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Journal of Tropical Biodiversity and Biotechnology

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Published by Universitas Gadjah Mada

ISSN : 25409573 EISSN : 25409581 DOI : -

Core Subject : Science, Agriculture, Social,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

Journal of Tropical Biodiversity and Biotechnology (JTBB) is an authoritative source of information concerned with the advancement of tropical biology studies in the Southeast Asia Region. It publishes original scientific work related to a diverse range of fields in tropical biodiversity, functional biology, and biotechnology.

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Articles 5 Documents

Search results for , issue "Vol 3, No 1 (2018): April" : 5 Documents clear

Floristic and Soil Characteristic at The RPH Sentul-Probolinggo Protected Low Land Forest, East Java Abban Putri Fiqa; Agung Sri Darmayanti
Journal of Tropical Biodiversity and Biotechnology Vol 3, No 1 (2018): April
Publisher : Universitas Gadjah Mada

East Java’s forest has a less ideal area based on predetermined requirements. Therefore, rehabilitation was done to meet these requirements and to improve the quality of existing forests. This research was conducted to identify the character of protected forest in Resort Pemangku Hutan (RPH) Sentul area, about vegetation structure and abiotic factor of lowland forest area with low rainfall. The research was conducted by explorative method with plot making for tree plant, sapling and ground cover to inventory and determine the forest vegetation structure inside the area, also by measuring the environmental parameters (temperature, humidity and light intensity) and making soil sampling to analyze biophysical condition. Data were analyzed descriptively both on vegetation condition and physical condition of land and its environment. The result showed that there were identified 84 species from 39 plant families either as trees, sapling or ground cover, with species of Moraceae as the largest contributor. The diversity index of the three types of vegetation shows a medium categorized, while the index of evenness indicates a good categorized that is close to one. Based on the existence of exotic species, especially on ground cover, within protected forest areas, this area has been degraded although not large. However, the physical condition of the soil and environmental conditions in the forest still indicate that this area is still quite good when used as a reference area on rehabilitation program with similar geographical conditions.Keywords: vegetation structure, soil characteristic, low land forest, RPH Sentul Probolinggo

Keragaman Genus Anadara Berdasarkan Karakter Morfologis dan Habitat di Perairan Pantai, Kota Tarakan, Kalimantan Utara Z. Zainuddin; Nyoman Puniawati Soesilo; T. Trijoko
Journal of Tropical Biodiversity and Biotechnology Vol 3, No 1 (2018): April
Publisher : Universitas Gadjah Mada

Bivalves are actively captured to be used as foodstuffs and raw materials souvenirs. Bivalves have economic value that continues to be exploited. But there is no record of its biodiversity in the coastal waters Tarakan City. This research purposes were to study the members of the Genus Anadara species diversity, study of morphological characters and its habitats in the waters of Tarakan. The method used in sampling was transect methods. Four stations have been set i.e. Binalatung, Selayung, Juata and airport. The obtained bivalves were identified based on its morphological characters. Physicochemical of the water, substrate, and the substrate texture in each station were measured. The morphological data was analyzed using descriptive analysis, while the relationships between shells’ morphometric was analyzed using linear regression analysis.The result of this research showed that there are two species, Anadara granosa and Anadara nodifera. Morphological character showed that there are radial ribs both of species. Habitat character is dominated by sand and mud. Morphometric relationships shells can be used to distinguish each species.

The Production of Corn Kernel Miso Based on Rice-koji Fermented by Aspergillus oryzae and Rhizopus oligosporus Diah Ratnaningrum; Thelma Agustina Budiwati; Tri Darsini; Panji Cahya Mawarda
Journal of Tropical Biodiversity and Biotechnology Vol 3, No 1 (2018): April
Publisher : Universitas Gadjah Mada

The suitability of corn kernel as raw material to produce miso fermented by rice-koji containing Aspergillus oryzae and Rhizopus oligosporus has been investigated. The optimization was conducted on two important factors in miso production namely mold composition in rice-koji and salt concentration. The mold composition was prepared by inoculating the spores of 2% A. oryzae, 2% R. oligosporus, and 2% the mixture of both in a ratio of 1:1, 2:1, and 1:2 (v/v) into different rice media. The mold composition was optimized to produce rice-koji with high α-amylase and protease activity. Different NaCl concentrations of 10%, 15%, and 20% were subjected to optimization process and added to each mixture after five days of fermentation. The salt concentration was also optimized to produce corn kernel miso with high glucose and high dissolved protein concentration. The result showed that rice-koji containing A. oryzae and R. oligosporus in the ratio of 1:1 had the highest α-amylase and protease activity of 0.42 U/mL and 0.45 U/mL respectively. In addition, the presence of 10% NaCl in corn kernel miso fermented by A. oryzae and R. oligosporus in the ratio of 1:1 exhibited the highest glucose and dissolved protein concentration of 0.64 mg/mL and 8.80 mg/mL respectively. The optimized corn kernel miso by A. oryzae and R. oligosporus in the ratio of 1:1 with 10% NaCl was subjected to nutrient content analysis and compared to the result before the corn kernel was fermented. The nutrient content analysis showed nutrient enhancement after corn kernel was fermented and transformed into a miso. Glucose, dissolved protein, and fat content increased 6.74, 1.34, 7.63 times respectively. This study concludes corn kernel could be utilized to produce a novel corn kernel miso for dietary diversification and for improving nutritional and health status.

Filogenetik Jenis-jenis Annonaceae dari Jawa Timur Koleksi Kebun Raya Purwodadi Berdasarkan Coding dan Non-coding sekuen DNA Dewi Ayu Lestari; Rodiyati Azrianingsih; H. Hendrian
Journal of Tropical Biodiversity and Biotechnology Vol 3, No 1 (2018): April
Publisher : Universitas Gadjah Mada

Annonaceae species of Purwodadi Botanic Garden collections from East Java encountered problems in species identification based on morphological characters. In addition, the unavailability of molecular data to support identification based on morphological characters hinders accurate identification of species. The aim of this research is to discern phylogenetic of Annonaceae species from East Java collections of Purwodadi Botanic Garden based on coding and non-coding sequence DNA. Annonaceae species used in this study are 30 species, includes outgroup from family of Magnoliaceae. Materials for DNA analysis were young leaves which were processed through DNA extraction, DNA amplification through PCR technique, DNA sequencing and data analysis with Maximum Likelihood (ML), Maximum Parsimony (MP) and Neighbour-Joining (NJ) method analysis. The results show that phylogenetic tree is divided into two subfamilies, i.e. Annonoideae and Malmeoideae. The topology of phylogenetic tree from three DNA molecular marker shows that non-coding sequence DNA (trnL-F molecular marker) has the best grouping of relationship and be able to explain the relationship between species of Annonaceae than rbcL and matK molecular marker as coding sequence DNA. Bootstrap value of trnL-F tree is very weak to high (36-100%) while rbcL is very weak to weak (17-63%) and matK tree is high (98-99%).

Isolation and Identification of Endophytic Fungi from Leave and Stem of Calopogonium mucunoides Dian Fitriarni; Rina Sri Kasiamdari
Journal of Tropical Biodiversity and Biotechnology Vol 3, No 1 (2018): April
Publisher : Universitas Gadjah Mada

Thirty-seven isolates of endophytic fungi were isolated from leaves and stems of Calopogonium mucunoides collected from PTPN PTPN XII (Persero) Rubber Plantation, Klatakan, Kecamatan Tanggul, Kabupaten Jember, Jawa Timur. All isolates were identified based on morphological characteristics using the light microscope. The 37 isolates of endophytic fungi are members of Deuteromycota and Basidiomycota and classified to genera Phoma, Phomopsis, Corynespora, Rhizoctonia, Helicosporium, Curvularia, Torulomyces, Gliocladium, Gloeosporium, Acremonium, Tripospermum, Aureobasidium, Colletotrichum, Humicola, Fusarium, Sclerotium, and sterile hyphae.

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