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International Journal of Biosciences and Biotechnology
Published by Universitas Udayana
ISSN : 23033371     EISSN : 26559994     DOI : -
Core Subject : Science, Social,
Biochemistry, Genetics & Molecular Biology Environmental Science
International Journal of Biosciences and Biotechnology provides a unique venue for publishing original researches in biosciences and biotechnology, and ensures that authors could reach the widest possible audience. It publishes both full-length articles and short communications on all aspects of biotechnology and biosciences
Arjuna Subject : -
Articles 6 Documents
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Search results for , issue "Vol 1 No 2 (2013)" : 6 Documents clear
ZYMOGRAPHY OF EXTRACELLULAR PROTEASES IN Bacillus subtilis Takeko Kodama; Keiji Endo; Katsutoshi Ara; Katsuya Ozaki; Junichi Sekiguchi
International Journal of Biosciences and Biotechnology Vol 1 No 2 (2013)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

In Bacillus subtilis, AprE, Bpr, Epr, Mpr, NprB, NprE, Vpr and WprA have been identifi ed as extracellularproteases. We determined protease activities from the culture supernatant of Bacillus subtilis using a 1Dzymogram gel containing gelatin. The highest protease activities were found at the late stationary phase ofa 75-h culture. To distinguish the proteases, the zymogram patt ern of the wild type was compared with theprotease-defi cient mutants (aprE, bpr, epr, mpr, nprB, nprE, vpr and wprA). Activities of AprE, Bpr, Mpr, andVpr were estimated by gelatin zymography. In addition, for the aprE mutant, the zymogram profi le of Bprwas very diff erent from that of the wild type. This suggested that AprE is involved in processing the Bprprotein.
CLONING OF FLOWERING GENES (WJFLC AND WJFT) IN WASABI (JAPANESE HORSERADISH) AND MONITORING OF FLOWERING RESPONSE WITH THEIR EXPRESSION Masayuki Nozue; Hiroyoshi Kubo; Kiyoshi Yoshida
International Journal of Biosciences and Biotechnology Vol 1 No 2 (2013)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Wasabi (Wasabia japonica) is a commercially important crop in Japan. We isolated a FLC ortholog (WjFLC)and FT ortholog (WjFT) from wasabi. Predicted amino acid sequence encoded by WjFLC and WjFT showed89% and 85% identities with FLC and FT of Arabidopsis, respectively. The expression of WjFLC was high inOctober and reduced in November when fl ower buds are formed in wasabi. On the other hand, expressionof WjFT was not detected in October and was slightly detected in November. Thereafter, WjFT was highlyexpressed later in February. We examined the best condition for initiation of fl ower bud formation undervarious artifi cial environments by monitoring the fl owering response of wasabi using WjFLC and WjFT.
GROWTH PROFILES AND ETHANOL PRODUCTION OF TWO YEAST STRAINS Kluyveromyces marxianus IFO-288 AND Kluyveromyces marxianus IFO-617 DURING FERMENTATION OF SOYBEAN PULP HYDROLYZATE SUBSTRATES Kahar Muzakhar
International Journal of Biosciences and Biotechnology Vol 1 No 2 (2013)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Two yeast strains, K.marxianus IFO-288 and K.marxianus IFO-617, were observed to utilize carbon andnitrogen sources obtained from enzymatically hydrolyzed soybean pulp (hydrolyzate). Under aerobiccondition where 1.5% hydrolyzate was used, the maximum biomass of the two yeast yield reached 30%with the protein contents of 47-53%. The two yeast strains also fermented the soybean pulp hydrolyzateand produced alcohol when anaerobic condition was set. The alcohol production effi ciencies of the K.marxianus IFO-288 and K. marxianus IFO-617were 49% and 59.7%, respectively in the medium containing1.5% hydrolyzate. These values were noticed to decrease for the two strains when the concentration of thehydrolyzate was increased. At the concentrations of 2.5% and 5% hydrolyzate for examples, the alcoholproduction effi ciencies of the K.marxianus IFO-288 became 42.5% and 18.5%, respectively, while 45.6% and20.8%, respectively were recorded in the medium inoculated with K. marxianus IFO-617. At 10% hydrolyzateconcentration, none of the yeast strains grew or produced alcohol, indicating that some inhibitory substancesin hydrolyzates were present in the fermentation medium.
DEVELOPMENT OF IN PLANTA TRANSFORMATION METHOD USING Agrobacterium tumefaciens THAT IS SIMPLE AND EFFICIENT AS WELL AS APPLICABLE TO VARIOUS PLANTS Mineo Kojima
International Journal of Biosciences and Biotechnology Vol 1 No 2 (2013)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

A simple and effi cient in planta transformation method was developed. In the method, meristems of eitherapical or axillary buds of immature plants or apical buds of embryos in imbibed seeds, depending on plants,were inoculated by Agrobacterium tumefaciens after being pricked with a needle. The inoculated plants weregrown to maturation in pots under non-sterile conditions. Transformation was demonstrated by severallines of evidence obtained with mostly the progenies of T1 generation; phenotypic inheritance from T0plants to plants of the following generation, resistance of seed germination to antibiotics, detection of?-glucuronidase activity in transformants of T1 generation, detection of transgene by Southern blot andPCR analyses in T1 generation transformants and rescue from T1 generation transformants of the plasmidscomposed of T-DNA of binary vector and fl anking plant genomic DNA. The diverse species of plants such asbuckwheat (Fagopyrum esculentum), mulberry (Morus alba L.), kenaf (Hibiscus cannabinus), rice (Oryza sativa),wheat (Triticum aestibvum L.), maize (Zea mays), and soybean (Glycine max) were shown to be effi cientlytransformed by our in planta method.
NON-HOST RESISTANCE ACTIVITIES OF Arabidopsis thaliana INDUCED BY METHANOL EXTRACT OF MYCELIA FROM Phytophthora infestans Mohammad Shahjahan Monjil; Shinya Wada; Daigo Takemoto; Kazuhito Kawakita
International Journal of Biosciences and Biotechnology Vol 1 No 2 (2013)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Defense system of a non-host pathosystem involving the model plant Arabidopsis thaliana and methanolextract of mycelia (MEM) elicitor from Phytophthora infestans are presented in this article. A. thaliana leaveswere analyzed for the induction of reactive oxygen species (ROS), hypersensitive reaction (HR) like celldeath as well as defense gene expression by MEM elicitor. MEM elicitor induced O2¯ generation in A.thaliana leaves with dose dependent nature. HR occurred during the elicitation of A. thaliana challengedwith MEM elicitor. Responses of intact A thaliana seedlings to MEM elicitor caused the retardation of growthof seedlings. Both plant biomass and growth of primary roots was markedly reduced by the treatment ofMEM. MEM elicitor induced the expression of defense genes such as pathogenesis related proteins, PR1,PR2, PR4 and PR5, and rbohF encoding a ROS generation. These results suggested that MEM elicitor inducednon-host defense responses in A. thaliana.
MERCURY ACCUMULATION IN GOLD MINE TAILING BY SWEET SORGHUM INOCULATED WITH CHROMIUM UPTAKE ENHANCING RHIZOBACTERIA Desi Utami; Sachiko Takahi; Irfan Dwidya Prijambada
International Journal of Biosciences and Biotechnology Vol 1 No 2 (2013)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Small scale gold mining provides an important source of income for miners in rural communities whereeconomic alternatives are extremely limited. However, it releases mercury which damages the environmentand poses risks to those in the nearby community. Phytoremediation is considered as a simple and costeffective method for the cleanup of heavy metal from contaminated soil. Phytoremediation is a technologythat uses plants to degrade, extract, contain, or immobilize contaminants from soil and water. In particular,phytoextraction is the uptake of contaminants by plant roots and translocation within the plants to shootsor leaves. Contaminants are then removed by harvesting the plants. The objective of this research is toexamine the ability of sweet sorghum and its inoculation using chromium uptake enhancing rhizobacteriafor phytoremediation of gold mine tailing. Two varieties of sweet sorghum were used in this experiment, i.e.FS501, and KCS105. The seed of the sweet sorghum was sown in mercury containing tailing or a mixture ofit with uncontaminated soil. Plant biomass and its mercury content were determined 35 days after sowing.Only FS501 was able to grow on tailing containing 26.94ppm of mercury. Inoculation of the sweet sorghumwith the rhizobacteria does not aff ect its biomass but increase its mercury uptake signifi cantly. Mixing thetailing material with uncontaminated soil causes an increase in biomass but reduce the mercury content inplant signifi cantly. As a result, the amounts of removable mercury decrease signifi cantly. Inoculation withthe rhizobacteria has no eff ect on mixed material

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