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Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
Arjuna Subject : -
Articles 12 Documents
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Search results for , issue "Vol 2, No 3 (2012)" : 12 Documents clear
Binding Inhibition Between Igf1r and Igf1 by Catechin of Black Tea Firdausi, Lina; Indra, M Rasjad; ., fatchiyah
Journal of Tropical Life Science Vol 2, No 3 (2012)
Publisher : Journal of Tropical Life Science

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Abstract

The natural compound of black tea is used as an alternative of obesity therapies in the world; particularly, the catechin family in tea leaves which has bioactive compounds such as EC, EGC and EGCG. Their bioactivity contributes to inhibit the ligand of Insulin-Like Growth Factor I Receptor (Igf-1r) binding-region to Igf-1 protein. To elucidate the inhibiton of Igf-1 expression and proliferating of Rattus norvegicus strain wistar adipose cell using black tea solution. The research used Rattus norvegicus strain wistar. After a 90-day treatment, the adipose tissues were picked up from the viscera of each experimental animal, and then the adipose tissues were embedded by paraffin. The paraffin sections were determined through immunohistochemistry with anti-Igf-1 antiserum, and were also analyzed through hematoxylin-eosin. A protein sequence of Igf-1, Igf-1r, and 3D structure of EC, EGC and EGCG from Gene Bank sites were used during in silico analysis. The sequences were aligned by BLAST program to identify the conserve and variable domain of IGF-1 protein isoforms. The 3D structures of IGF-1 and IGF-1R were constructed using Phyre program. The ligand among the 3D structures of IGF-1, IGF-1R and catechin compounds were analyzed using Hex 5.1 docking program. The data showed that the Igf-1 expression of adipose cells was reduced at 0,03 g/ml BTS and 0,045 g/ml BTS treatments. The result of BLAST analysis showed that IGF-1 (a, b, c, and d) isoforms conserved a domain from amino acid no 22 until 134; and this region was a variable region. The EGCG bound L1 domain of IGF-1R with E-total -235.3 KJ/mol which was lower than EC (-208,4 KJ/mol) and EGC (-142 KJ/mol). The total energy of IGF-1 (a, b, c, but not d isoform) which interacted with EGCG was around -223.7 KJ/mol, EC is -205.6 KJ/mol and EGC was -191.7 KJ/mol. However, EC, EGC and EGCG was only able to prevent the interaction between the L1 of IGF-1R with IGF-1 protein, but not the opposite.Keywords: Adipose cell, black-tea, proliferation, catechin, IGF-1, IGF-1R, inhibition, docking
Growth Adaptation of Two Indonesian Black Rice Origin NTT Cultivating In Organic Paddy Field, Malang-East Java Budiman, Budiman; Arisoesiloningsih, Endang; Edi Wibowo, Ratno Bagus
Journal of Tropical Life Science Vol 2, No 3 (2012)
Publisher : Journal of Tropical Life Science

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Abstract

This research is aimed to study the growth adaptation of two Indonesian black rice NTT in origin which is cultivating in organic paddy field in Malang - East Java and its problems during a planting period. A descriptive exploratory research was conducted in two organic rice fields in Kepanjen District, Malang Regency. Those fields were grown with two different varieties of black rice NTT in origin namely, Laka and Woja Laka. Each rice field was recorded periodically. The growth was including the plant height, biomass, density of tiller, panicle and spikes, dry seed weight and the problem correlated to pest and climates limitation. The results showed that Woja Laka and Laka black rice varieties slightly gave a different growth pattern. Laka variety was taller than Woja Laka, i.e. the Laka could reach 200 cm of height, while the Woja Laka was maximum 150 cm of height. However, the density of Woja Laka tiller and its panicle were almost double than the Laka ones. The dry seed weight and spikes number of Woja Laka were much higher than in Laka ones. In terms of organic farming system, Laka rice seemed to be more sensitive towards the strong wind conditions and bird or rat attacks rather than Woja Laka. Both pests attacked on both black rice along growing period, but rat preferred to attack vegetative part, otherwise bird strongly disturbed during generative period. Key words: black rice, growth, Laka, Woja Laka, organic paddy field

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