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Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
Arjuna Subject : -
Articles 15 Documents
Search results for , issue "Vol. 8 No. 3 (2018)" : 15 Documents clear
Date Fruit Extract (Phoenix dactylifera, Ajwa) Modulates NK Cells and TNF- Alpha in DMBA-Induced Mammary Cancer Sprague-Dawley Rats Rehab Mohammed Mustofa Elhemeidy; Diana Lyrawati; Edi Widjajanto
Journal of Tropical Life Science Vol. 8 No. 3 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.03.04

Abstract

Cellular immune system is one of anti-tumor target development. NK cells, one of major components of antitumor innate immune responses, are frequently decreased functionally during breast cancer progression, whereas TNF-alpha changes from anti-tumor into pro-tumorigenesis and its level increases with advancing cancer. In this study, we investigated the immunomodulatory effects of Ajwa date fruit extract to determine whether it stops the progression of breast cancer. Using rats induced with single dose 20 mg DMBA subcutaneously, we examined whether administration of Ajwa date fruit (Phoenix dactylifera) extract (at 400, 800 mg/kg body weight/d) post-DMBA induction for 30 days modulate NK cells, TNF-alpha, and development of breast cancer. ELISA, FACS, immunohistochemistry, and histologic observation were employed. Compared to positive control group (DMBA-induced only), we showed that the Ajwa date fruit extract normalize the level of circulatory CD161 NK cells and breast tissue TNF-alpha, cell size and proliferation, and improve overall survival rates. Ajwa date fruit extract may be used to modulate NK cells and TNF-alpha against progression of breast cancer. Further studies are required to develop date fruit extract as anti-cancer in human, whether as single or adjunct therapy to standard management of breast-cancer.
New Record of Marine Parasitic Isopods (Family, Corallinidae) from South Andaman, Andaman Islands, India Pathan Anil; Tarun Das; Kaderambil Arjunan Jayaraj
Journal of Tropical Life Science Vol. 8 No. 3 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.03.12

Abstract

Parasitic isopods are causing problems to the worldwide fishery resources. The studies on parasitic isopods are very scanty in Andaman Islands. So here we provided information on the distribution of two species of parasitic isopods of fishes from Junghlighat fish landing center of South Andaman. The two marine parasitic isopods species are Argathona rhinoceros (Bleeker 1857) and Argathon amacronema (Bleeker 1857). They found to infest the host marine fishes Epinephelus coioides (Hamilton 1822) and Epinephelus tauvina (Forsskal 1775) respectively. These two species were collected from the nostril cavity of the individual host fishes. The morphological characteristics of two specimens are described in detail. Prevalence of corallinids provides additional information to update the check list of isopods from Andaman and Nicobar Islands.
In vitro Studies on Pollen Viability, Pollen Germination and Pollen Tube Growth of Hornstedtia conoidea Ridl. – a Philippine Endemic Ginger Species Noe Polo Mendez; Florfe Macabudbud Acma
Journal of Tropical Life Science Vol. 8 No. 3 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.03.13

Abstract

Zingiberaceous plants are predominantly propagated through underground rhizomes and seeds. In this study, the viability of pollen, rate of pollen germination and length of pollen tube of the Philippine endemic Hornstedtia conoidea were examined. Four petri plates containing pollen samples were prepared, of which two petri plates were used immediately after the collection, while the other two petri plates were stored for one week. The determination of pollen viability was made employing stain tests using IKI (iodine + potassium iodide) solution and safranin and measured immediately after the collection and after one week. Likewise, pollen germination test was carried out in vitro on agar medium and measured after 24 hours and one week of incubation. Data revealed that pollen viability was higher in the samples which were sown immediately with safranin with percentage pollen viability of 92.43%, while IKI test was lower with 89.36%. On the other hand, the pollen stored for one week measured 47.29% for safranin test and 33.14% for IKI test. The percentage germination of pollen after 24 hours was 72.65%, while 11.13% after one week. The pollen samples which were subjected for tube growth were examined for two weeks and gave positive results. Overall, it can be concluded that H. conoidea pollen can successfully be collected and stored for certain time. Knowledge regarding the pollen viability, pollen germination and pollen tube growth of this species will give practical benefit for plant breeding and conservation purposes, since H. conoidea is found in the wild, and as of now, the species has been recorded only in the Philippines.
Antimicrobial Production by an Actinomycetes Isolated from The Termite Nest Ni Putu Ratna Ayu Krishanti; Deni Zulfina; Bramantyo Wikantyoso; Arpiwi Zulfitri; Sulaeman Yusuf
Journal of Tropical Life Science Vol. 8 No. 3 (2018)
Publisher : Journal of Tropical Life Science

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Abstract

Actinomycetes are Gram positive bacteria that have been detected in diverse ecological niches. Their member species are known to be a main source of various bioactive compounds. The discovery of Actinomycetes from diverse and unexplored resources has also been linked to increased opportunities to obtain novel bioactive compounds. Insect nest material is being investigated as a new source of novel antimicrobial producing Actinomycetes, which could be harnessed for therapeutic potential. A total of 10 Actinomycetes isolates were collected from the nest of Nasutitermes sp. in Pananjung Pangandaran Nature Reserve. These isolates were evaluated for antimicrobial activity against the challenge bacteria (Eschericia coli, Staphylococcus aureus, Bacillus subtilis, Serratia Marcescens) and fungi (Fomitopsis palustris, Fusarium oxysporum, Trichoderma viridae) by dual culture method. The result revealed that several isolate were active against fungi and bacteria. Isolate Pn-TN2 showed the highest level of antibacterial inhibition and the highest antifungal inhibition with Inhibition Rate value more than 80%. By morphological and 16S rRNA gene sequence analysis strongly suggested that the isolate Pn-TN2 belonged to Streptomyces prasinopilosus. We suggested that termite nest is a potential source of bioactive strains of cultivable Actinomycetes for future biotechnological needs.
Ameliorative Effect of Infused Watercress on Rat Galactopoiesis following Maternal Separation Rahadian Yudo Hartantyo; Ucik Erlitaningsih; Jeremia Gerald; Indah Permata Sari; Laksmindra Fitria; Mulyati Mulyati
Journal of Tropical Life Science Vol. 8 No. 3 (2018)
Publisher : Journal of Tropical Life Science

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Abstract

Galactopoiesis is the maintenance of milk production. The process that regulated primarily by prolactin hormone. The research was conducted to evaluate galactopoiesis activity by measuring prolactin level and milk production continuity of lactating rats after oral administration of infused watercress. Twenty lactating rats were randomly divided into five groups (two control groups and three variations of infused watercress dose groups). Serum prolactin was measured using enzyme-linked immunosorbent assay. Milk yield was measured using indirect milk measurement method by Sampson and Jansen. Milk protein level was measured using Kjedahl method. The treatment of infused watercress (10 g/kg body weight), started from postnatal day 3 until 12, indicated ameliorative effect on rat galactopoiesis by increasing milk yield and prolactin levels, but not milk total protein.

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