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All Journal Jurnal Bioleuser
Lenni Fitri
Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Syiah Kuala, Aceh, Indonesia

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Toksisitas Aktinobakteri ATS-7 Asal Tanah Stasiun Penelitian Soraya Kawasan Ekosistem Leuser Berdasarkan Waktu dan Suhu Inkubasi Dhea Amanda Adryan; Lenni Fitri; Fauziah Fauziah
Jurnal Bioleuser Vol. 9 No. 3: Desember 2025
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jurnalbioleuser.v9i3.1430

Abstract

Actinobacteria are prokaryotic microorganisms that have a morphological form resembling filamentous fungi, and are generally found in soil and have many beneficial potentials. This study aims to determine the effect of the toxicity test of actinobacteria ATS-7 from the soil of the Soraya Research Station based on incubation time and temperature. Toxicity testing was carried out using the Brine Shrimp Lethality Test (BSLT) method to obtain the LC₅₀ value. The toxicity test was carried out by calculating the number of dead Artemia salina larvae. The results of the toxicity test showed that the ATS-7 isolate at a temperature of 32ºC which was shaken for 5 days with an LC₅₀ value of 77.49 ppm, 10 days with an LC₅₀ value of 62.88 ppm, 15 days with an LC₅₀ value of 53.70 ppm. This temperature has very high toxicity activity, and has an LC₅₀ value of <100 ppm, meaning that the incubation time at 32ºC greatly affects the toxicity test and is very toxic.
Pertumbuhan Kapang Aspergillus niger dan Penicillium sp. yang Diinokulasikan pada Buah Stroberi (Fragaria sp.) (Isolation and Analysis of Proteolytic Activity of Local Actinomycetes from Aceh, Indonesia) Lenni Fitri; Aini Syafitri; Mirryazil Jannah; Anasya Khansa Yumna; Lilis Fitria; Ira Maya Wahyuni
Jurnal Bioleuser Vol. 9 No. 1: April 2025
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jurnalbioleuser.v9i1.1794

Abstract

Strawberries are susceptible to attack by pathogenic fungi which can cause damage and affect the quality of strawberries. This research aims to determine the growth time of the Mould Penicillium sp. and Aspergillus niger which were inoculated on strawberries and determine the level of damage caused by the Mould Penicillium sp. and Aspergillus niger on strawberries. This research was carried out by inoculating the Mould on Penicillium sp. and Aspergillus niger on strawberries, observe the growth of these two fungi and determine the level of damage caused to strawberries due to the Mould. The results of this research show that the Penicillium sp. Mould grows faster on strawberries, starting on the third day. Meanwhile, the Aspergillus niger Mould was seen starting to grow on the fourth day of incubation. The level of damage to strawberries on day 7 was rotting and the presence of liquid produced.
Antifungal Activity of Lactic Acid Bacteria as A Biopreservation in Sourdough Production Lenni Fitri; Sinta Ayu Mauludin; Sarah Annisa Ananda Putri; Indry Malliny; Nanda Safira; Rahadatul Aisy; Raudhatul Jannah; Zulia Ananda; Dalil Sutekad
Jurnal Bioleuser Vol. 8 No. 2: Agustus 2024
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jurnalbioleuser.v8i2.1945

Abstract

Bread is a food made from flour and yeast. Bread made using conventional yeast only has limited shelf life because it is susceptible to the growth of fungi such as Aspergillus niger and Rhizopus stolonifer. One way to increase the shelf life of bread is to use Lactic Acid Bacteria (LAB) as a starter for making bread. This research aims to determine the ability of LAB yakult and yoghurt as an antifungal and to test the organoleptic and shelf life of sourdough with LAB formulation. The method used in this research is an experimental methode. The parameters observed were the antifungal activity of yakult and yoghurt with LAB formulation, organoleptic test of sourdough with LAB formulation, and shelf life test of sourdough with LAB formulation. The result showed that yoghurt LAB had antifungal activity, while yakult LAB did not have antifungal activity. Based on organoleptic test, sourdough with the additional of LAB yoghurt has better organoleptic test result compared to white bread without the additional of LAB yoghurt, and sourdough with the addition of LAB yoghurt has a longer shelf life compared to white bread without the additional of LAB yoghurt.
Kemampuan Daya Hambat Ekstrak Buah Pala (Myristica fragrans H.) Terhadap Candida albicans (Isolation and Analysis of Proteolytic Activity of Local Actinomycetes from Aceh, Indonesia) Yulia Sari Ismail; Ainal Haniyati Ulfa; Isnaini Putri; Lenni Fitri
Jurnal Bioleuser Vol. 8 No. 1: April 2024
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jurnalbioleuser.v8i1.1969

Abstract

The aim of this study were to determine the content of phytochemical compounds from tree parts of nutmeg (flesh, mace and seed),to compare the inhibitory effect of the extracts from the tree parts of the nutmeg (flesh, mace and seed) and the concentration that were effective in inhibiting the growth of the fungus Candida albicans. The extraction method was the maceration, the phytochemical testing was carried out qualitatively, and the antifungal activity was tested using punch hole method with concentrations of 25, 50, 75 and 100 %, positive control using ketoconazole and negative control using steril distilled water The result of this study showed that the etanol extract of nutmeg flesh was effective in inhibiting the growth of the fungus Candida albicans at concentration 75 % dan 100%. While the seed extract of nutmeg had a low inhibition zone. The etanol extract of nutmeg flesh and mace can be potential as antifungal.