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All Journal BIOEDUSCIENCE Biocaster : Jurnal Kajian Biologi
Afiya, Dina
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology

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Validitas E-Modul Biologi Berbasis PBL Isu Lingkungan Lokal Tuban untuk Meningkatkan Kemampuan Berpikir Kritis Siswa Afiya, Dina; Indrati, Dika Agustia
Biocaster : Jurnal Kajian Biologi Vol. 6 No. 2 (2026): April
Publisher : Lembaga Pendidikan, Penelitian, dan Pengabdian Kamandanu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/biocaster.v6i2.1299

Abstract

This study aims to develop a Problem-Based Learning (PBL)-based biology e-module with a local theme from Tuban Regency, focusing on environmental issues. The study used a Research and Development (R&D) model with ADDIE stages limited to analysis, design, and development. The data collection instrument was a validation sheet that assessed the feasibility of the material, systematics, language, and media design and presentation. The results showed that the PBL-based biology e-module with a local theme from Tuban met the criteria of highly valid, with an average score of 81.75% from biology learning media expert validators and 97.5% from science learning material expert validators (biology). Based on these results, the developed e-module is suitable as a supporting teaching material in biology lessons for seventh-grade junior high school students on environmental issues with a local theme.
Identification and Characterization of Pathogenic Fungi Causing Necrosis in Vigna unguiculata Afiya, Dina; Mustofa, Ali; Nurtjahyani, Supiana Dian
BIOEDUSCIENCE Vol 10 No 1 (2026): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/18508

Abstract

Background: Vigna unguiculata (cowpea) is an important legume crop whose productivity can be affected by foliar diseases, including leaf necrosis caused by fungal pathogens. Identification of fungi associated with necrotic symptoms is essential as baseline information for plant disease management. Methods: This study aimed to identify fungi associated with necrotic lesions on cowpea leaves. Leaf samples showing necrotic symptoms were collected from agricultural fields in Tengger Wetan Village, Kerek Subdistrict, Tuban Regency, East Java. Fungal isolation was performed from infected leaf tissues to obtain pure cultures. Identification was conducted based on macroscopic colony characteristics and microscopic features using slide culture techniques and standard morphological identification keys. Results: Two dominant fungal isolates were consistently associated with necrotic symptoms on cowpea leaves. These fungi were identified morphologically as Alternaria alternata (isolate D1) and Nigrospora sp. (isolate D3). A. alternata was characterized by yellowish-white colonies, hyaline septate hyphae, and dark oval conidia, whereas Nigrospora sp. exhibited cottony white colonies with brown, globose conidia. Both fungi were associated with brown to black necrotic spots on the leaf surface. Conclusions: The findings provide initial information on fungal species associated with leaf necrosis in cowpea based on morphological identification. This information is useful as a foundation for further pathological and molecular studies to confirm species identity and support the development of appropriate disease management strategies.
Co-Authors Ali Mustofa Indrati, Dika Agustia Supiana Dian Nurtjahyani