Article Per Year (5 Year)
p-Index From 2021 - 2026
0.751
P-Index
This Author published in this journals
All Journal International Journal of Health, Economics, and Social Sciences (IJHESS) Jurnal Kolaboratif Sains
Wiwit Zuriati Uno
Dosen program studi S1 Farmasi UNG
Religion Agriculture, Biological Sciences & Forestry Humanities Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Health Professions Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Other

Published : 4 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 4 Documents
Search

 1 
Isolation and Characterization of L-Asparaginase Producing Endophytic Fungi from Plants of the Amaryllidaceae Family Asri Setiani Kadir; A. Mu’thi Andy Suryadi; Mahdalena Sy. Pakaya; Faramita Hiola; Wiwit Zuriati Uno
International Journal of Health, Economics, and Social Sciences (IJHESS) (Special Issue) - International Journal of Health, Economics, and Social Sciences (IJHESS) - July 20
Publisher : Universitas Muhammadiyah Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56338/ijhess.v8i3.11124

Abstract

L-asparaginase is an enzyme that catalyzes the conversion of L-asparagine into L-aspartate and inhibits the growth of leukemia cells that depend on asparagine. This study aims to isolate and characterize endophytic fungi from plants of the Amaryllidaceae family, and to evaluate the ability of these isolates to produce the L-asparaginase enzyme. This study employed an experimental method conducted in a pharmaceutical microbiology laboratory. Endophytic fungi were isolated using surface sterilization techniques, followed by cultivation on Potato Dextrose Agar (PDA) media. Enzyme production was performed in liquid fermentation, whereas enzyme purification was conducted using ammonium sulfate fractionation with concentrations of 0–20%, 20–40%, 40–60%, 60–80%, and 80–100%. The activity of L-asparaginase enzyme was tested using the Nesslerization method, with Asparagus used as an inducer. The findings showed that several endophytic fungal isolates produced L-asparaginase, as indicated by a yellowish-brown color in the Nessler test. The purified fractions exhibited varying enzyme activities at different levels of ammonium sulfate saturation. Therefore, endophytic fungi from plants of the Amaryllidaceae family have potential as alternative sources of the L-asparaginase enzyme, which can be further developed in pharmacy and biotechnology.
Perbandingan Produksi Enzim Selulase Dari Aspergillus niger Dengan dan Tanpa Menggunakan Penginduksi Carboxymethyl Cellulose (CMC): Comparison of Cellulase Enzyme Production from Aspergillus niger With and Without Using Carboxymethyl Cellulose (CMC) Inducer Aldevi Trisnawati Adam; Mahdalena Sy. Pakaya; Faramita Hiola; La Ode Aman; Wiwit Zuriati Uno
Jurnal Kolaboratif Sains Vol. 8 No. 9: September 2025 - Jurnal Kolaboratif Sains (JKS)
Publisher : Universitas Muhammadiyah Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56338/jks.v8i9.8536

Abstract

Enzim selulase merupakan enzim yang mampu mendegradasi selulosa dengan produk utamanya yakni glukosa, selobiosa dan xilooligosakarida, selulase memiliki sistem enzim yang tersusun dari endo-1,4-?-glukanase, ekso-1,4-?-glukanase dan ?-D-glukosidase. Penelitian ini bertujuan untuk memproduksi enzim selulase dari Aspergillus niger dengan menggunakan penginduksi berupa CMC. Proses produksi dilakukan dengan menggunakan metode fermentasi cair dengan dan tanpa penambahan inducer. Aktivitas enzim selulase diuji secara kualitatif dilihat berdasarkan pembentukan zona hidrolitik. Hasil penelitian menunjukan bahwa enzim selulase yang diproduksi menggunakan inducer CMC membentuk zona hidrolitik lebih besar dibandingkan dengan tidak menggunakan inducer dengan nilai indeks aktivitas enzim secara berturut-turut sebesar 2,5 mm dan 1,93 mm. Dapat disimpulkan bahwa Aspergillus niger dapat menghasilkan enzim selulase lebih banyak dengan penambahan inducer CMC dibandingkan dengan tidak menggunakan inducer
Isolation and Characterization of Endophytic Fungi Producing L-Asparaginase Enzyme from Kencur Plant (Kaempferia galanga L.) Riyanti Djafar; Mahdalena Sy. Pakaya; A.Mu’thi Andy Suryadi; Mohamad Aprianto Paneo; Wiwit Zuriati Uno
International Journal of Health, Economics, and Social Sciences (IJHESS) (Special Issue) - International Journal of Health, Economics, and Social Sciences (IJHESS) April 202
Publisher : Universitas Muhammadiyah Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56338/ijhess.v8i2.10927

Abstract

L-Asparaginase is an enzyme that catalyzes the hydrolysis of L-asparagine into L-aspartate and ammonia. This enzysne has important benefits in the medical field, particularly as a biological agent is the therapy of acute lymphoblastic leukemia, due to its ability to inhibit the growth of asparagine-dependent cancer cells. This study aims to isolate and characterize endophytic fungi derived from the rhizome of the aromatic ginger (Kaempferia galanga L.) plant, and to test the ability of these isolates to produce the L-asparaginase enzyme. The research method used is an experimental method conducted in a pharmaceutical microbiology laboratory Endophytic fungi were isolated using surface sterilization techniques and culturing on PDA media. Enzyme production was performed using the submerged fermentation method, while enzyme purification used the ammonium sulfate fractionation method with concentration ranges of 0-20%, 20-40%, 40-60%, 60-80%, and 80-100%. The L-asparaginase enzyme activity test was conducted using the Nesslerization method, with Asparagus as an inducer. The results showed that several endophytic fungal isolates were capable of producing L-asparaginase, as evidenced by a brownish color in the Nessler test. The purified fractions showed varying enzyme activity at each level of ammonium sulfate saturation. Thus,endophytic fungi from the aromatic ginger rhizome have potential as an alternative source for producing the L-asparaginase enzyme, which can be further developed in the fields of pharmacy and biotechnology
Efektivitas Metabolit Sekunder Jamur Endofit Tanaman Lamun (Thalassia hemprichii) Terhadap Biofilm Bakteri Yang Diisolasi Dari Nasogastric Tube: The Effectiveness of Secondary Metabolites of Endophytic Fungi of Seagrass Plants (Thalassia hemprichii) Against Bacterial Biofilms Isolated from Nasogastric Tubes Fadlun Ladiku; Mahdalena Sy. Pakaya; Endah Nurrohwinta Djuwarno; Ariani H Hutuba; Wiwit Zuriati Uno
Jurnal Kolaboratif Sains (Special Issue) - Jurnal Kolaboratif Sains (JKS) - Januari 2026
Publisher : Universitas Muhammadiyah Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56338/jks.v9i1.9748

Abstract

Biofilm merupakan komunitas mikroorganisme yang melekat kuat pada permukaan dan dilindungi oleh matriks ekstraseluler (EPS), yang menyebabkan meningkatnya resistensi terhadap antibiotik dan desinfektan. Pembentukan biofilm pada alat medis seperti Nasogastric Tube (NGT) menjadi salah satu penyebab utama infeksi nosokomial. Penelitian ini bertujuan untuk mengetahui aktivitas antibiofilm metabolit sekunder jamur endofit lamun Thalassia hemprichii terhadap bakteri pembentuk biofilm yang diisolasi dari NGT. Metode yang digunakan meliputi uji pencegahan perlekatan biofilm, penghambatan pertumbuhan biofilm, dan penghancuran (degradasi) biofilm, dengan media BHIB dan BHIB + 1% sukrosa menggunakan metode tabung yang diwarnai dengan kristal violet 1%. Hasil penelitian menunjukkan bahwa metabolit sekunder jamur endofit T. hemprichii mampu menurunkan ketebalan biofilm secara signifikan, ditunjukkan oleh meningkatnya nilai Mean Gray Value (MGV) pada ketiga uji aktivitas antibiofilm. Nilai MGV tertinggi diperoleh pada media BHIB + 1% sukrosa dengan isolat bakteri pasien A, yaitu 154,05 ± 4,20 pada uji pencegahan perlekatan, 145,14 ± 2,58 pada uji penghambatan pertumbuhan, dan 156,84 ± 9,05 pada uji penghancuran biofilm. Aktivitas antibiofilm ini diduga disebabkan oleh kandungan senyawa metabolit sekunder seperti flavonoid, fenolik, alkaloid, dan terpenoid yang bekerja melalui mekanisme penghambatan sistem quorum sensing, penekanan produksi EPS, serta gangguan adhesi dan integritas sel bakteri. Dengan demikian, metabolit sekunder jamur endofit Thalassia hemprichii berpotensi dikembangkan sebagai agen antibiofilm alami untuk mencegah pembentukan biofilm pada alat medis seperti NGT dan mengurangi risiko infeksi nosokomial.
Co-Authors A. Mu’thi Andy Suryadi A.Mu’thi Andy Suryadi Aldevi Trisnawati Adam Ariani H Hutuba Asri Setiani Kadir Endah Nurrohwinta Djuwarno Fadlun Ladiku Faramita Hiola La Ode Aman Mahdalena Sy. Pakaya Mohamad Aprianto Paneo Riyanti Djafar