Susilaningsih, Dwi -
LIPI Press

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SUBSTITUTION COMPONENT CULTIVATION MEDIA OF PHOTO-FERMENTATION PROCESS FOR LOW COST HYDROGEN GAS BIOPRODUCTION Susilaningsih, Dwi -; Uhtiza, Tami Astie; Anam, Khairul -
Teknologi Indonesia Vol 40, No 2 (2017)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (359.597 KB) | DOI: 10.14203/jti.v40i2.378

Abstract

Employing the Rhodobium marinum bacteria as an agent of hydrogen bioproduction with facultative fermentation condition needs two steps of cultivations, i.e., pre-culture and hydrogen production periods with different media.  The two steps cultivation is important state for hydrogen gas production rate. ASY (Ammonium, Succinate, and Yeast extract) is usually used as standard medium in biohydrogen production and mostly the component is analytical grade of chemicals. Series of cultivation media substitution by combining ASY with other cost-effective chemicals were investigated. The total of sixteen combinations of media were observed (P0-P15). Cultivation condition of R. marinum was set in an illuminated light of 31.17 µmol s-1m-2photon and agitated in shaker of 120 rpm for nine days. The experiment was designed as random selection with anova analyses. The results exhibited that P7 medium combination released the highest hydrogen gas of 61.29±0.52 ml/80 ml media among the others combination media. Using one-way ANOVA, there was a statistically significant difference between the mean number and condition and it was found P7 was significantly different with the others combination media, except P7 was insignificantly different with P3 only. Overall, the combination media is cheaper than the ASY medium. The ASY medium price is about USD 677, and the substitution media prices were ranging from USD 4.24 to USD 613.47. However, the further research on optimisation cultivation condition is needed.
LACTIC ACID AND HYDROGEN PRODUCTION FROM MICROALGAL BLOOM BIOMASSES Susilaningsih, Dwi -; Harahap, Puspita Sari; Farida, Hilda -; Rahman, Delicia Yunita
Teknologi Indonesia Vol 40, No 1 (2017)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (16.054 KB)

Abstract

Microalgal bloom occurrence is rapidly increasing in a very short time in a specific condition and dominating its habitat. Therefore, blooming often occurs in water ecosystems, from freshwater to brackish and marine. Although there is no officially acknowledged threshold concentration level, the amount of algae cells is estimated reaching hundreds or even thousands cells per mililiter, depending on the species and types. Algae blooming is considered harmful because it gives such a big impact varies on water ecosystems, depending on the species involved, the environment where they are found, and their mechanism metabolism. The hazardous algae poison causes severe effect to various species, such as mammals and other sea creatures, with each presenting specific toxicity in decreasing motor development, immunology, neurological capacity, and reproduction capacity.In this report, the tropical algal bloom biomasses were used for fermentation substrate as substituent of carbon sources in purpose to produce a useful compound of lactic acid. Lactic acid is base material used in forming polylactic acid (PLA) polymerase that is known as basic material for biohydrogen precursor. The lactate bacteria were isolated from local tropical fruit.The result showed all microalgal biomasses converted successfully into lactic acid for 68–92% based on total carbohydrates conversion into lactic acid compounds. Algal bloom biomasses contained mostly of carbohydrate complexes (60–70% of dry weight), protein (10–20% of dry weight), lipid (20–30% of dry weight), and other compounds (5–10% of dry weight). The fermentation result showed that various microalgal blooms biomasses were successfully converted into lactic acid ranging from 58–92% based on total carbohydrates conversion into lactates. Furthermore, the obtained lactic acid was converted into polylactic acid through direct polycondensation process with average result of 60–84% formation unit base on lactic acid weight. Therefore, lactic acid was also successfully converted into hydrogen gas using fermentation process with efficient value from 78–90% unit based on the weight of lactic acid molecules. All these results showed that the unuseful algal biomass could be utilized as a substrate or precursor of fermentation process in term of biodegradable material and energy production, and also lactic acid is an applicable compound in forming polymers and energy.