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All Journal Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Sri Helianty
Universitas Riau, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions

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HYDROLYTIC ENZYME SYSTEMS OF Trichoderma spp.: FERMENTATION STRATEGIES, STRAIN ENGINEERING, AND SUSTAINABLE LIGNOCELLULOSIC VALORIZATION Sri Helianty; Yuana Nurulita; Panca Setia Utama; Titania Tjandrawati Nugroho
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Hydrolytic enzymes produced by Trichoderma spp. play a central role in lignocellulosic biomass conversion due to their high secretion capacity and catalytic versatility. This review provides a critical and integrated analyzes of enzyme systems, fermentation strategies, and bioprocess engineering approaches governing enzyme production and biomass valorization. A structured literature review was conducted using peer-reviewed publication 2019 to 2025. The analysis highlights synergistic interactions among cellulases, hemicellulases, and oxidative enzymes in overcoming biomass recalcitrance. Key process parameters—including aeration, moisture content, temperature regulation, and bioreactor design—are identified as a primary deteminants of productivity and efficiency. Solid-state fermentation is recognized as an efficient strategy for valorizing agricultural residues such as rice straw, although challenges in heat and mass transfer and scale-up remain. Advances in strain engineering, regulatory pathway optimization, and enzyme cocktail design improve hydrolytic performance, but trade-offs between productivity, stability, and process robustness persist. Integration within circular biorefinery frameworks is essential for scalable and sustainable biomass conversion.
HYDROLYTIC ENZYME SYSTEMS OF Trichoderma spp.: FERMENTATION STRATEGIES, STRAIN ENGINEERING, AND SUSTAINABLE LIGNOCELLULOSIC VALORIZATION Sri Helianty; Yuana Nurulita; Panca Setia Utama; Titania Tjandrawati Nugroho
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Hydrolytic enzymes produced by Trichoderma spp. play a central role in lignocellulosic biomass conversion due to their high secretion capacity and catalytic versatility. This review provides a critical and integrated analyzes of enzyme systems, fermentation strategies, and bioprocess engineering approaches governing enzyme production and biomass valorization. A structured literature review was conducted using peer-reviewed publication 2019 to 2025. The analysis highlights synergistic interactions among cellulases, hemicellulases, and oxidative enzymes in overcoming biomass recalcitrance. Key process parameters—including aeration, moisture content, temperature regulation, and bioreactor design—are identified as a primary deteminants of productivity and efficiency. Solid-state fermentation is recognized as an efficient strategy for valorizing agricultural residues such as rice straw, although challenges in heat and mass transfer and scale-up remain. Advances in strain engineering, regulatory pathway optimization, and enzyme cocktail design improve hydrolytic performance, but trade-offs between productivity, stability, and process robustness persist. Integration within circular biorefinery frameworks is essential for scalable and sustainable biomass conversion.
Co-Authors Panca Setia Utama Titania Tjandrawati Nugroho Yuana Nurulita