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Jurnal Keselamatan Radiasi dan Lingkungan
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Articles 5 Documents
Search results for , issue "Vol 1, No 2 (2016): Desember 2016" : 5 Documents clear
Efek Induksi Mutasi Radiasi Gamma 60Co Pada Pertumbuhan Fisiologis Tanaman Tomat (Lycopersicon esculentum L.) Gusti Ngurah Sutapa; I Gde Antha Kasmawan
Jurnal Keselamatan Radiasi dan Lingkungan Vol 1, No 2 (2016): Desember 2016
Publisher : Pusat Teknologi Keselamatan dan Metrologi Radisasi - BATAN

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Abstract

Hampir seluruh jenis masakan di Indonesia menggunakan tomat (Lycopersicon esculentum L.) sebagai bahan dasar pembuatannya. Nilai gizi yang terkandung dalam tomat juga cukup tinggi, karena terdapat sejumlah kandungan vitamin yang diperlukan oleh tubuh manusia. Selain itu, tomat menjadi tanaman unggulan nasional komoditas hortikultura dan prioritas utama pada sejumlah provinsi di Indonesia. Demikian banyak manfaat dari tomat mengindikasikan bahwa produktivitas tomat harus segera ditingkatkan. Salah satu peningkatan dari segi kualitas dapat dilakukan dengan cara induksi mutasi dengan radiasi gamma Co-60. Induksi mutasi adalah perubahan genetik yang disebabkan oleh usaha manusia, salah satu caranya yaitu dengan bahan radioaktif. Radiasi gamma Co-60 dari pesawat IRPASENA dipaparkan pada biji tomat dengan perlakuan dosis 50, 100, 150, 200 dan 250 Gy. Selanjutnya dilakukan pengukuran terhadap pertumbuhan fisiologis meliputi lebar daun, tinggi tanaman, jumlah buah dan berat basah buah tomat dari minggu-1 hingga panen. Hasil penelitian menunjukan kurva pertumbuhan fisiologi tanaman tomat sesuai dengan kurva sigmoidal pertumbuhan tanaman. Pertumbuhan fisiologis tanaman tomat optimal pada dosis radiasi gama Co-60 100 Gy. Pada dosis optimal tersebut pertumbuhan fisiologis tanaman tomat diketahui yang terbaik (unggul) dibandingkan pada dosis di bawah dan di atas 100 Gy maupun kontrol. Almost all types of cuisine in Indonesia are using tomatoes as the base material of manufacture. The nutritional value contained in tomatoes is also quite high, because there is a number of vitamin content required by the human body. In addition, the tomatoes in plants featured national horticultural commodity and priority on a number of provinces in Indonesia. So many benefits of tomatoes indicates that the productivity of tomatoes should be improved. One improvement in terms of quality can be done by means of mutation induction with gamma radiation of Co-60. Induction of mutations are genetic changes caused by human effort, one of them is by using radioactive materials. Gamma rays of Co-60 from the IRPASENA facility was exposed to tomato seeds at doses of 50, 100, 150, 200 and 250 Gy. And then measurements were conducted on the physiological growth of leaf width, plant height, number of fruit and wet weight of tomatoes from week 1 until harvest. The results showed a growth curve of tomato is in accordance with sigmoidal plant physiological growth curve. Optimal physiological growth of tomato plants was obtained at dose of gamma radiation of 100 Gy. At this optimal dose physiological growth of tomato plants is the best (superior) than in doses below and above 100 Gy and control.
Determination of Carbofuran on Hydrophilic Interaction Liquid Chromatography Using Tsk Gel Amide 80 As Stationary Phase A. Kurnia; L.W. Lim; T. Takeuchi
Jurnal Keselamatan Radiasi dan Lingkungan Vol 1, No 2 (2016): Desember 2016
Publisher : Pusat Teknologi Keselamatan dan Metrologi Radisasi - BATAN

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Abstract

The hydrophilic interaction liquid chromatography (HILIC) coupled to environmental friendly capillary liquid chromatography was employed to investigate retention behavior of carbofuran. Aim of this research is to investigate retention behavior of carbofuran using TSK gel amide 80 as stationary phase. Several condition was conducted to investigate retention behavior of carbofuran such as comparison study of TSK gel amide 80 with other polar column, comparison study retention behavior of carbofuran on various system wavelength, water content effect in HILIC mode, effect of buffer concentration on HILIC mode, and analytical performance. Results showed that TSK gel imidazole was superior compare to other polar stationary phase on determine carbofuran, wavelenght 251 and 254 nm was resulting higher absorbance for carbofuran than others, increase of water content on mobile phase was decrease the retention time, also increase buffer salt concentration was decrease the retention time and according to analysis performance that is the accuracy was 101±10,1, the LOD 0.66 ppm while LOQ 2.22 ppm. As conclusions that TSK gel amide 80 was offering good determine on carbofuran even using capillary liquid chromatography with 10 cm length of column.
Distribusi Radionuklida 137Cs di Air dan Sedimen Pulau Pari Kepulauan Seribu Jakarta Herni Kusuma; Mohammad Nur Yahya; Sri Yulina Wulandari
Jurnal Keselamatan Radiasi dan Lingkungan Vol 1, No 2 (2016): Desember 2016
Publisher : Pusat Teknologi Keselamatan dan Metrologi Radisasi - BATAN

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Abstract

Radionuklida 137Cs yang memiliki waktu paruh panjang yaitu 30 tahun telah tersebar dan terendapkan ke dalam sedimen di perairan laut sehingga perlu diketahui persebarannya. Penelitian mengenai distribusi radionuklida 137Cs di Pulau Pari penting untuk dilakukan karena perairan tersebut terletak di Kepulauan Seribu bagian selatan yang berdekatan dengan Teluk Jakarta. Banyak buangan limbah domestik dan industri yang berasal dari sungai-sungai yang mengalir ke muara Teluk Jakarta. Selain itu adanya aktivitas labotarium BATAN Serpong juga memungkinkan mengalirkan limbahnya ke Sungai Cisadane. Penelitian ini bertujuan untuk memperoleh nilai dan sebaran 137Cs di kolom air dan sedimen dasar pada perairan Pulau Pari serta hubungannya dengan karakter umum sedimen yaitu ukuran butir dan kandungan karbon serta pola arus. Pengambilan sampel air dan sedimen dilakukan pada tanggal 26 Maret 2016 masing-masing sebanyak 7 stasiun. Analisa pengukuran kandungan 137Cs dilakukan di PTKMR-BATAN. Hasil penelitian menunjukan aktivitas 137Cs di air berkisar 0,01-1,16 mBq/l dan di sedimen berkisar 0,265-0,653 Bq/kg. Hasil pemodelan arus menunjukan sebaran 137Cs di perairan dipengaruhi oleh arah dan kecepatan arus. Sebaran 137Cs di sedimen dasar perairan Pulau Pari kurang memiliki hubungan yang signifikan dengan ukuran butir sedimen dan kandungan karbon. The 137Cs Radionuclide which has a long half-life (30 years), has been dispersed and deposited into ocean areas, so its spread needs to be known. Research for distribution of 137Cs radionuclide in Pari Island is indispensable because it is located in the southern part of Seribu Islands waters located adjacent to Jakarta Bay. There are much domestic and industrial sewage coming from the rivers to Jakarta Bay. Beside there are some possibility of radiological substances entry derived from labotary activities at BATAN-Serpong through Cisadane River.These study was aimed to get the value and distribution of 137Cs in the seawater and sediments also their relation to the general character of the sediment that were grain size and carbon content, as well as current patterns. Seawater and bottom sediment sampling were conducted on March 26, 2016 respectively of 7 station. Analysis of the samples in order to measure the concentration of 137Cs were conducted in PTKMR-BATAN. The results showed the activity of 137Cs in the water ranging from 0.01 to 1.16 mBq/l and in sediments ranging from 0.265-0.653 Bq/kg. Flow modeling results indicate that the distribution of 137Cs in seawater were affected by current direction and speed. Activities and distribution of 137Cs in bottom sediments of Pari Island waters were less affected by grain size and carbon content.
Cover; Editor Jurnal Keselamatan Radiasi dan Lingkungan
Jurnal Keselamatan Radiasi dan Lingkungan Vol 1, No 2 (2016): Desember 2016
Publisher : Pusat Teknologi Keselamatan dan Metrologi Radisasi - BATAN

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Abstract

Jurnal Keselamatan Radiasi dan LingkunganPusat Teknologi Keselamatan dan Metrologi RadiasiBadan Tenaga Nuklir Nasional
Pendeteksian Ekspresi Biomarker MNK Secara Semi Kuantitatif dan Kuantitatif Pada Kanker Serviks Sebelum Respon Kemoradioterapi Teja Kisnanto; Rina Tri Wardani; Budiningsih Siregar; Mellova Amir; Setiawan Soetopo; Irwan Ramli; Tjahya Kurjana; A Andrijono; Bethy S Hernowo; Maringan DL Tobing; Devita Tetriana
Jurnal Keselamatan Radiasi dan Lingkungan Vol 1, No 2 (2016): Desember 2016
Publisher : Pusat Teknologi Keselamatan dan Metrologi Radisasi - BATAN

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Abstract

Kanker servik merupakan penyakit kanker yang umum dijumpai pada wanita yang disebabkan oleh virus HPV (Human Papilova Virus). Tujuan penelitian ini adalah untuk mengetahui hubungan ekspresi protein MNK (Mitogen Activated Protein Kinase) pada penderita kanker serviks sebelum tindakan pengobatan terhadap respon kemoradioterapi. Sampel uji yang digunakan adalah sediaan mikroskopis hasil biopsi jaringan kanker dari penderita kanker serviks stadium lanjut (IIB-IIIB) sebanyak 20 sampel. Metode yang digunakan adalah metode imunohistokimia dengan menggunakan biomarker MNK pada biopsi jaringan kanker serviks. Ekspresi protein MNK yang positif ditandai dengan warna coklat tua yang terdapat pada inti sel. Respon kemoradioterapi diperoleh dari RSUPN Dr. Cipto Mangunkusumo Jakarta dan RS Hasan Sadikin Bandung. Hasil penelitian menunjukkan nilai IRS (Imuno Reaktif Score) protein MNK pada grup respon kemoradioterapi baik lebih tinggi dibandingkan grup respon kemoradioterapi buruk dan tidak ditemukan adanya hubungan IRS protein MNK dengan respon kemoradioterapi. Sedangkan hubungan ekspresi MNK terhadap respon kemoradioterapi menunjukkan adanya korelasi perbedaan grup respon kemoradioterapi antara ekspresi protein MNK negatif dan ekspresi protein MNK positif. Cervical cancer is a cancer that common in women caused by HPV (Human Papilova Virus). The purpose of this study is to determine the relationship MNK protein expression (Mitogen-Activated Protein Kinase) in patients with cervical cancer before chemoradiotherapy treatment. Sample used was the preparation of microscopic cancer tissue biopsies from patients with advanced cervical cancer (IIB-IIIB) is 20 samples. The method used is immunohistochemistry using MNK biomarkers in cervical cancer tissue biopsies. MNK positive protein expression marked with dark brown color that is contained in the cell nucleus. Chemoradiotherapy response obtained from RSUPN Dr. Cipto Mangunkusumo and Hasan Sadikin Hospital in Bandung. The results show the value of the IRS (Immuno Reactive Score) MNK protein in response to chemoradiotherapy group either higher than the response to chemoradiotherapy group was bad and did not find any relationship IRS MNK protein with chemoradiotherapy response. While the relationship MNK expression responses show a correlation chemoradiotherapy group differences in chemoradiotherapy response between MNK expression negative and MNK expression positive.

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