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INDONESIA
Journal of Agricultural Science and Biotechnology
Published by Universitas Udayana
ISSN : 23020113     EISSN : -     DOI : -
Core Subject : Agriculture,
Mempublikasikan hasil-hasil penelitian yang berbasis bioteknologi dalam pemanfaatannya dibidang pertanian, termasuk biological control, pascapanen, serta semua penelitian tentang pemanfaatan komponen biologi yang dikemas dalam teknologi dalam lingkup pertanian secara luas.
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Articles 5 Documents
Search results for , issue "Vol 8 No 1 (2019)" : 5 Documents clear
Kelimpahan Populasi dan Persentase Serangan Lalat Buah (Bactrocera Spp.) (Diptera: Tephritidae) pada Tanaman Mentimun (Cucumis Sativus L.) di Beberapa Kabupaten Provinsi Bali Ni Wayan Suryati Agustini; A.A. Ayu Agung Sri Sunari; Ketut Ayu Yuliadhi
Journal of Agricultural Science and Biotechnology Vol 8 No 1 (2019)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

The research aims to determine the population abundance and the damage percentage of fruit fly. The study was conducted in Tabanan Regency villages ofCandikuning and Baturiti, Badung Regency villages of Belok-sidan and Kapal and Klungkung Regency villages of Gelgel which took place from February to April2019. Fruit sampling was done purposively by taking fruits that showed symptoms of fruit fly attacks which were then maintained in the Integrated Pest and Disease laboratory of the Agriculture Faculty, Udayana University. The identification results show that there were 2 species of fruit flies that attacked the cucumber, namely Bactrocera cucurbitae and Bactrocera calumniata. The population abundance of B. cucurbitae species in Tabanan Regency (69.7%), Badung Regency (71.2%) and Klungkung Regency (74%), while the population abundance of B. calumniata species in Tabanan Regency (30.2%), Badung Regency (28.8%) and Klungkung Regency (26%). The percentage of damage were in Tabanan Regency (22.9%), Badung Regency (22%) and Klungkung Regency (27.3%).
UJI ANTAGONIS BAKTERI ENDOFIT TERHADAP Colletotrichum scovillei PENYEBAB PENYAKIT ANTRAKSONA PADA CABAI BESAR (Capsicum annuum L.) I Gede Karta Satria Wibawa; Dewa Ngurah Suprapta; Khamdan Khalimi
Journal of Agricultural Science and Biotechnology Vol 8 No 1 (2019)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Colletotrichum scovillei is a newly discovered species causing anthracnose disease on chilli pepper (Capsicum annuum L.) in Bali. With the increasing reports of Colletotricum spp. resistance against chemical fungicide, biological control is taken as the alternatives to control this disease. In this study a total of 69 endophytic bacteria isolates was recovered from different solanaceae plants samples collected from nine regions of Bali and screened for the antagonism againstC. scovillei. From the potential 15 candidates of isolates with inhibition activity above 90%, hypersensitivity test were taken on tobacco leaf, and isolate CBCKB1 was chosen for further test. The CBCKB1 isolate was identified as Bacillus amyloliquefaciens based on molecular identification. Detached fruit bioassay proved that isolate CBCKB1 formula was effective in reducing the disease percentage and disease severity of anthracnose disease on chilli pepper.
TEKNIK STERILISASI DAN EFEKTIVITAS 2,4-D TERHADAP PEMBENTUKAN KALUS EKSPLAN DAUN NILAM (Pogostemon cablin Benth) IN VITRO Yuyun Fitriani; Gede Wijana; Ida Ayu Putri Darmawati
Journal of Agricultural Science and Biotechnology Vol 8 No 1 (2019)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Patchouli plant (Pogostemon cablin Benth) is a plant that produce essential oil which is called patchouli oil. Indonesia is the largest supplier of patchouli oil in the world market, which contribute about 90%. Advantage of patchouli oil in the perfume industry is a fixative property, it has the ability to bind with other oils therefore the scent can last for long time and has yet to be made synthetically. However, it takes very long time to produce essential oils in the conventional production system, becouse the growth of patchouli plant is relative slow. One alternative to produce the raw material or patchouli plant faster is through the tissue culture. The sterilization technique and the growth regulator are main factor in tissue culture. Therefore the purpose of this study was to find a sterilization technique, as wel as the concentration of growth regulators 2,4-D (2,4diclorophenoxy acetic acid) in MS + coconut water medium. Sterilization with HgCl2 mercury chloride) at concentration 0.1% for 10 min was the best method of sterilization to minimize contamination. Plant growth regulators 2,4-D at concentration 0.5 ppm was found very affectivein accelerating the formation of patchouli leaf callus in vitro. The callus formation occured retatively fast (9.67 days) with cream-colour, crumb texture, and with diameter 1.21 cm. The fresh and dry weight of callus were 1.35 g and 0.76 g respectively.
UJI EFEKTIVITAS RIZOBAKTERI SEBAGAIA GEN ANATGONIS Sclerotium rolfsii PENYEBAB PENYAKIT BUSUK BATANG PADA TANAMAN KACANG TANAH (Arachis hypogea L.) Ni Kadek Desy Andya Dewi; Dewa Ngurah Suprapta; Khamdan Khalimi
Journal of Agricultural Science and Biotechnology Vol 8 No 1 (2019)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Groundnut (Arachis hypogea L.) is one of important commodities in Indonesia, however the production of groundnut is still relatively low which ispartly because of the plant disease i.e. stem rot disease caused by Sclerotium rolfsii. Rhizobacteria is one of the solutions to be used as the biological controlagent against S. rolfsii. The aim of this study is to know the effectiveness of rhizobacteria to control stem rot disease caused by S. rolfsii. The results the studyshowed that among 130 isolates of rhizobacteria tested against S. rolfsii, 11 isolates showed inhibitory activity, and among them one isolate, BjTk11 fromexposed the highest inhibitory activity (96.09%) against S. rolfsii. On a green house experiment, treatment with formula of isolate BjTk 11 at doses of 5 g, 10 g,15 g and 20 g per polybag effectively controlled the pre emergence damping off (disease incidence = 0%) while the disease incidence on control was 23.5%. Nopost emergence damping off symptom was observed on groundnut treatment with formula of isolate BjTk11, while the disease incidence on control was 75%. The number of sclerotia in soil of control was 79/gram of soil which is obviously higher than those of treatments. The mode of action the rhizobacteria isolatesBjTk11 in inhibiting the growth of S. rolfsii is through antibiosis where the rhizobacteria produce the antifungal compound Butanedial, R - (-) - 1,2propanediol, 2,3-Butanediol, Isopropyl Alcohol, Acetic acid, anhydride with formic Acid , 2-bromohexane, 4,5-Diamino-6-hydroxypyrimidine, 4H-Pyran-4one, 3-hydroxy-2-methyl and Cyclotrisiloxane, hexamethyl-. The presence of these compounds probably closely related to the inhibitory activity against S. rolfsii.
Pemanfaatan Rizobakteri Penghasil Indole Acetic Acid Sebagai Agens Penginduksi Ketahanan Tanaman Tembakau Terhadap Cucumber Mosaic Virus (CMV) Ni Luh Gede Sriyanti; Khamdan Khalimi; I Gede Rai Maya Temaja
Journal of Agricultural Science and Biotechnology Vol 8 No 1 (2019)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Tobacco is a high economic value commodity, in order to meet the demand for tobacco in the market it requires good quantity and quality. One of the diseasethat often causes harm to tobacco is Cucumber Mosaic Virus (CMV). The utilization of indole acetic acid (IAA)-producing rhizobacteria can be used to increase plant growth and resistance from pathogenic infection. The aim of this study was to determine the effectiveness of IAA producing rhizobacteria formula in increasing the growth of tobacco plants in greenhouses, and to determine the effectiveness of rhizobacterial formulations in increasing tobacco plant resistanceagainst the infection of CMV. Identification of compounds in tobacco leaves that infected with CMV was carried out to determine the effectiveness of rhizobacteria in inducing plant resistance. Compound analysis was carried out using Gas Mass Chromatography Spectroscopy (GCMS). The effectiveness of rhizobacteria in increasing plant growth and resistance was conducted in a greenhouse. Based on the results of the study, four of IAA-producing rhizobacteria isolates were Stenotrophomonas maltophilia Sg3, Proteus mirabilis BjB17, Bacillus thuringiensis TNJbx3.3, and Bacillus cereus GR12, all the rhizobacterial isolates were effective in increasing plant height, leaf number, leaf chlorophyll content, wet weight and dry weight leaf. Based on the results of the rhizobacterial effectiveness test, all rhizobacterial isolates were effective in increasing plant resistance as indicated by a lower intensity of infection. Based on the analysis of the tobacco leaves compounds content showed that rhizobacteria S. maltophilia Sg3 and P. mirabilis BjB17 were able to induce resistance to tobacco plants by forming antiviral compounds namely 2-Naphthalene-sulfonic acid and rhizobacteria Bacillus cereus GR12 can stimulate the formation of 1-Naphthalene-sulfonic acid compounds and 2-Naphthalenesulfonic acidin tobacco as a form of induction of resistance to CMV infection.

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