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Global Medical and Health Communication
Published by Universitas Islam Bandung
ISSN : 23019123     EISSN : 24605441     DOI : https://doi.org/10.29313/gmhc
Core Subject : Health, Science,
Dentistry Health Professions Immunology & microbiology Nursing Public Health
Global Medical and Health Communication is a journal that publishes research articles on medical and health published every 4 (four) months (April, August, and December). Articles are original research that needs to be disseminated and written in English. Subjects suitable for publication include but are not limited to the following fields of anesthesiology and intensive care, biochemistry, biomolecular, cardiovascular, child health, dentistry, dermatology and venerology, endocrinology, environmental health, epidemiology, geriatric, hematology, histology, histopathology, immunology, internal medicine, nursing sciences, midwifery, nutrition, nutrition and metabolism, obstetrics and gynecology, occupational health, oncology, ophthalmology, oral biology, orthopedics and traumatology, otorhinolaryngology, pharmacology, pharmacy, preventive medicine, public health, pulmonology, radiology, and reproductive health.
Arjuna Subject : Kedokteran - Epidemilogi
Articles 12 Documents
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Search results for , issue "Vol 7, No 1 (2019)" : 12 Documents clear
Effectiveness of Various Mosquito Attractant Solutions to Control Mosquito Population Faridah, Lia; Albert, Christian; Fauziah, Nisa
Global Medical & Health Communication (GMHC) Vol 7, No 1 (2019)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (777.125 KB) | DOI: 10.29313/gmhc.v7i1.2974

Abstract

The vector-borne disease is a disease caused by an organism that can transmit disease between human or animal to human. In Indonesia, several vector-borne diseases are a burden of the government including dengue fever, chikungunya, filariasis, and malaria. The attractive baited lethal ovitrap (ALOT) is a novel strategy to alleviate mosquito populations in three main actions: attraction, an adulticide, and larvacide. Research using plant infusion can attract mosquitoes to lay their eggs is needed. This study aims to compare the effectiveness of the mosquito repellent solution using materials from organic waste in Bandung. This study was a quantitative analytic study with a quasi-experimental design conducted in the Faculty of Medicine Universitas Padjadjaran area in October 2016–July 2017. Research subjects are mosquito eggs in a solution which placed at 25 different places for every solution. The analysis was performed using Kruskal-Wallis test followed by the Dunn test. The result of the Kruskal-Wallis test indicates the difference of effectiveness of each solution (p<0.05). Based on the results of the Dunn test, the most significant difference found in the solution of wood shavings with the vegetable waste solution and the solution of wood shavings with corn straw (p<0.05). In conclusion, there is a difference in the effectiveness of the mosquito repellent solutions and the most attractive solution for mosquitoes to oviposit is the corn straw solution. EFEKTIVITAS BERBAGAI LARUTAN PENARIK NYAMUK UNTUK MENGONTROL POPULASI NYAMUKPenyakit tular vektor adalah penyakit yang disebabkan oleh organisme yang dapat mentransmisikan penyakit antarmanusia atau hewan ke manusia. Di Indonesia, terdapat beberapa penyakit tular vektor yang masih menjadi beban pemerintah, di antaranya demam berdarah, chikungunya, penyakit kaki gajah, dan malaria. Attractive baited lethal ovitrap (ALOT) merupakan strategi baru untuk menurunkan populasi nyamuk dalam tiga aksi utama, yaitu attraction, adulticide, dan larvacide. Penelitian terkait larutan dari tanaman yang dapat menarik nyamuk sangat diperlukan. Penelitian ini bertujuan membandingkan efektivitas larutan penarik nyamuk dengan menggunakan bahan dari limbah organik yang ada di Kota Bandung. Penelitian ini adalah penelitian analitik kuantitatif dengan desain quasi-eksperimental yang dilakukan di lingkungan Fakultas Kedokteran Universitas Padjadjaran pada bulan Oktober 2016–Juli 2017. Subjek penelitian merupakan telur nyamuk yang ada pada larutan yang diletakkan pada 25 titik untuk setiap larutan. Analisis dengan Uji Kruskal-Wallis yang dilanjutkan dengan Uji Dunn. Hasil penelitian dengan Uji Kruskal-Wallis menunjukkan perbedaan efektivitas tiap-tiap larutan (p<0,05). Berdasar atas hasil Uji Dunn, perbedaan yang paling signifikan terdapat pada larutan serutan kayu dengan larutan sampah sayur dan larutan serutan kayu dengan jerami jagung (p<0,05). Simpulan, terdapat perbedaan efektivitas larutan penarik nyamuk dan larutan yang menarik nyamuk paling banyak untuk bertelur adalah larutan jerami jagung.
Primary Hippocampal Cell Culture and Its Application in Medical Researches Atik, Nur; Nandika, Alfya; Avriyanti, Erda; Bhatara, Tryando; Kartiwa, Raden Angga
Global Medical & Health Communication (GMHC) Vol 7, No 1 (2019)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (299.69 KB) | DOI: 10.29313/gmhc.v7i1.4245

Abstract

Studies in neuroscience can be performed in vitro and in vivo. In vivo studies will show significant results, but it is difficult to do and time-consuming. Primary hippocampal cell culture widely has used in neurobiological studies such as identifying the cellular mechanism of proteins, neuronal activity, and characteristics. The results of studies conducted on this cell culture will be very useful in discovering pathogenesis of a disease, the effect of a substance on the neuron, and neural basis of memory and learning. However, currently in Indonesia, primary hippocampal cell culture is still rare and difficult to do. The purpose of this study was to demonstrate that primary hippocampal cell culture can be done and developed in Indonesia and to review the application of it in medical researches. The study was an experimental study by obtaining neurons from animal’s hippocampus was conducted in 2015–2018 at Department of Cell Biology, Graduate School of Medicine Osaka University and Faculty of Medicine Universitas Padjadjaran. The experimental animal was mice embryo gathered 17.5-days postcoitus. Enzymatic and mechanical methods collected primary hippocampal cells. The cells counted and cultured, which later were observed to see neuron differentiation. The average number of culture cells from 3 embryonic’s hippocampus were 2.39×106. Neuron differentiation observed on the first day and more visible and numerous on the third day after plating. In conclusion, primary hippocampal cell culture using hippocampus from one hemisphere of embryonic mice brain showed a sufficient number of cells to carry out research and showed neuron differentiation. KULTUR SEL PRIMER HIPOKAMPUS DAN PENGGUNAANNYA DALAM RISET KEDOKTERANPenelitian dalam neurobiologi dapat dilakukan secara in vitro dan in vivo. Penelitian secara in vivo sangat berdampak hasilnya, namun sulit dan memakan waktu yang lama. Kultur sel primer hipokampus banyak digunakan dalam penelitian neurobiologi seperti melihat mekanisme protein seluler, serta aktivitas dan karakteristik neuron. Hasil penelitian yang dilakukan pada kultur sel ini akan sangat bermanfaat dalam menemukan proses suatu penyakit, efek suatu zat terhadap sel saraf, dan kemampuan belajar serta memori. Akan tetapi, saat ini di Indonesia kultur sel primer hipokampus masih jarang dan sulit dilakukan. Tujuan penelitian ini adalah menunjukkan bahwa kultur sel hipokampus primer dapat dilakukan dan dikembangkan di Indonesia, serta meninjau penerapannya dalam riset kedokteran. Penelitian ini merupakan studi eksperimental dengan mengoleksi neuron dari hipokampus hewan coba yang dilakukan pada tahun 2015–2018 di Department of Cell Biology, Graduate School of Medicine Osaka University dan Fakultas Kedokteran Universitas Padjadjaran. Hewan coba berupa embrio mencit hari ke-17,5 pascakoitus. Sel primer hipokampus dikoleksi untuk dihitung dan dikultur menggunakan metode enzimatik dan mekanik. Observasi neuron pada kultur dilanjutkan dengan mengamati diferensiasi neuron. Rerata jumlah sel kultur dari 3 hipokampus adalah 2,39×106. Diferensiasi neuron sudah tampak pada hari pertama dan makin jelas serta tampak pada hari ketiga pascapenanaman. Simpulan, kultur sel primer hipokampus menggunakan hipokampus dari salah satu sisi hemisfer otak menunjukkan jumlah sel yang cukup untuk melakukan suatu penelitian dan menunjukkan diferensiasi dari neuron.

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