Purpose - This study aims to develop a spatio-temporal load characterization framework for electrified two-wheeler mobility and evaluate the potential implications of charging demand on urban distribution networks under varying operational scenarios. Design/methodology/approach - The framework integrates mobility behavior, energy consumption, and charging demand using key parameters, including travel distance (40–80 km/day), energy consumption rate (0.04–0.06 kWh/km), and fleet size (100–1000 units). Findings - Results show that individual energy demand ranges from 1.6 to 4.8 kWh/day per vehicle, while aggregated demand increases proportionally, reaching approximately 0.16 MWh/day, 0.80 MWh/day, and 1.60 MWh/day for fleets of 100, 500, and 1000 units, respectively. Charging demand is highly concentrated during evening periods, creating synchronized load peaks and localized stress on distribution infrastructure. Research implications/limitations - The study is limited to simulated mobility and charging scenarios; however, it provides a practical framework for assessing the impacts of electric two-wheeler adoption on urban power distribution systems. Originality/value - This research offers an integrated spatio-temporal approach combining mobility patterns, energy consumption, and charging behavior within a unified framework, providing valuable insights for coordinated charging strategies, load balancing, and infrastructure planning.