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BERITA BIOLOGI

berita_biologi
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Published by Lembaga Ilmu Pengetahuan Indonesia

ISSN : - EISSN : - DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 30 Documents

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Search results for , issue "Vol 11, No 1 (2012)" : 30 Documents clear

SIFAT ANTIOKSIDAN, KANDUNGAN FENOLAT TOTAL dan FLAVONOID TOTAL EKSTRAK KULIT BATANG MERTAPANG (Terminalia copelandii Elmer) [Antioxidant Properties, Total Phenolic and Total Flavonoid Content of Mertapang (Terminalia copelandii Elmer) Bark Extract] Murningsih, Tri
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The antioxidant properties of methanolic extract of T. copelandii Elmer bark have been evaluated by using free radical (DPPH) scavenging assay, reduction power and Ã-caroten bleaching assay. Ascorbic acid (vitamin C) and âbutylated hydroxytolueneâ (BHT) were used as positive control or antioxidant standard. Total phenolic content was estimated by using Folin-Ciocalteu reagent and expressed in gallic acid (Gallic Acid Equivalent), while total flavonoid content was estimated by using aluminium chloride reagent and expressed in routine (Routine Equivalent). The result shows antiradical activity of extract on concentration 40 Âµg/ml was 89.96%, lower than vitamin C (95.61%) but higher than BHT ((52.25%). The reducing power of extract on concentration 50 Âµg/ml has absorbance value 0.644 lower than vitamin C (0.769), but higher than BHT (0.475). During 2 hours incubation, extract on concentration 50 Âµg/ml inhibited Ã-caroten bleaching 69.46% higher than control negative (35.81%), but lower than BHT (85.99%). Estimation of total phenolic and total flavonoid content showed that extract contained 350.40 Âµg (GAE)/g extract and 277.50 Âµg (RE)/g extract respectively. The antioxidant properties may be attributed to the presence of phenolic and flavonoid compounds present in the extract.

TINJAUAN TENTANG KOPEPODA PARASIT DI INDONESIA [A Review of Parasitic Copepods in Indonesia] Sidabalok, Conni
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Parasitic copepods are of the most important parasites in the marine environment. They occupy a wide range of hosts ranging from many major groups of invertebrates to the top predators in the sea. Indonesia with vast ocean area has great opportunity to harbour a large numbers of parasitic copepods. However, only few studies of parasitic copepods have been conducted in this region, and most of them are preliminary studies, particularly on mariculture associated copepods. To date, only 70 species of parasitic copepods have been recorded from Indonesian waters. There is a lack of taxonomical approach on these studies, in which some of the specimens were not identified to species level.This highlights the need for taxonomic study and even molecular study of marine parasitic copepods in Indonesia. This paper aims to review the evolution of research on parasitic copepods in Indonesia and to identify areas in need of future research.

CEKAMAN OKSIDASI SEL KHAMIR Candida tropicalis YANG DIPERLAKUKAN DENGAN PARASETAMOL DAN ANTIOKSIDAN (+)-KATEKHIN [Oxidative Stress in Candida tropicalis Treated with Paracetamol and Antioxidant (+)-catechin] Julistiono, Heddy
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i1.1889

In order to more understand similarity of yeast Candida tropicalis with mammalian cells in analgesic drug paracetamol metabolism and toxicity, ability of yeast in the drug metabolism and oxidative response of cells treated with the drug and (+)-catechin was studied. In mammalian cells, paracetamol toxicity is mainly caused by metabolite byproduct of drug metabolism catalyzed by cytochrome P450, a membrane-bound enzyme and peroxidase and a soluble enzyme. Previously it has been shown that paracetamol induced oxidative stress in the yeast cells; and green tea extract protected the cells from oxidation. In this study, it had been shown that paracetamol could be metabolized by yeast cell suspension or cell free extracellular protein, reflecting possibility of role of enzyme that can not be separated from cell and that is soluble, which is common phenomenon in mammalian cell system. Paracetamol of 3 mg/ml increased lipid peroxidation, a marker of oxidative stress. A green tea polyphenol, (+)-catechin of 0.1 mg/ml did not decrease lipid peroxidation content induced by paracetamol. At higher concentration (2 mg/ml), solely (+)-catechin did not increase lipid peroxidation content. Paracetamol or (+)-catechin induced slightly activity of superoxide dismutase enzyme. The data indicated that paracetamol metabolism or toxicity in the yeast may be similar to that of mammalian cells. In this condition, it suggested that (+)-catechin is one of polyphenol green tea that has weak activity of antioxidant and consequently has weak activity of prooxidant. Mechanism of paracetamol toxicity in C. tropicalis is still to be studied with emphasis on the free radical formation and drug metabolism.

KOI HERPES VIRUS SEBAGAI PENYEBAB KEMATIAN MASSAL Cyprinus carpio koi DI INDONESIA [Koi Herpes Virus the Causative Agent of Sporadically Mortality of Cyprinus carpio koi in Indonesia] Madyowati, Sri Oetami; Sumaryam, Sumaryam; Kusyairi, A; Suprapto, Hari
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Virus was isolated from infected koi Cyprinus carpio koi with KHV and confirmed by polymerase chain reaction (PCR) and transmission electron microscope (TEM). Fish that were naturally living near koi pond such as catfish Clarias batrachus, nila Oreochromis niloticus, koki Carrasius auratus, and komet Macropinna microstoma did not infected after the cohabitation test. Based on the results KHV was the agent responsible for mortality of million cultured koi in East Java Province Indonesia. Absolute mortality was occurred in koi between 48-72 h post infection, while other cyprinids family was not caused in mortality. Other fresh water fish such as catfish Clarias batrachus, nila Oreochromis niloticus, koki Carrasius auratus, and komet Macropinna microstoma was not infected. Virus-like particles as found in gill ofinfected fish with KHV.

ADAPTASI OSMOTIK TUMBUHAN MANGROVE AVICENNIA MARINA (FORSSKÃ¥L) * VIERH. DAN KEDELAI (GLYCINE MAX (L.) MERR.) TERHADAP STRES SALINE Naiola, Beth Paul
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i1.480

The extent of salinity effect land due to bad agricultural practices and global climate change that could affect the productivity of cultivated plants in agricultural and coastal areas as a result of rising sea levels, encourage the study of cultivated plants resistance to salinity stress. This research is aimed to observe the mangrove Avicennia marina (Forsskål) Vierh., a halophyt, grew up on the beach as a plant model that is able to adapt to saline environment by pumping out the NaCl (sodium pump) and deposited on the leaf surface. On the application of sea water with a multilevel concentrations (0, 25, 50 and 75%) A. marina is able to adjust by changing the osmotic potential in line with the level of concentration of sea water, as an indication of the course of osmotic regulation. Meanwhile, soybean var. Anjasmoro only able to adapt to sea water at a concentration of 25%, equivalent to 150 mM NaCl. This result may be the starting point by breeders and biotecknolo gists in utilizing the potential genes drives the salinity resistance characters in Indonesian mangrove species (especially Avicennia marina) to contrive salinity resistance cultivars of cultivated food plants with better yield.

IDENTIFIKASI ALEL GEN Xa7 PADA PLASMA NUTFAH PADI LOKAL PAREKALIGOLARA MELALUI UJI SEGREGASI FENOTIPE DAN GENOTIPE Dwinita W Utami; TS Kadir; A Nasution
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i1.457

Bacterial Leaves Blight (BLB) is one of the major diseases in rice. Among the disease resistance genes HDB, Xa7 gene still can compensate the diversity of Indonesian BLB pathogen in the field. One of the local rice germplasm, Parekaligolara (Indica, IRN: 1541) were selected based on allele mining research which contained a Xa7 alleles. The purpose of this study is to identify the alleles of the Xa7 gene contained in Indonesian local rice Parekaligolara germplasm in rice through the segregation testing based on phenotype and genotype performance of segregating population derived from the crossed with the susceptible control varieties, TN1. The segregation testing were conducted in F1 and F2 populations and analyzed on phenotypes of BLB resistance and genotypes using Xa7SNP markers. The analysis results showed that the Xa7 allele variation founded in the Parekaligolara germplasm are act as dominant alleles that contribute on the BLB resistance trait particularly to Race VIII of BLB pathogen. Xa7SNP8 is polymorphic markers for Xa7 alleles gene that are found in Parekaligolara. This marker could be used as a marker assisted for selection, if Parekaligolara's Xa7 alleles will use as a parent in the BLB disease resistant rice breeding program.

SEBARAN DAN HABITAT KUKANG JAWA (Nycticebus javanicus) DI AREA PERKEBUNAN SAYUR GUNUNG PAPANDAYAN, KABUPATEN GARUT Wirdateti Wirdateti
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i1.1887

The need of medicine product are increasing now. The fermentation of rice by using Monascus purpureus produce lovastatin, pigments and citrinin as secondary metabolites. The aim of this study was to know the concentration of lovastatin, red and yellow pigments and citrinin in rice which fermented by Monascus purpureus that had been mutated by using ultraviolet irradiation.Three strains were used in this research, such as AsK, yellow Jmba and red Jmba.The result were analyzed by using HPLC and Spectrofotometer. The result showed that the yellow Jmba strain which treated by UV mutagenesis for 3 minutes contain higher lovastatin (1.798%) and pigment and lower citrinin (0.019%) compare to without mutagenesis. On the other hand ASK strain produced higher lovastatin (1.903%) but higher citrinin (1.270%).This research is expected to provide scientific information to the public. That M. purpureus contained lovastatin that is beneficial, red and yellow pigments for natural food colorant.

POTENSI Enterolobium cyclocarpum (Willd.) Griseb DAN Centrosema pubescens Benth. SEBAGAI AKUMULATOR PENCEMAR MERKURI Nuril Hidayati
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i1.1883

Some plant species growing in the contaminated areas, indicated high tolerance and potentially affective in accumulating pollutants. These plants can be utilized as hyper accumulators for cleaning up contaminated sites. This research aims to examine plants that grow under Hg contaminated media and to analyze their potency as hyper accumulators. Two pioneer plant species Enterolobium cyclocarpum (Willd.) Griseb and Centrosema pubescens Bent. that proven tolerant and dominant at contaminated sites of gold mine were examined in this research. The plants were grown in liquid media added with mercury (HgCl2) with different level of concentrations 0 ppm, 10 ppm and 20 ppm. Chelate Ammonium Thiosulphate [(NH4)2S2O3] was added with concentration levels of 0 ppm and 20 ppm. Plant performances, cellular responses, Hg accumulation in the plant were assessed. The results revealed that the growth of both plant species decreased with the increase of Hg contamination level. The presents of chelating agent improved adaptability of the plants, indicated by the increase of biomass production and Hg content in the plants, even at the highest level of Hg concentration (20 ppm). Cellular responses showed at 20 ppm Hg, indicated by the decrease of both the number and the size of epidermis and pith cells. Accumulation of Hg in the plants increased with the increased of Hg concentration in the media. Mercury accumulation in both plant species were more concentrated in the root system rather than the shoot.

ADAPTASI OSMOTIK TUMBUHAN MANGROVE Avicennia marina (Forsskål) * Vierh. DAN KEDELAI (Glycine max (L.) Merr.) TERHADAP STRES SALINE Beth Paul Naiola
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i1.480

ANALISA KANDUNGAN LOVASTATIN, PIGMEN DAN CITRININ PADA FERMENTASI BERAS IR-42 DENGAN MUTAN Monascus purpureus N Nurhidayat
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i1.1888

The need of medicine product are increasing now.The fermentation of rice by using Monascus purpureus produce lovastatin, pigments and citrinin as secondary metabolites. The aim of this study was to know the concentration of lovastatin, red and yellow pigments and citrinin in rice which fermented by Monascus purpureus that had been mutated by using ultraviolet irradiation.Three strains were used in this research, such as AsK, yellow Jmba and red Jmba.The result were analyzed by using HPLC and Spectrofotometer.The result showed that the yellow Jmba strain which treated by UV mutagenesis for 3 minutes contain higher lovastatin (1.798%) and pigment and lower citrinin (0.019%) compare to without mutagenesis.On the other hand ASK strain produced higher lovastatin (1.903%) but higher citrinin (1.270%).This research is expected to provide scientific information to the public.That M. purpureus contained lovastatin that is beneficial,red and yellow pigments for natural food colorant.

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