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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 24 Documents
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Search results for , issue "Vol 15, No 2 (2016)" : 24 Documents clear
PENURUNAN KADAR TANIN DAN ASAM FITAT PADA TEPUNG SORGUM MELALUI FERMENTASI Rhizopus oligosporus, Lactobacillus plantarum dan Saccharomyces cerevisiae Raden Haryo Bimo Setiarto; Nunuk Widhyastuti
BERITA BIOLOGI Vol 15, No 2 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i2.2295

Abstract

Problems in using sorghum flour as food material was the presence of tannin and phytic acid that can reduce nutritition quality of sorghum flour. This study aimed to analyze the influence of Rhizopus oligosporus, Lactobacillus plantarum and Saccharomyces cerevisiae fermentation on the reduction levels of tannin and phytic acid in sorghum flour. Production of sorghum flour was done by four treatments in triplo i.e control (without fermentation), liquid fermentation (with L. plantarum and S. cerevisiae), solid fermentation (with R. oligosporus), mixture of solid and liquid fermentation (with R. oligosporus, L . plantarum and S. cerevisiae). Analysis levels of tannin and phytic acid in sorghum flour fermentation was performed by using spectrophotometry technique. The results showed that the fermentation process was able to reduce levels of tannin from 29.13 to 33.69% and phytic acid levels from 13.36% to 44.65% on sorghum flour. The highest reduction levels of tannin and phytic acid was produced in mixture of solid and liquid fermentation 33.69% and 44.65% respectively. Reduction levels of tannin and phytic acid can be caused of tannase and phytase enzyme which produced by the microbes during the fermentation processes.   
EVALUASI AKTIVITAS ANTI-INFLAMASI DAN ANTIOKSIDAN SECARA IN-VITRO, KANDUNGAN FENOLAT DAN FLAVONOID TOTAL PADA TERMINALIA SPP. Tri Murningsih; Ahmad Fathoni
BERITA BIOLOGI Vol 15, No 2 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i2.2264

Abstract

Terminalia is the second largest genus of Combretaceae family, consists of 200 species distributed in the tropics and subtropics. Many species of Terminalia are well kown in traditional medicine  in several countries of Africa and Asia. In this study, bark extracts of four species of Terminalia (T. catappa, T. citrina, T. bellirica and T. macadamii) were evaluated for their in-vitro anti-inflammatory and antioxidant activity. Determination of the anti-inflammatory activity was carried out using red blood cell membrane stabilization assay and the antioxidant activity was carried out using DPPH free radical scavenging and ferric reducing power assay. Estimation of total phenolic   content was carried out using Folin-Ciocalteu reagent, while  total flavonoid content using alumminium trichloride reagent. The results showed that extract of T. catappa has the most potent anti-inflammatory activity (IC50= 97,83±0,0100 ?g/mL) and the most powerful  antioxidant activity of DPPH free radical scavenging (IC50= 21,8900 ± 0,0264 ?g/mL) and ferric reducing power (IC50= 87,1533 ± 0,04163 ?g/ mL). The weakest anti-inflammatory activity was T. citrina extract (IC50= 159,35±0,0200 ?g/mL), whereas T. bellirica extract demonstrated the weakest antioxidant activity of  DPPH free radical scavenging (IC50= 44,4867 ± 0,0153 ?g/mL) and ferric reducing  power (IC50= 140,89±0,04 ?g/mL). The extract of T. citrina has the highest content of total phenolic (84,8167 ± 0,53407 mg GAE/g) and flavonoid (23,1200 ± 1,7149 mg RE/g) while the T. macadamii extract has the lowest content of total phenolic (24,3700 ± 0,0173 mg GAE/g) and flavonoid (13,2667 ± 0,3386 mg RE/g).
OPTIMASI PRODUKSI SERTA ANALISIS AKTIVITAS ANTIOKSIDAN DAN ANTIMIKROBA SENYAWA EKSOPOLISAKARIDA DARI JAMUR TIRAM PUTIH (Pleurotus ostreatus) PADA MEDIA CAIR Iwan Saskiawan; Misbahul Munir; Suminar S Achmadi
BERITA BIOLOGI Vol 15, No 2 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i2.2299

Abstract

Pleurotus ostreatus is well known as an oyster mushroom that is very popular because of its high nutritional value and pharmaceutical component. The aim of this research was to study the antioxidant and antibacterial activity of exopolysaccharides from P. ostreatus grown on liquid medium.The P. ostreatus was grown on Mushroom Complete Medium (MCM) containing various types of Carbon (C) and Nitrogen (N) sources, i.e glucose, lactose, amylose and sucrose as a Carbon sources and yeast extract, polypeptone, NH4Cl, and NH4NO3 as a Nitrogen sources. The results showed that sucrose and yeast extract were the best source of Carbon and Nitrogen that produced 208 and 100 mg/L of exopolysaccharides.  The exopolysaccharide exhibited antimicrobial activity against Bacillus subtilis and Escherichiacoli. However, it did not have antimicrobial activity against Saccharomyces cerevisiae.  In addition, the exopolysaccharide indicated to have an antioxidant activity.
IDENTIFIKASI GEN / QTL (Quantitative Trait Loci) SIFAT TOLERAN CEKAMAN ALUMINIUM PADA GALUR-GALUR PADI GOGO Dwinita Wikan Utami; I Rosdianti; S Yuriyah; AD Ambarwati; I Hanarida; Suwarno Suwarno; Miftahudin Miftahudin
BERITA BIOLOGI Vol 15, No 2 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i2.2276

Abstract

Program on the ability of agriculture to provide rice as a staple food tends to decrease with the reduced land area used for rice cultivation. improvement rice production should be directed to use  marginal land  as dry and acid land, where poisoning aluminium was seen as a constrain factor. Development of Aluminium (Al) tolerant variety is needed to increase rice production. This technology was environmentally friendly, cheaper and also easier for farmers adopted. The rice lines with broad genetics background which tolerant to Al toxicity has been produced by breeding program. The diversity of Indonesian local rice germplasm is a basic foundation on development of Al toxicity tolerant rice varieties. The purpose of this research was to analyze the genotype variety using 384 SNPs markers on selected Al tolerant rice lines based on bioinformatics approach. Based on the 384 SNPs markers, the total 26 selected  genotypes were clustered into 5 groups. Each group was having varied Relative Root Length (RRL), which was an Al tolerant parameter of phenotype. These groups were indicated for having different genes or QTL that were related to the Al tolerant character. The association analysis obtained 9 significant SNPs markers that can be applied as the molecular markers for assisting selection in breeding  program.

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