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All Journal Journal of Pharmaceutical and Sciences. Borneo Journal of Biology Education (BJBE)
Mastuti Widianingsih
Universitas Malahayati
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health

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Studi In Vitro : Aktivitas Antijamur Kulit Buah dan Daun Coffea canephora Terhadap Candida albicans Mastuti Widianingsih; Yesi Afrida Aini
Journal of Pharmaceutical and Sciences JPS Volume 9 Nomor 2 (2026)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v9i2.1456

Abstract

Candida albicans is a major cause of invasive candidiasis with high morbidity and mortality rates, and it has shown increasing resistance to antifungal agents. The objective of this research to evaluate the antifungal activity of Coffea canephora peel and leaf extract against Candida albicans in vitro by determining the Minimum Inhibitory Concentration (MIC). Robusta coffee peel and leaves were extracted using maceration with 96% ethanol, followed by phytochemical screening and antifungal activity testing using the broth dilution method. Phytochemical screening revealed the presence of alkaloids, phenolic-tannins, flavonoids, saponins, and terpenoids, while steroid were not detected. Based on the antifungal activity test conducted separately, both extracts had the same MIC value at a concentration of 12,5%. Statistical analysis using One Way ANOVA demonstrated a significant difference among treatment groups (p<0,05). The antifungal activity of the extract is presumed to be associated with phenolic compounds, such as chlorogenic acid and caffeine, which may act through disruption of cell membrane integrity and inhibition of ergosterol synthesis. These finding suggest that Coffea canephora peel and leaf extract has the potential to be further developed as an antifungal agent, especially in topical formulations, but further toxicity tests and in vivo tests are required.
Sitotoksisitas Ekstrak Daun Kopi Robusta (Coffea canephora) terhadap Sel Vero Mastuti Widianingsih; M. Rofi Putra Bani; Putri Amalia
Journal of Pharmaceutical and Sciences JPS Volume 9 Nomor 3 (2026)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v9i3.1624

Abstract

Robusta coffee leaves (Coffea canephora) contain various bioactive compounds that may serve as natural antioxidants and potential phytopharmaceutical agents. The objective of this research to evaluate the antioxidant activity and cytotoxicity of the ethanol extract of robusta coffee leaves and to determine the relationship between antioxidant activity and cell viability. Antioxidant activity was assessed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, while cytotoxicity was evaluated on Vero cells using the MTT assay over a concentration range of 31.25-500 µg/mL. Vitamin C and doxorubicin were used as positive controls. The ethanol extract exhibited very strong antioxidant activity with an IC50 value of 22.456 µg/mL, while vitamin C showed an IC50 value of 4.217 µg/mL. Cytotoxicity testing demonstrated low toxicity toward Vero cells, with an IC50 value of 1512.857 µg/mL, whereas doxorubicin exhibited an IC50 value of 60.052 µg/mL. Pearson correlation analysis revealed a strong negative correlation between antioxidant activity and Vero cell viability (r = −0.93, p = 0.024). Despite this correlation, cell viability remained above 100% at all tested concentrations, indicating the absence of cytotoxic effects on normal mammalian cells. These findings suggest that the ethanol extract of robusta coffee leaves possesses potent antioxidant activity while maintaining a favorable safety profile, supporting its potential to be futher development as a source of  natural antioxidants and phytopharmaceutical candidate.
Daun dan Kulit Buah Kopi Robusta (Coffea canephora Pierre ex A.Froehner) Sebagai Sumber Senyawa Bioaktif untuk Aplikasi Farmakologi Mastuti Widianingsih
Borneo Journal of Biology Education (BJBE) Vol. 7 No. 2 (2025): Oktober
Publisher : Department of Biology Education, Universitas Borneo Tarakan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35334/fp1sg371

Abstract

Kopi menjadi salah satu komoditas perkebunan strategis dengan nilai ekonomi tinggi dan distribusi konsumsi yang luas secara global. Selain biji kopi yang menjadi produk utama, bagian tanaman lain seperti daun dan kulit buah kopi diketahui mengandung beragam senyawa metabolit sekunder yang berpotensi memberikan manfaat farmakologis. Penelitian ini bertujuan untuk mengidentifikasi kandungan metabolit sekunder pada ekstrak daun dan kulit buah kopi robusta melalui uji fitokimia kualitatif. Sampel diambil dari perkebunan kopi di Lampung Barat, diidentifikasi secara morfologi, kemudian diekstraksi dan dianalisis menggunakan pereaksi spesifik untuk mendeteksi alkaloid, flavonoid, fenolik/tanin, saponin, terpenoid, dan steroid. Hasil analisis menunjukkan bahwa ekstrak daun maupun kulit buah kopi robusta positif mengandung alkaloid (presipitasi putih-cokelat), flavonoid (warna kuning kecokelatan disertai busa), fenolik/tanin (warna hijau kebiruan), saponin (buih stabil ±30 detik), dan terpenoid (cincin merah), sedangkan senyawa golongan steroid tidak terdeteksi. Keberadaan senyawa tersebut mengindikasikan potensi aktivitas biologis seperti antioksidan, antiinflamasi, antimikroba, dan kardioprotektif, sebagaimana dilaporkan dalam penelitian terdahulu dalam satu dekade terakhir. Temuan ini memperkuat prospek pemanfaatan bagian non-biji kopi robusta sebagai sumber senyawa bioaktif yang dapat dikembangkan dalam industri fitofarmaka dan pangan fungsional berbasis tanaman.
Co-Authors M. Rofi Putra Bani Putri Amalia Yesi Afrida Aini