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All Journal Microbiology Indonesia Biomedical Engineering Jurnal Florea Al Ishlah Jurnal Pendidikan JPM (Jurnal Pemberdayaan Masyarakat) BIOEDUSCIENCE Jurnal SOLMA Jurnal Penelitian dan Pengkajian Ilmu Pendidikan: e-Saintika Bubungan Tinggi: Jurnal Pengabdian Masyarakat Indian Journal of Forensic Medicine & Toxicology Berkala Penelitian Hayati Media Didaktika Journal of Biodiversity and Biotechnology Jurnal Penelitian Pendidikan Indonesia (JPPI) Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Edumaspul: Jurnal Pendidikan

Supiana Dian Nurtjahyani

Universitas PGRI Ronggolawe

Author-ID : 335767

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

Published : 23 Documents Claim Missing Document

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Phylogenetic Analysis Related to Hepatitis B Virus based on Region Surfaces in Tuban, East Java Province, Indonesia Supiana Dian Nurtjahyani; Retno Handajani
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i4.17103

Hepatitis B virus (HBV) is a virus cause liver disease. This virus could be transmitted through blood product,and it becomes the major problems in developing countries. Genotype distribution of HBV depends ongeographical characteristics. Here, we reported HBV genotyping analysis of blood donors with positiveHepatitis Surface Antigen (HbsAg) at Indonesian Red Cross Foundation in Tuban, East Java. Total 150sera were collected from blood donors at Indonesian Red Cross Foundation in Tuban, East Java. Enzyme-Linked Immunosorbent Assay (ELISA) was conducted by PRISM®HBsAg kit (ABBOTT) according tothe manufacture’s instruction, to determine sample that has positive HbsAg. The genotyping analysis wasdone using polymerase chain reaction and sequencing method from 12 samples. Genetyx ver.10 softwarewas used for identification of HBV type. Analysis of HBV genotype revealed three different types of HBV.HBV type B was the major HBV (50%). Interestingly, there was one unidentified HBV type suggested as anovel subtype of HBV. Further genotyping analysis is needed to confirm novelty of the unidentified HBVtype. Analysis of HBV genotype revealed three different types of HBV. HBV type B was the major HBV(50%). Interestingly, there was one unidentified HBV type suggested as a novel subtype of HBV. Furthergenotyping analysis is needed to confirm novelty of the unidentified HBV type. This study was the firstevidence of blood donor’s genotyping analysis in Tuban.

Detection of Hepatitis C Virus RNA in Blood Donors with Nested PCR Technique Supiana Dian Nurtjahyani; Mochammad Amin Amin; Retno Handajani Handajani
Biomedical Engineering Vol 2, No 2 (2016): Biomedical Engineering
Publisher : Biomedical Engineering

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (448.443 KB)

Objective: This study was conducted to detect hepatitis C virus (HCV) RNA among blood donors in Tuban, East Java, Indonesia, using nested Polymerase Chain Reaction (PCR) technique.Material and methods: Seven blood donors with positives anti-HCV were obtained from 500 blood donors from Red Cross Blood Donor Center (Palang Merah Indonesia = PMI) Tuban, East Java, Indonesia, consisting of 375 men (75%) and 125 women (25%). Detection of anti-HCV antibody prevalence in the blood donors was done by using Enzyme-Linked Immunosorbent Assay (ELISA). The detection of HCV RNA in blood donors sera with positives anti-HCV were performed using nested-PCR technique with three set of primer pairs targeting the NS5B and 5’UTR regions of HCV. Further amplification products were visualized on a 2% agarose gel containing ethidium bromide under UV illumination.Result: Seven positives anti-HCV blood donors sera (1.4%) were obtained from 500 blood donors sera, in PMI Tuban, East Java, Indonesia. Out of seven blood donors sera with positives anti-HCV, 6 sera (85,7%) were positives HCV RNA by using nested PCR technique. Five HCV RNAs were detected with two set of primers based on NS5B HCV region and 1 positive HCV RNA was detected with another set of primer based on 5’UTR HCV region.Conclusion: HCV RNAs were detected in 85,7% positives anti-HCV blood donors using nested PCR technique, in PMI Tuban, East Java. Further research is needed to determine the genotype and subtype of HCV.

Detection of Gen Chloramphenicol Acetyl Transferase (CAT ) Salmonella typhi Resistant Chloramphenicol by Polymerase Chain Reaction (PCR) Technique Supiana Dian Nurtjahyani; Retno Handajani
Biomedical Engineering Vol 3, No 1 (2017): Vol. 3 No. 1 Biomedical Engineering
Publisher : Biomedical Engineering

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (334.416 KB)

Objective: Salmonella typhi as a causative agent of typhoid abdominalis many militias, which was resistant to the drug, one of which is chloramphenicol. Detection of Salmonella typhi serologically generally done so if there is a problem in the diagnosis difficult to overcome so it needs a faster diagnosis techniques with accurate results. PCR can be used as an alternative solution to these problems. The purpose of this study was to detect the DNA of Salmonella typhi resistant to chloramphenicol by PCR.Material and methods: The method in this population experimental laboratory conducted in the laboratory biology and laboratory Unirow Tuban ITD Airlangga University Surabaya. Experiment done using PCR from blood samples. Using primer from CAT gene to detect this gene.Result: The results of this study with tank PCR can detect DNA of chloramphenicol resistant Salmonella typhi with a length of 293 bp.Conclusion: The conclusion PCR technique can detect DNA of Salmonella typhi resistant to chloramphenicol.

A STUDY OF TYPE AND INTENSITY OF DISEASE INFECTING BANANA PLANTS Musa sp AT TEGALAGUNG VILLAGE SEMANDING SUBDISTRICT Supiana Dian Nurtjahyani; Devi Shyntya Agustin
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 2 (2014): June 2014
Publisher : The East Java Biological Society

Diseases affecting banana plants are very detrimental to farmers as these can lower production and economic income. The purpose of this study was to determine the type and intensity of the disease affecting banana plants. This research was an observational analytic study that observe and analyze condition or symptoms of diseases affecting banana plants in Tegalagung village, Semanding subdistrict, Tuban as many as 38 samples. Parameters observed were type of disease and measure intensity of the disease, data obtained were analyzed descriptively. Based on the symptoms that occurred on the leaves, the study found four disease types affecting banana plant that were fusarium wilt, bacterial wilt (Blood), Sigatoka leaf spot and stunting disease. The diseases intensity were 50% of Fusarium wilt; 26,66% of bacterial wilt (Blood); 26.32% of Sigatoka leaf spot and 15.38% of stunting disease. Conclusion of the study, the highest intensity of the disease that attacks banana plants is Fusarium wilt as high as 50%.

The Implementation of TPACK Learning in Human Movement Systems Materials to Improve Students’ Critical Thinking Supiana Dian Nurtjahyani; Anik Winarni; Indra Sugiarsi; Restiani Agusvita; Ali Mustafa; Sukisno Sukisno
AL-ISHLAH: Jurnal Pendidikan Vol 14, No 4 (2022): AL-ISHLAH: Jurnal Pendidikan
Publisher : STAI Hubbulwathan Duri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35445/alishlah.v14i4.2287

Natural Sciences is the study of natural phenomena. In science learning, students are expected to have scientific knowledge, high-level thinking, critical and creative thinking, so that they become independent learners. The purpose of this study was to describe students' critical thinking skills through environmental-based TPACK learning on the human motion system material. This research method is done by lesson study with 3 learning cycles. The results showed that TPACK learning can improve students' critical thinking, especially in identifying, linking, analyzing and concluding in this study the application of TPACK learning to improve thinking skills in categories can be very good.

Genotype of Hepatitis B Virus Coinfection in Typhoid Patients SUPIANA DIAN NURTJAHYANI; RETNO HANDAJANI
Microbiology Indonesia Vol. 9 No. 3 (2015): September 2015
Publisher : Indonesian Society for microbiology

Typhoid fever can cause liver disorder and may result in complication. Studies revealed hepatic involvement in thypoid known as typhoid hepatitis. Our previous paper reported the existance of hepatitis B virus (HBV) coinfection in the serum of patients with typhoid using nested-polymerase chain reaction (PCR). Based on sequence divergence, HBV has been classified into 10 genotypes (A-J), which in prediction response and correlates with clinical outcome of chronic HBV infection. This study was conducted to determine the genotypes of HBV in typhoid patients coinfected with HBV in Tuban. Sera obtained from 5 typhoid patients positive HBV by nested PCR). Study was performed by direct sequencing BigDye V1.1 Terminator Cycle Sequencing kit and ABI Prism 310 Genetic Analyzer. Analys had been using the Genetix version 10 software to create the phylogenetic tree. Phylogenetic analysis showed 3 samples as genotype B and 2 two samples as genotype C.

Prevalence of Hepatitis B Virus Infection in Blood Donors Based on Titer Hepatitis B Surface Antigen Examination (HBsAg) SUPIANA DIAN NURTJAHYANI; RETNO HANDAJANI
Microbiology Indonesia Vol. 13 No. 4 (2019): December 2019
Publisher : Indonesian Society for microbiology

Hepatitis B remains a global public health problem. Infection from hepatitis B virus (HBV) can be transmitted through a blood test or a blood transfusion. This study was conducted to identify the prevalence of HBV infection in blood donors based on examination of HBsAg titers . Blood donors from Tuban Red Cross used as sample. The method used in this research is HBsAg titers examination performed by ELISA according to the procedureoutlined in the Kit. HBsAg titers positive mostly found in men. In men from 13 samples (8.67%) are HBsAg titers positive of 150 samples while in woman all negative for HBsAg titers from 137 samples. The average titer positive was 3.095 with a standard deviation of 0.187. While HBsAg titers negative have average of 0.03 with a standard deviation of 0.14. This study showed that the prevalence of HBV infection in blood donors is most numerous in men with HBsAg titers positive number of 8.67%.

Utilization of Leaves in Mine Reclamation Land as Organic Fertilizer with Effective Bioactivatory of Microorganism 4 (em4) and Molasses Supiana Dian Nurtjahyani; Dwi Oktafitria; Sriwulan Sriwulan; Nova Maulidina; Imas Cintamulya; Eko Purnomo
Microbiology Indonesia Vol. 14 No. 2 (2020): June 2020
Publisher : Indonesian Society for microbiology

Organic fertilizer as an alternative to reduce the scarcity of chemical fertilizers, otherwise it can improve soil fertility. PT. Semen Gresik - Semen Indonesia Persero (Tbk.) Has a lot of plants in the treatment period in ex-mining land that needs fertilizer to help plant growth. In addition, in post-mining land, there is a lot of organic waste in the form of dry leaves under the stands of reclamation trees. The organic waste has the potential to be processed and used as organic fertilizer. This study aims to determine the utilize leaf litter in the reclaimed mine land as organic fertilizer with EM 4 and Molasses bioactivators. This study used is a laboratory experimental method. The results showed the composition of solid material in the form of leaf litter, bran, cow dung and husk charcoal in a ratio of 2: 1: 1: 1 as well as looking material in the form of molasses and EM4 with a dose of 100ml per 100kg of solid material dissolved in enough water (fertilizer can be clenched and expanded slowly and not broken). Conclusion leaf litter in the former reclaimed land can be processed and used as organic fertilizer.

Effective Microorganisms 4 (EM-4) Application in The Process of Making Compost from Household Organic Waste to Increase Skills of PKK Members Tabitha Sri Hartati Wulandari; Supiana Dian Nurtjahyani; Dede Nuraida; Christina Innocenti Tumiar Panggabean; Sukisno Sukisno
Bubungan Tinggi: Jurnal Pengabdian Masyarakat Vol 6, No 2 (2024)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/btjpm.v6i2.10251

Handling the problem of household waste is getting more complicated by the day. Some people do not have a new perspective on dealing with waste. For this reason, serious handling is needed when utilizing household waste as a resource. Household waste is the largest contributor to creating organic waste, so to turn organic waste into resources, it is necessary to work on piles of organic waste from household waste by utilizing waste in compost. The benefits of compost are many in terms of fertilizing plants, so through community service, training activities for making compost with EM 4 were carried out for Baturetno PKK members, where 35 participants attended the training. The aim is to train PKK members skills in handling household organic waste by giving them EM4. In this activity, the methods used were socialization, training, and monitoring. In the training, a questionnaire was distributed to determine the participants' insight and response to the activity. The results of the questionnaire show that there is an increase in understanding and skills as seen from the average answer "YES" before the training of 58.53% with a sufficient category, and after the training to 93% with a very good category, thus indicating that the knowledge and skills of PKK members in Baturetno, Tuban district after training in processing waste from household organic waste into compost with EM 4, has increased.

STUDI PERBANDINGAN PEMAKAIAN PRIMER HBS1 DAN HBS2 DENGAN PRIMER P7 DAN P8 DALAM DETEKSI DNA VIRUS HEPATITIS B PADA PENDONOR DARAH Nurtjahyani, Supiana Dian; Amin, Mohammad; Handajan, Retno
Florea : Jurnal Biologi dan Pembelajarannya Vol 7, No 2 (2020)
Publisher : UNIVERSITAS PGRI MADIUN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25273/florea.v7i2.7887

Examination of the hepatitis B virus as a disease of hepatitis serologically has been carried out a lot, but this serological examination can experience problems if there is a low window period, so even though a negative test result is still possible in a patient's body infected with the hepatitis B virus, a fast and accurate examination is needed. PCR examination is an alternative solution to overcome this problem because the results are more accurate and valid because it can directly detect hepatitis B virus DNA as the cause of hepatitis B infection. This examination requires selecting the right primer so that accurate results are obtained. The aim of this study was to compare the use of HBS1 and HBS2 primers with P7 and P8 primers in the detection of Hepatitis B virus DNA in blood donors. The method used in this study is a laboratory experiment with Polymeration Chain Reaction (PCR) examination. The results showed that the HBS1 and HBS2 primers produced more positive HBV DNA by 76.92% compared to P7 and P8 primers, only 23.08%. Conclusion HBS1 and HBS2 primers can detect positive HBV DNA with more results.

Co-Authors *Ali Mustofa Afiya, Dina Agusti Agusti Ahmad Yogi Pambudi Ahmad Zainal Arifin Ainur Rofieq Ali Mustafa Ali Mustofa Anik Winarni Arga Dias Pratama Dias Pratama Arifin, Ahmad Zaenal Avivi Nur Aina Christina Innocenti Tumiar Pangabean Christina Innocenti Tumiar Panggabean Dede Nuraida Devi Shintya Agustin Devi Shyntya Agustin Dhiaulhaq, M. Rafi Dwi Oktafitria Dwi Oktafitria Dwi Oktafitria Dwi Oktavitria Eko Purnomo Eko Purnomo Eko Purnomo eko purnomo Eko Purnomo Eko Purnomo Endah Mutiara Marhaeni Putri Endri Ulfa Kusmawati IS Fajrina Zulfa Darumiarsi Gea Elina Handajan, Retno Hanifa Aasaa Mufida Heny Sulistyaningrum Imas Cintamulya Indah Yana Indra Sugiarsi Kholisotul Hidayah Miftahus Surur MOCHAMAD AMIN Mochammad Amin Mohammad Amin Muhammad Nadjib Mujahid Nia Nurfitria Nurfitria Nova Maulidina Nova Maulidina Nugroho Budi Utomo Nugroho Budi Utomo Nurrohman Nurrohman Nurrohman, Endrik Nurul Hidayah Rafwan Year Perry Burhan Restiani Agusvita Retno Handajani Riris Suharti Lestari Roimil Latifa Rr. Eko Susetyarini Ruril Rudianto Spanton, Perdana Ixbal Sri Wulan Sri Wulan Sriwulan Sriwulan Sriwulan, Sriwulan Sukisno Sulistiono Sulistiono Tabitha Sri Hartati Wulandari Tegar Kurnia, Dwi Wahyu Nur Susanto Zuli Nofiyanti

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