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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 14 Documents
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Search results for , issue " Vol 11, No 1 (2012)" : 14 Documents clear
SIFAT ANTIOKSIDAN, KANDUNGAN FENOLAT TOTAL dan FLAVONOID TOTAL EKSTRAK KULIT BATANG MERTAPANG (Terminalia copelandii Elmer) [Antioxidant Properties, Total Phenolic and Total Flavonoid Content of Mertapang (Terminalia copelandii Elmer) Bark Extract] Murningsih, Tri
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (369.622 KB) | DOI: 10.14203/beritabiologi.v11i1.1884

Abstract

The antioxidant properties of methanolic extract of T. copelandii Elmer bark have been evaluated by using free radical (DPPH) scavenging assay, reduction power and ß-caroten bleaching assay. Ascorbic acid (vitamin C) and “butylated hydroxytoluene” (BHT) were used as positive control or antioxidant standard. Total phenolic content was estimated by using Folin-Ciocalteu reagent and expressed in gallic acid (Gallic Acid Equivalent), while total flavonoid content was estimated by using aluminium chloride reagent and expressed in routine (Routine Equivalent). The result shows antiradical activity of extract on concentration 40 µg/ml was 89.96%, lower than vitamin C (95.61%) but higher than BHT ((52.25%). The reducing power of extract on concentration 50 µg/ml has absorbance value 0.644 lower than vitamin C (0.769), but higher than BHT (0.475). During 2 hours incubation, extract on concentration 50 µg/ml inhibited ß-caroten bleaching 69.46% higher than control negative (35.81%), but lower than BHT (85.99%). Estimation of total phenolic and total flavonoid content showed that extract contained 350.40 µg (GAE)/g extract and 277.50 µg (RE)/g extract respectively. The antioxidant properties may be attributed to the presence of phenolic and flavonoid compounds present in the extract.
TINJAUAN TENTANG KOPEPODA PARASIT DI INDONESIA [A Review of Parasitic Copepods in Indonesia] Sidabalok, Conni
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (277.734 KB) | DOI: 10.14203/beritabiologi.v11i1.456

Abstract

Parasitic copepods are of the most important parasites in the marine environment. They occupy a wide range of hosts ranging from many major groups of invertebrates to the top predators in the sea. Indonesia with vast ocean area has great opportunity to harbour a large numbers of parasitic copepods. However, only few studies of parasitic copepods have been conducted in this region, and most of them are preliminary studies, particularly on mariculture associated copepods. To date, only 70 species of parasitic copepods have been recorded from Indonesian waters. There is a lack of taxonomical approach on these studies, in which some of the specimens were not identified to species level.This highlights the need for taxonomic study and even molecular study of marine parasitic copepods in Indonesia. This paper aims to review the evolution of research on parasitic copepods in Indonesia and to identify areas in need of future research.
CEKAMAN OKSIDASI SEL KHAMIR Candida tropicalis YANG DIPERLAKUKAN DENGAN PARASETAMOL DAN ANTIOKSIDAN (+)-KATEKHIN [Oxidative Stress in Candida tropicalis Treated with Paracetamol and Antioxidant (+)-catechin] Julistiono, Heddy
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i1.1889

Abstract

In order to more understand similarity of yeast Candida tropicalis with mammalian cells in analgesic drug paracetamol metabolism and toxicity, ability of yeast in the drug metabolism and oxidative response of cells treated with the drug and (+)-catechin was studied. In mammalian cells, paracetamol toxicity is mainly caused by metabolite byproduct of drug metabolism catalyzed by cytochrome P450, a membrane-bound enzyme and peroxidase and a soluble enzyme. Previously it has been shown that paracetamol induced oxidative stress in the yeast cells; and green tea extract protected the cells from oxidation. In this study, it had been shown that paracetamol could be metabolized by yeast cell suspension or cell free extracellular protein, reflecting possibility of role of enzyme that can not be separated from cell and that is soluble, which is common phenomenon in mammalian cell system. Paracetamol of 3 mg/ml increased lipid peroxidation, a marker of oxidative stress. A green tea polyphenol, (+)-catechin of 0.1 mg/ml did not decrease lipid peroxidation content induced by paracetamol. At higher concentration (2 mg/ml), solely (+)-catechin did not increase lipid peroxidation content. Paracetamol or (+)-catechin induced slightly activity of superoxide dismutase enzyme. The data indicated that paracetamol metabolism or toxicity in the yeast may be similar to that of mammalian cells. In this condition, it suggested that (+)-catechin is one of polyphenol green tea that has weak activity of antioxidant and consequently has weak activity of prooxidant. Mechanism of paracetamol toxicity in C. tropicalis is still to be studied with emphasis on the free radical formation and drug metabolism.
KOI HERPES VIRUS SEBAGAI PENYEBAB KEMATIAN MASSAL Cyprinus carpio koi DI INDONESIA [Koi Herpes Virus the Causative Agent of Sporadically Mortality of Cyprinus carpio koi in Indonesia] Madyowati, Sri Oetami; Sumaryam, Sumaryam; Kusyairi, A; Suprapto, Hari
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (200.538 KB) | DOI: 10.14203/beritabiologi.v11i1.1878

Abstract

Virus was isolated from infected koi Cyprinus carpio koi with KHV and confirmed by polymerase chain reaction (PCR) and transmission electron microscope (TEM). Fish that were naturally living near koi pond such as catfish Clarias batrachus, nila Oreochromis niloticus, koki Carrasius auratus, and komet Macropinna microstoma did not infected after the cohabitation test. Based on the results KHV was the agent responsible for mortality of million cultured koi in East Java Province Indonesia. Absolute mortality was occurred in koi between 48-72 h post infection, while other cyprinids family was not caused in mortality. Other fresh water fish such as catfish Clarias batrachus, nila Oreochromis niloticus, koki Carrasius auratus, and komet Macropinna microstoma was not infected. Virus-like particles as found in gill ofinfected fish with KHV.

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