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INDONESIAN JOURNAL OF PHARMACY

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Published by Universitas Gadjah Mada

ISSN : 23389427 EISSN : 23389486 DOI : -

Core Subject : Health,

Medicine & Pharmacology

Indonesian Journal of Pharmacy (ISSN-e: 2338-9486, ISSN-p: 2338-9427), formerly Majalah Farmasi Indonesia (ISSN: 0126-1037). The journal had been established in 1972, and online publication was begun in 2008. Since 2012, the journal has been published in English by Faculty of Pharmacy Universitas Gadjah Mada (UGM) Yogyakarta Indonesia in collaboration with IAI (Ikatan Apoteker Indonesia or Indonesian Pharmacist Association) and only receives manuscripts in English. Indonesian Journal of Pharmacy is Accredited by Directorate General of Higher Education (DGHE) DIKTI No. 58/DIKTI/Kep/2013.

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Articles 7 Documents

Search results for , issue "Vol 13 No 1, 2002" : 7 Documents clear

A Preformulation of a Water Soluble Furosemide Dosage Form Syukri, Yandi; Yuwono, Tedjo; Hakim, Lukman
Indonesian Journal of Pharmacy Vol 13 No 1, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Solid dispersion system of water-insoluble furosemide in polyvinylpirolidon (PVP) was prepared by a solvent method in various ratios of 1 : 1, 1 : 3, 1 : 5, 1 : 7 and 1 : 9 of the drug and PVP, respectively in order to improve furosemide solubility and dissolution. The improvement of furosemide solubility was studied by a solubility method in a shaking waterbath. The solubility test showed that various concentrations of PVP and temperature gave statistically significant increased of furosemida solubility (P < 0,05). The dissolution study of furosemide solid dispersion system was done using a dissolution tester at the rotation rate of 50 rpm. Furosemide concentration released was determined spectrophotometrically using a UV spectrofometer. This test showed a significant increased of furosemida solubility (P < 0,05), but with a prolonged of releasing time.Key Word : solid dispersion, solubility, dissolution, bioavailability.

OPTIMATION OF CALLUS GROWTH MEDIUM OF Vitex Trifolia IN IN-VITRO CULTURE Andayana Puspitasari; CJ. Soegihardjo
Indonesian Journal of Pharmacy Vol 13 No 1, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

A research has been done to optimize of callus growth medium of Vitex trifolia plants in in -vitro culture. These recent years, people start searching new drugs from plants. Vitex trifolia or Legundi has been used to cure swell and as a carminative. The last research reported that this plant contains viteosin-a and vitexicapin which can used as tracheo-spasmolytic. The aimed of this research was to find the sterilization method and to measure the callus growth from leaf explant planted in Murashige-Skoog (MS) solid medium in many combinations of 2,4 Dichlorophenoxy acetic acid (2,4D) and kinetin, afterwards the compounds content in callus and in original plant was compared. The leaf of Vitex trifolia which had been collected from Pharmaceutical Biology department,. faculty of Pharmacy garden was washed in water stream and sterilized by developing into solution of mercury chloride and with tween 80.(as the wetting agent). Using Laminar air flow the explant was planted aceptically in MS solid medium. Culture vessels were placed in a cabinet at 25 3 C, and radiated 16-hour daily using TL lamp (40 watts). After 25-30 days, the callus were subcultured, then after 14-16 days the subculture callus were collected and dried at 50-80C. The dried callus and dry powdered leaf of Vitex trifolia, were macerated with chloroform-methanol (1: 1), then the concetrated extractan was applied on a silica gel GF 254 plate and eluated with chloroform-etil acetate (10: 1). The optimal of sterization method was developing explants in solution of 0,05 % mercury chloride and two drops of tween 80 per 100 ml mixture, for 10 minutes. The optimal combination of 2,4D and kinetin for growing callus in growth MS solid medium was 1 mg/L: 1 mg/L. The Chromatograms show that the chemical constituents in callus are not influenced by variation of growth fertilizer agent in medium plant and the chromatogram of secunder metabolites fund in original plant as well as in callus had no significantly different.Key words: in-vitro culture, Vitex trifolia

PRODUCTION OF QUININE ALKALOID BY SOME ENDOPHYTIC MICROBES WITH ADDITION OF INDUCER SUBSTANCES (Studies on Endophytic Microbes of Cinchona sp. plants (2)) Simanjuntak, Partomuan; Parwati, Titi; ., Bustanussalam; Prana, Titik K.; Shibuya, Hirotaka
Indonesian Journal of Pharmacy Vol 13 No 1, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Endophytic microbes have been known to be potential as the sources of active compound for medicines. Optimization of medium, including of inducer addition, is necessary to obtain optimum products. This study, therefore, was aimed to investigate the effect of Cinchona succirubra bark powder addition in the production of quinine compound by endophytic microbe growing in two different liquid media. Endophytic microbe isolated from Cinchona sp. were shaker incubated in Potato Dextrose Broth (PDB) and Phoma media with the addition of bark powder of C. succirubra for 3 days. Quinine production was analyzed with High Performance Liquid Chromatography (HPLC). The result showed an increasing of quinine production for each microbes. Highest increasing of quinine production (2.2 folds) was yielded by mold culture (J3P1) grown in PDB medium.Keywords: inducer, quinine, endophytic microbe, Cinchona succirubra

TOXICITY OF THE AQUEOUS EXTRACTS FROM THE FRUITING BODY OF GANODERMA SP. BY BRINE LETHALITY TEST OF Artemia salina Leach. Rumiyati .; Sismindari .; S.M. Widyastuti
Indonesian Journal of Pharmacy Vol 13 No 1, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

The level of toxicity of aqueous extract from Ganoderma sp. fruiting body isolated from different plant media was determined in this experiment using Brine shrimp lethality test (BST) on Artemia salina Leach. The influence of media on the toxicity level of aqueous extract from Ganoderma sp. fruiting body was determined in this experiment. Ganoderma sp. and G. Lucidum were grown on three flamboyant, (Delonix regia Bojer ex Hook Rafin), sengon (Paraserianthes falcataria (L) Nielsen) and coconut(Cocos nucifera Linn.) plant media. The aqueous extracts were prepared from fruiting body using 0.02 M phosphate buffer pH 7.2. The toxicity level of these aqueous extract were then screened using the BST method. The level of the toxicity was determined by LC50. The results indicated that the aqueous extracts of Ganoderma sp. and G. lucidum isolated from flamboyant, sengon and coconut plant media were influenced by plant media. Ganoderma sp. was isolated from flamboyant plant media possesed the highest toxicity (LC50 480 g aqueous extract/ml), then followed by Ganoderma sp. isolated from sengon plant media (LC50 770 g aqueous extract /ml) and coconut (LC50 1040 g aqueous extract /ml). G. lucidum from coconut plant media possesed the highest toxicity (LC50 660 g aqueous extract /ml), followed by G. lucidum were isolated from sengon plant media (LC50 1100 g aqueous extract /ml) and flamboyant (LC50 1970 g aqueous extract /ml). It was suggested that G.lucidum having highest toxicity might produce compounds having antitumor activity.Key words: Ganoderma sp., aqueous extracts, toxicity .

THE INFLUENCE OF AQUEOUS EXTRACT OF CORIANDRI (Coriandrum sativum L) FRUCTUS TO BLOOD GLUCOSE LEVEL IN GLUCOSE- PRE LOADED RATS Agung Endro Nugroho
Indonesian Journal of Pharmacy Vol 13 No 1, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Coriander contains some active compounds capable of decreasing the level of blood glucose. Moreover, aqueous extract of coriander (Coriandrum sativum L.) fruits has insulin-releasing and insulin-like activities. This research has been performed to obtain information on the effect of aqueous extract of coriander fruits on the blood glucose level in glucose-preloaded rats. The study was conducted by employing a complete random design in male Wistar rats (age 2-2,5 months, 150-250g).After the design treatments, the blood samples were collected (100-200 ul) at certain time interval. The result of each group were compared and analysed using the Mann-Whitney non-parametric method (95%). The result showed that low doses (0.014 and 0.07 % w/v) of aqueous extract of coriander fruits were unable to decrease significantly (p>0.05) the blood glucose level in glucose-preloaded glucose. On the other hand, high doses, 0.35 and 1.75 % w/v, were capable to decrease significantly (p<0.05), which are 22.11 ± 3.09 and 18.41 ± 4.88 %, respectively. Nevertheless, in the present study, the highest hypoglycaemic effect of aqueous extract of coriander fruits still lower than that of tolbutamide (p<0.05).Keywords: coriander, hypoglycaemic, glucose, tolbutamide

IDENTIFICATION OF THE VIRUS DENGUE–3 EPITOPE’S IMMUNODOMINAN USING THE SYNTHETIC PEPTIDE Nirwati, Hera; ., Sutaryo; Wahyono, Djoko
Indonesian Journal of Pharmacy Vol 13 No 1, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Dengue virus infection has been known as an important health problem in many tropical countries, because of increasing number of patients, expansion of epidemic areas and emergence of severe clinical manifestations. Dengue virus consists of 3 structural and 7 nonstructural proteins. The major virion surface protein, the envelope protein, is the most important antigen with regards to virus biology and humoral immunity. Synthetic peptide derived from the envelope protein gene sequence can be used to identify region of the envelope protein that elicits antibodies. One hundred and sixty-one synthetic peptides were synthesized based on sequence of protein envelope dengue-3 virus published by Osatomi and Sumiyoshi. Each peptide consists of 15 amino acids with the last 12 amino acids overlapped. Peptide synthesizer, auto-spot robot ASP 222 (Abimed), was used for synthesis using solid spot method developed by Ronald Frank. Synthetic peptides attached to nitrocellulose membrane were used for spot immunoassay of 6 normal human sera, 6 dengue infected human sera and 6 non dengue-infected. Nine peptides were specific for dengue infected sera and these might be candidate immunodominat epitopes of dengue virus-3. They were EGLSGATWVDVVLEH (amino acids number 13-27), VCKHTYVDRGWGNGC (91-105),SIEGKVVQHENLKYT (124-138), TVHTGDQHQVGNETQ (142-156), TLGLECSPPTGLDFN (178-192), KGEDAPCKIPFSTED (325-339), PFSTEDGQGKAHNGR (334-348), GARRMAILGDTAWDF (406-420) and KIGIGVLLTWIGLNS (454-468). Based on spot immunoassay, 11 peptides were synthesized and used them for ELISA of 15 normal sera, 22 non-dengue infected sera and 37 dengue sera. Peptides BTLDDIELQKTEATQLA, BPFSTEDGOGKAHNGR and BKKEEPV NIEAEPPFG showed significantly different in their reactivities to the three groups. Combinations of two peptides were used for ELISA. Only one of them (BKGEDAPOKIPFSPED and BRMAILGD TAWDFGSV) showed significantly different in their reactivi- ties to the three groupsKey words: synthetic peptide, envelope protein dengue-3, spot immunoassay

ESTIMATION OF PROTEIN CONCENTRATION IN FOOD BY TOTAL NITROGEN AND AMINO ACID ANALYSES Sumarno .; Sri Noegrohati; Narsito .; Iip Izul Falah
Indonesian Journal of Pharmacy Vol 13 No 1, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Since protein is the principal constituent of biological organs, a continuous supply is needed in food for growth and repair. Protein quality in food is determined by its assortment of amino acids, whether it is essentially needed or not. The aim of this study is to develop an accurate and precise simple method of protein estimation for food and feed. The validity of simple protein estimation by multiplying the N-total concentration with a constant factor: of 6.25, should be clarified. Protein could be estimated more specifically by summing up the determined amino acids. In this study, the amino acids of some substantially high protein food were analysed by HPLC-ninhydrin, HPLC-o-phthalaldehyde, and HPLC-dansyl techniques, by gas chromatograph after derivation with TFA-butanol, and by formol titration. Method based on HPLC-ninhydrin techniques shows significant correlation (r=0-9992) with its amino acids content and N-total content. The protein as amino acids - N-total conversion factor obtained was 6.95 for N-total content less than 15.5%, and 8.59 for N-total content higher than 15.5%.Key words: food, protein and analytical methods

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Kab. sleman,

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