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All Journal Agriwar Journal Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Novemia Fatmarischa
Department of Animal Science, Faculty of Agriculture, Mulawarman University, Samarinda, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions

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Physiological Responses of Bali Cattle Associated with Temperature-Humidity Index under Tropical Environmental Conditions Kirana Dara Dinanti Adiputra; Sukandi; Cori Qamara; Novemia Fatmarischa; I Putu Gede Didik Widiarta
Agriwar Journal Vol. 5 No. 2 (2025): Agriwar Journal
Publisher : Program Studi Magister Sains Pertanian Program Pascasarjana Universitas Warmadewa

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The temperature-humidity index (THI) is an indicator that combines air temperature and humidity to assess the level of comfort or heat stress in livestock. This study aims to evaluate the relationship between the physiological response of Bali cattle to THI in tropical areas. A total of 20 Bali cattle (10 males and 10 females) were observed in the morning and afternoon. Microclimatic data (temperature, humidity, THI) and physiological responses, including rectal temperature (RT), skin surface temperature (ST), respiratory rate (RR), and heart rate (HR), were analysed using the General Linear Model (GLM) with repeated measures and linear regression. The results showed significant differences (P<0.01) between morning and afternoon. THI increased from 77.34 ± 0.82 (mild heat stress) in the morning to 86.04 ± 1.20  (severe heat stress) in the afternoon. Physiological responses increased significantly in the afternoon, with males showing higher RT and RR than females. Regression analysis indicated that THI was closely related to RT (R² = 0.8822), RR (R² = 0.8065), and HR (R² = 0.9454), while ST showed a moderate relationship (R² = 0.5503). It can be concluded that Bali cattle are sensitive to tropical climate fluctuations, and THI can be used as an indicator to assess heat stress levels in Bali cattle.
ANALYSIS OF Deoxyribonucleic Acid (DNA) FRAGMENTATION AND ACROSOME INTEGRITY AS BIOMARKERS OF FROZEN SEMEN QUALITY OF BULLS AT THE SPERM BANK OF SAMARINDA CITY Kirana Dara Dinanti Adiputra; Novemia Fatmarischa; Dani Nur Arifin; Fikri Ardhani; Kumbawan Wibisono; Novi Ramadhani; Erni Damayanti
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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This study aims to evaluate the quality of frozen bull semen at the Sperm Bank of Samarinda City based on parameters of motility, viability, morphological normality, plasma membrane integrity (PMI), acrosome integrity, and DNA fragmentation, as well as their effects on cattle breeds and storage duration. The samples consisted of frozen semen from five breeds (Limousin, Madura, Angus, Simmental, and Peranakan Ongole) with different storage periods (3, 4, and 5 years). Motility, viability, and morphological normality were tested according to the Indonesian National Standards (SNI). The PMI was assessed using the hypo-osmotic swelling test (HOST), acrosome integrity using peanut agglutinin (PNA) staining, and DNA fragmentation using acridine orange (AO) staining. Data were analyzed using two-way ANOVA, and if there was an effect, it was followed by a Tukey post hoc test. The findings indicated that motility and PMI remained relatively stable; however, the duration of storage significantly influenced PMI (P<0.05). The breed of bull and storage duration had a significant effect on morphological normality, viability, acrosome integrity, and DNA fragmentation (P<0.05). However, the two factors did not interact with each other. It can be concluded that the storage duration resulted in a decline in semen quality, specifically in terms of membrane and DNA integrity. Therefore, it is recommended to use semen straws ≤ 4 years old and conduct periodic quality re-qualification to maintain frozen semen fertility.
ANALYSIS OF Deoxyribonucleic Acid (DNA) FRAGMENTATION AND ACROSOME INTEGRITY AS BIOMARKERS OF FROZEN SEMEN QUALITY OF BULLS AT THE SPERM BANK OF SAMARINDA CITY Kirana Dara Dinanti Adiputra; Novemia Fatmarischa; Dani Nur Arifin; Fikri Ardhani; Kumbawan Wibisono; Novi Ramadhani; Erni Damayanti
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

This study aims to evaluate the quality of frozen bull semen at the Sperm Bank of Samarinda City based on parameters of motility, viability, morphological normality, plasma membrane integrity (PMI), acrosome integrity, and DNA fragmentation, as well as their effects on cattle breeds and storage duration. The samples consisted of frozen semen from five breeds (Limousin, Madura, Angus, Simmental, and Peranakan Ongole) with different storage periods (3, 4, and 5 years). Motility, viability, and morphological normality were tested according to the Indonesian National Standards (SNI). The PMI was assessed using the hypo-osmotic swelling test (HOST), acrosome integrity using peanut agglutinin (PNA) staining, and DNA fragmentation using acridine orange (AO) staining. Data were analyzed using two-way ANOVA, and if there was an effect, it was followed by a Tukey post hoc test. The findings indicated that motility and PMI remained relatively stable; however, the duration of storage significantly influenced PMI (P<0.05). The breed of bull and storage duration had a significant effect on morphological normality, viability, acrosome integrity, and DNA fragmentation (P<0.05). However, the two factors did not interact with each other. It can be concluded that the storage duration resulted in a decline in semen quality, specifically in terms of membrane and DNA integrity. Therefore, it is recommended to use semen straws ≤ 4 years old and conduct periodic quality re-qualification to maintain frozen semen fertility.
Co-Authors Cori Qamara Dani Nur Arifin Erni Damayanti Fikri Ardhani I Putu Gede Didik Widiarta Kirana Dara Dinanti Adiputra Kirana Dara Dinanti Adiputra Kumbawan Wibisono Novi Ramadhani Sukandi