Puspita Sari Harahap
Program Studi Ilmu Kelautan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Dipenogoro

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LACTIC ACID AND HYDROGEN PRODUCTION FROM MICROALGAL BLOOM BIOMASSES Susilaningsih, Dwi -; Harahap, Puspita Sari; Farida, Hilda -; Rahman, Delicia Yunita
Teknologi Indonesia Vol 40, No 1 (2017)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (16.054 KB)

Abstract

Microalgal bloom occurrence is rapidly increasing in a very short time in a specific condition and dominating its habitat. Therefore, blooming often occurs in water ecosystems, from freshwater to brackish and marine. Although there is no officially acknowledged threshold concentration level, the amount of algae cells is estimated reaching hundreds or even thousands cells per mililiter, depending on the species and types. Algae blooming is considered harmful because it gives such a big impact varies on water ecosystems, depending on the species involved, the environment where they are found, and their mechanism metabolism. The hazardous algae poison causes severe effect to various species, such as mammals and other sea creatures, with each presenting specific toxicity in decreasing motor development, immunology, neurological capacity, and reproduction capacity.In this report, the tropical algal bloom biomasses were used for fermentation substrate as substituent of carbon sources in purpose to produce a useful compound of lactic acid. Lactic acid is base material used in forming polylactic acid (PLA) polymerase that is known as basic material for biohydrogen precursor. The lactate bacteria were isolated from local tropical fruit.The result showed all microalgal biomasses converted successfully into lactic acid for 68–92% based on total carbohydrates conversion into lactic acid compounds. Algal bloom biomasses contained mostly of carbohydrate complexes (60–70% of dry weight), protein (10–20% of dry weight), lipid (20–30% of dry weight), and other compounds (5–10% of dry weight). The fermentation result showed that various microalgal blooms biomasses were successfully converted into lactic acid ranging from 58–92% based on total carbohydrates conversion into lactates. Furthermore, the obtained lactic acid was converted into polylactic acid through direct polycondensation process with average result of 60–84% formation unit base on lactic acid weight. Therefore, lactic acid was also successfully converted into hydrogen gas using fermentation process with efficient value from 78–90% unit based on the weight of lactic acid molecules. All these results showed that the unuseful algal biomass could be utilized as a substrate or precursor of fermentation process in term of biodegradable material and energy production, and also lactic acid is an applicable compound in forming polymers and energy.
Pengaruh Substitusi Limbah Cair Tahu Untuk Menstimulasi Pembentukan Lipida Pada Chlorella sp. Harahap, Puspita Sari; Susanto, Antonius Budi; Susilaningsih, Dwi; Rahma, Delicia Yunita
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.672 KB) | DOI: 10.14710/jmr.v2i1.2070

Abstract

Chlorella sp. is one of microalgae which has potential lipid content. Chlorella sp. has a rapid growth rate and also easy to maintain. Therefore, chlorella has potency as source in biodiesel production.This aim of study is to determine substitution of carbon source as regular medium with tofu liquid waste to enhanced lipid production of Chlorella sp. This research was conducted by laboratory experimental method using Completely Randomized Design (CRD) with six treatments (three replicates) and one control. Material in this research is Chlorella sp. that obtained from Puslit Bioteknologi- Bioproses division, Lembaga Ilmu Pengetahuan Indonesia, Cibinong. Research’s data is analyzed using analysis variant (for the normal and homogenous data) or using non-parametric (for the abnormal and in homogenous data). The result of this study indicates treatment which has higher lipid is Chlorella sp.’s cultivation with tofu liquid waste addition 15 % in day 42nd (0,5160 g/ L), and treatment with tofu liquid waste addition 20 % in day 45th has the highest oil content (0,4040 g/ L).Culture of Chlorella sp. with tofu liquid waste as medium resulted decrese lipid production.