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Journal : Pro Food

PENGARUH SIRUP GULA CAIR HASIL HIDROLISIS ENZIMATIS DARI SAGU (Metroxylon sp.) SEBAGAI MEDIA FERMENTASI TERHADAP KADAR SEFALOSPORIN C: Effect of Glucose Syrup Results Enzymatic Hydrolysis of Sago (Metroxylon sp.) as Media Fermentation Against Cephalosporins C. Soraya Soraya; Sahri Yanti; Mikhratunnisa Mikhratunnisa
Pro Food Vol. 5 No. 1 (2019): Pro Food (Jurnal Ilmu dan Teknologi Pangan)
Publisher : Fakultas Teknologi Pangan dan Agroindustri, Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (675.095 KB) | DOI: 10.29303/profood.v5i1.90

Abstract

ABSTRACT Liquid sugar syrup which made by sago's starch material can be use as fermentation media for sefalosporin. The hydrolisis is a one way to obtain a liquid sugar syrup itself. The enzymatically hydrolisis divided into two process, they are Liquification and sacharification. The research purpose to understanding the way of hydrolisis sago's starch by enzymatically. Next, glucose quality result. Then the influence of the syrup by enzymatically hydrolisis as fermentation media toward quality of sefalosporin. The sample of this research was taken from PT. Selat Panjang, Riau. In Liquification process, the result showed that the maximum glucose quality was obtained about 3874 ppm from concentration of starch and enzym (60g/L: 300µL) meanwhile, the minimum glucose quality was obtained about 3501ppm from concentration of starch and enzym (40g/L: 200µL). Next, sacharification process (lasting 24-48 hours) maximum glucose quality was obtained about 12070 ppm with duration 48hours of hydrolisa. The addition liquid syrup of hydrolisis with five levels concentration, there are 1.5; 2.0; 2.5; 3.0, and 3.5% affected sefalosporin quality. The maximum quality of sefalosporin about 3709 ppm by concentration lyquid syrup GS 3.0% and minimum quality about 2044 ppm by concentration lyquid syrup Gs 1.5%. Meanwhile, by the positive control (glucose monohidrat) with similar treatment, the sefalosporin's quality was about 2170 ppm. Key words: Sago, Sago Starch, Enzymatic Hydrolysis, Fermentation, Cephalosporins C. ABSTRAK Sirup gula cair dari pati sagu dapat digunakan sebagai media fermentasi sefalosporin. Sirup gula cair dapat dengan cara dihidrolisis. Hidrolisis pati sagu secara enzimatis meliputi proses likuifikasi dan sakarifikasi. Penelitian bertujuan mengetahui cara hidrolisis pati sagu secara enzimatis dan kadar glukosa yang dihasilkan, serta pengaruh sirup gula cair hasil hidrolisis enzimatis sebagai media fermentasi terhadap kadar Sefalosporin C. Sampel pati sagu diperoleh dari PT Selat Panjang, Riau. Hasil analisis menunjukkan bahwa pada tahap likuifikasi (2 jam) diperoleh kadar glukosa maksimum sebesar 3874 ppm dari konsentrasi pati sagu dan enzim (60g/L:300µL). Sedangkan kadar glukosa minimum diperoleh sebesar 3501 ppm dari konsentrasi pati sagu dan enzim (40g/L:200µL). Pada tahap sakarifikasi (24-48 jam) kadar glukosa maksimum diperoleh sebesar 12070 ppm dengan waktu hidrolisa 48 jam. Dalam penambahan sirup gula cair hasil hidrolisis dengan lima level konsentrasi sirupgula cair 1,5; 2,0; 2,5; 3,0 dan 3,5% mempengaruhi kadar Sefalosporin C. Kadar maksimum Sefalosporin C dihasilkan sebesar 3709 ppmdari konsentrasi sirup gula cair GS 3,0% dan kadar minimum diperoleh sebesar 2044 ppm dari konsentrasi sirup gula cair GS 1,5%. Sedangkan pada kontrol positif (glukosa monohidrat) dengan perlakuan yang sama diperoleh kadar Sefalosporin C sebesar 2170 ppm. Kata kunci: Sagu, Pati Sagu, Hidrolisis enzimatis, Fermentasi,Sefalosporin C.
PENGARUH MODIFIKASI CROSSLINK TERHADAP KARAKTERISTIK TEPUNG UBI JALAR SAAT DIPANASKAN: Crosslink Modification Effect on the Characteristics of Sweet Potato Flour When Heated Yustiawan Yustiawan; Heru Pitria Hastuti; Sahri Yanti
Pro Food Vol. 5 No. 1 (2019): Pro Food (Jurnal Ilmu dan Teknologi Pangan)
Publisher : Fakultas Teknologi Pangan dan Agroindustri, Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (444.839 KB) | DOI: 10.29303/profood.v5i1.91

Abstract

ABSTRACT Sweet potato is a plant that is easy to be cultivated and very potential to be processed into flour. But the quality is still low compared to other flour especially when heated. Because it is done to improve the quality especially when heated. Modification done crosslink using sodium tripolyphosphate reagent (STPP). The purpose of this study was to determine the effect of modified flour on flour characteristics when heated. Parameters used in this research are water absorption, Swelling Power, and flour solubility. The data processing used is RAL of 1 factor at the 0.05 level and further test using Duncan test. The results showed that the characteristics of flour modification is better than natural flour. The higher the concentration of STPP used, the water absorbency increases, the swelling power increases, and solubility decreases. Sweet potato flour has optimum absorption limit and optimal development on reaction process for 1 hour, and will decrease at longer reaction process.. Key words: sweet potato, sweet potato flour, modified flour, crosslink modification, STPP ABSTRAK Ubi jalar merupakan tanaman yang mudah dibudidayakan dan berpotensi diolah menjadi tepung, namun kualitasnya masih rendah dibandingkan dengan tepung lainnya, khususnya saat dipanaskan. Oleh karena itu, perlu dilakukan upaya meningkatkan kualitas tepung ubi jalar terutama saat dipanaskan. Modifikasi dilakukan secara crosslink dengan pereaksi sodium tripolyfosfat (STPP). Tujuan penelitian ini adalah menentukan pengaruh modifikasi terhadap karakteristik tepung saat dipanaskan. Parameter yang digunakan pada penelitian ini adalah daya serap air, swelling power, dan solubillitas tepung. Pengolahan data menggunakan RAL faktorial pada taraf 0,05 dan uji lanjut menggunakan uji Duncan. Hasil penelitian menunjukan bahwa karateristik tepung modifikasi lebih baik daripada tepung alami. Semakin tinggi konsentrasi STPP, daya serap air meningkat, nilai swelling power meningkat, dan solubilitas menurun. Tepung ubi jalar memiliki batas optimum penyerapan serta pengembangan optimal pada reaksi selama 1 jam dengan konsenrasi STTP 3%, serta menurun pada reaksi yang lebih lama. Kata kunci: ubi jalar, tepung ubi jalar, modifikasi pati, modifikasi crosslink, STPP