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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 24 Documents
 1   2   3 
Search results for , issue "Vol 16, No 2 (2017)" : 24 Documents clear
PERBANYAKAN VEGETATIF BIDARA UPAS (Merremia mammosa (Lour.) Hallier f) KEBUN RAYA BOGOR [Vegetative Propagation of Bidara Upas ( Merremia mammosa (Lour.) Hallier f)) at Bogor Botanical Garden] Cahyaningsih, Ria; Hidayat, Syamsul; Hidayat, Endang
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3299.561 KB) | DOI: 10.14203/beritabiologi.v16i2.2284

Abstract

Bidara upas (Merremia mammosa (Lour.) Hall.f.) is a medicinal plant, , that traditionaly use by Indonesian and Malaysian people. Regarding Indonesia Biodiversity Strategy and Action Plan released by The National Development Planning Agency, it is included in the extinct medicinal plant list (2003). As the center for plant conservation, Bogor botanical garden conducted vegetative propagation study on this plant by air layering, cutting, and tuber cutting. The aim to this study is to find effective for bidara upas which method is vegetative propagation. Air layering and cutting propagation modified by Rootone-F did not give any result, whereas tuber cutting propagation with GA3 gave positive result. Principally, propagation by tuber cutting modified by 50 ppm of GA3 dose gave the most rapid and highest uniformity on shoot emergence, the best on growing variables, namely average height of shoot growing, the number of secondary roots, the number and length of the primary roots. It cannot be concluded yet that propagation by tuber cutting modified by GA3 was the most effective. Hence, it is necessary to conduct another propagation studies of air layering and cuttings with more variants of treatments, especially Rootone-F dose.
HERITABILITAS DAN PEROLEHAN GENETIK PADA BOBOT IKAN NILA HASIL SELEKSI [Heritability and Genetic gain on Weight of Tilapia Resulted Frown Individual Selection] Nugroho, Estu; Mayadi, Lalu; Budileksono, Sigit
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3300.024 KB) | DOI: 10.14203/beritabiologi.v16i2.2689

Abstract

Directional-individual selection on tilapia growth had been conducted in West Nusa Tenggara Province. The base population had been constructed through di allele crossing between six tilapia strains i.e. NIRWANA, BEST, SULTANA, Citralada, JATIMBULAN and White Sleman. A total of 150 brood stock pairs were used for spawning with the ratio of female and male parent 1: 1 in net with size of 1x1x1 m. Two hundred larvae  produced from each spawn pairs were communally reared in a pond for three months. Directional-individual selection was conducted at the size of > 50 grams. The cut-off for minimum selected fish was based on the lowest individual weight of 10% top population. The average value of heritability on body weight was 0.251 for males and 0.258 for the female population. The total value of genetic gain of body weight for four generations was 51.68% for male population and 56.78% for the female population.
Pellacalyx Symphiodiscus STAFP FROM LONG BAGUN, MAHAKAM HULU: MORPHOLOGICAL CHARACTERIZATION AND ITS DISTRIBUTION Inggit Puji Astuti; Ratna Susandarini; Rismita Sari
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v16i2.2650

Abstract

Pellacalyx is one of the genera in Rhizophoraceae, distributed from Myanmar, South China, Indonesia, Malaysia and the Philippines. In Indonesia, Pellacalyx can be found in the island of Sumatra, Kalimantan and Sulawesi. Eight species of Pellacalyx have been found  in Indonesia, four of which them were from Kalimantan, i.e. P. axillaris, P. cristatus, P. lobbii and P. symphiodiscus. These four species generally grow in the primary and secondary forest, from lowland to an altitude of 1300 m above sea level. Information of Pellacalyx in Indonesia is still very limited , and rarely found in the wild. The last information of Pellacalyx found in West Sumatra was reported by an expedition team of the Center for Plant Conservation Bogor Botanical Garden in 2006.  The Expert Team of the Khatulistiwa Expedition in Long Bagun, West Kutai, East Kalimantan on May 26–June 2, 2012 found a species of Pellacalyx. Morphological characterization was then conducted based on the herbarium specimen for identification. The identification was carried out in Bogor Botanic Gardens, Center for Biological Research, Indonesian Institute of Sciences and The National Herbarium Leiden, Netherland. The results show that Pellacalyx specimen from Long Bagun is Pellacalyx symphiodiscus. 
VARIASI GENETIK Lactobacillus fermentum Beijerink ASAL SAYUR ASIN BERDASARKAN ANALISIS RFLP 16S-23S rDNA ISR, RAPD-PCR DAN ERIC-PCR Sulistiani Sulistiani; Wibowo Mangunwardoyo; Abinawanto Abinawanto; Endang Sukara; Achmad Dinoto; Andi Salamah
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v16i2.2772

Abstract

Molecular analysis of Lactobacillus fermentum isolates is essential to understand their genetic variation in relations to their roles in sayur asin fermentation process. Combination of three molecular techniques which is restriction fragment length polymorphism (RFLP) of 16S23S rDNA intergenic spacer region (ISR), random amplified polymorphic DNA (RAPD-PCR) and an enterobacterial repetitive intergenic consensus (ERIC-PCR) analysis were performed to discriminate 19 representative isolates of L. fermentum isolated from sayur asin. The result showed that L. fermentum strain D11 is distantly related to other isolates based on RFLP using HhaI restriction enzyme and RAPDPCR analyses. In addition, both of RAPD-PCR and ERIC-PCR successfully determined the genetic variation among L. fermentum strains by exhibiting distinct 4-8 bands (800-2080 bp) and 4-10 bands (280-3050 bp), respectively. A dendogram generated from UPGMA cluster analysis of both RAPD-PCR and ERIC-PCR data showed two distinct genotypic groups exist among L. fermentum isolated from sayur asin in Indonesia.
PERBANYAKAN VEGETATIF BIDARA UPAS (Merremia mammosa (Lour.) Hallier f) KEBUN RAYA BOGOR Ria Cahyaningsih; Syamsul Hidayat; Endang Hidayat
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v16i2.2284

Abstract

Bidara upas (Merremia mammosa (Lour.) Hall.f.) is a medicinal plant, , that traditionaly use by Indonesian and Malaysian people. Regarding Indonesia Biodiversity Strategy and Action Plan released by The National Development Planning Agency, it is included in the extinct medicinal plant list (2003). As the center for plant conservation, Bogor botanical garden conducted vegetative propagation study on this plant by air layering, cutting, and tuber cutting. The aim to this study is to find effective for bidara upas which method is vegetative propagation. Air layering and cutting propagation modified by Rootone-F did not give any result, whereas tuber cutting propagation with GA3 gave positive result. Principally, propagation by tuber cutting modified by 50 ppm of GA3 dose gave the most rapid and highest uniformity on shoot emergence, the best on growing variables, namely average height of shoot growing, the number of secondary roots, the number and length of the primary roots. It cannot be concluded yet that propagation by tuber cutting modified by GA3 was the most effective. Hence, it is necessary to conduct another propagation studies of air layering and cuttings with more variants of treatments, especially Rootone-F dose.
HERITABILITAS DAN PEROLEHAN GENETIK PADA BOBOT IKAN NILA HASIL SELEKSI Estu Nugroho; Lalu Mayadi; Sigit Budileksono
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v16i2.2689

Abstract

Directional-individual selection on tilapia growth had been conducted in West Nusa Tenggara Province. The base population had been constructed through di allele crossing between six tilapia strains i.e. NIRWANA, BEST, SULTANA, Citralada, JATIMBULAN and White Sleman. A total of 150 brood stock pairs were used for spawning with the ratio of female and male parent 1: 1 in net with size of 1x1x1 m. Two hundred larvae  produced from each spawn pairs were communally reared in a pond for three months. Directional-individual selection was conducted at the size of > 50 grams. The cut-off for minimum selected fish was based on the lowest individual weight of 10% top population. The average value of heritability on body weight was 0.251 for males and 0.258 for the female population. The total value of genetic gain of body weight for four generations was 51.68% for male population and 56.78% for the female population.
LUMUT SEJATI DI HUTAN ALAM PAMEUNGPEUK, TAMAN NASIONAL GUNUNG HALIMUN SALAK, JAWA BARAT Florentina Indah Windadri
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v16i2.2302

Abstract

Halimun Salak National Park is one of the remaining conservation areas left in West Java. Research on diversity of Bryophytes had previously been conducted at  Halimun Mount, Kendeng Mount and  the lowland forest surrounding Cikaniki-Citalahab, while this present  study was aimed to  record the diversity of mosses at Pameungpeuk primary forest. There were 78 species  of mosses including in 47 genera and 20 families. Syrrhopodon spiculosus Hook. & Grev. was a dominant species in the research area. Twenty seven species were new record for Halimun Salak National Park, one of which (Rhizogonium lamii Reimers) was a new record for Java and seven species were new record for Indonesia.
STUDI BIOMETRI BERDASARKAN MERISTIK DAN MORFOMETRIK IKAN GURAMI GALUR BASTAR DAN BLUESAFIR Deni Radona; Nunak Nafiqoh; Otong Zenal Arifin
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v16i2.2758

Abstract

Identification of Gouramy is required to obtain phenotype characteristics information of the population for sustainable aquaculture  practices. In this study phenotype diversity of giant gouramy strain bastar and bluesafir was analyzed based on  meristic and morphometric measurement. Sample, are taken from 30 individuals of each population. The result showed that there were differences in colour patterns between the two populations, bastar population had yellowish brown body and red fins, whilst bluesafir population had brown blackish brown body and fins. The canonical function of truss morphometric analysis on 21 characteristics showed the measurement distribution of that both populations were in different quadrants. The intrapopulation similarity index showed that population of strain bluesafir had higher genetic similarity (80%) with interpopulation similarity index of 23.3%, while the intrapopulation similarity index of strain bastar was 76.7%.
PATOGENISITAS ISOLAT BAKTERI Xanthomonas oryzae pv. oryzae DAN PEMANTAUAN PENYAKIT HAWAR DAUN BAKTERI PADA PADI GALUR ISOGENIK Yadi Suryadi; Triny Suryani Kadir
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v16i2.2393

Abstract

Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is an important rice disease due to its high intensity that and  the mereased durability of its resistant variety. This study was aimed  to determine the pathogenicity of Xoo isolates, which obtained from four regions/districts in West Java on three rice near isogenic lines (NILs) containing a single resistance (R) gene; and  to study reaction of rice NILs/ differential genotypes containing mixture of resistant genes to Xoo population under endemic areas. Out of 22 Xoo isolates have been collected and further determined by ELISA assay. Ten Xoo isolates were selected and inoculated to identify their pathogenicity on three NILs i.e., IRBB5 (xa5), IRBB7 (Xa7) and IRBB21 (Xa21). Pathogenicity test showed that most of isolates produced large lesion, and four virulence groups were identified. Phylogenetic analysis suggested that the dominant virulent isolates were widely distributed at several district in West Java. It was shown that 11 NILs exhibited high levels of resistant reaction to the predominant Xoo pathotypes in Cianjur, while 10 lines were susceptible. The single R gene (IRBB7, IRBB21 and Java14) exhibited lower BLB severity. The IRBB50, IRBB51, IRBB52, IRBB53 lines (carrying two R genes), IRBB56, IRBB57 (carrying three R genes); IRBB64 (carrying four R genes), and IRBB66 (carrying five R genes) showed lower severity and thus produced higher resistance to Xoo. This study may implied further work to deploy effective R genes against certain Xoo pathotypes in differ  region.   
KARAKTERISASI ENZIM PROTEASE DARI BAKTERI Stenotrophomonas sp. ASAL GUNUNG BROMO, JAWA TIMUR Yati Sodaryati Soeka; Sulistiani Sulistiani
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v16i2.2940

Abstract

Protease is an enzyme that can hydrolyze protein into simpler compounds, i.e peptides and amino acids. Microbial Proteases have the  potency to be applied in industries such as detergents, skins, silver recovery, dairy, baking, beverages and pharmaceutical industries. These hydrolytic enzyme are efficiently involved in the food industry to increase the nutritional value, digestibility, palatability, flavour and reducing allergenic compounds as well as in the management of domestic and industrial wastes. The purpose of this study was to investigate the ability of Stenotrophomonas sp. isolated from Mount Bromo, East Java in producing protease. Protease activity of the bacterial isolate was qualitatively determined by formation of a clear zone surrounded their colonies on media containing skim milk (1%). We analyzed its  proteolic activity against some effects of the incubation period, pH, temperatures and addition of monovalent and divalent metal ionsquantitatively using a spectrophotometer at ? 280 nm.The results showed that the optimum activity after incubation for two days was 315.88 U/ mL. The enzyme has continued to its activity at pH 8 (419.68 U/mL) and maintained its stability at 398.22 U/mL with activities decreased to 94.87%, while its activity at 60°C was 519.86 U/mL and could maintain its stability at 419.58 U/ mL, the activity decreased to 74.75%. The addition of Ca2+ could activated its enzyme activity at the amount of 424.33U/mL, while without addition of the ion its activity was 400.29 U/mL. The addition with ion Mn²+, K+, Na+ and Cu 2+ could act as inhibitors that might reduced the activity of the enzyme.  

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