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In Vitro Evaluation of Bacillus subtilis and Pseudomonas fluorescens Against Klebsiella variicola in Kepok Banana Graciella Luise Laoth; Gusti Ngurah Alit Susanta Wirya; I Gede Rai Maya Temaja
Research Horizon Vol. 6 No. 2 (2026): Research Horizon - April 2026
Publisher : LifeSciFi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54518/rh.6.2.2026.1076

Abstract

Bacterial wilt represents a major constraint in the cultivation of Kepok banana (Musa paradisiaca L.) in East Nusa Tenggara, and recent identification of Klebsiella variicola as the causal agent indicates a potential emerging threat to production sustainability. This study aimed to evaluate the antagonistic potential of Bacillus subtilis and Pseudomonas fluorescens against K. variicola under in vitro conditions. The experiment was arranged in a Completely Randomized Design with five treatments, including control groups, single antagonistic bacteria, and their combination, using a dual culture assay with the paper disc method. The results demonstrated that Bacillus subtilis exhibited the highest inhibitory activity, followed by the combination treatment and Pseudomonas fluorescens, while no inhibition was observed in the negative control. The superior performance of Bacillus subtilis is associated with its ability to produce antibacterial metabolites, particularly lipopeptides that disrupt pathogen cell structures. In contrast, Pseudomonas fluorescens inhibited pathogen growth through siderophores and toxic compounds, although with lower effectiveness. The combination treatment did not produce a synergistic effect, likely due to microbial competition. These findings suggest that Bacillus subtilis holds strong potential as an environmentally friendly biological control agent. Further in vivo studies are required to validate its effectiveness under field conditions.
Pengaruh Pemberian Paket Pupuk terhadap Pertumbuhan dan Perubahan Sifat Kimia Tanah pada Tanaman Jeruk Keprok Tejakula Ni Made Trigunasih; I Nyoman Rai; I Nyoman Wijaya; Gede Wijana; Gusti Ngurah Alit Susanta Wirya; Widhianthini Widhianthini; Ni Nyoman Ari Mayadewi; I Kadek Wisma Yudha; I Wayan Eka Karya Utama; I Wayan Surya Aditya Wigunanda
Bali Membangun Bali: Jurnal Bappeda Litbang Vol 6 No 2 (2025): Agustus 2025
Publisher : Badan Riset dan Inovasi Daerah Provinsi Bali

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51172/jbmb.v6i2.467

Abstract

Purpose: This research aims to analyze the effect of organic and inorganic fertilizer packages on the growth of Tejakula tangerine plants and changes in soil chemical properties after being given organic and inorganic fertilizer packages. Research methods: The method used in this research was a Randomized Block Design (RAK) with 1 (one) factor. The treatment tested was the fertilizer package dose (P), consisting of 3 levels, namely organic fertilizer/cow manure (Po), organic cow manure + 22.6 g Urea fertilizer + 4 g KCl fertilizer + Phonska Plus 15 fertilizer -15-15 dose 84 g (Ph), and organic cow manure fertilizer + Urea fertilizer dose 22.6 g, KCl fertilizer dose 4 g, Phonska Plus fertilizer 15-15-15 dose 84 g + mycorrhizal biofertilizer + liquid organic fertilizer (POC) probiotics (Pc). Each treatment was repeated 8 times to obtain 24 treatments. Findings: The response to the fertilizer package had a significant to very significant effect on plant growth, such as increasing plant height, increasing the number of primary branches, increasing stem diameter and number of leaves. The fertilizer package that gave the highest response to plant height, number of leaves, while the increase in stem diameter had the same effect. Primary branch augmentation is the most influential Ph package but is not significantly different from PC. The effect of fertilizer packages on changes in soil chemical properties is that pH is neutral, DHL is classified as low to very high, C-org is classified as high, N-tot is classified as medium, P-available and K-available is classified as very high. Implication: The effect of mixed organic, inorganic and biological fertilizers and probiotic POC provide the best response to plant growth and increase DHL in the soil.