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All Journal Indonesian Journal of Jamu
Christina Astutiningsih
Sekolah Tinggi Ilmu Farmasi, Yayasan Pharmasi Semarang, Semarang, Central Java, 50193
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Medicine & Pharmacology

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Bioassay-Guided and In Silico Identification of α-Amylase Inhibitors from Abelmoschus esculentus Fruit Christina Astutiningsih; Syifaur Rohmah; Novy Dwi Susilowati
Jurnal Jamu Indonesia Vol. 11 No. 3 (2026): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v11i3.447

Abstract

Diabetes mellitus is a metabolic disorder characterized by persistent hyperglycemia. Inhibition of α-amylase is one therapeutic strategy for controlling postprandial blood glucose levels. This study aimed to identify active compounds responsible for α-amylase inhibitory activity in Abelmoschus esculentus fruit using a bioassay-guided and in silico approach. Methanol extract, fractions, and subfractions were evaluated in vitro for α-amylase inhibition. The most active subfraction was characterized by LC–MS and further analyzed by molecular docking and ADMET prediction. Subfraction 2 showed the strongest inhibitory activity, with 74.2634 ± 4.7091% inhibition. LC–MS identified nine compounds, four of which showed favorable binding affinity and interaction patterns with α-amylase: 2-hydroxy-7-methoxycadalene, N-E-coumaroyl tyramine, homovanillic acid, and quercetin. These findings suggest that A. esculentus fruit contains bioactive compounds with potential α-amylase inhibitory activity.
Co-Authors Novy Dwi Susilowati Syifaur Rohmah