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All Journal International Journal of Biosciences and Biotechnology Journal of Agricultural Science and Biotechnology Journal of Tropical Biodiversity and Biotechnology Caraka Tani: Journal of Sustainable Agriculture Jurnal Ilmu Administrasi Negara (JUAN) JCES (Journal of Character Education Society) Jurnal Analisis Sosial Politik JCRS (Journal of Community Research and Service) Arus Jurnal Sosial dan Humaniora jurnal administrasi politik dan sosial KOLONI Jurnal Pengabdian Sosial
Yuyun Fitriani
Program Studi Agronomi, Jurusan Budidaya Pertanian, Fakultas Pertanian, Universitas Udayana
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Public Health Social Sciences Other

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Efektivitas Program Asistensi Sosial Penyandang Disabilitas Berat (ASPDB) di Kota Bandar Lampung Fitriani, Yuyun
Jurnal Analisis Sosial Politik Vol 4 No 2 (2020): Jurnal Analisis Sosial Politik
Publisher : Fakultas Ilmu Sosial dan Ilmu Politik Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jasp.v4i2.60

Abstract

Abstract The Social Assistance Program for Persons with Severe Disabilities (ASPDB) is a form of state responsibility in fulfilling the rights of persons with disabilities and the embodiment of government social protection for its citizens. The target group of the ASPD program are people with severe disabilities who are unable to carry out activities to fulfill their daily lives so they need help from others. Measurement of effectiveness criteria is based on three main objectives of implementing the ASPDB program, namely: First, the fulfillment of the basic needs of life and daily care for persons with severe disabilities (clothing, food, clean water, daily care) so that their welfare level can be met fairly. Second, the growing concern of families and communities in providing social care and guidance for persons with severe disabilities. Third, growing efforts to fulfill the rights of persons with severe disabilities. Broadly speaking, the effectiveness of the implementation of the ASPDB program in Bandar Lampung has been well implemented. Families of persons with disabilities, which are the main milestones for the growth and development of persons with disabilities, have skills in caring for and looking after persons with disabilities. The community in the neighborhood where persons with disabilities live also do not discriminate against the existence of persons with disabilities so that persons with disabilities can play and interact with the community. Families of persons with disabilities, which are the main milestones for the growth and development of persons with disabilities, have skills in caring for and looking after persons with disabilities. The community in the neighborhood where persons with disabilities live also do not discriminate against the existence of persons with disabilities so that persons with disabilities can play and interact with the community. Families of persons with disabilities, which are the main milestones for the growth and development of persons with disabilities, have skills in caring for and looking after persons with disabilities. The community in the neighborhood where persons with disabilities live also do not discriminate against the existence of persons with disabilities so that persons with disabilities can play and interact with the community.
RUNNER-TIP CULTURE OF STRAWBERRY (Fragaria x ananassa Duch) GROWN ON SEVERAL SHOOT-INDUCTION MEDIUM Rindang Dwiyani; Hestin Yuswanti; Ni Nyoman Ari Mayadewi; Yuyun Fitriani
International Journal of Biosciences and Biotechnology Vol 8 No 1 (2020): Vol. 8 No. 1, September 2020
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2020.v08.i01.p02

Abstract

A research regarding “Runner-tip culture of strawberry (Fragaria x ananassa Duch) Grown on Several Shoot-induction Medium” has been investigated. The objective of the research was to find out the most suitable medium for shoot production from runner-tip culture of strawberry at establishment step of micropropagation. The research was laid out in a Completely Randomized Design, 4 treatments of medium type for shoot induction and 10 replication, each was represented by one (1) bottle with 6-8 explants. The treatments were summarized as follows: T1 = MS ; T2 = MS + 2 ppm BAP + 0.01 ppm NAA; T3 = MS + 1 ppm of TDZ; T4 = WPM + 2 ppm BAP + 0.01 ppm NAA. The parameters observed were days of the bud emergence, the average number of shoots per explant, and the average number of leaves per explant. It can be concluded that among medium used in the current research, the medium of MS added with 1 ppm thidiazuron (TDZ) was the most suitable medium for shoot production of strawberry from the explant of runner-tips. The treatment was resulted in the earliest time of bud emergence, and producing the highest number of shoots and leaves.
IN-PLANTA TRANSFORMATION METHOD MEDIATED WITH Agrobacterium tumefaciens FOR T-DNA TRANSFER IN TABLE GRAPE (Vitis vinifera L.) Rindang Dwiyani; Hestin Yuswanti; Yuyun Fitriani; Bambang Sugiharto
International Journal of Biosciences and Biotechnology Vol 6 No 2 (2019)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (502.538 KB) | DOI: 10.24843/IJBB.2019.v06.i02.p02

Abstract

The aim of the research is to investigate a simple method of in planta transformation method for T-DNA transfer in table grape. The T-DNA harbored the S0SPS1 gene under the control of promoter of the 35S CaMV from the Cauliflower Mosaic Virus and contained the NPTII gene, a kanamycin-resistant gene as a selectable marker for transformant selection. Six-month plants originated from cuttings were used as target plants. We explored two methods of in planta transformation, namely ”dipping” and “sweeping”. For both methods, the leaves of the target plants were removed and those of shoots without leaves were used as the target of transformation. In the “dipping method”, those shoots were dipped with the agrobacterial suspension for 60 seconds. However, for the “sweeping method”, the scars (the spots where leaves were removed) were swept with agrobacterial suspension using a cotton bud. Those treated non-leafy-shoots (from both methods) then were grown to be leafy shoots. Those leafy shoots then were cut and transplanted into the soil and grown to be a whole plant. The leaves of those plants then were taken as samples for DNA extraction and PCR using primers of NPTII gene (Forward: 5’-GTCATCTCACCTTCCTCCTGCC-3’; Reverse: 5’ GTCGCTTGGTCGGTCATTTCG-3’) with expected amplified band of 550 bp. We found that only the “sweeping method” plants amplified the 550 bp bands, while those of the “dipping method” did not. We suggest that the T-DNA was successfully integrated into the genome of plants treated with the “sweeping method” but not with the “dipping method”. Leaf sugar content (oBrix) of PCR-positive vines was higher than those of the wild-type vines, ensuring the integration of the T-DNA into the plant genome.
TEKNIK STERILISASI DAN EFEKTIVITAS 2,4-D TERHADAP PEMBENTUKAN KALUS EKSPLAN DAUN NILAM (Pogostemon cablin Benth) IN VITRO Yuyun Fitriani; Gede Wijana; Ida Ayu Putri Darmawati
Journal of Agricultural Science and Biotechnology Vol 8 No 1 (2019)
Publisher : Journal of Agricultural Science and Biotechnology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (531.11 KB)

Abstract

Patchouli plant (Pogostemon cablin Benth) is a plant that produce essential oil which is called patchouli oil. Indonesia is the largest supplier of patchouli oil in the world market, which contribute about 90%. Advantage of patchouli oil in the perfume industry is a fixative property, it has the ability to bind with other oils therefore the scent can last for long time and has yet to be made synthetically. However, it takes very long time to produce essential oils in the conventional production system, becouse the growth of patchouli plant is relative slow. One alternative to produce the raw material or patchouli plant faster is through the tissue culture. The sterilization technique and the growth regulator are main factor in tissue culture. Therefore the purpose of this study was to find a sterilization technique, as wel as the concentration of growth regulators 2,4-D (2,4diclorophenoxy acetic acid) in MS + coconut water medium. Sterilization with HgCl2 mercury chloride) at concentration 0.1% for 10 min was the best method of sterilization to minimize contamination. Plant growth regulators 2,4-D at concentration 0.5 ppm was found very affectivein accelerating the formation of patchouli leaf callus in vitro. The callus formation occured retatively fast (9.67 days) with cream-colour, crumb texture, and with diameter 1.21 cm. The fresh and dry weight of callus were 1.35 g and 0.76 g respectively.
BIOEKOLOGI Grapevine leafroll-Associated Virus type 3 (GLRAV-3) PENYEBAB PENYAKIT DAUN MENGGULUNG PADA TANAMAN ANGGUR (Vitis vinifera L.) Yuyun Fitriani; I Gede Rai Maya Temaja; G. N. Alit Susanta Wirya
Journal of Agricultural Science and Biotechnology Vol 7 No 1 (2018)
Publisher : Journal of Agricultural Science and Biotechnology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (387.162 KB)

Abstract

The survey of vineyard fields in Buleleng, Bali, Indonesia was conducted, some grapevine cultivars were found had grapevine leaf roll disease symptomps. The symptoms including interveinal reddening or yelowing and fall leaves, down rolling of leaves, phloem disruption, and the fruit maturation is delayed. The analysis of enzyme linked immunosorbent assay (ELISA) of symptom leaf with Grapevine leaf roll-associated virus type 3 (GLRaV-3) antiserum shown the disease was corresponding to the GLRaV-3. The study was conducted to identify the mealybug that colonized of grapevine, to determine capability of mealybug to transmitted GLRaV-3 and host range GLRaV-3.Base on the survey ofmealybuginvineyard fields in Buleleng, Bali, the two types of mealybug were identified, namely Ferrisia virgata (Ckll.) (Hemiptera: Pseudococcidae) and Maconellicoccus hirsutus (Green) (Hemiptera: Pseudococcidae). Results of the transmission test showed that only M. Hirsutus are capable of transmitting GLRaV-3. The plant range host test of GLRaV-3 inoculation with M. hirsutuswas conducted and the symptom was detected on3plants species, grapevines (Vitis vinifera), native gooseberry (Physalis floridana) andshoe flower (Hibiscus rosa-sinensis) from18 species of plants that used.
The Alternative Media Supporting the Protocorm and Plantlet Growth of the Indonesian Black Orchid (Coelogyne pandurata Lindl.) Grown In Vitro Rindang Dwiyani; Yuyun Fitriani; Ixora Sartika Mercuriani
Caraka Tani: Journal of Sustainable Agriculture Vol 37, No 1 (2022): April
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v37i1.55956

Abstract

Due to the high cost of the most often used basic media in tissue culture, it is vital to identify more affordable alternatives. This research aimed to determine the best alternative culture media for the protocorm and plantlet growth of Coelogyne pandurata Lindl. It employed a completely randomized design, eight treatments and four replications. The treatments referred to the different types media and consisted of M1 = New Phalaenopsis (NP) medium, M2 = a medium made from foliar fertilizer (FFM), M3 = NP + 2 cc L-1 AB mix solution (a media for hydroponics), M4 = FFM + 2 cc L-1 AB mix solution, M5 = NP + 50 cc L-1 of tomato extract, M6 = NP + 50 cc L-1 of bean sprout extract, M7 = FFM + 50 cc L-1 of tomato extract and M8 = FFM + 50 cc L-1 of bean sprout extract. The M4 medium exhibited the best results in terms of average leaves count (4.80), average shoot length (2.68 cm), average root length (4.35 cm), the average fresh weight per plantlet (214.5 mg) and dry weight of plantlets (73.1 mg). The average number of roots per plantlet was 4.25, acquired using the less expensive M8 treatment, which also produces a negligible number of leaves (4.50). In conclusion, the M4 medium is the most appropriate medium for growing protocorm and plantlet of C. pandurata. The experiment also found that the FFM basic medium combined with 50 cc L-1 of bean sprout extract can be used as another cheaper alternative for growing protocorms of C. pandurata.
SMART VILLAGE AS A FORM OF SUSTAINABLE VILLAGE DEVELOPMENT IN LAMPUNG PROVINCE Fitriani, Yuyun
Journal of Community Research and Service Vol 8, No 1: January 2024
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jcrs.v8i1.53713

Abstract

The current smart village concept is an activity to optimize village government services by increasing the use of information technology to support the progress of village communities in Lampung Province . The purpose of writing this article is to provide an overview of smart village development as a form of sustainable development in Lampung Province. The method used in this research activity is a qualitative method with a descriptive approach. The research results show that the implementation of smart villages in Lampung Province has gone well. Based on the three main elements of a smart village, namely smart government, smart community, and smart environment shows that these three elements can influence each other and can be a determining factor in the quality of public services in the village
Intraspecific SSR Marker Screening for Detection of Dendrobium crumenatum Mutants Generated from In Vitro Gamma Irradiation Dwiyani, Rindang; Sanjaya, I Putu Wahyu; Yuswanti, Hestin; Darmawati, Ida Ayu Putri; Suada, I Ketut; Manullang, Prila Kartika; Fitriani, Yuyun
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 2 (2024): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.89896

Abstract

Determination of D. crumenatum mutant obtained from in vitro mutation breeding needs a long time due to its long-life cycle. SSR molecular markers can be used for early mutant detection. Specific SSR markers developed in D. crumenatum are not yet available. Alternative published SSR markers were developed from D. catenatum. The aims of this study are to screen the most informative SSR markers generated from D. catenatum tested in irradiated D. crumenatum population and to determine the gamma irradiation dose resulting the most mutants. Ten SSR markers were randomly selected and tested in 25 individuals of D. crumenatum plantlets irradiated with several doses (0, 5, 10, 15, and 20 Gy; 5 plantlets each dose). The result showed 7 of 10 primers were polymorphic and the other three were  monomorphic. All seven polymorphic primers can be used to identified intraspecific variation in the D. crumenatum mutant population. Markers dnsr28 and dnsr98 were the most informative, with the highest polymorphic information content (PIC) value of 0.5. Irradiation D. crumenatum protocorms using 10-15 gray doses were detected as the highest mutant percentage obtained up to 100% in the sample tested. This resulting marker information can be used to screen wider mutant population to decrease the non-mutant individuals in the population for maintenance and cost efficiency. The 10-15 Gy can be used as reference doses for gamma irradiation in 3 months old D. crumenatum protocorm materials. 
Meristem Culture of Strawberry Plants (Fragaria x Ananassa Duch.) Sachinoka Variety on Various Types of Basic Media In Vitro Fitriani, Yuyun; Yuswanti, Hestin; Dwiyani, Rindang; Darmawati, Ida Ayu Putri
International Journal of Biosciences and Biotechnology Vol 11 No 1 (2023): International Journal of Biosciences and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2023.v11.i01.p08

Abstract

The large number of in vitro media circulating in the market is a challenge to find the most effective media for sachinoka strawberry meristem culture, because the need for in vitro media depends on genetic intelligence. The purpose of this study was to find the best media from the various tested media to reproduce the Sachinoka variety strawberry seedlings in a relatively short time using the meristem culture method. This study used a completely randomized design (CRD) with 6 media treatments and repeated 7 times, namely: M1 (WPM without BA), M2 (WPM + 4 ppm BA), M3 (NP without BA), M3 (NP + 4 ppm BA), M5 (BMM without BA), M6 (BMM + 4 ppm BA). The variables observed were the time of emergence of shoots, the number of shoots and the number of leaves. The results of this study indicate that there is no significant difference for the emergence of shoots variable. Treatment M1 produced the highest number of shoots (12.29) but not significantly different from M5 (10.14), both were significantly different from both treatments. The highest number of leaves is in M1 (27.14). It can be concluded that the best medium for the growth of sachinoka strawberry meristem culture is WPM media with the addition of 4 ppm BA, while the second-best medium is BMM media with the addition of 4 ppm BA.
Peningkatan Partisipasi Perempuan Dalam Bidang Sosial, Politik, Hukum Dan Ekonomi Fitriani, Yuyun; Utoyo, Bambang; Saputri, Maysa Yoga; Putri, Agustina Jesica; Aprilianti, Ni Wayan Sri; Handayani, Elvita; S, Umi Fatimah; Saputri, Ayu; Mayasari, Desi
Jurnal Pengabdian Sosial Vol. 1 No. 10 (2024): Agustus
Publisher : PT. Amirul Bangun Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59837/dqpjgw03

Abstract

Masih rendahnya partisipasi perempuan dalam bidang sosial, politik, hukum dan ekonomi di pedesaan menjadi tolok ukur pelaksanaan kegiatan pengabdian ini. Perempuan merupakan salah satu subjek yang rentan terdiskriminasi posisinya baik di ranah publik maupun domestik. Dominasi budaya patriarkhi yang telah mengakar di sebagian masyarakat Indonesia menjadi penyebab ketimpangan peran laki-laki dan perempuan di berbagai aspek kehidupan baik sosial, politik, ekonomi, maupun budaya.  Kegiatan pengabdian ini dilaksanakan dengan tujuan untuk meningkatkan pemahaman dan partisipasi perempuan dalam bidang sosial, politik, hukum dan ekonomi agar perempuan tidak terbelenggu dalam paradigm lama yang menganggap perempuan tidak mampu berdaya. Metode yang digunakan dalam pengabdian ini dimulai dengan focus group discussion dan dilanjutkan dengan kegiatan sosialisasi. Pasca pelaksanaan pengabdian pemahaman perempuan Desa Purwo Adi, Kecamatan Trimurjo, Kabupaten Lampung Timur semkin meningkat. Hal tersebut dapat dilihat dengan peningkatan hasil post test peserta kegiatan.
Co-Authors Akbar, Andini Anzani, Risa Dwi Aprilianti, Ni Wayan Sri Ayu Saputri, Ayu Bambang Sugiharto Bambang Utoyo Desi Mayasari, Desi Dewanti, Asri Aprilla Eka Putri, Ananda Desti Fujiaturrahman, Sukron GEDE WIJANA Gusti Ngurah Alit Susanta Wirya Handayani, Elvita HESTIN YUSWANTI Husni, Daud I G. R. M. TEMAJA I Ketut Suada I Made Sudana I NYOMAN GEDE ASTAWA I NYOMAN RAI I Wayan Lendra Ida Ayu Putri Darmawati Ixora Sartika Mercuriani Manullang, Prila Kartika Mirnawati Mirnawati Musliyono, Musliyono Nadira, Nadira NI NYOMAN ARI MAYADEWI Nurnadya, Nurnadya Putri, Agustina Jesica Rindang Dwiyani S, Umi Fatimah Sanjaya, I Putu Wahyu Saputri, Maysa Yoga Sukmawati Sukmawati