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All Journal Journal of Stem Cell Research and Tissue Engineering
Wasis Syahwanda
Dental Engineering Study Program, Hang Tuah School of Health Sciences
Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary

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THE EFFECTIVENESS OF STEM CELL SECRETOME ON POST-TOOTH EXTRACTION WOUND HEALING Depi Lusianti; Wasis Syahwanda
Journal of Stem Cell Research and Tissue Engineering Vol. 10 No. 1 (2026): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v10i1.92273

Abstract

Tooth extraction is a common surgical procedure performed in dental practice and may cause damage to both soft tissue and alveolar bone. The post-tooth extraction wound healing process involves various biological mechanisms, including inflammation, cell proliferation, angiogenesis, and tissue remodeling. In recent years, stem cell secretome has emerged as a promising regenerative therapy approach because it contains various bioactive factors such as growth factors, cytokines, and extracellular vesicles that play important roles in tissue repair. This study aimed to evaluate the effectiveness of stem cell secretome on post-tooth extraction wound healing through a systematic review method. Literature searching was conducted using the PubMed, Google Scholar, and ScienceDirect databases within the 2020–2026 publication period. Articles were selected based on inclusion and exclusion criteria according to the PRISMA guidelines. The review results showed that stem cell secretome was able to enhance fibroblast proliferation, angiogenesis, collagen deposition, wound epithelialization, and alveolar bone regeneration. In addition, secretome also played a role in reducing inflammatory responses, thereby accelerating the oral wound healing process. Based on the results of this systematic review, stem cell secretome has effective potential as a regenerative therapy to accelerate post-tooth extraction wound healing. However, further clinical studies are still needed to confirm its effectiveness and safety in humans.
IDENTIFICATION OF KEY GENES AND MOLECULAR PATHWAYS ASSOCIATED WITH OSTEOGENIC DIFFERENTIATION OF DENTAL PULP STEM CELLS Wasis Syahwanda; Elsa Fitri
Journal of Stem Cell Research and Tissue Engineering Vol. 10 No. 1 (2026): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v10i1.92286

Abstract

Dental pulp stem cells (DPSCs) have emerged as promising candidates for regenerative dentistry due to their self-renewal capacity and multilineage differentiation potential, particularly in osteogenic differentiation. Understanding the molecular mechanisms underlying this process is essential for developing effective regenerative therapies. This study aimed to identify key genes and molecular pathways involved in the osteogenic differentiation of DPSCs using a bioinformatics-based in silico approach. Gene expression data were obtained from the Gene Expression Omnibus (GEO) database, specifically the GSE80614 dataset, which compared undifferentiated and osteogenically induced human DPSCs. Differentially expressed genes (DEGs) were identified using GEO2R with the criteria of p-value < 0.05 and |log fold change| ≥ 1. Functional enrichment analysis was conducted using DAVID, while protein–protein interaction (PPI) networks were analyzed using STRING and Cytoscape. The analysis revealed distinct transcriptional differences between undifferentiated and osteogenically differentiated DPSCs. Functional enrichment demonstrated significant involvement of biological processes related to extracellular matrix organization, ossification, and regulation of cell differentiation. Key signaling pathways identified included PI3K-Akt signaling, TGF-β signaling, ECM–receptor interaction, and focal adhesion pathways, all of which are closely associated with osteogenesis. PPI network analysis identified several hub genes, including RUNX2, ALPL, BMP2, and COL1A1, suggesting their central regulatory roles in osteogenic differentiation. Overall, this study provides a systematic overview of the molecular mechanisms associated with DPSC osteogenesis and highlights potential therapeutic targets for regenerative dental applications. Further experimental validation is required to confirm these findings.
Co-Authors Depi Lusianti Elsa Fitri