Putu Angga Wiradana
Study Program of Biology, Faculty of Health and Science, Universitas Dhyana Pura, Badung, Bali, Indonesia

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Field Testing of Multispecies Probiotics on Health Status, Water Quality, and Productivity of Pacific White Shrimp (Litopenaeus vannamei) Lindah Erlia Safira Siregar; Sinung Rahardjo; Tb. Haeru Rahayu; Putu Angga Wiradana
Jurnal Perikanan UGM (Journal of Fisheries Sciences) Vol 28, No 1 (2026)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.111975

Abstract

Shrimp farming has rapidly grown into a significant sector worldwide, providing economic benefits and high-quality food. Probiotics are widely used in shrimp farming to enhance growth and prevent diseases. However, reports on the field-scale application of multispecies probiotics in shrimp farming are minimal. This study aims to evaluate the effectiveness of multispecies probiotics on the health status, production performance, and water quality of L. vannamei shrimp farming at a field scale. L. vannamei post-larvae were reared in maintenance ponds. The shrimp seeds were stocked in 10 plots (50×50×2 m²) and acclimated for 7 days. Multispecies probiotics were administered after acclimatization, and health status parameters, production performance, and water quality were subsequently measured. The results indicated that multispecies probiotics combined with molasses and fermented bran were ineffective at preventing V. parahaemolyticus growth, as evidenced by the health status of L. vannamei. However, multispecies probiotics improved production performance and water quality through the end of the maintenance period. This study highlights the effectiveness of multispecies probiotics in enhancing production performance and water quality. Further research is needed to understand the mechanism of action of these probiotics in vannamei shrimp at a field scale.
Phenotype and genotype detection of Escherichia coli and Salmonella spp. contamination in fishery commodities in Bali, Indonesia Aris Jatmiko; Ni Kadek Dwipayani Lestari; Anak Agung Ayu Putri Permatasari; Putu Eka Sudaryatma; Pascal Sebastian; Muhammad Khaliim Jati Kusala; Putu Angga Wiradana
Jurnal Medik Veteriner Vol. 9 No. 2 (2026): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol9.iss2.2026.368-382

Abstract

This study aimed to identify Salmonella spp. and Escherichia coli contamination associated with foodborne diseases in fishery commodities from Bali, Indonesia. A total of 216 samples representing commercially important fish and shrimp species were collected from suppliers, fish processing units, micro, small, and medium enterprises (MSMEs), traditional markets, and modern markets across regencies in Bali. Sampling was proportionally distributed across supply chain loci, with three replicate samples collected for each commodity at each locus to minimize sampling bias and improve prevalence estimates. Phenotypic detection was performed using selective culture media, while positive isolates were confirmed genotypically using specific primers targeting the invA gene for Salmonella spp. and the lacZ gene for E. coli. The results revealed the presence of E. coli contamination in all fishery commodities examined, albeit with varying prevalence across commodity types. Conversely, Salmonella spp. was not detected in any of the samples. The highest prevalence of E. coli contamination by sampling locus was observed in traditional markets (12/36; 33%), whereas lemuru fish exhibited the highest prevalence among commodity types (5/10; 50%). Spatially, Tabanan Regency showed the highest prevalence of E. coli contamination, reaching up to 66% across the surveyed villages. Genotypic confirmation successfully identified E. coli isolates based on amplification of the lacZ gene at 876 bp. These findings highlight the need to implement a One Health approach as an effective framework for controlling foodborne bacterial contamination and its environmental transmission.