cover
Contact Name
Rachma Wikandari
Contact Email
rachma_wikandari@mail.ugm.ac.id
Phone
+6285712601130
Journal Mail Official
agritech@ugm.ac.id
Editorial Address
Faculty of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora No. 1, Bulaksumur, Yogyakarta 55281, Indonesia
Location
Kab. sleman,
Daerah istimewa yogyakarta
INDONESIA
agriTECH
ISSN : 02160455     EISSN : 25273825     DOI : 10.22146/agritech
Core Subject : Agriculture,
Agritech with registered number ISSN 0216-0455 (print) and ISSN 2527-3825 (online) is a scientific journal that publishes the results of research in the field of food and agricultural product technology, agricultural and bio-system engineering, and agroindustrial technology. This journal is published by Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta in colaboration with Indonesian Association of Food Technologies (PATPI).
Articles 15 Documents
Search results for , issue "Vol 36, No 3 (2016)" : 15 Documents clear
Evaluasi Perlakuan Pendahuluan Menggunakan Kalsium Hidroksida untuk Biokonversi Jerami Padi Menjadi L-Asam Laktat oleh Rhizopus oryzae AT3 Dhina Aprilia Nurani Widyahapsari; Retno Indrati; Sigit Setyabudi; Sardjono Sardjono
agriTECH Vol 36, No 3 (2016)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (622.937 KB) | DOI: 10.22146/agritech.16587

Abstract

L-lactic acid can be used as a precursor of polylactic acid (PLA). PLA is a biodegradable biomaterial commonly used for biodegradable plastics. Lactic acid can be produced from lignocelluloses materials such as rice straw. Rice straw is composed of cellulose and hemicellulose that can be hydrolyzed to fermentable sugar by cellulolytic and hemicellulolytic enzymes then converted to L-lactic acid by Rhizopus oryzae. As most cellulose and hemicellulose present in lignocellulose biomass are not readily accessible for these enzyme, pretreatment is required to alter the structure of lignocellulose substrates. This research aimed to investigate the effect of lime pretreatment on rice straw bioconversion to L-lactic acid by Rhizopus oryzae AT3. Rice straw was pretreated with lime (Ca(OH)2) at 85 °C for 16 hours. Unpretreated and pretreated rice straw were hydrolyzed using crude enzyme that produced by Trichoderma reesei Pk1J2. Enzyme production was carried out by solid state fermentation using rice straw and rice brand as substrate. Enzymatic hydrolysis was carried out in flasks. Each flask was added with unpretreated or pretreated rice straw, buffer citrate solution and crude enzyme then hydrolyzed for 0-96 hours. Hydrolysate was fermented by Rhizopus oryzae AT3 for 0-6 days by using adsorbed carrier solid-state fermentation method with polyurethane foam as inert support material. Lime pretreatment at 85 °C for 16 hour led to significant solubilisation of lignin and hemicellulose. It involved lignocellulose structure modified that enhance enzymatic hydrolysis and resulted higher reducing sugars than unpretreated rice straw. The high reducing sugars was not related to high lactic acid yields. Fermentation of pretreated rice straw hydrolysate by Rhizopus oryzae AT3 did not only produce L-lactic acid but also other compound. On the other hand, fermentation of unpretreated rice straw hydrolysate only produced L-lactic acid. ABSTRAKPolimerisasi asam laktat menjadi polylactic acid untuk menghasilkan biodegradable plastic membutuhkan asam laktat dengan isomer spesifik. Rhizopus oryzae adalah mikroorganisme yang spesifik menghasilkan L-asam laktat. Selain itu Rhizopus oryzae dapat menggunakan limbah pertanian seperti jerami padi sebagai substrat. Komponen utama jerami padi merupakan lignoselulosa yang dapat dihidrolisa secara enzimatis menjadi komponen gula sederhana penyusunnya dan selanjutnya dapat dikonversi menjadi L-asam laktat oleh Rhizopus oryzae. Namun struktur lignoselulosa sangat kompak dan rapat, sulit untuk dihidrolisa secara enzimatis sehingga diperlukan adanya perlakuan pendahuluan untuk merombak struktur lignoselulosa agar mudah dihidrolisa. Penelitian ini bertujuan untuk mengetahui pengaruh perlakuan pendahuluan menggunakan kalsium hidroksida (Ca(OH)2) terhadap biokonversi jerami padi menjadi L-asam laktat oleh Rhizopus oryzae AT3. Perlakuan pendahuluan pada jerami padi dilakukan menggunakan (Ca(OH)2) disertai pemanasan suhu 85 °C selama 16 jam. Jerami padi dengan dan tanpa perlakuan pendahuluan dihidrolisa secara enzimatis menggunakan crude enzyme yang diproduksi oleh Trichoderma reesei Pk1J2. Produksi crude enzyme dilakukan dengan fermentasi substrat padat dengan campuran jerami padi dan dedak sebagai substrat. Hidrolisat jerami padi dengan dan tanpa perlakuan pendahuluan selanjutnya difermentasi oleh Rhizopus oryzae AT3 menggunakan metode adsorbed carrier solid state fermentation dengan polyurethane foam (PUF) sebagai bahan pendukung. Perlakuan pendahuluan menggunakan Ca(OH2) disertai pemanasan suhu 85 °C selama 16 jam dapat merubah komposisi lignoselulosa jerami padi yaitu dengan melarutkan lignin dan hemiselulosa. Perubahan komposisi lignoselulosa memudahkan kerja crude enzyme dalam menghidrolisa jerami padi sehingga menghasilkan gula reduksi lebih tinggi dibandingkan jerami padi tanpa perlakuan pendahuluan. Tingginya gula reduksi tidak serta merta meningkatkan yield L-asam laktat yang dihasilkan. Fermentasi hidrolisat jerami padi dengan perlakuan pendahuluan oleh Rhizopus oryzae AT3 menghasilkan yield L-asam laktat lebih rendah dibandingkan hidrolisat jerami padi tanpa perlakuan pendahuluan. Namun pada jerami padi dengan perlakuan pendahuluan dihasilkan senyawa lain selain asam laktat.
Pengaruh Konsentrasi Alginat dan CaCl2 terhadap Kadar Antosianin, Aktivitas Antioksidan, dan Karakteristik Sensoris Buah Duwet (Syzygium cumini Linn) Restrukturisasi Lydia Ninan Lestario; Driana Herawati; Silvia Andini
agriTECH Vol 36, No 3 (2016)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (491.228 KB) | DOI: 10.22146/agritech.16588

Abstract

Restructuration is one of the ways for processing fresh fruit without heating, so the nutritional value of fruit can be maintained. This method has been applied to several types of tropical fruits but has never been applied to java plum fruit, while it has high antioxidant activity but has a short shelf life. The aim of this research was to produce the best formulation to make restructured java plum fruit. Fresh java plum fruit puree was mixed with several concentrations of calcium alginate (1 %, 2 %, 3 %, and 4 %) and CaCl2 (0.75 %; 1 %). Restructured fruit products were evaluated for total anthocyanin content, antioxidant activity, texture, pH, and sensory properties. The result of this research showed that 1 % alginate and 1 % CaCl2 gave restructured java plum fruit with the highest anthocyanin content 171.61 mg/100g (DW) and highest antioxidant activity 75.79 %. The overall organoleptic test with 20 panelists showed that restructured fruit with alginate 1 % and CaCl2 1 % or 0.75 % resulted the highest score 3.75. ABSTRAKRestrukturisasi merupakan salah satu cara pengolahan buah segar tanpa pemanasan sehingga nilai gizi buah dapat dipertahankan. Metode ini sudah diteliti pada beberapa jenis buah tropis tetapi belum pernah dilakukan terhadap buah duwet, padahal buah duwet memiliki aktivitas antioksidan tinggi tetapi memiliki daya simpan rendah. Penelitian ini bertujuan untuk menentukan formulasi terbaik pada pembuatan restrukturisasi buah duwet. Bubur buah duwet segar dicampur dengan berbagai konsentrasi alginat (1 %, 2 %, 3 %, dan 4 %) dan CaCl2 (0,75 %; 1 %). Produk buah hasil restrukturisasi diukur kandungan antosianin total, aktivitas antioksidan, tekstur, pH, dan sifat-sifat sensorisnya. Hasil penelitian menunjukkan bahwa alginat 1% dan CaCl2 1% memiliki kandungan antosianin tertinggi yaitu 171,61 mg/100g (BK) dan aktivitas antioksidan tertinggi yaitu 75,79 %. Uji organoleptik dengan 20 panelis menunjukkan bahwa produk restrukturisasi buah duwet yang paling disukai panelis diperoleh dari perlakuan alginat 1 % dan CaCl2 1 % atau 0,75 % dengan skor 3,75.
Pengaruh Perendaman Asam Askorbat dan Natrium Bisulfit pada Dua Varietas Bawang Merah (Allium ascalonicum L.) terhadap Sifat Fisikokimia dan Organoleptik Tepungnya Ermi Sukasih; Setyadjit Setyadjit
agriTECH Vol 36, No 3 (2016)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (547.373 KB) | DOI: 10.22146/agritech.16589

Abstract

Recently, there is an increase of shallot production in Indonesia. One alternative of flesh shallot processing is shallot powder making which can be used during the off season. The aim of the study was to observe the effect of immersion in ascorbic acid and sodium bisulphite on two varieties of shallot on physical and organoleptic characters of shallot powder. Drying was done using tray dryer. Experimental design used was factorial completely randomised design. The first factor was the type of immersion agent and duration time and the second factor was shallot varieties (Bima and Sembrani). Statistical mean test was using Tukey Honestly Significant (HSD). The result showed that the treatments significantly affected parameters such as moisture content, ascorbic acid, total phenol, anthocyanin, and antioxidant activity, free radical inhibition and color (chroma). From ranking test the best treatment was Bima variety with 0.2% ascorbic acid treatment for 30 min. Characteristic of the best treatment was with moisture content 4.03±0.79 % (w/w), ash content 4.45±0.13 % (w/w), fat content 1.24±0.64 % (w/w), protein content 15.56±0.28 % (w/w), 61.94±4.11 mg/100g, total phenolics 256.39±16.26 ppm, anthocyanins 50.70±2.31 ppm, quercetin 2612.40±408.94 ppm, antioxidant activity ekuivalent, 225.72±4.0 µg/mL, inhibition free radical 83.05±1.69 %, 85.02 hue value (yellowish red) and aroma score 3.2 (strong), brightness score 4.2 (bright) and color 1.25 (pale red). ABSTRAKKenaikan cukup signifikan pada produksi bawang merah terjadi di Indonesia. Salah satu alternatif pengolahan bawang merah segar adalah melalui pengolahan menjadi tepung sehingga tetap tersedia saat langka dan harganya tinggi. Penelitian ini bertujuan untuk melihat pengaruh perendaman asam askorbat dan natrium bisulfit pada dua varietas bawang merah terhadap sifat fisikokimia dan organoleptik tepung yang dihasilkan. Pengeringan dilakukan dengan tray dryer. Percobaan menggunakan rancangan acak lengkap faktorial, faktor pertama jenis bahan  dan lama perendaman dan faktor kedua adalah varietas bawang merah (Bima dan Sembrani). Analisis statistik menggunakan metode Tukey Honestly Significant Data (HSD). Hasil penelitian menunjukkan bahwa perlakuan berpengaruh nyata terhadap parameter kadar air, vitamin C, total fenolik, antosianin, aktivitas antioksidan, inhibisi radikal bebas dan warna (chroma). Berdasarkan uji peringkat diperoleh perlakuan terbaik adalah tepung bawang merah dari varietas Bima dengan perlakuan perendaman asam askorbat 0,2 % selama 30 menit. Karakteristik dari tepung bawang ini memiliki  kadar air 4,03 ± 0,79 % (bb), kadar abu 4,45 ± 0,13 % (bb), lemak 1,24 ± 0,64 % (bb) protein 15,56 ± 0,28 % (bb), vitamin C 61,94 ± 4,11 mg/100 g, total fenol 256,39 ± 16,26 ppm, antosianin 50,70 ± 2,31 ppm, kuersetin 2612,40 ± 408,94 ppm, aktivitas antioksidan ekuivalen 225,72 ± 4,07 µg/mL, inhibisi radikal bebas 83,05 ± 1,69 %, nilai oHue 85,02 (kuning kemerahan) dan skor aroma 3,2 (kuat), skor kecerahan 4,2 (terang) dan skor warna 1,25 (merah pucat).   
Pengaruh Pemberian Margarin terhadap Sifat Fisiko Kimiawi dan Sensoris Sosis Ayam Petelur Afkir Hendronoto Arnoldus Walewengko Lengkey; Sofi Margritje Sembor; Dani Garnida; Primiani Edianingsih; Nanah Nanah; Roostita Lobo Balia
agriTECH Vol 36, No 3 (2016)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (442.602 KB) | DOI: 10.22146/agritech.16590

Abstract

Research was aimed to determine the effect of margarine application on the physicochemical and sensory properties of culled layer hens sausages. In addition to utilizing the culled layer hens meat as a source of animal protein, as well as to diversify food, so that the resulting product can be accepted by consumers. This study was conducted in a completely randomized experimental design with four treatments unidirectional pattern with margarine giving addition 0.0 %; 2.5 %; 5.0 % and 7.5 % with four replications. The data obtained were statistically tested by analysis of variance (ANOVA), if there is a noticeable difference, then it continued to Duncan's multiple range test. The analysis showed that the water content, fat content, and pH sausage has significant effect, but the protein content was not significantly between the sausages were not given margarine (P-1) and the sausages are given margarine (P-2, P-3 and P-4). The sausage produced will increase the protein content, fat content, and pH with increasing percentage of margarine given, otherwise the moisture content will decrease. Based on sensory testing (appearance, color, flavor, texture and total acceptance), culled layer hens sausage can be accepted by consumers. Sausages were given margarine value ranges between 7.0 to 8.4 (just like - really like) and were not given margarine has lower value ranging from 5.2 to 6.9 (neutral - just like). ABSTRAKPenelitian mengenai pengaruh pemberian margarin terhadap sifat fisiko kimiawi dan sensoris sosis ayam petelur afkir adalah untuk mengetahui penambahan margarin terhadap kualitas sosis ayam petelur afkir, selain itu untuk memanfaatkan daging ayam petelur afkir sebagai sumber protein hewani, dan untuk melakukan penganekaragaman pangan, sehingga produk yang dihasilkan dapat diterima oleh konsumen. Penelitian ini dilakukan secara eksperimental dengan rancangan acak lengkap pola searah dengan empat perlakuan pemberian margarin 0,0 %; 2,5 %; 5,0 % dan 7,5 % dengan empat kali ulangan. Data yang diperoleh diuji secara statistik dengan analisa varian (ANOVA), apabila terdapat perbedaan yang nyata dilakukan Uji jarak berganda Duncan. Hasil analisis menunjukkan bahwa kadar air dan kadar lemak dan pH sosis berpengaruh nyata namun kadar protein tidak berpengaruh nyata antara sosis yang tidak diberikan margarin (P-1) dan sosis yang diberikan margarin (P-2, P-3, dan P-4). Sosis yang dihasilkan akan semakin meningkat kadar protein, kadar lemak dan pH dengan bertambahnya persentasi margarin yang diberikan, sebaliknya kadar airnya akan menurun. Berdasarkan uji sensoris (tampilan, warna, flavor, tekstur dan total penerimaan), maka sosis ayam petelur afkir dapat diterima oleh konsumen. Sosis yang diberi margarin nilainya berkisar antara 7,0 – 8,4 (cukup suka – sangat suka) dan yang tidak diberi margarin nilainya lebih rendah yaitu berkisar antara 5,2 – 6,9 (netral – cukup suka).
Penggunaan Ekstrak Kasar Polisakarida Larut Air dari Biji Buah Durian (Durio zibethinus Murr.) pada Pembuatan Saos Tomat Herlina Herlina; Triana Lindriati; Prahasbynar Prahasbynar
agriTECH Vol 36, No 3 (2016)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (409.182 KB) | DOI: 10.22146/agritech.16597

Abstract

Tomato sauce is one of the diversification of tomato products that could increase the added value and shelf life of tomatoes. Sauce made from tomato has a high water content (94 %) resulting a watery sauce product. The purpose of this study was to determine the effect of crude extract Water Soluble Polysaccharide (WSP) from durian seeds on physical, chemical, and organoleptic as characteristics oftomato sauce. The use of crude extract WSP from durian seeds is expected to increase the viscosity of the sauce. The experimental design used in this study was a non factorial randomized complete block design with three replications. Parameters measured were viscosity, color (Hue and Chroma), total dissolved solids, pH, vitamin C, and organoleptic (panelist preferences) in addition to the effectiveness test to determine the best treatment. The results show that the tomato sauce composition significantly affect the viscosity, pH, color intensity, and total dissolved solids, while no significant effect on the angle of color and vitamin C of tomato sauce. The effectiveness test showed that the panelists preferred the P4 tomato sauce (use crude extract WSP from seeds fruit of durian 0.8 %) with fondness value  color of 2.68; aroma of 3.08; flavor of 3.48; viscosity of 3.44; overall liking of 3.56. The value of viscosity, hue, chroma, total dissolved solids, pH, vitamin C were 39.64 centipoise; 38,34°; 20.16; 35° Brix; 4.92; and 4.95 mg/g, respectively. ABSTRAKSaos tomat merupakan salah satu diversifikasi produk olahan buah tomat yang dapat meningkatkan nilai tambah dan umur simpan buah tomat. Saos yang berbahan dasar tomat, memiliki kandungan air yang tinggi (94 %) sehingga menghasilkan saos yang encer. Tujuan penelitian ini adalah untuk mengetahui pengaruh penggunaan ekstrak kasar Polisakarida Larut Air (PLA) dari biji buah durian terhadap sifat fisiko kimia dan organoleptik saos tomat. Penggunaan ekstrak kasar PLA dari biji buah durian diharapkan dapat meningkatkan viskositas saos tomat. Rancangan penelitian yang digunakan adalah rancangan acak kelompok faktor tunggal dengan tiga kali ulangan. Parameter yang diamati adalah viskositas, warna (Hue dan Chroma), total padatan terlarut, pH, kadar vitamin C, dan uji organoleptik (kesukaan panelis) yang dilanjut dengan uji efektivitas untuk menentukan perlakuan terbaik. Hasil penelitian menunjukkan bahwa komposisi saos tomat berpengaruh nyata terhadap viskositas, pH, intensitas warna, dan total padatan terlarut, tetapi tidak berpengaruh nyata terhadap sudut warna dan kadar vitamin C saos tomat. Uji efektivitas menunjukkan bahwa saos tomat yang disukai panelis adalah perlakuan P4 (penggunaan ekstrak kasar PLA dari biji buah durian sebesar 0,8 %) dengan nilai kesukaan warna sebesar 2,68; kesukaan aroma sebesar 3,08; kesukaan rasa sebesar 3,48; kesukaan kekentalan sebesar 3,44; kesukaan keseluruhan sebesar 3,56, dan nilai viskositas, hue, chroma, total padatan terlarut, pH, vitamin C berturut-turut adalah 39,64 centipoise; 38,34°; 20,16; 35° Brix; 4,92; dan 4,95 mg/g.
Pendugaan Kandungan Kimia Mangga Gedong Gincu Menggunakan Spektroskopi Inframerah Dekat Herna Permata Sari; Yohanes Aris Purwanto; I Wayan Budiastra
agriTECH Vol 36, No 3 (2016)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (567.642 KB) | DOI: 10.22146/agritech.16599

Abstract

The objective of  this work was to predict the soluble solid content, total acid, sugar acid ratio, and crude fiber of ‘Gedong Gincu’ mango non destructive using Near infrared Spectroscopy. Experiments were carried out using 182 samples of ‘Gedong Gincu’ mango. NIR reflectance spectra measurements were performed at wavelength of 1000-2500 nm using NIRFlex N-500 fiber optic solid. References data were collected from laboratory measurements. Five pre-processing treatments, smoothing 3 points (sa3), normalization (n01), first derivative Savitzky-Golay 9 points (dg1), combination (n01, dg1), and the Multiplicative Scatter Correction (MSC) were used to improve the accuracy of the calibration model. Partial Least Square (PLS) method was used to calibrate NIR data through references data. The results show  that the best method for prediction of soluble non solid spectra were MSC and 12 factor of PLS with calibration value of Correlation Coefficient (r), Square Error Calibration (SEC), Square Error Prediction (SEP),  Ratio of standard error prediction to deviation (RPD) were 0.91, 0.25 %, 0.39 %, 2.14 respectively. Sugar acid ratio content was predictd using  MSC and 12 factor of PLS calibration with values of r, SEC, SEP, RPD were 0.81, 32.08 °Brix/%, 38.44 °Brix/%, 1.45. Soluble solid content was predicted using sa3 and 15 factor of PLS calibration with values of  r, SEC, SEP, RPD were 0.82, 1.04 °Brix, 1.28 °Brix, 1.52 respectively. Total acid was predicted using  dg1 and 3 with the value of  r, SEC, SEP, RPD were 0.74, 0.01 %, 0.12 %, 1.33 respectively. It could be concluded  that the developed model could be used to predict the chemical contents of ‘Gedong Gincu’ mango non destructively. ABSTRAKTujuan dari penelitian ini adalah memprediksi kandungan total padatan terlarut (TPT), total asam, rasio gula asam, dan padatan tidak terlarut (serat kasar) mangga Gedong Gincu secara non destruktif menggunakan spektroskopi inframerah dekat (NIR). Bahan yang digunakan berupa mangga Gedong Gincu sebanyak 182 buah. Pengukuran spektra reflektan NIR dilakukan pada panjang gelombang 1000 – 2500 nm menggunakan NIRFlex N-500 fiber optik solid dilanjutkan pengukuran data referensi laboratorium. Lima pra-proses data spektra yaitu smoothing 3 points (sa3), normalisasi (n01), first derivative Savitzzky-golay (dg1), kombinasi (n01,dg1), dan Multiplicative Scatter Correction (MSC) dilakukan untuk meningkatkan akurasi model kalibrasi. Kalibrasi data NIR dan data kimia dilakukan menggunakan metode Partial Least Square (PLS). Metode terbaik untuk prediksi padatan tidak terlarut diperoleh dengan pra-proses MSC dan kalibrasi PLS dengan nilai Correlation Coefficient (r), Square Error Calibration (SEC), Square Error Prediction (SEP), Ratio of standard error prediction to deviation (RPD) adalah 0,91, 0,25 %, 0,39 %, 2,14, dan faktor PLS 12. Kandungan rasio gula asam diduga dengan pra-proses MSC serta kalibrasi PLS dengan nilai r, SEC, SEP, RPD adalah 0,81, 32,08 °Brix/%, 38,44 °Brix/%, 1,45 dan faktor PLS yang digunakan 12. TPT diduga menggunakan pra-proses sa3 dan kalibrasi PLS dengan nilai r, SEC, SEP, RPD adalah 0,82, 1,04 oBrix, 1,28 °Brix, 1,52. Model kalibrasi total asam diperoleh pra-proses dg1 dan kalibrasi PLS dengan nilai r, SEC, SEP, RPD adalah 0,74, 0,01 %, 0,12 %, 1,33. Hasil penelitian ini menunjukkan bahwa model yang dikembangkan dapat digunakan untuk menduga kandungan kimia mangga Gedong Gincu secara non destruktif.
Optimisasi Suhu Pemanasan dan Kadar Air pada Produksi Pati Talas Kimpul Termodifikasi dengan Teknik Heat Moisture Treatment (HMT) I Nengah Kencana Putra; Ni Wayan Wisaniyasa; Anak Agung Istri Sri Wiadnyani
agriTECH Vol 36, No 3 (2016)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (585.872 KB) | DOI: 10.22146/agritech.16602

Abstract

One of the physically starch modification technique is heat-moisture treatment (HMT). This technique can increase the resistance of starch to heat, mechanical treatment, and acid during processing.  This research aimed to find out the influence of heating temperature and moisture content in the modification process of cocoyam starch  with HMT techniques on the characteristic of product, and then to determine the optimum heating temperature and moisture content in the process. The research was designed with a complete randomized design (CRD) with two factors factorial experiment.  The first factor was temperature of the heating consists of 3 levels namely 100 °C, 110 °C, and 120 °C. The second factor was the moisture content of starch which consists of 4 levels, namely 15 %, 20 %, 25 %, and 30 %. The results showed that the heating temperature and moisture content significantly affected water content, amylose content and swelling power of modified cocoyam starch product, but the treatment had no significant effect on the solubility of the product. HMT process was able to change the type of cocoyam starch from type B to type C. The optimum heating temperature and water content on modified cocoyam starch production process was 110 °C and 30 % respectively. Such treatment resulted in a modified cocoyam starch with moisture content of 6.50 %, 50,14 % amylose content, swelling power of 7.90, 0.0009% solubility, paste clarity of 96.310 % T, and was classified as a type C starch. ABSTRAKSalah satu teknik modifikasi pati secara fisik adalah teknik Heat Moisture Treatment (HMT). Teknik ini dapat meningkatkan ketahanan pati terhadap panas, perlakuan mekanik, dan asam selama pengolahan. Penelitian ini bertujuan untuk mengetahui pengaruh suhu dan kadar air pada proses modifikasi pati talas kimpul dengan teknik HMT terhadap karakteristik produk, dan selanjutnya menentukan suhu dan kadar air yang optimal dalam proses tersebut. Penelitian ini dirancang dengan rancangan acak lengkap (RAL) dengan percobaan faktorial dua faktor. Faktor pertama adalah suhu pemanasan, terdiri dari 3 taraf yaitu 100 °C, 110 °C, dan 120 °C. Faktor kedua adalah kadar air pati, terdiri dari 4 taraf yaitu 15 %, 20 %, 25 %, dan 30 %. Hasil penelitian menunjukkan, suhu pemanasan dan kadar air pati secara nyata mempengaruhi kadar air, kadar amilosa dan swelling power pati talas kimpul termodifikasi, tetapi tidak berpengaruh nyata terhadap kelarutannya. Proses HMT mampu mengubah tipe pati talas kimpul dari tipe B menjadi tipe C. Suhu pemanasan dan kadar air optimal pada produksi pati talas kimpul termodifikasi adalah 110 °C dan 30 %. Perlakuan tersebut menghasilkan pati talas kimpul termodifikasi dengan karakteristik seperti kadar air 6,50 %, kadar amilosa 50,14 %, swelling power 7,90, kelarutan 0,001 %, kejernihan pasta 96,31 % T, dan dengan klasifikasikan pati tipe C.
Evaluation of Major Fatty Acids Determination in Palm Oil by Gas Chromatography-Flame Ionization Detection Moh Taufik; Hanifah Nuryani Lioe; Nancy Dewi Yuliana
agriTECH Vol 36, No 3 (2016)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (549.026 KB) | DOI: 10.22146/agritech.16603

Abstract

The fatty acid composition of palm oil is the major factor influencing its physical and chemical properties. The purpose of this research was to evaluate the analytical performance of major fatty acids (palmitic acid, stearic acid, oleic acid, and linoleic acid) analysis in palm oil. Triglycerides of palm oil were derivatized to fatty acid methyl esters (FAMEs) by using boron trifluoride (BF3) in methanol. FAMEs were determined by gas chromatography-flame ionization detection (GC-FID) using DB-23 capillary column as stationary phase. The studied parameters were instrument performance analysis, the efficiency of fatty acid derivatization, stability of derivatized analytes, accuracy, repeatability, intra-lab reproducibility, ruggedness, and method uncertainty. The evaluation results showed the instrument linearity at a working range of 5 to 40 mg/mL marked by coefficient of determination (R2) between 0.991-0.995. Instrument limits of detection (LOD) and instrument limits of quantification (LOQ) for 4 major fatty acids analysis were 26-35 µg/mLand 86-128 µg/mL, respectively. The increase of fatty acid concentration led to the decrease of derivatization efficiency in the fatty acids analysis. The result also showed that derivatized analytes were stable during 24 h storage at freeze temperature. The average recovery values by spiking method with the spiking concentration at 50 and 90 mg/g sample were at 75-94 % for stearic and linoleic acids analysis, however those for palmitic and oleic acids analysis were considered very low (<40 %), due to their low derivatization efficiency. Repeatability and intra-lab reproducibility of 4 major fatty acids analysis were at acceptable ranges, 0.45-1.38 % and 1.15-2.03 %, respectively. Determination by varying the volume of derivatizing agent showed the rugged method. Uncertainty of repeatability (Ur) and uncertainty of reproducibility (Ur) were ranged at 1.84-9.02 mg/g and 1.40-10.65 mg/g, respectively. This method was considerably reliable for the analysis of less abundance fatty acids in palm oil, stearic and linoleic acids.
Identifikasi Bakteri Asam Laktat dan Aktivitas Penghambatan Radikal pada Jaruk Tigarun (Crataeva nurvala, Buch Ham) Narzani Rahmi; Eni Harmayani; Umar Santosa; Purnama Darmadji
agriTECH Vol 36, No 3 (2016)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (537.862 KB) | DOI: 10.22146/agritech.16604

Abstract

Jaruk tigarun is a traditional fermented food from South Borneo made from tigarun flower that was fermented in water without salt addition. The objectives of the research were to isolate and identify the Lactic Acid Bacteria (LAB) from jaruk tigarun then to determine the DPPH radical scavenging activities of jaruk tigarun extracts. LAB was isolated from jaruk tigarun which was fermented for 7 days at room temperature. Media of MRS+CaCO3 were used to isolate the LAB while API 50 CHL kit was utilised to identify them. Fresh flowers and jaruk tigarun were also freeze-dryed, crushed and extracted using methanol, ethanol and ethyl acetate. The total phenolic and antioxidant activity of each extract were determine with Folin-Ciocalteu method and DPPH. Three isolates of LAB were isolated and identified as Lactobacillus plantarum. The fermentation was able to increase total phenolic of jaruk tigarun which was extracted with methanol, ethanol, and ethyl acetate (53.24 ± 0.73, 44.86 ± 0.90 and 23.95 ± 0.13 mg GAE/g extract, respectively). Similarly, the antioxidant activity of jaruk tigarun that were extracted with methanol, ethanol and ethyl acetate increased to 92.68 ± 0.02 %, 92.43 ± 0.11 %, and 42.94 ± 0.02 %. Methanolic extract of jaruk tigarun has the highest IC50 that was equal to 1.511 μg/mL. UV-Vis spectrum analysis and FT-IR were used to identify the compounds isolated from methanolic extract of jaruk tigarun resulting flavonoid as tentative identified compounds. ABSTRAKJaruk tigarun adalah salah satu makanan fermentasi tradisional dari Kalimantan Selatan yang dibuat dengan cara merendam bunga tigarun dalam air matang selama beberapa hari. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri asam laktat yang terlibat selama fermentasi jaruk serta mengetahui aktivitas penghambatan radikal DPPH pada jaruk tigarun. BAL diisolasi dari bunga tigarun yang difermentasi selama 7 hari pada suhu kamar sampai menjadi jaruk. Isolasi BAL menggunakan media MRS+CaCO3 dan diidentifikasi dengan API 50 CHL Kit. Bunga segar dan jaruk tigarun juga masing-masing dikeringbekukan, dihaluskan dan diekstraksi dengan metanol, etanol dan etil asetat. Total fenolik dan aktivitas antioksidan dari masing masing ekstrak ditentukan dengan metoda Folin Ciocalteu dan DPPH. Tiga isolat BAL berhasil diisolasi dan diidentifikasi sebagai Lactobacillus plantarum. Fermentasi berhasil meningkatkan total fenolik dari jaruk tigarun yang diekstrak dengan metanol, etanol dan etil asetat berturut-turut sebesar 53,24 ± 0,73, 44,86 ± 0,90 dan 23,95 ± 0,13 mg GAE/g ekstrak. Demikian pula halnya dengan aktivitas antioksidan dari jaruk yang diekstrak dengan metanol, etanol dan etil asetat  meningkat menjadi 92,68 ± 0,02 %, 92,43 ± 0,11 %, dan 42,94 ± 0,02 %. Ekstrak metanol dari jaruk tigarun  memiliki IC50  yang paling baik yaitu sebesar 1.511 μg/mL. Hasil isolasi dan identifikasi senyawa yang dimurnikan dari ekstrak metanol jaruk menggunakan analisis spektrum UV-Vis dan FT-IR diperoleh dugaan senyawa adalah golongan flavonoid.
Ekstraksi Pektin dari Kulit dan Tandan Pisang dengan Variasi Suhu dan Metode Nurhayati Nurhayati; Maryanto Maryanto; Rika Tafrikhah
agriTECH Vol 36, No 3 (2016)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (508.13 KB) | DOI: 10.22146/agritech.16605

Abstract

The increase in banana production will lead to the number of wastes, such as peels and bunches.  In spite of having a potential usage for food industries, banana waste has not been optimally utilized yet. It contains a high value of natural substance, i.e. pectin, which is composed of pectin galacturonic acid molecules to form poligalacturonic acid. Pectin is used as a stabilizer in fruit juice, jelly, jam and marmalade. The objectives of this research were to determine the characteristics of the flour of banana peels and bunches and to evaluate the effect of temperature treatments (60 °C and 80 °C) and extraction methods (one, two and three cycles) on the yield of pectin and the characteristics of the extracted pectin. The extractions of pectin were conducted by water at ratio flour to water 1:54 (one cycle of extraction), 1:27 (two cycles of extraction) and 1:18 (three cycles of extraction). The result showed that the banana waste flour contain the water content ranged from 8.14 to 9.05 %, the highest level of pectin was found in banana peels var. embug (4.54 %) and the value of the whiteness degree ranged from 50.80 to 55.21 %. The yield of the extracted pectin can be extracted optimum at temperature 80 °C with two-cycle extraction. Banana peels contain more pectin than the banana bunches. The value of whiteness degree of the extracted pectin was ranged from 31.31 to 38.12 %. The functional groups of the pectin composed of alcohol groups (primary, secondary and tertiary), primary amines, amides (monosub and disub) and carbonate covalent. ABSTRAKSeiring peningkatan produksi pisang tentu akan diikuti dengan peningkatan limbah pisang seperti kulit dan tandan buah pisang. Pemanfaatan limbah pisang tersebut masih belum optimal. Padahal di dalamnya terkandung substansi alami tanaman yang memiliki nilai guna tinggi yaitu pektin yang tersusun atas molekul asam galakturonat membentuk asam poligalakturonat. Pektin dimanfaatkan sebagai bahan penstabil pada sari buah, jelly, jam dan marmalade. Penelitian ini bertujuan untuk mengetahui karakteristik tepung dari kulit dan tandan pisang, mengetahui pengaruh perlakuan suhu (60 °C dan 80 °C) dan metode ekstraksi (satu, dua dan tiga tingkat) terhadap rendemen pektin, serta karakteristik pektin yang terekstrak. Limbah pisang berasal dari pisang varietas agung dan embug. Ekstraksi pektin dilakukan dengan menggunakan pelarut air pada perbandingan tepung dan air sebesar 1:54 (ekstraksi satu tingkat), 1:27 (ekstraksi dua tingkat) dan 1:18 (ekstraksi tiga tingkat). Hasil penelitian menunjukkan karakrestik tepung limbah pisang yaitu kadar air berkisar antara 8,14 sampai dengan 9,05 % dengan kadar pektin tertinggi terdapat pada kulit pisang embug 4,54 % dan derajat putih tepung limbah pisang berkisar antara 50,80 sampai dengan 55,21 %. Rendemen pektin dapat terekstrak optimal pada kondisi ekstraksi suhu ekstraksi 80 °C dengan dua tingkat ekstraksi. Kulit pisang mengandung pektin lebih banyak daripada tandan pisang. Pektin yang terekstrak memiliki derajat putih sekitar 31,31 sampai dengan 38,12 %. Gugus fungsi pektin limbah pisang tersusun atas gugus alkohol (primer, sekunder dan tersier), amina primer, amida (monosub dan dwisubtitusi) serta karbonat kovalen.

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