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All Journal Journal of Tropical Biodiversity and Biotechnology BERITA BIOLOGI Jurnal AGROSAINS dan TEKNOLOGI
Iwan Saskiawan
Research Center for Biology, Indonesian Institute of Sciences
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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Antifungal Activity of Bacterial Isolates from Straw Mushroom Cultivation Medium against Phytopathogenic Fungi Masrukhin Masrukhin; Ade Lia Putri; Tri Ratna Sulistiyani; Muhammad Ilyas; Ismu Purnaningsih; Iwan Saskiawan; Muhammad Yusrun Niam
Journal of Tropical Biodiversity and Biotechnology Vol 6, No 1 (2021): April
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.59235

Abstract

Several bacteria were isolated from straw mushroom (Volvariella volvacea) cultivation medium. There are three potential isolates previously characterized and has growth inhibition effect against V. volvacea. This screening result lead to the further study about the inhibition activity against phytopathogenic fungi. The aim of this research is to investigate the antifungal activity of three bacterial isolates against three phytopathogenic fungi and identification of the bacteria. The method used in this study are antifungal assay using co-culture method and disk difussion assay using the filtrate of each bacteria. The profile of antifungal compound was identified using ethyl acetate extract followed by evaporation and gas chromatography (GC-MS) analysis. Identification of each isolates was performed using 16S rDNA amplification and sequencing. Three phytopathogenic fungi i.e Cercospora lactucae (InaCC F168), Colletotrichum gloeosporides (InaCC F304) and Fusarium oxysporum f.sp. cubense (F817) were co-cultured with bacterial isolates C2.2, C3.8, and D3.3. The C3.8 isolate has highest average inhibition activity either using isolate and filtrate. The result relatively consistent against three phytopathogenic fungi. The metabolite profile of C3.8 isolate showed the Bis(2-ethylhexyl) phthalate as the main compound with 97% similarity. Bis(2-ethylhexyl) phthalate has potential effect as antibacterial and antifungal compound. According to EzBioCloud and GeneBank databases, the C2.2 isolate identified as Bacillus tequilensis, C3.8 as Bacillus siamensis and D3.3 as Bacillus subtilis subsp. Subtilis. This study also shows the potential of Bacillus siamensis C3.8 as biocontrol against phytopathogenic fungi.
OPTIMASI PRODUKSI SERTA ANALISIS AKTIVITAS ANTIOKSIDAN DAN ANTIMIKROBA SENYAWA EKSOPOLISAKARIDA DARI JAMUR TIRAM PUTIH (Pleurotus ostreatus) PADA MEDIA CAIR Iwan Saskiawan; Misbahul Munir; Suminar S Achmadi
BERITA BIOLOGI Vol 15, No 2 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i2.2299

Abstract

Pleurotus ostreatus is well known as an oyster mushroom that is very popular because of its high nutritional value and pharmaceutical component. The aim of this research was to study the antioxidant and antibacterial activity of exopolysaccharides from P. ostreatus grown on liquid medium.The P. ostreatus was grown on Mushroom Complete Medium (MCM) containing various types of Carbon (C) and Nitrogen (N) sources, i.e glucose, lactose, amylose and sucrose as a Carbon sources and yeast extract, polypeptone, NH4Cl, and NH4NO3 as a Nitrogen sources. The results showed that sucrose and yeast extract were the best source of Carbon and Nitrogen that produced 208 and 100 mg/L of exopolysaccharides.  The exopolysaccharide exhibited antimicrobial activity against Bacillus subtilis and Escherichiacoli. However, it did not have antimicrobial activity against Saccharomyces cerevisiae.  In addition, the exopolysaccharide indicated to have an antioxidant activity.
Utilization of coconut water as inducing substance in the cultivation of Pleurotus ostreatus Elfarisna Elfarisna; Iwan Saskiawan
Jurnal AGROSAINS dan TEKNOLOGI Vol 4, No 1 (2019)
Publisher : Fakultas Pertanian - UMJ

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (178.093 KB) | DOI: 10.24853/jat.4.1.25-30

Abstract

Cultivation of edible mushrooms has been evaluated as an effective way for increasing income of people in developing countries. Pleurotus ostreatus, well known as an oyster mushroom is the most popular edible mushroom in Indonesia. They usually is cultivated using a sawdust as a main substrate. The aim of this research is to reveal the addition of coconut water, the byproduct of traditionally coconut process as an inducing substance in P. ostreatus cultivation.  The results shown that the addition 25% (v/v) of coconut water in substrate of P. ostreatus induce the growth of mycelium. On the other hand, the addition of 50% (v/v) of coconut water affect in the day of fruit body formation become faster as well as increase the number of pileus (stalk)  and  the weight of fruit body.
Co-Authors Ade Lia Putri ELFARISNA ELFARISNA Ismu Purnaningsih Masrukhin Masrukhin Misbahul Munir Muhammad Ilyas Muhammad Yusrun Niam Suminar S Achmadi Tri Ratna Sulistiyani