk fibers and the growth of Aspergillus niger FNCC 6114 were observed in this research. The cassava stalks were reduced into 0.147- 0.297 mm size and pretreated with 1 % Ca(OH)2. Lime pretreated and unpretreated cassava stalk was used as solid medium for Aspegillus niger FNCC 6114. The effect of pretreatment method on fibers microstructure of cassava stalk was evaluated through SEM micrograph. The growth and metabolism activities of Aspergillus niger FNCC 6114 were monitored through SEM micrograph of media after fermentation. The other parameters examined were changes in glucosamine, reducing sugar levels, and sporesâ quantity. Lime pretreatment altered microstructure of cassava stalk fibers. However, cassava stalk without lime pretreatment gave better growth of Aspergillus niger FNCC 6144 based on metabolism activities parameters. Cassava stalks is suitable as media for Aspergillus niger FNCC 6144 through solid state fermentation. For better growth of Aspergillus niger FNCC 6144 fine-sized cassava stalk should not be lime pretreated. The results of this studyÂ provideÂ information about the pretreatment of cassava stems which was effective in supporting the growth of Aspergillus niger. Enhancements the utilization of cassava stems by using fungi, for example Aspergillus niger can overcome the accumulation of organic waste that can interfere with environmental sustainability.

Isolasi dan Identifikasi Bakteri Asam Laktat Proteolitik dari Susu Kedelai yang Terfermentasi Spontan Yusmarini, Yusmarini; Indrati, Retno; Utami, Tyas; Marsono, Yustinus
Jurnal Natur Indonesia Vol 12, No 1 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Lactic acid bacteria is a group of bacteria with proteolytic activities enambling to grow on protein rich substratesuch as soymilk. This research was aim to isolate and identify lactic acid bacteria with have proteolytic activityfrom spontaneous fermented soy milk. Sixteen isolates out of 26 colonies isolated from fermented soymilk arepresumed as lactic acid bacteria. Among these 16 isolates, only 3 of them showed proteolytic activity. These threeisolates were further identify morphologically and only two isolates identified as Lactic Acid Bacteria, namelyR.1.3.2 and R.11.1.2. The ability of these isolates to produce acid and protease were observed. The results showedthat isolate R 1.2.3 higher ability in producing protease.

Aktivitas Trypsin Inhibitor Berbagai Varietas Biji Kedelai (Glycine max L.) dan Perubahannya Selama Perkecambahan Biji dari Varietas Terbaik Kanetro, Bayu; Noor, Zuheid; Indrati, Retno
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 1 (2007): February 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v12i1.2529

This study investigated the activity of trypsin inhibitor (TI) of some soybean (Glycine max L.) varieties and the change in TI activity during seed germination of the best variety. The research was aimed to determine the best variety of soybean and germination time based on the highest TI activity. There were 5 varieties of soybeans, Paderman, Argomulyo, Kaba, Sinabung, and Ijen. The best variety of soybean was Sinabung as shown by the highest TI activity. The soybean of Sinabung variety was germinated for 6 various germination times at 12, 24, 36, 48, 60 and 72 hr. The result showed that the variation of germination time changed TI activity. TI activity decreased significantly after 36 hr of germination of soybeans. The best time of germination was 36 hr.

A Kinetic Study of Xylitol Production with Glucose and Arabinose as Cosubstrate by Candida shehatae WAY 08 Wisnu Adi Yulianto; Kapti Rahayu Kuswanto; Tranggono .; Retno Indrati
Jurnal Teknologi dan Industri Pangan Vol. 16 No. 3 (2005): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2969.697 KB)

Xylitol production by Candida shehatae WAY 08 was investigated under two sets of conditions (a) with addition of glucose or arabinose as cosubstrate, (b) ratio of xylose to cosubstrate at the range of 6:1Ã¢â&sbquo;¬â€ 6:3%. The fermentation was performed at 3000/n 500 ml Erlenmeyer flasks placed in a shaker incubator at 200 rpm for 72 h. Biornass concentration was calculated as dry mass. Xylose. cosubstrate. xylitol, ethanol, and acetic acid concentrator ware determined using API. C. The result indicated that addition of arabinose as cosubstrate to xylose within the ratio range of 1:6Ã¢â&sbquo;¬â€3:6% could increase xylitol production. The highest xylitol yield (0,84 gIg) and volumetric rate of xylitol production (0.66 g/Lh) were achieved at ratio of xylose to arabinose as high as 6:1%. However, addition of glucose as cosubstrate decreased xylitul production. A medium containing 6% glucose as a sole carbon source could achieve the hiqhest ethanol yield(0.32 g/g) and growth yiÃ&fnof;Â¨ld (0,21/ gIg). wh;/e arabinose as a sole source was metabolized mainly for biomoss formation. Key words xylose. cosubstrate. arabinose, glucose. xylitoi. Candida shehatae WAY 08.

PENGIKATAN GARAM EMPEDU OLEH SUSU KEDELAI TERFERMENTASI DAN STABILITASNYA TERHADAP PEPSIN DAN PANKREATIN [Binding of Bile Salts by Fermented Soymilk and Its Stability Against Pepsin and Pancreatin] . Yusmarini; Retno Indrati; Tyas Utami; Yustinus Marsono
Jurnal Teknologi dan Industri Pangan Vol. 24 No. 1 (2013): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

ABSTRACT Processed soybean products especially the fermented ones have beneficial health effects since they are capable of reducing the level of plasmacholesterol (hypocholesterolemic effect). One of the mechanisms is by increasing the binding of bile salt. This research was aimed to assess the ability of soymilk, fermented soymilk products and fermented soymilk products combined with enzymatic hydrolysis to bind bile salts. The stability of the binding against hydrolysis by digestive enzymes (pepsin and pancreatin) was also evaluated. Fermented soybean products inoculated with isolates of L. plantarum 1 R.11.1.2 was be able to bind 1.40 μmol/100 mg protein (62.26%) of natrium taurocholate. This binding ability is slightly higher than that of soymilk to natrium taurocholate, i.e.1.33 μmol/100 mg protein (59.04%). Addition of a protease enzyme specific to hydrophobic amino acid (thermolysin) on fermented soymilk products was able to enhance the ability of bind natrium taurocholate. Enzymatic hydrolysis products having a molecular weight of

Extraction and characterization of acid-soluble collagen and pepsin-soluble collagen from the dry scales of the striped snakehead (Channa striatus) Bagus Fajar Pamungkas; Supriyadi Supriyadi; Agnes Murdiati; Retno Indrati
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 21 No 3 (2018): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Characteristics of collagen are influenced by the source of raw materials and extraction methods used. The aim of this research was to characterize the acid- and pepsin-soluble collagens from the dry scales of the striped snakehead (Channa striatus). Collagen was extracted using to methods including 0.5 M acetic acid and 0.1% pepsin. The yield of acid soluble collagen (KLA-SH) and pepsin soluble collagen (KLP-SH) were 0.98% and 1.94%, respectively. KLA-SH and KLP-SH contained glycine as the major amino acid and had high imino acid group content i.e 226 and 230 residues/1.000 residues, respectively. FTIR spectra of KLA-SH and KLP-SH showed that of the structure of collagen could be maintained in the form of triple helix structure. KLA-SH and KLP-SH consisted of α1- and α2-chain, β-chain, and γ-chain and is suggested as type I collagen.

Karakteristik Trypsin Inhibitor dan Penjajagan Sebagai Komponen Makanan Fungsional Penderita Diabetes (IIDM) Bayu Kanetro; Zuheid Noor; Sutardi Sutardi; Retno Indrati
agriTECH Vol 25, No 4 (2005)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Trypsin inhibitors (TI) are substances which, when added to a mixture of a trypsin and a subtrate, bind to the enzyme and render it to decrease in the rate of substrate cleavage. These inhibitors are nonglycosylated, water-soluble (albumin) that account for about 0,2-2 % of the total soluble protein of the legume seeds. Two major families of trypsin inhibitors have been described in legumes: the Bowmen-Brix-type (BBI) and the Kunitz-type inhibitors (KTI). They are distinct families of proteins, as evidenced by their molecular weights, compositions, and amino acid sequences. Because of their unique pharmacological properties, these inhibitors hold considerable promise in clinical applications in their field of medicine. At least one inhibitor in soybeans, the BM, has been shown to have clear anticarcinogenic activity in both in vitro and in vivo carcinogenic Trypsin inhbitors (TI) are substances which, when added to a mixture of a trypsin and a substrate, bind to the enzyme and render it to decrease in the rate of substrate cleavage. These inhibitors are nonglycosylated, water-soluble (albumin) that account for about 0,2 â€“2 % of the total soluble protein of the legume seeds. Two major families of trypsin inhibitors have been described in legumes: the Bowmen-Birk-type (BBI) and the Kunitz-type inhibitors (K7'I). They are distinct families of proteins, as evidenced by their molecular weights, compositions, and amino acid sequences. Because of their unique pharmacological assay systems. increasing need and awareness for functional food has motivated food scientists and industries to search functional food components and ingredients for certain target group, including functional food for diabetes. Soy TI may enhance the production of more trypsin and probably insulin as well. The dietary 77evokes pancreatic enzyme secretion by forming inactive trypsinT1 complex. As the level of trypsin goes below a threshold level, the pancreas is induced to produce more enzymes. The mediating agent between the enzymes and the pancreas is cholecystokinin (CCK), which is released from the jejunul endocrine cells when the level of trypsin in the intestine becomes depleted. CCK is intestinal hormone which stimulate insulin secretion. Ti regenerated 13-cells which indicate the beneficial effect of TI on the insulin production of the pancreas. These novel findings provide evidence to support the potential utility of TI in the treatment of type 2 diabetes (11DM/ Insulin-Independent Diabetic Mellitus). However, TI are also known as anti nutrient substances and to cause pancreatic hypertrophy.

Perbandingan Karakteristik Kimia Trypsin Inhbibitor Biji dan Kecambah Kedelai Bayu Kanetro; Zuheid Noor; Sutardi Sutardi; Retno Indrati
agriTECH Vol 26, No 4 (2006)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

This study investigated the changes in trypsin inhibitor (TI) activity, amino acid composition and protein patterns after 36 hours germination of soybeans. TI was extracted by a combination of pH precipitation and centrifugation. TI activity of seeds and germinated soybean were 1.64 and 1.68 IU/mg seeds (db) respectively. Heating of crude TI decreased heat stability of TI. The decrease of TI activity of germinated soybeans due to heating was lower than seeds. The amino acid composition and protein patterns of soybeans changed after 36 hours germination. Thr, Ala, Trp, Met and Lys decreased significantly, while Asp, Glu, Val, Phe, and Ile increased significantly. The SDS-PAGE analysis showed that the seeds contain high-molecular weight of proteins which were degraded after 36 hours germination result in smaller molecular weight of proteins.

Isolation of Rhizopus oryzae From Rotten Fruit and Its Potency For Lactic Acid Production in Glucose Medium with and without Addition of Calcium Carbonate Titik Kurniawati; Retno Indrati; Sardjono Sardjono
agriTECH Vol 34, No 2 (2014)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Studies on lactic acid production by filamentous fungi Rhizopus oryzae have been explored in the world. Unfortunately, these studies are still limited in Indonesia, particularly studies in lactic acid production by indigenous strain R. oryzae. Four strains obtained from rotten avocado and guava were potential in producing lactic acid (AT1, JT1, AT2, and AT3). Rhizopusoryzae AT3 was used for lactic acid production using 100 g/l glucose medium with and without addition of 7.5 g/l calcium carbonate (CaCO3) at initial fermentation. Addition of CaCO3 increased lactic acid concentration of 59.30%, the concentrations were 11.61 g/l and 18.495 g/l in glucose medium and glucose medium with CaCO3 addition, respectively. Glucose+CaCO3 medium also showed higher productivity, reached continuously from 1 day (0.059 g/l/h) until 5 days fermentation (0.154 g/l/h), whereas highest productivity in glucose medium was reached at 1 day fermentation (0.124 g/l/h) and continued to decrease until 5 days fermentation (0.065 g/l/h).

Co-Authors Agnes Murdiati Bagus Fajar Pamungkas Bayu Kanetro Bayu Kanetro Bayu Kanetro Djagal W. Marseno Djagal Wiseso Marseno Djagal Wiseso Marseno Djagal Wiseso Marseno Endang S. Rahayu Hari Eko Irianto I Dewa Gde Mayun Permana Kapti Rahayu Kuswanto Kapti Rahayu Kuswanto Maria Ulfah Marta Tika Handayani Marwati Mary Astuty Masahiro Ogawa Muhammad Halim Muhammad Nur Cahyanto Ningsih, Dewi Pujo Nurin Imana Hidayati Pramesti Dewi Pudji Hastuti Pudji Hastuti Purnama Darmadji Purnomo Darmadji Rachma, Yasmin Aulia Rahayu, Novia Aristi Ria Millati Sardjono Sardjono Sardjono Sardjono Sardjono Sardjono Sardjono Sardjono Setyabudi, Francis M.C. Sigit Sigit Setyabudi Suci Apsari Pebrianti Suparmo Suparmo Supriyadi Supriyadi Supriyadi Supriyadi Suryanti Suryanti Suryanti Suryanti Sutardi Sutardi Sutardi Sutardi Sutardi Sutardi Titik Kurniawati Tranggono . Tranggono Tranggono Tyas Utami Tyas Utami V. Sri Pertiwi Rumiyati, V. Sri Pertiwi V.S. Pertiwi Rumiyati Widyahapsari, Dhina Aprilia Nurani Wisnu Adi Yulianto Yastanto, Anang Juni Yoshiyuka Ohta Yotty Atmajaya Yuliana, Eva Yusmarini Yusmarini Yustinus Marsono Yustinus Marsono Zuheid Noor Zuheid Noor Zuheid Noor Zuheid Noor Zulafa Noor

Title

Found 31 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 31 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 31 Documents
Search